Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Betaherpesvirinae; Cytomegalovirus; Human betaherpesvirus 5

Average proteome isoelectric point is 7.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 202 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P16794|NEC1_HCMVA Nuclear egress protein 1 OS=Human cytomegalovirus (strain AD169) OX=10360 GN=NEC1 PE=1 SV=1
MM1 pKa = 7.29SWAKK5 pKa = 10.28QRR7 pKa = 11.84VPFLDD12 pKa = 4.84DD13 pKa = 4.63DD14 pKa = 4.86DD15 pKa = 6.59GEE17 pKa = 4.49EE18 pKa = 4.22EE19 pKa = 5.34NDD21 pKa = 3.6VQDD24 pKa = 5.45DD25 pKa = 3.68VDD27 pKa = 4.53SPVPTRR33 pKa = 11.84PLVIDD38 pKa = 4.18EE39 pKa = 4.73DD40 pKa = 4.27AEE42 pKa = 4.29PAAGTSGGLEE52 pKa = 4.0GGGGDD57 pKa = 5.46DD58 pKa = 4.41EE59 pKa = 6.62DD60 pKa = 6.77GEE62 pKa = 4.95DD63 pKa = 3.85GHH65 pKa = 7.16ALPDD69 pKa = 4.37LDD71 pKa = 6.43DD72 pKa = 5.74DD73 pKa = 5.41LLLQFEE79 pKa = 4.52PMLPRR84 pKa = 11.84VYY86 pKa = 11.02DD87 pKa = 3.96LLLPSLDD94 pKa = 3.31ARR96 pKa = 11.84LNFVNAGQKK105 pKa = 9.24YY106 pKa = 8.82AAFLKK111 pKa = 10.45YY112 pKa = 10.31VHH114 pKa = 7.07GDD116 pKa = 3.42CATCSHH122 pKa = 6.6GEE124 pKa = 3.71ILRR127 pKa = 11.84EE128 pKa = 3.89KK129 pKa = 9.12TQLLTAIVSKK139 pKa = 10.96LMDD142 pKa = 3.6INGILEE148 pKa = 4.65GKK150 pKa = 10.17DD151 pKa = 3.19EE152 pKa = 4.29SAPGKK157 pKa = 10.38

Molecular weight: 16.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P16739|UL119_HCMVA Viral Fc-gamma receptor-like protein UL119 OS=Human cytomegalovirus (strain AD169) OX=10360 GN=UL119/UL118 PE=2 SV=2
MM1 pKa = 7.29IHH3 pKa = 7.51DD4 pKa = 3.76YY5 pKa = 10.12HH6 pKa = 5.92WRR8 pKa = 11.84RR9 pKa = 11.84GKK11 pKa = 9.75RR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 9.85LIVMMEE20 pKa = 4.01TRR22 pKa = 11.84MMIIMMTIKK31 pKa = 10.08PPHH34 pKa = 6.91DD35 pKa = 3.29IYY37 pKa = 11.05RR38 pKa = 11.84EE39 pKa = 4.09QQNHH43 pKa = 6.62HH44 pKa = 7.26DD45 pKa = 4.46DD46 pKa = 3.52EE47 pKa = 4.97TKK49 pKa = 10.55RR50 pKa = 11.84STKK53 pKa = 9.94HH54 pKa = 5.96CVTATHH60 pKa = 6.92PWPRR64 pKa = 11.84PAASCFRR71 pKa = 11.84CPLRR75 pKa = 11.84GGHH78 pKa = 5.55HH79 pKa = 6.6RR80 pKa = 11.84RR81 pKa = 11.84RR82 pKa = 11.84PACALPHH89 pKa = 6.16GWSVMNSCSS98 pKa = 3.31

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

190

12

202

69046

60

2241

341.8

38.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.953 ± 0.173

2.484 ± 0.101

4.803 ± 0.11

5.243 ± 0.13

3.834 ± 0.127

6.36 ± 0.223

3.269 ± 0.098

3.435 ± 0.126

2.992 ± 0.119

10.064 ± 0.241

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.003 ± 0.069

3.217 ± 0.121

6.176 ± 0.197

3.592 ± 0.164

8.15 ± 0.188

7.756 ± 0.241

6.735 ± 0.201

7.365 ± 0.145

1.372 ± 0.075

3.201 ± 0.095
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski