Gordonia phage KatherineG
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A160DGS6|A0A160DGS6_9CAUD Uncharacterized protein OS=Gordonia phage KatherineG OX=1838070 GN=79 PE=4 SV=1
MM1 pKa = 7.44 AFSYY5 pKa = 10.65 TDD7 pKa = 3.28 EE8 pKa = 6.22 DD9 pKa = 4.16 GDD11 pKa = 4.29 LIEE14 pKa = 4.52 VARR17 pKa = 11.84 SSQHH21 pKa = 4.6 NTLLTSVVDD30 pKa = 3.91 PDD32 pKa = 4.39 GNSHH36 pKa = 6.08 SVYY39 pKa = 10.29 IPFSEE44 pKa = 3.98 IPRR47 pKa = 11.84 LIEE50 pKa = 4.78 DD51 pKa = 3.84 LAGYY55 pKa = 10.09 LGG57 pKa = 3.71
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 4.037
IPC_protein 3.897
Toseland 3.719
ProMoST 4.088
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.859
Rodwell 3.745
Grimsley 3.63
Solomon 3.872
Lehninger 3.834
Nozaki 4.037
DTASelect 4.24
Thurlkill 3.783
EMBOSS 3.859
Sillero 4.037
Patrickios 1.926
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.914
Protein with the highest isoelectric point:
>tr|A0A160DGD6|A0A160DGD6_9CAUD Holin OS=Gordonia phage KatherineG OX=1838070 GN=13 PE=4 SV=1
MM1 pKa = 7.26 SWAGSTRR8 pKa = 11.84 RR9 pKa = 11.84 QRR11 pKa = 11.84 LPEE14 pKa = 4.27 DD15 pKa = 3.05 WEE17 pKa = 4.28 LNYY20 pKa = 10.43 RR21 pKa = 11.84 LPVLSRR27 pKa = 11.84 DD28 pKa = 3.11 RR29 pKa = 11.84 WLCQINGPGCKK40 pKa = 9.07 RR41 pKa = 11.84 AATDD45 pKa = 2.86 VDD47 pKa = 4.22 HH48 pKa = 7.59 IKK50 pKa = 10.86 RR51 pKa = 11.84 GDD53 pKa = 3.28 NHH55 pKa = 6.82 DD56 pKa = 3.9 RR57 pKa = 11.84 SNLHH61 pKa = 6.15 AVCSVCHH68 pKa = 5.87 NKK70 pKa = 9.89 KK71 pKa = 10.49 SSAEE75 pKa = 3.97 GNQAKK80 pKa = 10.41 AKK82 pKa = 10.42 LKK84 pKa = 9.98 AQRR87 pKa = 11.84 KK88 pKa = 8.52 RR89 pKa = 11.84 PPEE92 pKa = 3.71 RR93 pKa = 11.84 HH94 pKa = 5.7 PGRR97 pKa = 11.84 RR98 pKa = 3.38
Molecular weight: 11.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.472
IPC_protein 9.867
Toseland 10.745
ProMoST 10.599
Dawson 10.804
Bjellqvist 10.467
Wikipedia 10.965
Rodwell 11.082
Grimsley 10.818
Solomon 10.921
Lehninger 10.906
Nozaki 10.745
DTASelect 10.452
Thurlkill 10.73
EMBOSS 11.14
Sillero 10.745
Patrickios 10.847
IPC_peptide 10.935
IPC2_peptide 9.619
IPC2.peptide.svr19 8.665
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
98
0
98
16052
32
728
163.8
18.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.164 ± 0.288
0.916 ± 0.14
6.535 ± 0.245
6.952 ± 0.249
3.464 ± 0.208
8.117 ± 0.362
2.112 ± 0.165
4.984 ± 0.194
4.666 ± 0.251
8.049 ± 0.307
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.486 ± 0.127
3.433 ± 0.16
5.326 ± 0.203
3.258 ± 0.135
5.999 ± 0.27
5.887 ± 0.264
6.579 ± 0.198
6.965 ± 0.203
1.956 ± 0.124
3.152 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here