Capybara microvirus Cap1_SP_115
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W4F8|A0A4P8W4F8_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_115 OX=2584808 PE=4 SV=1
MM1 pKa = 7.36 ATNPLQPANHH11 pKa = 7.08 AEE13 pKa = 4.21 TLNPEE18 pKa = 4.06 AQANNPSIRR27 pKa = 11.84 FRR29 pKa = 11.84 SPSNTFYY36 pKa = 10.96 LPKK39 pKa = 9.99 RR40 pKa = 11.84 HH41 pKa = 6.39 YY42 pKa = 9.21 DD43 pKa = 3.49 TIRR46 pKa = 11.84 FGEE49 pKa = 4.01 YY50 pKa = 9.48 RR51 pKa = 11.84 PVYY54 pKa = 8.82 NLEE57 pKa = 4.26 MIPNGRR63 pKa = 11.84 YY64 pKa = 8.25 MLQDD68 pKa = 3.32 ANRR71 pKa = 11.84 IEE73 pKa = 4.49 SFTFASRR80 pKa = 11.84 FMEE83 pKa = 4.27 GLSYY87 pKa = 10.61 TKK89 pKa = 10.36 DD90 pKa = 3.36 YY91 pKa = 10.96 FQVPFSAILPRR102 pKa = 11.84 NWEE105 pKa = 3.94 KK106 pKa = 10.57 IYY108 pKa = 10.63 RR109 pKa = 11.84 NPAFGDD115 pKa = 3.87 DD116 pKa = 3.95 VPDD119 pKa = 5.19 DD120 pKa = 4.32 SMSLINFQKK129 pKa = 10.49 LFNDD133 pKa = 3.19 IASRR137 pKa = 11.84 LISQPSSLQDD147 pKa = 3.36 LTFMLILPYY156 pKa = 10.46 LFSRR160 pKa = 11.84 GSLLDD165 pKa = 3.38 SLGFPVVDD173 pKa = 5.14 ISFLDD178 pKa = 3.78 DD179 pKa = 3.49 NGEE182 pKa = 3.77 YY183 pKa = 10.5 DD184 pKa = 4.43 IIQEE188 pKa = 4.5 IIDD191 pKa = 3.81 TSGSFQLKK199 pKa = 10.3 SPISSIPNFFYY210 pKa = 10.61 KK211 pKa = 10.73 VSDD214 pKa = 3.17 ASTFNILINDD224 pKa = 4.39 FLLNGYY230 pKa = 9.22 SIISGPSDD238 pKa = 3.53 FEE240 pKa = 4.27 SLRR243 pKa = 11.84 PLSAQWKK250 pKa = 9.91 AKK252 pKa = 9.35 FQALASSLAGSEE264 pKa = 4.53 LISVASLYY272 pKa = 10.56 AYY274 pKa = 9.95 KK275 pKa = 9.37 LTCAKK280 pKa = 10.39 FYY282 pKa = 10.89 TNDD285 pKa = 3.85 NIDD288 pKa = 4.88 PIYY291 pKa = 10.14 MSQLWIDD298 pKa = 3.91 SVQSLLSVSDD308 pKa = 3.54 DD309 pKa = 3.61 TQPTFNYY316 pKa = 10.13 NGSKK320 pKa = 10.4 YY321 pKa = 10.5 LYY323 pKa = 10.12 DD324 pKa = 3.9 VLSLHH329 pKa = 6.74 YY330 pKa = 10.38 INEE333 pKa = 4.32 KK334 pKa = 10.57 LFSLLDD340 pKa = 3.56 SDD342 pKa = 4.2 YY343 pKa = 10.77 TNALIALFGPEE354 pKa = 4.4 HH355 pKa = 7.48 VLIQQDD361 pKa = 3.93 FFLSARR367 pKa = 11.84 SRR369 pKa = 11.84 PLAVGNVDD377 pKa = 2.82 IHH379 pKa = 7.09 VNTSSQSVSAIDD391 pKa = 3.37 VTKK394 pKa = 10.89 NITIQRR400 pKa = 11.84 YY401 pKa = 8.23 LNAVNRR407 pKa = 11.84 LGNKK411 pKa = 6.29 ITSYY415 pKa = 10.31 MEE417 pKa = 5.29 GIFSTQPQEE426 pKa = 4.27 CPEE429 pKa = 4.19 PVLLTSLRR437 pKa = 11.84 YY438 pKa = 9.53 DD439 pKa = 3.06 VSTNQTTNTANDD451 pKa = 3.54 QGNVTSLAQSNGSKK465 pKa = 10.23 YY466 pKa = 10.9 AFDD469 pKa = 4.08 IFCSEE474 pKa = 3.94 PSILLGIAHH483 pKa = 6.64 FAIPKK488 pKa = 8.85 VYY490 pKa = 10.08 RR491 pKa = 11.84 FAMDD495 pKa = 2.97 MCKK498 pKa = 10.21 YY499 pKa = 9.51 HH500 pKa = 7.3 ANRR503 pKa = 11.84 FQFFNPMLEE512 pKa = 4.28 NIGDD516 pKa = 3.83 DD517 pKa = 4.82 GIPVQCFDD525 pKa = 4.13 FRR527 pKa = 11.84 MPKK530 pKa = 10.3 NLDD533 pKa = 3.45 KK534 pKa = 11.36 PNFGYY539 pKa = 8.42 ITRR542 pKa = 11.84 FGEE545 pKa = 4.13 YY546 pKa = 10.45 KK547 pKa = 9.87 LTFDD551 pKa = 3.84 EE552 pKa = 4.28 AHH554 pKa = 6.56 GGFMTNPLQSWIYY567 pKa = 10.37 QGLDD571 pKa = 3.08 ALDD574 pKa = 4.16 AQSVINPFVIRR585 pKa = 11.84 YY586 pKa = 8.5 RR587 pKa = 11.84 VGSMDD592 pKa = 3.65 YY593 pKa = 10.24 LYY595 pKa = 11.1 SAVSPYY601 pKa = 9.93 GPQWYY606 pKa = 8.93 YY607 pKa = 11.22 HH608 pKa = 6.08 FIVRR612 pKa = 11.84 NEE614 pKa = 4.11 TNLTANLPLSYY625 pKa = 11.0 NPGILL630 pKa = 3.62
Molecular weight: 71.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.707
IPC2_protein 4.889
IPC_protein 4.863
Toseland 4.736
ProMoST 5.016
Dawson 4.863
Bjellqvist 4.991
Wikipedia 4.774
Rodwell 4.736
Grimsley 4.66
Solomon 4.863
Lehninger 4.813
Nozaki 4.978
DTASelect 5.207
Thurlkill 4.762
EMBOSS 4.8
Sillero 5.029
Patrickios 3.032
IPC_peptide 4.863
IPC2_peptide 5.016
IPC2.peptide.svr19 4.972
Protein with the highest isoelectric point:
>tr|A0A4P8W7U3|A0A4P8W7U3_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_115 OX=2584808 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 9.93 KK3 pKa = 10.08 VCVFDD8 pKa = 4.83 CPPGMYY14 pKa = 10.27 YY15 pKa = 10.32 EE16 pKa = 4.47 WLPHH20 pKa = 5.74 SCIKK24 pKa = 10.42 YY25 pKa = 8.78 VRR27 pKa = 11.84 KK28 pKa = 9.97 VRR30 pKa = 11.84 NNSNKK35 pKa = 8.94 FHH37 pKa = 7.6 AIRR40 pKa = 11.84 YY41 pKa = 5.74 TRR43 pKa = 11.84 WLDD46 pKa = 3.25 GLIYY50 pKa = 9.8 TEE52 pKa = 4.21 KK53 pKa = 9.78 TVCSLCFLVPLGVNYY68 pKa = 10.46 NPYY71 pKa = 10.03 KK72 pKa = 10.46 QLFF75 pKa = 3.65
Molecular weight: 9.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.787
IPC2_protein 8.697
IPC_protein 8.668
Toseland 8.931
ProMoST 8.99
Dawson 9.341
Bjellqvist 9.341
Wikipedia 9.502
Rodwell 9.428
Grimsley 9.297
Solomon 9.458
Lehninger 9.428
Nozaki 9.458
DTASelect 9.194
Thurlkill 9.253
EMBOSS 9.458
Sillero 9.472
Patrickios 2.32
IPC_peptide 9.443
IPC2_peptide 8.448
IPC2.peptide.svr19 7.931
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1702
75
630
283.7
32.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.463 ± 1.747
1.234 ± 0.655
6.169 ± 0.404
3.995 ± 0.477
6.639 ± 0.501
4.407 ± 0.406
1.528 ± 0.319
6.169 ± 0.592
5.464 ± 1.042
9.166 ± 0.824
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.821 ± 0.181
7.873 ± 0.384
4.759 ± 0.717
4.407 ± 1.058
4.054 ± 0.501
10.106 ± 1.064
4.113 ± 0.372
4.7 ± 0.47
0.881 ± 0.233
6.052 ± 0.406
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here