Amino acid dipepetide frequency for Mycobacterium phage GS4E

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.142AlaAla: 11.142 ± 0.987
0.396AlaCys: 0.396 ± 0.142
4.153AlaAsp: 4.153 ± 0.495
6.922AlaGlu: 6.922 ± 0.759
3.89AlaPhe: 3.89 ± 0.575
7.845AlaGly: 7.845 ± 0.873
1.78AlaHis: 1.78 ± 0.348
4.681AlaIle: 4.681 ± 0.757
5.208AlaLys: 5.208 ± 0.574
9.428AlaLeu: 9.428 ± 1.161
2.505AlaMet: 2.505 ± 0.428
3.428AlaAsn: 3.428 ± 0.651
5.67AlaPro: 5.67 ± 0.713
3.56AlaGln: 3.56 ± 0.539
6.131AlaArg: 6.131 ± 0.776
5.208AlaSer: 5.208 ± 0.673
5.67AlaThr: 5.67 ± 0.673
7.186AlaVal: 7.186 ± 0.626
1.253AlaTrp: 1.253 ± 0.354
2.505AlaTyr: 2.505 ± 0.423
0.0AlaXaa: 0.0 ± 0.0
Cys
0.725CysAla: 0.725 ± 0.222
0.132CysCys: 0.132 ± 0.154
0.593CysAsp: 0.593 ± 0.211
0.461CysGlu: 0.461 ± 0.169
0.264CysPhe: 0.264 ± 0.145
0.923CysGly: 0.923 ± 0.247
0.33CysHis: 0.33 ± 0.169
0.264CysIle: 0.264 ± 0.14
0.659CysLys: 0.659 ± 0.211
0.725CysLeu: 0.725 ± 0.265
0.264CysMet: 0.264 ± 0.161
0.527CysAsn: 0.527 ± 0.161
0.461CysPro: 0.461 ± 0.197
0.132CysGln: 0.132 ± 0.1
0.396CysArg: 0.396 ± 0.149
0.527CysSer: 0.527 ± 0.211
0.593CysThr: 0.593 ± 0.153
0.527CysVal: 0.527 ± 0.166
0.264CysTrp: 0.264 ± 0.155
0.527CysTyr: 0.527 ± 0.163
0.0CysXaa: 0.0 ± 0.0
Asp
7.186AspAla: 7.186 ± 0.913
0.593AspCys: 0.593 ± 0.2
3.956AspAsp: 3.956 ± 0.654
5.076AspGlu: 5.076 ± 0.697
2.703AspPhe: 2.703 ± 0.499
5.406AspGly: 5.406 ± 0.609
1.846AspHis: 1.846 ± 0.452
3.23AspIle: 3.23 ± 0.441
2.439AspLys: 2.439 ± 0.36
5.67AspLeu: 5.67 ± 0.67
1.912AspMet: 1.912 ± 0.352
1.648AspAsn: 1.648 ± 0.371
4.747AspPro: 4.747 ± 0.764
1.978AspGln: 1.978 ± 0.371
2.901AspArg: 2.901 ± 0.445
2.439AspSer: 2.439 ± 0.327
2.373AspThr: 2.373 ± 0.43
4.153AspVal: 4.153 ± 0.529
1.45AspTrp: 1.45 ± 0.329
2.176AspTyr: 2.176 ± 0.44
0.0AspXaa: 0.0 ± 0.0
Glu
7.714GluAla: 7.714 ± 0.822
0.264GluCys: 0.264 ± 0.115
5.076GluAsp: 5.076 ± 0.692
5.208GluGlu: 5.208 ± 0.774
3.099GluPhe: 3.099 ± 0.539
5.274GluGly: 5.274 ± 0.62
1.846GluHis: 1.846 ± 0.409
3.692GluIle: 3.692 ± 0.513
2.967GluLys: 2.967 ± 0.44
6.725GluLeu: 6.725 ± 0.725
2.505GluMet: 2.505 ± 0.332
2.307GluAsn: 2.307 ± 0.435
2.571GluPro: 2.571 ± 0.46
2.703GluGln: 2.703 ± 0.42
4.417GluArg: 4.417 ± 0.498
2.967GluSer: 2.967 ± 0.484
3.626GluThr: 3.626 ± 0.45
4.879GluVal: 4.879 ± 0.689
1.253GluTrp: 1.253 ± 0.29
2.044GluTyr: 2.044 ± 0.333
0.0GluXaa: 0.0 ± 0.0
Phe
3.23PheAla: 3.23 ± 0.488
0.33PheCys: 0.33 ± 0.142
2.769PheAsp: 2.769 ± 0.576
1.846PheGlu: 1.846 ± 0.319
0.659PhePhe: 0.659 ± 0.269
3.428PheGly: 3.428 ± 0.547
1.055PheHis: 1.055 ± 0.34
1.45PheIle: 1.45 ± 0.334
1.714PheLys: 1.714 ± 0.323
2.901PheLeu: 2.901 ± 0.631
0.593PheMet: 0.593 ± 0.228
1.582PheAsn: 1.582 ± 0.306
1.978PhePro: 1.978 ± 0.37
1.121PheGln: 1.121 ± 0.265
1.846PheArg: 1.846 ± 0.445
1.648PheSer: 1.648 ± 0.286
1.912PheThr: 1.912 ± 0.297
2.11PheVal: 2.11 ± 0.377
0.33PheTrp: 0.33 ± 0.169
0.791PheTyr: 0.791 ± 0.229
0.066PheXaa: 0.066 ± 0.06
Gly
6.197GlyAla: 6.197 ± 0.864
0.923GlyCys: 0.923 ± 0.232
5.604GlyAsp: 5.604 ± 0.652
5.274GlyGlu: 5.274 ± 0.612
3.296GlyPhe: 3.296 ± 0.542
7.648GlyGly: 7.648 ± 1.481
1.319GlyHis: 1.319 ± 0.305
4.285GlyIle: 4.285 ± 0.707
4.219GlyLys: 4.219 ± 0.529
6.461GlyLeu: 6.461 ± 0.748
1.714GlyMet: 1.714 ± 0.318
3.165GlyAsn: 3.165 ± 0.437
3.56GlyPro: 3.56 ± 0.423
2.835GlyGln: 2.835 ± 0.484
3.89GlyArg: 3.89 ± 0.401
3.824GlySer: 3.824 ± 0.72
5.472GlyThr: 5.472 ± 0.849
6.857GlyVal: 6.857 ± 0.741
1.253GlyTrp: 1.253 ± 0.28
2.373GlyTyr: 2.373 ± 0.373
0.0GlyXaa: 0.0 ± 0.0
His
1.253HisAla: 1.253 ± 0.277
0.264HisCys: 0.264 ± 0.116
1.582HisAsp: 1.582 ± 0.365
1.45HisGlu: 1.45 ± 0.325
0.461HisPhe: 0.461 ± 0.185
1.78HisGly: 1.78 ± 0.483
0.396HisHis: 0.396 ± 0.152
1.187HisIle: 1.187 ± 0.255
1.121HisLys: 1.121 ± 0.275
1.648HisLeu: 1.648 ± 0.366
0.264HisMet: 0.264 ± 0.148
0.593HisAsn: 0.593 ± 0.195
1.319HisPro: 1.319 ± 0.328
0.791HisGln: 0.791 ± 0.225
1.582HisArg: 1.582 ± 0.437
0.923HisSer: 0.923 ± 0.283
1.384HisThr: 1.384 ± 0.315
0.923HisVal: 0.923 ± 0.286
0.461HisTrp: 0.461 ± 0.168
0.527HisTyr: 0.527 ± 0.268
0.0HisXaa: 0.0 ± 0.0
Ile
5.076IleAla: 5.076 ± 0.555
0.659IleCys: 0.659 ± 0.236
3.758IleAsp: 3.758 ± 0.59
4.813IleGlu: 4.813 ± 0.586
1.45IlePhe: 1.45 ± 0.317
4.417IleGly: 4.417 ± 0.668
1.055IleHis: 1.055 ± 0.233
1.978IleIle: 1.978 ± 0.357
2.703IleLys: 2.703 ± 0.525
3.89IleLeu: 3.89 ± 0.497
0.791IleMet: 0.791 ± 0.224
2.505IleAsn: 2.505 ± 0.489
3.626IlePro: 3.626 ± 0.428
1.648IleGln: 1.648 ± 0.425
2.967IleArg: 2.967 ± 0.499
2.637IleSer: 2.637 ± 0.338
3.033IleThr: 3.033 ± 0.432
2.571IleVal: 2.571 ± 0.38
0.725IleTrp: 0.725 ± 0.206
1.187IleTyr: 1.187 ± 0.25
0.0IleXaa: 0.0 ± 0.0
Lys
5.274LysAla: 5.274 ± 0.637
0.264LysCys: 0.264 ± 0.127
2.901LysAsp: 2.901 ± 0.46
2.439LysGlu: 2.439 ± 0.378
1.187LysPhe: 1.187 ± 0.267
3.692LysGly: 3.692 ± 0.533
0.461LysHis: 0.461 ± 0.223
2.505LysIle: 2.505 ± 0.459
2.571LysLys: 2.571 ± 0.578
5.076LysLeu: 5.076 ± 0.604
0.989LysMet: 0.989 ± 0.285
1.384LysAsn: 1.384 ± 0.308
2.835LysPro: 2.835 ± 0.418
2.044LysGln: 2.044 ± 0.356
3.23LysArg: 3.23 ± 0.45
2.769LysSer: 2.769 ± 0.466
3.033LysThr: 3.033 ± 0.4
3.89LysVal: 3.89 ± 0.474
0.593LysTrp: 0.593 ± 0.202
1.319LysTyr: 1.319 ± 0.232
0.0LysXaa: 0.0 ± 0.0
Leu
8.768LeuAla: 8.768 ± 0.825
0.659LeuCys: 0.659 ± 0.197
5.934LeuAsp: 5.934 ± 0.652
6.988LeuGlu: 6.988 ± 0.779
2.769LeuPhe: 2.769 ± 0.377
5.208LeuGly: 5.208 ± 0.877
1.78LeuHis: 1.78 ± 0.362
4.022LeuIle: 4.022 ± 0.536
3.692LeuLys: 3.692 ± 0.475
5.67LeuLeu: 5.67 ± 0.665
2.571LeuMet: 2.571 ± 0.601
2.571LeuAsn: 2.571 ± 0.352
5.142LeuPro: 5.142 ± 0.547
2.967LeuGln: 2.967 ± 0.476
5.604LeuArg: 5.604 ± 0.688
5.34LeuSer: 5.34 ± 0.688
6.131LeuThr: 6.131 ± 1.038
5.076LeuVal: 5.076 ± 0.676
1.319LeuTrp: 1.319 ± 0.223
1.912LeuTyr: 1.912 ± 0.388
0.0LeuXaa: 0.0 ± 0.0
Met
2.901MetAla: 2.901 ± 0.401
0.066MetCys: 0.066 ± 0.071
1.187MetAsp: 1.187 ± 0.337
1.253MetGlu: 1.253 ± 0.286
0.527MetPhe: 0.527 ± 0.191
1.846MetGly: 1.846 ± 0.342
0.33MetHis: 0.33 ± 0.135
1.78MetIle: 1.78 ± 0.35
1.384MetLys: 1.384 ± 0.283
1.45MetLeu: 1.45 ± 0.344
0.593MetMet: 0.593 ± 0.179
0.659MetAsn: 0.659 ± 0.214
1.648MetPro: 1.648 ± 0.341
1.121MetGln: 1.121 ± 0.301
1.582MetArg: 1.582 ± 0.345
2.307MetSer: 2.307 ± 0.322
2.505MetThr: 2.505 ± 0.401
0.791MetVal: 0.791 ± 0.202
0.198MetTrp: 0.198 ± 0.108
0.791MetTyr: 0.791 ± 0.237
0.0MetXaa: 0.0 ± 0.0
Asn
3.165AsnAla: 3.165 ± 0.521
0.593AsnCys: 0.593 ± 0.196
2.307AsnAsp: 2.307 ± 0.396
2.439AsnGlu: 2.439 ± 0.41
0.791AsnPhe: 0.791 ± 0.251
2.769AsnGly: 2.769 ± 0.499
0.989AsnHis: 0.989 ± 0.222
1.187AsnIle: 1.187 ± 0.266
1.187AsnLys: 1.187 ± 0.251
3.428AsnLeu: 3.428 ± 0.581
0.593AsnMet: 0.593 ± 0.189
0.725AsnAsn: 0.725 ± 0.262
2.505AsnPro: 2.505 ± 0.354
1.055AsnGln: 1.055 ± 0.291
2.176AsnArg: 2.176 ± 0.445
1.78AsnSer: 1.78 ± 0.381
2.044AsnThr: 2.044 ± 0.402
1.912AsnVal: 1.912 ± 0.398
1.055AsnTrp: 1.055 ± 0.255
0.791AsnTyr: 0.791 ± 0.218
0.0AsnXaa: 0.0 ± 0.0
Pro
5.076ProAla: 5.076 ± 0.575
0.396ProCys: 0.396 ± 0.173
4.153ProAsp: 4.153 ± 0.613
5.208ProGlu: 5.208 ± 0.67
1.78ProPhe: 1.78 ± 0.373
4.351ProGly: 4.351 ± 0.529
0.923ProHis: 0.923 ± 0.292
2.967ProIle: 2.967 ± 0.376
2.637ProLys: 2.637 ± 0.463
3.099ProLeu: 3.099 ± 0.513
1.384ProMet: 1.384 ± 0.315
2.044ProAsn: 2.044 ± 0.32
2.176ProPro: 2.176 ± 0.507
1.912ProGln: 1.912 ± 0.385
3.362ProArg: 3.362 ± 0.442
2.835ProSer: 2.835 ± 0.347
4.219ProThr: 4.219 ± 0.633
3.956ProVal: 3.956 ± 0.532
1.253ProTrp: 1.253 ± 0.398
1.582ProTyr: 1.582 ± 0.351
0.0ProXaa: 0.0 ± 0.0
Gln
4.681GlnAla: 4.681 ± 0.69
0.264GlnCys: 0.264 ± 0.143
1.319GlnAsp: 1.319 ± 0.276
1.978GlnGlu: 1.978 ± 0.365
1.055GlnPhe: 1.055 ± 0.301
2.769GlnGly: 2.769 ± 0.367
0.461GlnHis: 0.461 ± 0.166
2.769GlnIle: 2.769 ± 0.402
1.78GlnLys: 1.78 ± 0.389
3.758GlnLeu: 3.758 ± 0.702
1.055GlnMet: 1.055 ± 0.212
0.725GlnAsn: 0.725 ± 0.213
1.516GlnPro: 1.516 ± 0.481
2.769GlnGln: 2.769 ± 0.528
2.11GlnArg: 2.11 ± 0.427
1.846GlnSer: 1.846 ± 0.388
2.439GlnThr: 2.439 ± 0.43
3.033GlnVal: 3.033 ± 0.402
0.725GlnTrp: 0.725 ± 0.26
0.791GlnTyr: 0.791 ± 0.201
0.0GlnXaa: 0.0 ± 0.0
Arg
4.945ArgAla: 4.945 ± 0.711
0.923ArgCys: 0.923 ± 0.286
4.022ArgAsp: 4.022 ± 0.619
4.747ArgGlu: 4.747 ± 0.662
2.373ArgPhe: 2.373 ± 0.504
3.758ArgGly: 3.758 ± 0.535
1.187ArgHis: 1.187 ± 0.323
3.956ArgIle: 3.956 ± 0.56
3.165ArgLys: 3.165 ± 0.49
4.945ArgLeu: 4.945 ± 0.626
1.582ArgMet: 1.582 ± 0.326
2.11ArgAsn: 2.11 ± 0.396
2.505ArgPro: 2.505 ± 0.421
2.373ArgGln: 2.373 ± 0.435
5.142ArgArg: 5.142 ± 0.67
2.769ArgSer: 2.769 ± 0.488
2.703ArgThr: 2.703 ± 0.47
3.626ArgVal: 3.626 ± 0.447
1.319ArgTrp: 1.319 ± 0.31
2.571ArgTyr: 2.571 ± 0.385
0.0ArgXaa: 0.0 ± 0.0
Ser
4.879SerAla: 4.879 ± 0.582
0.527SerCys: 0.527 ± 0.194
2.835SerAsp: 2.835 ± 0.429
3.362SerGlu: 3.362 ± 0.513
1.78SerPhe: 1.78 ± 0.336
4.879SerGly: 4.879 ± 0.471
0.923SerHis: 0.923 ± 0.287
2.637SerIle: 2.637 ± 0.384
3.099SerLys: 3.099 ± 0.714
4.219SerLeu: 4.219 ± 0.598
1.714SerMet: 1.714 ± 0.276
1.055SerAsn: 1.055 ± 0.267
2.703SerPro: 2.703 ± 0.336
2.176SerGln: 2.176 ± 0.334
3.033SerArg: 3.033 ± 0.402
2.373SerSer: 2.373 ± 0.423
3.428SerThr: 3.428 ± 0.579
3.362SerVal: 3.362 ± 0.537
0.989SerTrp: 0.989 ± 0.297
1.45SerTyr: 1.45 ± 0.315
0.0SerXaa: 0.0 ± 0.0
Thr
5.868ThrAla: 5.868 ± 0.593
0.593ThrCys: 0.593 ± 0.174
3.824ThrAsp: 3.824 ± 0.601
3.428ThrGlu: 3.428 ± 0.459
1.846ThrPhe: 1.846 ± 0.335
5.406ThrGly: 5.406 ± 0.803
1.055ThrHis: 1.055 ± 0.277
2.835ThrIle: 2.835 ± 0.376
2.835ThrLys: 2.835 ± 0.48
5.934ThrLeu: 5.934 ± 0.725
1.319ThrMet: 1.319 ± 0.252
1.846ThrAsn: 1.846 ± 0.519
4.483ThrPro: 4.483 ± 0.583
2.11ThrGln: 2.11 ± 0.397
3.099ThrArg: 3.099 ± 0.489
2.637ThrSer: 2.637 ± 0.452
3.099ThrThr: 3.099 ± 0.522
5.011ThrVal: 5.011 ± 0.605
1.187ThrTrp: 1.187 ± 0.26
2.11ThrTyr: 2.11 ± 0.35
0.0ThrXaa: 0.0 ± 0.0
Val
6.065ValAla: 6.065 ± 0.808
0.725ValCys: 0.725 ± 0.231
4.681ValAsp: 4.681 ± 0.555
5.011ValGlu: 5.011 ± 0.599
1.78ValPhe: 1.78 ± 0.427
4.945ValGly: 4.945 ± 0.439
1.253ValHis: 1.253 ± 0.306
3.494ValIle: 3.494 ± 0.623
3.824ValLys: 3.824 ± 0.437
5.406ValLeu: 5.406 ± 0.636
1.45ValMet: 1.45 ± 0.337
2.901ValAsn: 2.901 ± 0.525
3.099ValPro: 3.099 ± 0.479
2.439ValGln: 2.439 ± 0.47
4.285ValArg: 4.285 ± 0.623
3.956ValSer: 3.956 ± 0.554
4.285ValThr: 4.285 ± 0.373
5.538ValVal: 5.538 ± 0.56
1.384ValTrp: 1.384 ± 0.323
2.11ValTyr: 2.11 ± 0.377
0.0ValXaa: 0.0 ± 0.0
Trp
1.582TrpAla: 1.582 ± 0.388
0.33TrpCys: 0.33 ± 0.18
1.121TrpAsp: 1.121 ± 0.262
1.187TrpGlu: 1.187 ± 0.321
0.593TrpPhe: 0.593 ± 0.187
1.516TrpGly: 1.516 ± 0.327
0.527TrpHis: 0.527 ± 0.225
0.923TrpIle: 0.923 ± 0.258
0.396TrpLys: 0.396 ± 0.161
1.055TrpLeu: 1.055 ± 0.231
0.396TrpMet: 0.396 ± 0.16
0.659TrpAsn: 0.659 ± 0.205
0.989TrpPro: 0.989 ± 0.252
1.187TrpGln: 1.187 ± 0.264
1.187TrpArg: 1.187 ± 0.291
0.857TrpSer: 0.857 ± 0.263
1.187TrpThr: 1.187 ± 0.232
1.516TrpVal: 1.516 ± 0.234
0.461TrpTrp: 0.461 ± 0.165
0.396TrpTyr: 0.396 ± 0.214
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.835TyrAla: 2.835 ± 0.377
0.396TyrCys: 0.396 ± 0.151
2.044TyrAsp: 2.044 ± 0.314
2.11TyrGlu: 2.11 ± 0.383
1.055TyrPhe: 1.055 ± 0.226
2.307TyrGly: 2.307 ± 0.439
0.396TyrHis: 0.396 ± 0.17
1.648TyrIle: 1.648 ± 0.281
0.857TyrLys: 0.857 ± 0.204
2.637TyrLeu: 2.637 ± 0.374
0.659TyrMet: 0.659 ± 0.228
1.055TyrAsn: 1.055 ± 0.26
1.78TyrPro: 1.78 ± 0.348
0.923TyrGln: 0.923 ± 0.221
1.78TyrArg: 1.78 ± 0.374
1.78TyrSer: 1.78 ± 0.357
1.384TyrThr: 1.384 ± 0.303
1.714TyrVal: 1.714 ± 0.412
0.593TyrTrp: 0.593 ± 0.269
0.659TyrTyr: 0.659 ± 0.203
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.066XaaAsp: 0.066 ± 0.06
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 88 proteins (15169 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski