alpha proteobacterium HIMB114

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; Pelagibacteraceae; unclassified Pelagibacteraceae

Average proteome isoelectric point is 7.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1321 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0RNL6|D0RNL6_9PROT 50S ribosomal protein L1 OS=alpha proteobacterium HIMB114 OX=684719 GN=rplA PE=3 SV=1
MM1 pKa = 7.59KK2 pKa = 10.46KK3 pKa = 10.35LLATTAIVSVSFASVALAEE22 pKa = 4.38VTVKK26 pKa = 10.77GSIEE30 pKa = 3.64QTINSRR36 pKa = 11.84SYY38 pKa = 9.63NKK40 pKa = 10.06AANEE44 pKa = 4.06HH45 pKa = 6.7KK46 pKa = 10.14GTGSMGQEE54 pKa = 3.93TNLTVSSSGEE64 pKa = 3.9LDD66 pKa = 2.96NGLSIKK72 pKa = 10.63GSFRR76 pKa = 11.84SEE78 pKa = 4.28DD79 pKa = 3.26GSVDD83 pKa = 3.05HH84 pKa = 7.19SSIKK88 pKa = 10.57VSGDD92 pKa = 3.1TLGFEE97 pKa = 4.51IGADD101 pKa = 3.32TGSTIHH107 pKa = 5.63TQINPTVGDD116 pKa = 3.9SVWFVTGAQGDD127 pKa = 4.15DD128 pKa = 3.26GFTTYY133 pKa = 10.12EE134 pKa = 3.85AHH136 pKa = 7.08DD137 pKa = 3.95VQHH140 pKa = 6.98IGIDD144 pKa = 3.43AKK146 pKa = 11.02LGGMTAAVNYY156 pKa = 9.97APSNNGISAGDD167 pKa = 3.85SNKK170 pKa = 9.53TDD172 pKa = 3.28AGGSATEE179 pKa = 4.01MLLKK183 pKa = 10.4GSIMEE188 pKa = 4.47GVNFLIGQEE197 pKa = 4.15KK198 pKa = 10.05IEE200 pKa = 4.21AANSGGSEE208 pKa = 4.15EE209 pKa = 4.37TEE211 pKa = 3.56KK212 pKa = 10.24TYY214 pKa = 10.7QVSYY218 pKa = 10.71SAGQFALGASIRR230 pKa = 11.84DD231 pKa = 3.66FDD233 pKa = 6.24DD234 pKa = 4.72GDD236 pKa = 3.91STATAGTTDD245 pKa = 3.29EE246 pKa = 4.38VTYY249 pKa = 9.21LTATYY254 pKa = 10.43AVNDD258 pKa = 3.81QVSLGIQSVTAEE270 pKa = 4.18SEE272 pKa = 4.42GTGTSATDD280 pKa = 3.38EE281 pKa = 4.24EE282 pKa = 4.95TLGISLGYY290 pKa = 9.76NLGPIGVEE298 pKa = 3.69VMYY301 pKa = 11.16AEE303 pKa = 4.61TDD305 pKa = 3.49NLAHH309 pKa = 6.8SNADD313 pKa = 3.39DD314 pKa = 3.9VEE316 pKa = 4.66GVQIRR321 pKa = 11.84TVYY324 pKa = 10.65KK325 pKa = 10.04FF326 pKa = 3.04

Molecular weight: 33.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0RNS5|D0RNS5_9PROT 5-aminolevulinate synthase OS=alpha proteobacterium HIMB114 OX=684719 GN=HIMB114_00004490 PE=3 SV=1
MM1 pKa = 6.66VTRR4 pKa = 11.84KK5 pKa = 10.28SFTRR9 pKa = 11.84PVTKK13 pKa = 10.2TSNPQPKK20 pKa = 9.66NKK22 pKa = 9.55KK23 pKa = 9.38SKK25 pKa = 10.77DD26 pKa = 3.48WIIWAAWADD35 pKa = 3.5RR36 pKa = 11.84VTFEE40 pKa = 5.24DD41 pKa = 3.24IKK43 pKa = 11.07RR44 pKa = 11.84EE45 pKa = 3.97TGRR48 pKa = 11.84TEE50 pKa = 3.94SEE52 pKa = 4.15VIKK55 pKa = 10.68IMRR58 pKa = 11.84STLKK62 pKa = 10.43PSSFRR67 pKa = 11.84LWRR70 pKa = 11.84KK71 pKa = 8.93RR72 pKa = 11.84VHH74 pKa = 5.88TKK76 pKa = 10.29SIKK79 pKa = 8.91NLKK82 pKa = 9.62KK83 pKa = 10.46FRR85 pKa = 11.84EE86 pKa = 4.24DD87 pKa = 3.02RR88 pKa = 11.84QKK90 pKa = 10.64IRR92 pKa = 11.84NKK94 pKa = 10.3SYY96 pKa = 11.29KK97 pKa = 10.37NYY99 pKa = 10.72LL100 pKa = 3.4

Molecular weight: 12.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1321

0

1321

397694

30

1502

301.1

34.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.423 ± 0.073

1.03 ± 0.02

5.191 ± 0.056

5.894 ± 0.068

5.582 ± 0.082

6.13 ± 0.069

1.581 ± 0.03

9.552 ± 0.081

10.909 ± 0.098

9.445 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.072 ± 0.032

6.698 ± 0.086

3.148 ± 0.039

2.839 ± 0.035

3.139 ± 0.048

6.832 ± 0.052

4.63 ± 0.042

5.652 ± 0.067

0.833 ± 0.024

3.422 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski