Porcine reproductive and respiratory syndrome virus (strain HB-1) (PRRSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Arnidovirineae; Arteriviridae; unclassified Arteriviridae; Porcine reproductive and respiratory syndrome virus

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8B910|Q8B910_PRRSB GP2a OS=Porcine reproductive and respiratory syndrome virus (strain HB-1) OX=300563 GN=ORF2a PE=2 SV=1
MM1 pKa = 7.73GSMQSLFDD9 pKa = 4.68KK10 pKa = 10.64IGQLFVDD17 pKa = 4.8AFTEE21 pKa = 4.33FLVSIVDD28 pKa = 3.88IIIFLAILLGFTIAGWLVVFCIRR51 pKa = 11.84LVCSAVLRR59 pKa = 11.84ARR61 pKa = 11.84PTVHH65 pKa = 7.4PEE67 pKa = 3.51QLQKK71 pKa = 10.73ISS73 pKa = 3.26

Molecular weight: 8.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q8B912|RPOA_PRRSB Replicase polyprotein 1ab OS=Porcine reproductive and respiratory syndrome virus (strain HB-1) OX=300563 GN=rep PE=2 SV=3
MM1 pKa = 7.7KK2 pKa = 9.93WGLCKK7 pKa = 10.65ASLTKK12 pKa = 10.48LANFLWMLSRR22 pKa = 11.84NFWCPLLISSYY33 pKa = 10.4FWPFCLASPSPVGWWSSASDD53 pKa = 3.33WFAPRR58 pKa = 11.84YY59 pKa = 8.69SVRR62 pKa = 11.84ALPFTLSNYY71 pKa = 9.01RR72 pKa = 11.84RR73 pKa = 11.84SHH75 pKa = 5.68EE76 pKa = 4.27AFLSQCQVDD85 pKa = 3.42IPTWGVKK92 pKa = 10.06HH93 pKa = 5.91PLGVLWHH100 pKa = 6.71HH101 pKa = 6.4KK102 pKa = 10.21VSTLIDD108 pKa = 3.53EE109 pKa = 4.49MVSRR113 pKa = 11.84RR114 pKa = 11.84MYY116 pKa = 10.63RR117 pKa = 11.84IMEE120 pKa = 4.37KK121 pKa = 10.39AGQAAWKK128 pKa = 9.81QVVSEE133 pKa = 4.27ATLSRR138 pKa = 11.84ISSLDD143 pKa = 3.43VVAHH147 pKa = 6.05FQHH150 pKa = 6.68LAAIEE155 pKa = 4.4AEE157 pKa = 4.33TCKK160 pKa = 10.76YY161 pKa = 9.79LASRR165 pKa = 11.84LPMLHH170 pKa = 6.56NLRR173 pKa = 11.84MTGSNVTIVYY183 pKa = 10.36NSTSNQVFAIFPTPGSRR200 pKa = 11.84PKK202 pKa = 10.85LHH204 pKa = 7.62DD205 pKa = 3.61FQQWLIAVHH214 pKa = 6.43SSIFSSVAASCTLFVVLWLRR234 pKa = 11.84IPMLRR239 pKa = 11.84SVFGFRR245 pKa = 11.84WLGAIFLLNSRR256 pKa = 4.67

Molecular weight: 29.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

1

7

7425

73

3961

1060.7

116.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.269 ± 0.117

3.003 ± 0.165

4.269 ± 0.379

4.646 ± 0.427

4.485 ± 0.604

7.596 ± 0.722

2.505 ± 0.233

3.771 ± 0.415

4.108 ± 0.315

10.357 ± 0.326

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.128

3.111 ± 0.102

7.205 ± 0.808

3.152 ± 0.107

5.333 ± 0.168

7.34 ± 0.79

5.899 ± 0.174

8.498 ± 0.195

1.845 ± 0.244

2.519 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski