Clostridium sp. CAG:122

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2543 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5RLL5|R5RLL5_9CLOT Stage 0 sporulation protein A homolog OS=Clostridium sp. CAG:122 OX=1262773 GN=BN479_02028 PE=4 SV=1
MM1 pKa = 8.1RR2 pKa = 11.84KK3 pKa = 9.39RR4 pKa = 11.84FLCIICCSMLAFSGCGVGSSVVNNRR29 pKa = 11.84SYY31 pKa = 10.22NTQSEE36 pKa = 4.62SGSSYY41 pKa = 11.56AEE43 pKa = 4.07DD44 pKa = 4.78VEE46 pKa = 4.82DD47 pKa = 4.23TDD49 pKa = 6.2DD50 pKa = 3.84MEE52 pKa = 4.58TDD54 pKa = 2.87EE55 pKa = 6.17DD56 pKa = 5.39YY57 pKa = 11.59YY58 pKa = 11.53DD59 pKa = 4.79EE60 pKa = 5.99DD61 pKa = 3.75SQIYY65 pKa = 9.73DD66 pKa = 3.48VQSKK70 pKa = 8.25YY71 pKa = 11.15LKK73 pKa = 10.49AIQDD77 pKa = 3.64YY78 pKa = 11.26SGTEE82 pKa = 4.09LNDD85 pKa = 3.27LTPDD89 pKa = 3.35NNIEE93 pKa = 4.05NFEE96 pKa = 4.14YY97 pKa = 10.54PEE99 pKa = 5.25DD100 pKa = 3.92EE101 pKa = 5.27SSMQKK106 pKa = 10.27NLNEE110 pKa = 4.09LKK112 pKa = 10.66KK113 pKa = 10.3ILQEE117 pKa = 4.18VYY119 pKa = 10.22GSNVEE124 pKa = 3.91ISFEE128 pKa = 3.9QSSFEE133 pKa = 3.99EE134 pKa = 4.09FDD136 pKa = 3.65VQKK139 pKa = 11.09LNNALSEE146 pKa = 4.88DD147 pKa = 4.01DD148 pKa = 5.76DD149 pKa = 4.85EE150 pKa = 7.69DD151 pKa = 5.68GGDD154 pKa = 3.29VDD156 pKa = 5.81TIEE159 pKa = 4.44TLNKK163 pKa = 8.53TMKK166 pKa = 10.58DD167 pKa = 3.59VVEE170 pKa = 4.74TGCNLQRR177 pKa = 11.84CIKK180 pKa = 10.5SDD182 pKa = 3.11MTFRR186 pKa = 11.84MSGDD190 pKa = 3.33KK191 pKa = 11.41GEE193 pKa = 4.41DD194 pKa = 3.45TQTVASYY201 pKa = 9.97IYY203 pKa = 10.1EE204 pKa = 4.14VDD206 pKa = 3.0GEE208 pKa = 4.85YY209 pKa = 11.6YY210 pKa = 9.48MDD212 pKa = 3.82TYY214 pKa = 11.69AFDD217 pKa = 5.3KK218 pKa = 10.84LLQGQKK224 pKa = 9.03MALEE228 pKa = 4.84EE229 pKa = 4.18KK230 pKa = 10.6QSGNSDD236 pKa = 3.53YY237 pKa = 11.07STADD241 pKa = 3.61LNLDD245 pKa = 3.51QYY247 pKa = 11.64DD248 pKa = 4.26GEE250 pKa = 4.41DD251 pKa = 3.51LNYY254 pKa = 10.83SSLLLDD260 pKa = 3.56QRR262 pKa = 4.33

Molecular weight: 29.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5RPU1|R5RPU1_9CLOT Uncharacterized protein conserved in bacteria OS=Clostridium sp. CAG:122 OX=1262773 GN=BN479_02532 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.33VHH16 pKa = 6.23GFRR19 pKa = 11.84KK20 pKa = 10.11RR21 pKa = 11.84MQTANGRR28 pKa = 11.84KK29 pKa = 8.71VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.26GRR39 pKa = 11.84HH40 pKa = 5.5KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2543

0

2543

847896

30

1818

333.4

37.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.678 ± 0.049

1.426 ± 0.019

6.256 ± 0.038

7.179 ± 0.052

4.106 ± 0.036

6.585 ± 0.04

1.505 ± 0.018

8.185 ± 0.047

8.901 ± 0.058

7.909 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.081 ± 0.029

5.327 ± 0.033

2.721 ± 0.024

2.659 ± 0.027

3.67 ± 0.037

6.331 ± 0.04

5.408 ± 0.049

6.882 ± 0.041

0.764 ± 0.014

4.421 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski