Klebsiella phage vB_KpnM_KB57

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vequintavirinae; Mydovirus; Klebsiella virus KB57

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 245 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S1S1D9|A0A0S1S1D9_9CAUD Putative nicotinamide phosphoribosyl transferase OS=Klebsiella phage vB_KpnM_KB57 OX=1719140 GN=KB57_093 PE=3 SV=1
MM1 pKa = 8.34DD2 pKa = 4.22DD3 pKa = 3.64TGFLLLGMLITAFLILIPYY22 pKa = 10.48GMMLYY27 pKa = 10.62VSIGPVAKK35 pKa = 10.41KK36 pKa = 10.44DD37 pKa = 3.57DD38 pKa = 3.93CC39 pKa = 5.9

Molecular weight: 4.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S1S2A2|A0A0S1S2A2_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnM_KB57 OX=1719140 GN=KB57_010 PE=4 SV=1
MM1 pKa = 6.76TAKK4 pKa = 10.2SRR6 pKa = 11.84NFEE9 pKa = 4.02KK10 pKa = 8.6MTSQRR15 pKa = 11.84DD16 pKa = 3.22RR17 pKa = 11.84LAFGEE22 pKa = 4.31TNEE25 pKa = 4.4RR26 pKa = 11.84RR27 pKa = 11.84EE28 pKa = 4.36RR29 pKa = 11.84NNKK32 pKa = 6.67RR33 pKa = 11.84HH34 pKa = 5.47KK35 pKa = 9.59PEE37 pKa = 5.1RR38 pKa = 11.84IAVQWSPDD46 pKa = 3.34QYY48 pKa = 11.54DD49 pKa = 3.42PKK51 pKa = 10.81LNKK54 pKa = 9.85RR55 pKa = 11.84LADD58 pKa = 3.82RR59 pKa = 11.84KK60 pKa = 10.6RR61 pKa = 11.84NLQRR65 pKa = 11.84YY66 pKa = 7.91AVAGEE71 pKa = 3.91

Molecular weight: 8.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

245

0

245

42170

39

880

172.1

19.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.228 ± 0.229

1.224 ± 0.096

6.514 ± 0.123

6.782 ± 0.193

4.195 ± 0.123

7.097 ± 0.171

1.869 ± 0.091

6.026 ± 0.122

7.06 ± 0.23

7.97 ± 0.146

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.931 ± 0.101

4.743 ± 0.132

3.538 ± 0.117

3.272 ± 0.11

4.923 ± 0.149

5.952 ± 0.145

5.914 ± 0.265

7.143 ± 0.155

1.624 ± 0.079

3.996 ± 0.1
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski