Mycobacterium phage Melissauren88

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1PC10|A0A2S1PC10_9CAUD Uncharacterized protein OS=Mycobacterium phage Melissauren88 OX=2163593 GN=48 PE=4 SV=1
MM1 pKa = 7.29NLVDD5 pKa = 4.32RR6 pKa = 11.84LNARR10 pKa = 11.84FNNVIHH16 pKa = 7.66DD17 pKa = 4.05GLALVGAVVDD27 pKa = 3.85PWLAKK32 pKa = 10.34LEE34 pKa = 4.03RR35 pKa = 11.84QAMSNALGRR44 pKa = 11.84DD45 pKa = 3.45FGLDD49 pKa = 3.54YY50 pKa = 11.62ADD52 pKa = 4.08GLAAAEE58 pKa = 4.26ADD60 pKa = 4.07VEE62 pKa = 4.47VHH64 pKa = 6.47EE65 pKa = 5.15PAPCVCGHH73 pKa = 6.3LRR75 pKa = 11.84EE76 pKa = 3.99DD77 pKa = 3.83HH78 pKa = 6.06YY79 pKa = 11.45RR80 pKa = 11.84VGGFIDD86 pKa = 3.93PCSKK90 pKa = 10.56CDD92 pKa = 4.56CLDD95 pKa = 3.66LLYY98 pKa = 10.75EE99 pKa = 4.42SSAEE103 pKa = 3.91LWASADD109 pKa = 3.67PSPVSVGDD117 pKa = 3.67TGPGAGMVPPPPPAPGPSKK136 pKa = 9.67CTCPTVEE143 pKa = 4.74CEE145 pKa = 5.28LLAEE149 pKa = 5.26DD150 pKa = 4.11ICDD153 pKa = 3.51EE154 pKa = 4.3AEE156 pKa = 3.98EE157 pKa = 5.19AEE159 pKa = 4.37LTGDD163 pKa = 3.27DD164 pKa = 3.53WMSLGEE170 pKa = 4.13YY171 pKa = 10.5LEE173 pKa = 4.39TATAEE178 pKa = 3.95EE179 pKa = 4.43LAAMRR184 pKa = 11.84QQRR187 pKa = 11.84EE188 pKa = 3.89VSEE191 pKa = 5.42DD192 pKa = 3.69DD193 pKa = 3.13LTMRR197 pKa = 11.84IADD200 pKa = 3.54LHH202 pKa = 5.89GWSVPSIVDD211 pKa = 3.32SRR213 pKa = 11.84IARR216 pKa = 11.84ALLEE220 pKa = 4.58TYY222 pKa = 10.26HH223 pKa = 5.58ITPRR227 pKa = 3.57

Molecular weight: 24.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1PC24|A0A2S1PC24_9CAUD Uncharacterized protein OS=Mycobacterium phage Melissauren88 OX=2163593 GN=73 PE=4 SV=1
MM1 pKa = 7.67SNGNRR6 pKa = 11.84LTNEE10 pKa = 3.77QVKK13 pKa = 9.91MILAMTRR20 pKa = 11.84DD21 pKa = 4.12GFSARR26 pKa = 11.84HH27 pKa = 4.83IAEE30 pKa = 4.16VVGCSPRR37 pKa = 11.84TVTRR41 pKa = 11.84VRR43 pKa = 11.84AAADD47 pKa = 3.34ARR49 pKa = 11.84VMNPRR54 pKa = 11.84RR55 pKa = 11.84FTPLTADD62 pKa = 3.02QLEE65 pKa = 4.26FAEE68 pKa = 4.73YY69 pKa = 10.38LLEE72 pKa = 5.99DD73 pKa = 4.19GAPYY77 pKa = 10.49QEE79 pKa = 4.4VARR82 pKa = 11.84TLGVSRR88 pKa = 11.84TTIEE92 pKa = 3.47KK93 pKa = 10.16HH94 pKa = 5.02FPGRR98 pKa = 11.84AWTKK102 pKa = 9.06RR103 pKa = 11.84QAAEE107 pKa = 4.06FTALLKK113 pKa = 10.58KK114 pKa = 10.4FRR116 pKa = 11.84RR117 pKa = 11.84LEE119 pKa = 3.94ASS121 pKa = 3.05

Molecular weight: 13.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

17203

30

1174

181.1

19.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.626 ± 0.469

1.186 ± 0.164

6.76 ± 0.236

5.662 ± 0.305

3.017 ± 0.194

8.981 ± 0.599

2.273 ± 0.205

4.412 ± 0.228

3.168 ± 0.176

7.208 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.314 ± 0.142

3.366 ± 0.161

6.307 ± 0.215

3.47 ± 0.173

6.697 ± 0.365

5.999 ± 0.262

6.522 ± 0.251

7.121 ± 0.26

2.412 ± 0.176

2.5 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski