Caulobacter phage RW

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514AAY4|A0A514AAY4_9CAUD VRR-NUC domain-containing protein OS=Caulobacter phage RW OX=2591034 GN=RW_GP058 PE=4 SV=1
MM1 pKa = 7.24IHH3 pKa = 6.89VDD5 pKa = 4.24EE6 pKa = 5.54LPGGYY11 pKa = 10.13DD12 pKa = 3.76ADD14 pKa = 4.27DD15 pKa = 3.48VDD17 pKa = 5.82AIIMVCDD24 pKa = 4.32GPGVCPNKK32 pKa = 10.42GDD34 pKa = 3.64AAQAAGCPLCRR45 pKa = 11.84TVFVMADD52 pKa = 2.96GFEE55 pKa = 4.49FVLEE59 pKa = 4.28PTEE62 pKa = 4.08HH63 pKa = 6.63

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514AAY1|A0A514AAY1_9CAUD Uncharacterized protein OS=Caulobacter phage RW OX=2591034 GN=RW_GP023 PE=4 SV=1
MM1 pKa = 6.58TRR3 pKa = 11.84SVARR7 pKa = 11.84AWSEE11 pKa = 3.51EE12 pKa = 3.84DD13 pKa = 4.31DD14 pKa = 4.81AVLLKK19 pKa = 10.68DD20 pKa = 3.26WARR23 pKa = 11.84GRR25 pKa = 11.84SASVIGMRR33 pKa = 11.84LGRR36 pKa = 11.84SRR38 pKa = 11.84CAVLGRR44 pKa = 11.84LYY46 pKa = 10.85RR47 pKa = 11.84LGALNEE53 pKa = 4.03DD54 pKa = 3.96RR55 pKa = 11.84KK56 pKa = 10.74AVAPEE61 pKa = 3.72RR62 pKa = 11.84LPAHH66 pKa = 6.53RR67 pKa = 11.84PFGNKK72 pKa = 8.93HH73 pKa = 5.38ASKK76 pKa = 10.78DD77 pKa = 3.29LWTEE81 pKa = 3.86SLLTEE86 pKa = 4.09KK87 pKa = 10.1WADD90 pKa = 3.54RR91 pKa = 11.84KK92 pKa = 10.68ARR94 pKa = 11.84LAKK97 pKa = 10.0EE98 pKa = 4.02RR99 pKa = 11.84AQGATQQ105 pKa = 3.1

Molecular weight: 11.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

18264

30

960

188.3

20.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.401 ± 0.387

0.975 ± 0.118

6.307 ± 0.279

6.045 ± 0.343

3.088 ± 0.169

7.988 ± 0.387

1.818 ± 0.186

3.887 ± 0.144

4.265 ± 0.252

8.596 ± 0.286

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.283 ± 0.13

3.17 ± 0.192

5.316 ± 0.277

3.729 ± 0.252

6.844 ± 0.309

5.579 ± 0.356

6.455 ± 0.425

6.855 ± 0.233

1.703 ± 0.131

2.694 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski