Amino acid dipepetide frequency for Clostridium phage vB_CpeS-CP51

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.027AlaAla: 2.027 ± 0.49
0.253AlaCys: 0.253 ± 0.108
3.293AlaAsp: 3.293 ± 0.468
3.209AlaGlu: 3.209 ± 0.511
1.773AlaPhe: 1.773 ± 0.339
2.364AlaGly: 2.364 ± 0.467
0.507AlaHis: 0.507 ± 0.174
7.178AlaIle: 7.178 ± 1.054
6.502AlaLys: 6.502 ± 1.016
4.476AlaLeu: 4.476 ± 0.656
1.182AlaMet: 1.182 ± 0.443
3.969AlaAsn: 3.969 ± 1.122
1.604AlaPro: 1.604 ± 0.506
1.858AlaGln: 1.858 ± 0.337
2.111AlaArg: 2.111 ± 0.433
3.716AlaSer: 3.716 ± 0.676
4.053AlaThr: 4.053 ± 0.757
2.364AlaVal: 2.364 ± 0.479
0.844AlaTrp: 0.844 ± 0.241
1.351AlaTyr: 1.351 ± 0.299
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.084CysCys: 0.084 ± 0.087
0.422CysAsp: 0.422 ± 0.182
0.844CysGlu: 0.844 ± 0.363
0.507CysPhe: 0.507 ± 0.215
0.76CysGly: 0.76 ± 0.239
0.084CysHis: 0.084 ± 0.115
0.929CysIle: 0.929 ± 0.314
1.52CysLys: 1.52 ± 0.31
1.098CysLeu: 1.098 ± 0.364
0.253CysMet: 0.253 ± 0.155
0.169CysAsn: 0.169 ± 0.104
0.507CysPro: 0.507 ± 0.217
0.169CysGln: 0.169 ± 0.13
0.253CysArg: 0.253 ± 0.123
0.844CysSer: 0.844 ± 0.29
0.76CysThr: 0.76 ± 0.281
0.76CysVal: 0.76 ± 0.206
0.084CysTrp: 0.084 ± 0.063
0.591CysTyr: 0.591 ± 0.207
0.0CysXaa: 0.0 ± 0.0
Asp
2.364AspAla: 2.364 ± 0.373
0.507AspCys: 0.507 ± 0.174
3.124AspAsp: 3.124 ± 0.526
3.969AspGlu: 3.969 ± 0.876
2.702AspPhe: 2.702 ± 0.498
4.138AspGly: 4.138 ± 0.683
0.591AspHis: 0.591 ± 0.185
7.262AspIle: 7.262 ± 0.685
5.658AspLys: 5.658 ± 0.636
4.813AspLeu: 4.813 ± 0.464
1.267AspMet: 1.267 ± 0.462
3.8AspAsn: 3.8 ± 0.602
0.929AspPro: 0.929 ± 0.266
0.591AspGln: 0.591 ± 0.222
1.773AspArg: 1.773 ± 0.37
4.391AspSer: 4.391 ± 0.626
2.702AspThr: 2.702 ± 0.449
2.533AspVal: 2.533 ± 0.474
0.929AspTrp: 0.929 ± 0.23
2.364AspTyr: 2.364 ± 0.488
0.0AspXaa: 0.0 ± 0.0
Glu
4.307GluAla: 4.307 ± 0.61
1.098GluCys: 1.098 ± 0.36
3.04GluAsp: 3.04 ± 0.691
7.853GluGlu: 7.853 ± 1.111
2.871GluPhe: 2.871 ± 0.531
4.391GluGly: 4.391 ± 0.562
1.267GluHis: 1.267 ± 0.373
6.333GluIle: 6.333 ± 0.69
7.853GluLys: 7.853 ± 1.215
6.925GluLeu: 6.925 ± 0.978
2.111GluMet: 2.111 ± 0.541
6.418GluAsn: 6.418 ± 1.038
1.013GluPro: 1.013 ± 0.332
3.124GluGln: 3.124 ± 0.544
3.8GluArg: 3.8 ± 0.834
3.8GluSer: 3.8 ± 0.558
3.462GluThr: 3.462 ± 0.546
5.067GluVal: 5.067 ± 0.678
1.098GluTrp: 1.098 ± 0.245
3.716GluTyr: 3.716 ± 0.534
0.0GluXaa: 0.0 ± 0.0
Phe
1.436PheAla: 1.436 ± 0.288
0.253PheCys: 0.253 ± 0.134
2.449PheAsp: 2.449 ± 0.506
2.28PheGlu: 2.28 ± 0.471
1.182PhePhe: 1.182 ± 0.386
1.52PheGly: 1.52 ± 0.356
0.338PheHis: 0.338 ± 0.161
3.378PheIle: 3.378 ± 0.59
5.151PheLys: 5.151 ± 0.535
2.787PheLeu: 2.787 ± 0.486
1.182PheMet: 1.182 ± 0.318
3.293PheAsn: 3.293 ± 0.495
1.267PhePro: 1.267 ± 0.453
1.013PheGln: 1.013 ± 0.287
1.267PheArg: 1.267 ± 0.285
2.702PheSer: 2.702 ± 0.335
2.871PheThr: 2.871 ± 0.455
2.027PheVal: 2.027 ± 0.488
0.169PheTrp: 0.169 ± 0.094
1.858PheTyr: 1.858 ± 0.384
0.0PheXaa: 0.0 ± 0.0
Gly
3.462GlyAla: 3.462 ± 0.529
0.338GlyCys: 0.338 ± 0.144
4.053GlyAsp: 4.053 ± 0.553
3.547GlyGlu: 3.547 ± 0.437
2.787GlyPhe: 2.787 ± 0.324
2.702GlyGly: 2.702 ± 0.868
0.676GlyHis: 0.676 ± 0.202
5.573GlyIle: 5.573 ± 0.69
6.333GlyLys: 6.333 ± 0.779
4.138GlyLeu: 4.138 ± 0.603
0.591GlyMet: 0.591 ± 0.219
4.56GlyAsn: 4.56 ± 0.69
0.422GlyPro: 0.422 ± 0.184
1.858GlyGln: 1.858 ± 0.397
1.942GlyArg: 1.942 ± 0.366
2.533GlySer: 2.533 ± 0.49
3.969GlyThr: 3.969 ± 0.795
3.209GlyVal: 3.209 ± 0.597
1.098GlyTrp: 1.098 ± 0.252
2.449GlyTyr: 2.449 ± 0.413
0.0GlyXaa: 0.0 ± 0.0
His
0.422HisAla: 0.422 ± 0.155
0.084HisCys: 0.084 ± 0.086
0.338HisAsp: 0.338 ± 0.182
0.929HisGlu: 0.929 ± 0.242
0.422HisPhe: 0.422 ± 0.174
0.76HisGly: 0.76 ± 0.223
0.0HisHis: 0.0 ± 0.0
1.351HisIle: 1.351 ± 0.345
1.013HisLys: 1.013 ± 0.339
0.676HisLeu: 0.676 ± 0.229
0.253HisMet: 0.253 ± 0.142
0.676HisAsn: 0.676 ± 0.269
0.253HisPro: 0.253 ± 0.154
0.084HisGln: 0.084 ± 0.1
0.76HisArg: 0.76 ± 0.222
0.676HisSer: 0.676 ± 0.269
0.422HisThr: 0.422 ± 0.159
0.676HisVal: 0.676 ± 0.256
0.169HisTrp: 0.169 ± 0.094
0.676HisTyr: 0.676 ± 0.268
0.0HisXaa: 0.0 ± 0.0
Ile
5.658IleAla: 5.658 ± 0.753
0.929IleCys: 0.929 ± 0.276
5.827IleAsp: 5.827 ± 0.546
7.938IleGlu: 7.938 ± 0.666
3.293IlePhe: 3.293 ± 0.717
4.813IleGly: 4.813 ± 0.925
0.929IleHis: 0.929 ± 0.306
6.08IleIle: 6.08 ± 0.831
11.4IleLys: 11.4 ± 1.09
6.756IleLeu: 6.756 ± 0.678
2.111IleMet: 2.111 ± 0.357
8.867IleAsn: 8.867 ± 1.315
1.604IlePro: 1.604 ± 0.311
2.533IleGln: 2.533 ± 0.421
3.293IleArg: 3.293 ± 0.517
6.418IleSer: 6.418 ± 0.602
5.404IleThr: 5.404 ± 1.06
3.547IleVal: 3.547 ± 0.585
0.76IleTrp: 0.76 ± 0.226
3.124IleTyr: 3.124 ± 0.685
0.0IleXaa: 0.0 ± 0.0
Lys
7.093LysAla: 7.093 ± 1.204
1.436LysCys: 1.436 ± 0.486
6.502LysAsp: 6.502 ± 0.876
11.231LysGlu: 11.231 ± 1.599
4.138LysPhe: 4.138 ± 0.513
5.742LysGly: 5.742 ± 0.752
1.013LysHis: 1.013 ± 0.245
8.191LysIle: 8.191 ± 0.828
10.978LysLys: 10.978 ± 1.68
9.711LysLeu: 9.711 ± 1.162
2.787LysMet: 2.787 ± 0.563
7.685LysAsn: 7.685 ± 1.086
1.773LysPro: 1.773 ± 0.332
3.378LysGln: 3.378 ± 0.512
3.8LysArg: 3.8 ± 0.486
5.32LysSer: 5.32 ± 0.736
5.573LysThr: 5.573 ± 0.719
6.418LysVal: 6.418 ± 0.687
0.844LysTrp: 0.844 ± 0.24
4.56LysTyr: 4.56 ± 0.664
0.0LysXaa: 0.0 ± 0.0
Leu
4.307LeuAla: 4.307 ± 0.724
0.844LeuCys: 0.844 ± 0.297
6.164LeuAsp: 6.164 ± 0.528
7.769LeuGlu: 7.769 ± 0.892
2.196LeuPhe: 2.196 ± 0.483
5.742LeuGly: 5.742 ± 0.919
0.676LeuHis: 0.676 ± 0.205
6.333LeuIle: 6.333 ± 0.862
9.373LeuLys: 9.373 ± 1.213
5.151LeuLeu: 5.151 ± 1.039
1.942LeuMet: 1.942 ± 0.392
6.08LeuAsn: 6.08 ± 0.894
1.858LeuPro: 1.858 ± 0.419
3.124LeuGln: 3.124 ± 0.431
2.956LeuArg: 2.956 ± 0.779
6.418LeuSer: 6.418 ± 0.797
3.716LeuThr: 3.716 ± 0.61
3.293LeuVal: 3.293 ± 0.754
0.591LeuTrp: 0.591 ± 0.231
2.196LeuTyr: 2.196 ± 0.334
0.0LeuXaa: 0.0 ± 0.0
Met
1.351MetAla: 1.351 ± 0.278
0.507MetCys: 0.507 ± 0.263
1.436MetAsp: 1.436 ± 0.528
1.773MetGlu: 1.773 ± 0.383
0.591MetPhe: 0.591 ± 0.232
1.098MetGly: 1.098 ± 0.425
0.338MetHis: 0.338 ± 0.159
1.942MetIle: 1.942 ± 0.339
1.942MetLys: 1.942 ± 0.425
2.28MetLeu: 2.28 ± 0.47
0.169MetMet: 0.169 ± 0.1
1.604MetAsn: 1.604 ± 0.478
0.76MetPro: 0.76 ± 0.282
0.591MetGln: 0.591 ± 0.246
0.507MetArg: 0.507 ± 0.265
1.942MetSer: 1.942 ± 0.469
1.013MetThr: 1.013 ± 0.321
1.013MetVal: 1.013 ± 0.294
0.084MetTrp: 0.084 ± 0.063
0.929MetTyr: 0.929 ± 0.346
0.0MetXaa: 0.0 ± 0.0
Asn
5.32AsnAla: 5.32 ± 0.926
0.929AsnCys: 0.929 ± 0.226
3.293AsnAsp: 3.293 ± 0.654
3.884AsnGlu: 3.884 ± 0.585
3.378AsnPhe: 3.378 ± 0.425
5.151AsnGly: 5.151 ± 0.794
0.422AsnHis: 0.422 ± 0.254
6.925AsnIle: 6.925 ± 0.699
9.036AsnLys: 9.036 ± 0.805
6.502AsnLeu: 6.502 ± 1.177
1.604AsnMet: 1.604 ± 0.371
5.573AsnAsn: 5.573 ± 1.275
1.773AsnPro: 1.773 ± 0.407
1.436AsnGln: 1.436 ± 0.314
3.8AsnArg: 3.8 ± 0.515
6.249AsnSer: 6.249 ± 1.369
4.391AsnThr: 4.391 ± 0.491
3.462AsnVal: 3.462 ± 0.472
0.929AsnTrp: 0.929 ± 0.298
2.196AsnTyr: 2.196 ± 0.369
0.0AsnXaa: 0.0 ± 0.0
Pro
1.182ProAla: 1.182 ± 0.344
0.169ProCys: 0.169 ± 0.1
1.436ProAsp: 1.436 ± 0.349
1.436ProGlu: 1.436 ± 0.331
0.76ProPhe: 0.76 ± 0.234
0.844ProGly: 0.844 ± 0.276
0.338ProHis: 0.338 ± 0.144
1.604ProIle: 1.604 ± 0.301
2.702ProLys: 2.702 ± 0.483
2.618ProLeu: 2.618 ± 0.374
0.929ProMet: 0.929 ± 0.28
1.858ProAsn: 1.858 ± 0.399
0.084ProPro: 0.084 ± 0.068
0.676ProGln: 0.676 ± 0.229
0.253ProArg: 0.253 ± 0.151
1.773ProSer: 1.773 ± 0.317
1.013ProThr: 1.013 ± 0.336
1.351ProVal: 1.351 ± 0.415
0.084ProTrp: 0.084 ± 0.1
0.844ProTyr: 0.844 ± 0.248
0.0ProXaa: 0.0 ± 0.0
Gln
2.533GlnAla: 2.533 ± 0.449
0.338GlnCys: 0.338 ± 0.158
1.351GlnAsp: 1.351 ± 0.327
3.124GlnGlu: 3.124 ± 0.567
1.52GlnPhe: 1.52 ± 0.305
1.351GlnGly: 1.351 ± 0.305
0.169GlnHis: 0.169 ± 0.121
2.787GlnIle: 2.787 ± 0.478
2.871GlnLys: 2.871 ± 0.525
1.773GlnLeu: 1.773 ± 0.304
0.507GlnMet: 0.507 ± 0.264
1.773GlnAsn: 1.773 ± 0.299
0.507GlnPro: 0.507 ± 0.28
1.267GlnGln: 1.267 ± 0.382
1.267GlnArg: 1.267 ± 0.346
2.28GlnSer: 2.28 ± 0.367
1.52GlnThr: 1.52 ± 0.443
1.52GlnVal: 1.52 ± 0.384
0.422GlnTrp: 0.422 ± 0.253
1.267GlnTyr: 1.267 ± 0.304
0.0GlnXaa: 0.0 ± 0.0
Arg
2.533ArgAla: 2.533 ± 0.441
0.169ArgCys: 0.169 ± 0.091
1.858ArgAsp: 1.858 ± 0.41
4.053ArgGlu: 4.053 ± 0.76
1.013ArgPhe: 1.013 ± 0.356
1.942ArgGly: 1.942 ± 0.458
0.422ArgHis: 0.422 ± 0.227
4.138ArgIle: 4.138 ± 0.452
3.547ArgLys: 3.547 ± 0.613
2.618ArgLeu: 2.618 ± 0.539
0.591ArgMet: 0.591 ± 0.2
2.871ArgAsn: 2.871 ± 0.333
0.591ArgPro: 0.591 ± 0.284
1.52ArgGln: 1.52 ± 0.356
1.182ArgArg: 1.182 ± 0.296
1.604ArgSer: 1.604 ± 0.41
1.773ArgThr: 1.773 ± 0.374
2.111ArgVal: 2.111 ± 0.584
0.507ArgTrp: 0.507 ± 0.16
1.52ArgTyr: 1.52 ± 0.37
0.0ArgXaa: 0.0 ± 0.0
Ser
2.449SerAla: 2.449 ± 0.597
0.844SerCys: 0.844 ± 0.295
3.209SerAsp: 3.209 ± 0.631
3.8SerGlu: 3.8 ± 0.48
2.702SerPhe: 2.702 ± 0.496
3.378SerGly: 3.378 ± 0.634
0.844SerHis: 0.844 ± 0.277
6.502SerIle: 6.502 ± 0.606
8.107SerLys: 8.107 ± 0.823
5.32SerLeu: 5.32 ± 0.834
1.52SerMet: 1.52 ± 0.339
4.729SerAsn: 4.729 ± 0.686
1.773SerPro: 1.773 ± 0.383
1.52SerGln: 1.52 ± 0.426
2.28SerArg: 2.28 ± 0.399
4.982SerSer: 4.982 ± 0.664
4.644SerThr: 4.644 ± 0.635
3.631SerVal: 3.631 ± 0.586
0.676SerTrp: 0.676 ± 0.256
2.871SerTyr: 2.871 ± 0.498
0.0SerXaa: 0.0 ± 0.0
Thr
3.04ThrAla: 3.04 ± 0.511
0.507ThrCys: 0.507 ± 0.31
3.378ThrAsp: 3.378 ± 0.667
4.476ThrGlu: 4.476 ± 0.907
2.111ThrPhe: 2.111 ± 0.312
3.631ThrGly: 3.631 ± 0.591
0.422ThrHis: 0.422 ± 0.205
5.067ThrIle: 5.067 ± 0.668
4.729ThrLys: 4.729 ± 0.555
4.813ThrLeu: 4.813 ± 0.514
1.098ThrMet: 1.098 ± 0.298
4.222ThrAsn: 4.222 ± 0.554
2.364ThrPro: 2.364 ± 0.38
2.364ThrGln: 2.364 ± 0.672
1.604ThrArg: 1.604 ± 0.294
3.124ThrSer: 3.124 ± 0.531
4.307ThrThr: 4.307 ± 0.89
3.969ThrVal: 3.969 ± 0.679
0.591ThrTrp: 0.591 ± 0.211
2.618ThrTyr: 2.618 ± 0.458
0.0ThrXaa: 0.0 ± 0.0
Val
3.293ValAla: 3.293 ± 0.729
0.676ValCys: 0.676 ± 0.201
2.618ValAsp: 2.618 ± 0.386
4.053ValGlu: 4.053 ± 0.598
2.28ValPhe: 2.28 ± 0.363
3.124ValGly: 3.124 ± 0.552
0.844ValHis: 0.844 ± 0.28
4.56ValIle: 4.56 ± 0.715
4.644ValLys: 4.644 ± 0.735
4.222ValLeu: 4.222 ± 0.549
0.76ValMet: 0.76 ± 0.261
4.644ValAsn: 4.644 ± 0.691
1.604ValPro: 1.604 ± 0.443
1.351ValGln: 1.351 ± 0.295
1.013ValArg: 1.013 ± 0.296
3.378ValSer: 3.378 ± 0.585
3.716ValThr: 3.716 ± 0.478
1.858ValVal: 1.858 ± 0.466
1.013ValTrp: 1.013 ± 0.302
2.111ValTyr: 2.111 ± 0.499
0.0ValXaa: 0.0 ± 0.0
Trp
0.253TrpAla: 0.253 ± 0.113
0.169TrpCys: 0.169 ± 0.122
0.844TrpAsp: 0.844 ± 0.282
0.844TrpGlu: 0.844 ± 0.329
0.676TrpPhe: 0.676 ± 0.237
0.76TrpGly: 0.76 ± 0.246
0.253TrpHis: 0.253 ± 0.126
1.098TrpIle: 1.098 ± 0.276
0.929TrpLys: 0.929 ± 0.261
0.929TrpLeu: 0.929 ± 0.22
0.253TrpMet: 0.253 ± 0.119
0.507TrpAsn: 0.507 ± 0.209
0.0TrpPro: 0.0 ± 0.0
0.676TrpGln: 0.676 ± 0.276
0.338TrpArg: 0.338 ± 0.149
0.844TrpSer: 0.844 ± 0.241
1.182TrpThr: 1.182 ± 0.281
0.253TrpVal: 0.253 ± 0.151
0.169TrpTrp: 0.169 ± 0.095
0.422TrpTyr: 0.422 ± 0.19
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.604TyrAla: 1.604 ± 0.322
0.507TyrCys: 0.507 ± 0.19
1.773TyrAsp: 1.773 ± 0.53
2.533TyrGlu: 2.533 ± 0.641
1.52TyrPhe: 1.52 ± 0.347
2.027TyrGly: 2.027 ± 0.386
0.591TyrHis: 0.591 ± 0.297
4.053TyrIle: 4.053 ± 0.487
3.884TyrLys: 3.884 ± 0.797
3.124TyrLeu: 3.124 ± 0.614
0.676TyrMet: 0.676 ± 0.276
2.956TyrAsn: 2.956 ± 0.431
1.267TyrPro: 1.267 ± 0.354
1.013TyrGln: 1.013 ± 0.25
2.364TyrArg: 2.364 ± 0.513
2.787TyrSer: 2.787 ± 0.668
1.942TyrThr: 1.942 ± 0.538
2.702TyrVal: 2.702 ± 0.464
0.338TyrTrp: 0.338 ± 0.149
1.436TyrTyr: 1.436 ± 0.411
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (11843 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski