Amino acid dipepetide frequency for Mycobacterium phage Traaww1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.32AlaAla: 12.32 ± 1.242
1.013AlaCys: 1.013 ± 0.233
6.118AlaAsp: 6.118 ± 0.438
8.776AlaGlu: 8.776 ± 0.687
2.869AlaPhe: 2.869 ± 0.388
9.071AlaGly: 9.071 ± 0.97
2.278AlaHis: 2.278 ± 0.386
3.882AlaIle: 3.882 ± 0.454
4.725AlaLys: 4.725 ± 0.446
8.438AlaLeu: 8.438 ± 0.643
2.911AlaMet: 2.911 ± 0.335
3.08AlaAsn: 3.08 ± 0.533
4.725AlaPro: 4.725 ± 0.491
3.671AlaGln: 3.671 ± 0.334
6.54AlaArg: 6.54 ± 0.607
5.738AlaSer: 5.738 ± 0.575
6.371AlaThr: 6.371 ± 0.487
6.16AlaVal: 6.16 ± 0.477
1.519AlaTrp: 1.519 ± 0.247
2.742AlaTyr: 2.742 ± 0.383
0.0AlaXaa: 0.0 ± 0.0
Cys
1.139CysAla: 1.139 ± 0.252
0.295CysCys: 0.295 ± 0.118
0.633CysAsp: 0.633 ± 0.224
0.717CysGlu: 0.717 ± 0.194
0.295CysPhe: 0.295 ± 0.137
2.236CysGly: 2.236 ± 0.502
0.295CysHis: 0.295 ± 0.117
0.506CysIle: 0.506 ± 0.133
0.548CysLys: 0.548 ± 0.153
1.224CysLeu: 1.224 ± 0.257
0.084CysMet: 0.084 ± 0.063
0.506CysAsn: 0.506 ± 0.151
0.802CysPro: 0.802 ± 0.22
0.548CysGln: 0.548 ± 0.158
0.717CysArg: 0.717 ± 0.206
1.013CysSer: 1.013 ± 0.216
0.633CysThr: 0.633 ± 0.178
0.675CysVal: 0.675 ± 0.169
0.127CysTrp: 0.127 ± 0.084
0.38CysTyr: 0.38 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
5.78AspAla: 5.78 ± 0.661
1.181AspCys: 1.181 ± 0.272
3.882AspAsp: 3.882 ± 0.43
4.768AspGlu: 4.768 ± 0.615
1.519AspPhe: 1.519 ± 0.256
6.582AspGly: 6.582 ± 0.481
1.519AspHis: 1.519 ± 0.287
3.206AspIle: 3.206 ± 0.417
2.489AspLys: 2.489 ± 0.343
4.894AspLeu: 4.894 ± 0.441
1.35AspMet: 1.35 ± 0.261
1.561AspAsn: 1.561 ± 0.284
3.671AspPro: 3.671 ± 0.35
1.983AspGln: 1.983 ± 0.401
3.882AspArg: 3.882 ± 0.499
2.996AspSer: 2.996 ± 0.381
3.249AspThr: 3.249 ± 0.371
4.472AspVal: 4.472 ± 0.406
1.688AspTrp: 1.688 ± 0.237
2.7AspTyr: 2.7 ± 0.376
0.0AspXaa: 0.0 ± 0.0
Glu
6.582GluAla: 6.582 ± 0.663
1.097GluCys: 1.097 ± 0.258
4.936GluAsp: 4.936 ± 0.558
5.189GluGlu: 5.189 ± 0.622
2.574GluPhe: 2.574 ± 0.352
4.599GluGly: 4.599 ± 0.47
1.645GluHis: 1.645 ± 0.303
2.911GluIle: 2.911 ± 0.32
2.405GluLys: 2.405 ± 0.305
6.877GluLeu: 6.877 ± 0.597
1.434GluMet: 1.434 ± 0.239
2.152GluAsn: 2.152 ± 0.293
2.996GluPro: 2.996 ± 0.445
2.447GluGln: 2.447 ± 0.306
4.346GluArg: 4.346 ± 0.448
2.785GluSer: 2.785 ± 0.408
3.882GluThr: 3.882 ± 0.461
4.472GluVal: 4.472 ± 0.493
1.308GluTrp: 1.308 ± 0.23
2.278GluTyr: 2.278 ± 0.325
0.0GluXaa: 0.0 ± 0.0
Phe
2.574PheAla: 2.574 ± 0.385
0.591PheCys: 0.591 ± 0.172
2.616PheAsp: 2.616 ± 0.305
1.814PheGlu: 1.814 ± 0.257
0.886PhePhe: 0.886 ± 0.214
2.7PheGly: 2.7 ± 0.448
1.097PheHis: 1.097 ± 0.206
1.392PheIle: 1.392 ± 0.233
1.013PheLys: 1.013 ± 0.219
1.899PheLeu: 1.899 ± 0.265
0.633PheMet: 0.633 ± 0.154
0.97PheAsn: 0.97 ± 0.217
1.224PhePro: 1.224 ± 0.215
0.886PheGln: 0.886 ± 0.193
1.097PheArg: 1.097 ± 0.204
1.899PheSer: 1.899 ± 0.298
2.447PheThr: 2.447 ± 0.341
2.067PheVal: 2.067 ± 0.341
0.422PheTrp: 0.422 ± 0.125
0.886PheTyr: 0.886 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
8.058GlyAla: 8.058 ± 1.085
1.055GlyCys: 1.055 ± 0.256
5.4GlyAsp: 5.4 ± 0.478
5.485GlyGlu: 5.485 ± 0.429
2.025GlyPhe: 2.025 ± 0.266
9.155GlyGly: 9.155 ± 2.023
2.11GlyHis: 2.11 ± 0.244
3.924GlyIle: 3.924 ± 0.501
3.882GlyLys: 3.882 ± 0.427
6.329GlyLeu: 6.329 ± 0.452
2.278GlyMet: 2.278 ± 0.363
3.586GlyAsn: 3.586 ± 0.39
3.671GlyPro: 3.671 ± 0.435
2.489GlyGln: 2.489 ± 0.434
4.177GlyArg: 4.177 ± 0.424
5.189GlySer: 5.189 ± 0.737
6.666GlyThr: 6.666 ± 0.646
6.371GlyVal: 6.371 ± 0.449
1.688GlyTrp: 1.688 ± 0.277
3.375GlyTyr: 3.375 ± 0.361
0.0GlyXaa: 0.0 ± 0.0
His
2.194HisAla: 2.194 ± 0.294
0.422HisCys: 0.422 ± 0.141
1.561HisAsp: 1.561 ± 0.293
1.519HisGlu: 1.519 ± 0.281
0.675HisPhe: 0.675 ± 0.172
2.278HisGly: 2.278 ± 0.331
1.139HisHis: 1.139 ± 0.239
1.181HisIle: 1.181 ± 0.202
1.097HisLys: 1.097 ± 0.2
2.574HisLeu: 2.574 ± 0.39
0.591HisMet: 0.591 ± 0.134
1.013HisAsn: 1.013 ± 0.169
1.561HisPro: 1.561 ± 0.211
0.844HisGln: 0.844 ± 0.235
1.688HisArg: 1.688 ± 0.329
1.097HisSer: 1.097 ± 0.194
1.308HisThr: 1.308 ± 0.3
1.35HisVal: 1.35 ± 0.234
0.506HisTrp: 0.506 ± 0.141
1.181HisTyr: 1.181 ± 0.24
0.0HisXaa: 0.0 ± 0.0
Ile
4.557IleAla: 4.557 ± 0.419
0.464IleCys: 0.464 ± 0.126
3.249IleAsp: 3.249 ± 0.37
3.671IleGlu: 3.671 ± 0.45
1.097IlePhe: 1.097 ± 0.238
3.544IleGly: 3.544 ± 0.466
1.308IleHis: 1.308 ± 0.26
1.603IleIle: 1.603 ± 0.311
1.941IleLys: 1.941 ± 0.303
3.291IleLeu: 3.291 ± 0.476
0.928IleMet: 0.928 ± 0.219
1.856IleAsn: 1.856 ± 0.284
2.574IlePro: 2.574 ± 0.289
1.519IleGln: 1.519 ± 0.302
2.616IleArg: 2.616 ± 0.303
2.067IleSer: 2.067 ± 0.286
2.742IleThr: 2.742 ± 0.373
3.249IleVal: 3.249 ± 0.345
0.464IleTrp: 0.464 ± 0.137
1.097IleTyr: 1.097 ± 0.227
0.0IleXaa: 0.0 ± 0.0
Lys
5.063LysAla: 5.063 ± 0.434
0.802LysCys: 0.802 ± 0.19
2.616LysAsp: 2.616 ± 0.352
2.152LysGlu: 2.152 ± 0.293
1.139LysPhe: 1.139 ± 0.228
3.544LysGly: 3.544 ± 0.417
1.434LysHis: 1.434 ± 0.297
1.561LysIle: 1.561 ± 0.258
2.236LysLys: 2.236 ± 0.346
3.966LysLeu: 3.966 ± 0.344
1.139LysMet: 1.139 ± 0.231
1.519LysAsn: 1.519 ± 0.269
2.236LysPro: 2.236 ± 0.298
1.688LysGln: 1.688 ± 0.27
3.206LysArg: 3.206 ± 0.395
2.742LysSer: 2.742 ± 0.38
1.941LysThr: 1.941 ± 0.235
2.953LysVal: 2.953 ± 0.3
1.097LysTrp: 1.097 ± 0.18
1.013LysTyr: 1.013 ± 0.218
0.0LysXaa: 0.0 ± 0.0
Leu
9.282LeuAla: 9.282 ± 0.72
0.886LeuCys: 0.886 ± 0.219
4.725LeuAsp: 4.725 ± 0.393
4.388LeuGlu: 4.388 ± 0.468
2.11LeuPhe: 2.11 ± 0.347
5.949LeuGly: 5.949 ± 0.441
1.856LeuHis: 1.856 ± 0.276
3.797LeuIle: 3.797 ± 0.371
3.797LeuLys: 3.797 ± 0.432
7.088LeuLeu: 7.088 ± 0.633
2.363LeuMet: 2.363 ± 0.326
2.953LeuAsn: 2.953 ± 0.345
4.092LeuPro: 4.092 ± 0.452
3.122LeuGln: 3.122 ± 0.306
5.611LeuArg: 5.611 ± 0.463
5.147LeuSer: 5.147 ± 0.453
5.696LeuThr: 5.696 ± 0.5
4.936LeuVal: 4.936 ± 0.428
1.392LeuTrp: 1.392 ± 0.261
1.772LeuTyr: 1.772 ± 0.293
0.0LeuXaa: 0.0 ± 0.0
Met
2.32MetAla: 2.32 ± 0.323
0.295MetCys: 0.295 ± 0.122
1.392MetAsp: 1.392 ± 0.247
1.35MetGlu: 1.35 ± 0.267
0.675MetPhe: 0.675 ± 0.17
1.603MetGly: 1.603 ± 0.28
0.506MetHis: 0.506 ± 0.125
0.844MetIle: 0.844 ± 0.166
1.772MetLys: 1.772 ± 0.318
1.434MetLeu: 1.434 ± 0.209
0.38MetMet: 0.38 ± 0.117
1.013MetAsn: 1.013 ± 0.217
0.97MetPro: 0.97 ± 0.186
0.717MetGln: 0.717 ± 0.216
1.434MetArg: 1.434 ± 0.207
2.574MetSer: 2.574 ± 0.372
2.067MetThr: 2.067 ± 0.229
1.73MetVal: 1.73 ± 0.265
0.591MetTrp: 0.591 ± 0.138
0.295MetTyr: 0.295 ± 0.126
0.0MetXaa: 0.0 ± 0.0
Asn
3.08AsnAla: 3.08 ± 0.368
0.422AsnCys: 0.422 ± 0.163
2.278AsnAsp: 2.278 ± 0.315
1.561AsnGlu: 1.561 ± 0.212
0.928AsnPhe: 0.928 ± 0.191
4.514AsnGly: 4.514 ± 0.628
0.717AsnHis: 0.717 ± 0.167
1.097AsnIle: 1.097 ± 0.288
0.97AsnLys: 0.97 ± 0.176
3.544AsnLeu: 3.544 ± 0.453
0.464AsnMet: 0.464 ± 0.191
1.013AsnAsn: 1.013 ± 0.246
2.785AsnPro: 2.785 ± 0.351
1.139AsnGln: 1.139 ± 0.2
1.983AsnArg: 1.983 ± 0.262
1.688AsnSer: 1.688 ± 0.313
1.814AsnThr: 1.814 ± 0.345
2.194AsnVal: 2.194 ± 0.347
0.633AsnTrp: 0.633 ± 0.18
0.844AsnTyr: 0.844 ± 0.178
0.0AsnXaa: 0.0 ± 0.0
Pro
5.358ProAla: 5.358 ± 0.463
0.802ProCys: 0.802 ± 0.232
3.544ProAsp: 3.544 ± 0.364
3.755ProGlu: 3.755 ± 0.411
1.645ProPhe: 1.645 ± 0.256
4.936ProGly: 4.936 ± 0.711
1.73ProHis: 1.73 ± 0.304
2.531ProIle: 2.531 ± 0.368
2.616ProLys: 2.616 ± 0.448
3.544ProLeu: 3.544 ± 0.317
1.35ProMet: 1.35 ± 0.274
1.899ProAsn: 1.899 ± 0.252
3.249ProPro: 3.249 ± 0.423
1.772ProGln: 1.772 ± 0.267
2.574ProArg: 2.574 ± 0.333
2.531ProSer: 2.531 ± 0.269
3.164ProThr: 3.164 ± 0.317
3.249ProVal: 3.249 ± 0.358
1.139ProTrp: 1.139 ± 0.22
1.477ProTyr: 1.477 ± 0.245
0.0ProXaa: 0.0 ± 0.0
Gln
4.05GlnAla: 4.05 ± 0.385
0.295GlnCys: 0.295 ± 0.122
1.899GlnAsp: 1.899 ± 0.236
2.067GlnGlu: 2.067 ± 0.275
0.802GlnPhe: 0.802 ± 0.176
2.152GlnGly: 2.152 ± 0.323
0.464GlnHis: 0.464 ± 0.139
1.645GlnIle: 1.645 ± 0.227
1.688GlnLys: 1.688 ± 0.314
3.628GlnLeu: 3.628 ± 0.509
0.844GlnMet: 0.844 ± 0.187
0.928GlnAsn: 0.928 ± 0.163
1.899GlnPro: 1.899 ± 0.294
1.013GlnGln: 1.013 ± 0.21
2.278GlnArg: 2.278 ± 0.296
2.067GlnSer: 2.067 ± 0.265
1.645GlnThr: 1.645 ± 0.264
2.447GlnVal: 2.447 ± 0.322
0.886GlnTrp: 0.886 ± 0.219
1.055GlnTyr: 1.055 ± 0.193
0.0GlnXaa: 0.0 ± 0.0
Arg
6.919ArgAla: 6.919 ± 0.747
0.844ArgCys: 0.844 ± 0.234
3.797ArgAsp: 3.797 ± 0.47
3.839ArgGlu: 3.839 ± 0.404
1.814ArgPhe: 1.814 ± 0.295
3.713ArgGly: 3.713 ± 0.419
2.067ArgHis: 2.067 ± 0.368
2.405ArgIle: 2.405 ± 0.363
3.122ArgLys: 3.122 ± 0.411
4.81ArgLeu: 4.81 ± 0.62
2.025ArgMet: 2.025 ± 0.331
1.392ArgAsn: 1.392 ± 0.192
2.996ArgPro: 2.996 ± 0.311
1.941ArgGln: 1.941 ± 0.299
4.219ArgArg: 4.219 ± 0.627
3.038ArgSer: 3.038 ± 0.399
2.827ArgThr: 2.827 ± 0.322
4.514ArgVal: 4.514 ± 0.44
1.561ArgTrp: 1.561 ± 0.273
2.236ArgTyr: 2.236 ± 0.311
0.0ArgXaa: 0.0 ± 0.0
Ser
5.316SerAla: 5.316 ± 0.526
0.422SerCys: 0.422 ± 0.133
3.249SerAsp: 3.249 ± 0.332
3.671SerGlu: 3.671 ± 0.464
1.899SerPhe: 1.899 ± 0.35
5.738SerGly: 5.738 ± 0.758
1.392SerHis: 1.392 ± 0.249
2.067SerIle: 2.067 ± 0.379
2.194SerLys: 2.194 ± 0.328
4.81SerLeu: 4.81 ± 0.525
1.181SerMet: 1.181 ± 0.183
1.941SerAsn: 1.941 ± 0.264
2.742SerPro: 2.742 ± 0.324
1.73SerGln: 1.73 ± 0.316
3.46SerArg: 3.46 ± 0.465
3.46SerSer: 3.46 ± 0.391
3.417SerThr: 3.417 ± 0.396
3.755SerVal: 3.755 ± 0.407
1.308SerTrp: 1.308 ± 0.24
1.603SerTyr: 1.603 ± 0.262
0.0SerXaa: 0.0 ± 0.0
Thr
6.455ThrAla: 6.455 ± 0.585
0.844ThrCys: 0.844 ± 0.229
3.333ThrAsp: 3.333 ± 0.417
3.755ThrGlu: 3.755 ± 0.352
2.194ThrPhe: 2.194 ± 0.278
5.527ThrGly: 5.527 ± 0.682
1.35ThrHis: 1.35 ± 0.23
3.08ThrIle: 3.08 ± 0.338
2.236ThrLys: 2.236 ± 0.323
4.641ThrLeu: 4.641 ± 0.487
1.434ThrMet: 1.434 ± 0.263
2.067ThrAsn: 2.067 ± 0.294
4.557ThrPro: 4.557 ± 0.469
1.856ThrGln: 1.856 ± 0.311
2.785ThrArg: 2.785 ± 0.391
2.574ThrSer: 2.574 ± 0.307
3.08ThrThr: 3.08 ± 0.38
6.624ThrVal: 6.624 ± 0.56
1.266ThrTrp: 1.266 ± 0.316
2.025ThrTyr: 2.025 ± 0.215
0.0ThrXaa: 0.0 ± 0.0
Val
7.552ValAla: 7.552 ± 0.566
0.928ValCys: 0.928 ± 0.216
5.063ValAsp: 5.063 ± 0.498
5.864ValGlu: 5.864 ± 0.63
2.32ValPhe: 2.32 ± 0.315
4.388ValGly: 4.388 ± 0.368
1.603ValHis: 1.603 ± 0.244
3.882ValIle: 3.882 ± 0.381
3.502ValLys: 3.502 ± 0.43
4.008ValLeu: 4.008 ± 0.445
1.477ValMet: 1.477 ± 0.23
2.658ValAsn: 2.658 ± 0.304
3.46ValPro: 3.46 ± 0.352
2.405ValGln: 2.405 ± 0.303
4.008ValArg: 4.008 ± 0.406
4.261ValSer: 4.261 ± 0.43
5.274ValThr: 5.274 ± 0.653
5.949ValVal: 5.949 ± 0.691
1.013ValTrp: 1.013 ± 0.207
1.983ValTyr: 1.983 ± 0.289
0.0ValXaa: 0.0 ± 0.0
Trp
1.645TrpAla: 1.645 ± 0.255
0.295TrpCys: 0.295 ± 0.116
1.434TrpAsp: 1.434 ± 0.206
0.675TrpGlu: 0.675 ± 0.168
0.802TrpPhe: 0.802 ± 0.22
1.35TrpGly: 1.35 ± 0.282
0.675TrpHis: 0.675 ± 0.192
1.013TrpIle: 1.013 ± 0.182
0.717TrpLys: 0.717 ± 0.144
1.645TrpLeu: 1.645 ± 0.266
0.464TrpMet: 0.464 ± 0.155
0.717TrpAsn: 0.717 ± 0.178
0.928TrpPro: 0.928 ± 0.216
0.928TrpGln: 0.928 ± 0.213
1.097TrpArg: 1.097 ± 0.246
1.181TrpSer: 1.181 ± 0.253
1.139TrpThr: 1.139 ± 0.232
1.941TrpVal: 1.941 ± 0.322
0.338TrpTrp: 0.338 ± 0.115
0.633TrpTyr: 0.633 ± 0.214
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.7TyrAla: 2.7 ± 0.376
0.422TyrCys: 0.422 ± 0.154
1.645TyrAsp: 1.645 ± 0.343
1.899TyrGlu: 1.899 ± 0.286
0.844TyrPhe: 0.844 ± 0.206
3.333TyrGly: 3.333 ± 0.388
0.591TyrHis: 0.591 ± 0.15
1.308TyrIle: 1.308 ± 0.219
1.097TyrLys: 1.097 ± 0.255
2.32TyrLeu: 2.32 ± 0.292
0.591TyrMet: 0.591 ± 0.145
0.844TyrAsn: 0.844 ± 0.183
1.73TyrPro: 1.73 ± 0.303
1.055TyrGln: 1.055 ± 0.189
2.363TyrArg: 2.363 ± 0.305
1.308TyrSer: 1.308 ± 0.21
2.32TyrThr: 2.32 ± 0.242
2.531TyrVal: 2.531 ± 0.37
0.675TyrTrp: 0.675 ± 0.182
0.591TyrTyr: 0.591 ± 0.198
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 142 proteins (23703 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski