Amino acid dipepetide frequency for Clostridium sp. CAG:122

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.898AlaAla: 5.898 ± 0.115	0.83AlaCys: 0.83 ± 0.033	4.616AlaAsp: 4.616 ± 0.086	4.351AlaGlu: 4.351 ± 0.087	2.636AlaPhe: 2.636 ± 0.067	5.343AlaGly: 5.343 ± 0.088	0.887AlaHis: 0.887 ± 0.036	4.894AlaIle: 4.894 ± 0.086	5.913AlaLys: 5.913 ± 0.106	5.491AlaLeu: 5.491 ± 0.092	2.105AlaMet: 2.105 ± 0.052	2.72AlaAsn: 2.72 ± 0.068	1.532AlaPro: 1.532 ± 0.054	1.764AlaGln: 1.764 ± 0.047	2.146AlaArg: 2.146 ± 0.06	3.896AlaSer: 3.896 ± 0.076	3.117AlaThr: 3.117 ± 0.084	5.672AlaVal: 5.672 ± 0.097	0.478AlaTrp: 0.478 ± 0.025	2.486AlaTyr: 2.486 ± 0.05	0.008AlaXaa: 0.008 ± 0.003
Cys	0.903CysAla: 0.903 ± 0.035	0.282CysCys: 0.282 ± 0.021	0.925CysAsp: 0.925 ± 0.032	0.888CysGlu: 0.888 ± 0.033	0.639CysPhe: 0.639 ± 0.027	1.334CysGly: 1.334 ± 0.044	0.283CysHis: 0.283 ± 0.018	1.23CysIle: 1.23 ± 0.042	0.984CysLys: 0.984 ± 0.034	1.018CysLeu: 1.018 ± 0.037	0.466CysMet: 0.466 ± 0.023	0.728CysAsn: 0.728 ± 0.027	0.472CysPro: 0.472 ± 0.024	0.331CysGln: 0.331 ± 0.017	0.543CysArg: 0.543 ± 0.028	0.869CysSer: 0.869 ± 0.033	0.727CysThr: 0.727 ± 0.028	0.995CysVal: 0.995 ± 0.037	0.085CysTrp: 0.085 ± 0.011	0.563CysTyr: 0.563 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	4.146AspAla: 4.146 ± 0.081	0.844AspCys: 0.844 ± 0.034	4.213AspAsp: 4.213 ± 0.078	5.683AspGlu: 5.683 ± 0.093	2.766AspPhe: 2.766 ± 0.053	4.794AspGly: 4.794 ± 0.089	0.584AspHis: 0.584 ± 0.03	6.152AspIle: 6.152 ± 0.098	5.543AspLys: 5.543 ± 0.093	3.486AspLeu: 3.486 ± 0.069	2.27AspMet: 2.27 ± 0.05	3.331AspAsn: 3.331 ± 0.071	1.13AspPro: 1.13 ± 0.039	0.789AspGln: 0.789 ± 0.028	2.178AspArg: 2.178 ± 0.055	3.526AspSer: 3.526 ± 0.066	3.427AspThr: 3.427 ± 0.07	4.124AspVal: 4.124 ± 0.076	0.471AspTrp: 0.471 ± 0.026	3.097AspTyr: 3.097 ± 0.065	0.002AspXaa: 0.002 ± 0.002
Glu	4.228GluAla: 4.228 ± 0.085	0.896GluCys: 0.896 ± 0.037	4.021GluAsp: 4.021 ± 0.077	5.653GluGlu: 5.653 ± 0.121	2.715GluPhe: 2.715 ± 0.059	3.367GluGly: 3.367 ± 0.061	1.212GluHis: 1.212 ± 0.039	5.886GluIle: 5.886 ± 0.093	7.933GluLys: 7.933 ± 0.129	6.309GluLeu: 6.309 ± 0.107	2.29GluMet: 2.29 ± 0.061	5.102GluAsn: 5.102 ± 0.093	1.645GluPro: 1.645 ± 0.05	2.522GluGln: 2.522 ± 0.063	2.718GluArg: 2.718 ± 0.061	3.641GluSer: 3.641 ± 0.078	3.48GluThr: 3.48 ± 0.062	4.26GluVal: 4.26 ± 0.078	0.541GluTrp: 0.541 ± 0.027	3.371GluTyr: 3.371 ± 0.074	0.0GluXaa: 0.0 ± 0.0
Phe	2.818PheAla: 2.818 ± 0.074	0.732PheCys: 0.732 ± 0.028	2.786PheAsp: 2.786 ± 0.065	2.907PheGlu: 2.907 ± 0.068	1.708PhePhe: 1.708 ± 0.051	2.893PheGly: 2.893 ± 0.063	0.563PheHis: 0.563 ± 0.029	3.348PheIle: 3.348 ± 0.073	2.895PheLys: 2.895 ± 0.061	3.314PheLeu: 3.314 ± 0.082	1.386PheMet: 1.386 ± 0.042	1.921PheAsn: 1.921 ± 0.046	1.076PhePro: 1.076 ± 0.04	0.873PheGln: 0.873 ± 0.034	1.454PheArg: 1.454 ± 0.051	2.987PheSer: 2.987 ± 0.058	2.233PheThr: 2.233 ± 0.05	3.002PheVal: 3.002 ± 0.071	0.354PheTrp: 0.354 ± 0.022	1.81PheTyr: 1.81 ± 0.055	0.001PheXaa: 0.001 ± 0.001
Gly	4.091GlyAla: 4.091 ± 0.086	1.133GlyCys: 1.133 ± 0.045	3.629GlyAsp: 3.629 ± 0.07	4.304GlyGlu: 4.304 ± 0.079	2.987GlyPhe: 2.987 ± 0.062	4.138GlyGly: 4.138 ± 0.069	1.056GlyHis: 1.056 ± 0.037	5.974GlyIle: 5.974 ± 0.101	6.347GlyLys: 6.347 ± 0.098	4.976GlyLeu: 4.976 ± 0.079	2.246GlyMet: 2.246 ± 0.052	3.296GlyAsn: 3.296 ± 0.075	0.962GlyPro: 0.962 ± 0.033	1.745GlyGln: 1.745 ± 0.049	2.639GlyArg: 2.639 ± 0.061	3.805GlySer: 3.805 ± 0.081	3.911GlyThr: 3.911 ± 0.069	4.715GlyVal: 4.715 ± 0.085	0.548GlyTrp: 0.548 ± 0.027	3.345GlyTyr: 3.345 ± 0.072	0.006GlyXaa: 0.006 ± 0.003
His	0.782HisAla: 0.782 ± 0.028	0.246HisCys: 0.246 ± 0.019	0.898HisAsp: 0.898 ± 0.035	0.997HisGlu: 0.997 ± 0.039	0.722HisPhe: 0.722 ± 0.032	1.159HisGly: 1.159 ± 0.037	0.361HisHis: 0.361 ± 0.032	1.422HisIle: 1.422 ± 0.044	1.071HisLys: 1.071 ± 0.038	1.15HisLeu: 1.15 ± 0.037	0.502HisMet: 0.502 ± 0.024	0.756HisAsn: 0.756 ± 0.032	0.665HisPro: 0.665 ± 0.032	0.37HisGln: 0.37 ± 0.021	0.622HisArg: 0.622 ± 0.028	0.919HisSer: 0.919 ± 0.033	0.788HisThr: 0.788 ± 0.031	0.797HisVal: 0.797 ± 0.031	0.121HisTrp: 0.121 ± 0.014	0.699HisTyr: 0.699 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.692IleAla: 5.692 ± 0.087	1.396IleCys: 1.396 ± 0.035	4.979IleAsp: 4.979 ± 0.087	5.64IleGlu: 5.64 ± 0.103	3.339IlePhe: 3.339 ± 0.083	5.113IleGly: 5.113 ± 0.092	1.249IleHis: 1.249 ± 0.039	6.504IleIle: 6.504 ± 0.119	6.717IleLys: 6.717 ± 0.094	6.785IleLeu: 6.785 ± 0.117	2.293IleMet: 2.293 ± 0.056	4.167IleAsn: 4.167 ± 0.077	2.844IlePro: 2.844 ± 0.064	1.929IleGln: 1.929 ± 0.044	3.059IleArg: 3.059 ± 0.054	5.905IleSer: 5.905 ± 0.095	4.649IleThr: 4.649 ± 0.088	5.583IleVal: 5.583 ± 0.097	0.62IleTrp: 0.62 ± 0.027	3.382IleTyr: 3.382 ± 0.072	0.0IleXaa: 0.0 ± 0.0
Lys	5.713LysAla: 5.713 ± 0.107	1.019LysCys: 1.019 ± 0.034	5.312LysAsp: 5.312 ± 0.079	7.1LysGlu: 7.1 ± 0.103	2.803LysPhe: 2.803 ± 0.061	4.527LysGly: 4.527 ± 0.079	1.275LysHis: 1.275 ± 0.045	7.125LysIle: 7.125 ± 0.094	10.063LysLys: 10.063 ± 0.15	6.726LysLeu: 6.726 ± 0.1	2.789LysMet: 2.789 ± 0.056	5.928LysAsn: 5.928 ± 0.088	2.172LysPro: 2.172 ± 0.055	2.714LysGln: 2.714 ± 0.062	3.285LysArg: 3.285 ± 0.071	4.966LysSer: 4.966 ± 0.098	4.915LysThr: 4.915 ± 0.088	5.601LysVal: 5.601 ± 0.099	0.678LysTrp: 0.678 ± 0.03	4.295LysTyr: 4.295 ± 0.068	0.0LysXaa: 0.0 ± 0.0
Leu	5.207LeuAla: 5.207 ± 0.074	1.245LeuCys: 1.245 ± 0.04	4.792LeuAsp: 4.792 ± 0.088	5.459LeuGlu: 5.459 ± 0.095	3.535LeuPhe: 3.535 ± 0.077	4.963LeuGly: 4.963 ± 0.085	1.286LeuHis: 1.286 ± 0.039	5.719LeuIle: 5.719 ± 0.109	7.177LeuLys: 7.177 ± 0.087	6.686LeuLeu: 6.686 ± 0.112	2.257LeuMet: 2.257 ± 0.059	4.097LeuAsn: 4.097 ± 0.071	2.647LeuPro: 2.647 ± 0.053	2.052LeuGln: 2.052 ± 0.048	2.963LeuArg: 2.963 ± 0.059	6.134LeuSer: 6.134 ± 0.095	4.266LeuThr: 4.266 ± 0.074	4.854LeuVal: 4.854 ± 0.089	0.555LeuTrp: 0.555 ± 0.028	3.197LeuTyr: 3.197 ± 0.062	0.001LeuXaa: 0.001 ± 0.001
Met	2.221MetAla: 2.221 ± 0.046	0.441MetCys: 0.441 ± 0.023	1.866MetAsp: 1.866 ± 0.045	2.156MetGlu: 2.156 ± 0.053	1.247MetPhe: 1.247 ± 0.044	1.908MetGly: 1.908 ± 0.051	0.439MetHis: 0.439 ± 0.022	2.224MetIle: 2.224 ± 0.059	2.483MetLys: 2.483 ± 0.051	2.715MetLeu: 2.715 ± 0.063	0.947MetMet: 0.947 ± 0.037	1.695MetAsn: 1.695 ± 0.047	1.17MetPro: 1.17 ± 0.037	1.048MetGln: 1.048 ± 0.037	1.205MetArg: 1.205 ± 0.034	2.077MetSer: 2.077 ± 0.048	1.815MetThr: 1.815 ± 0.045	1.828MetVal: 1.828 ± 0.048	0.217MetTrp: 0.217 ± 0.016	1.106MetTyr: 1.106 ± 0.036	0.0MetXaa: 0.0 ± 0.0
Asn	3.365AsnAla: 3.365 ± 0.069	0.677AsnCys: 0.677 ± 0.03	3.19AsnAsp: 3.19 ± 0.066	3.59AsnGlu: 3.59 ± 0.064	1.829AsnPhe: 1.829 ± 0.047	3.997AsnGly: 3.997 ± 0.082	0.763AsnHis: 0.763 ± 0.028	5.168AsnIle: 5.168 ± 0.078	4.389AsnLys: 4.389 ± 0.078	3.937AsnLeu: 3.937 ± 0.071	1.648AsnMet: 1.648 ± 0.051	2.953AsnAsn: 2.953 ± 0.076	1.917AsnPro: 1.917 ± 0.056	1.304AsnGln: 1.304 ± 0.044	2.034AsnArg: 2.034 ± 0.067	3.291AsnSer: 3.291 ± 0.072	2.947AsnThr: 2.947 ± 0.067	3.668AsnVal: 3.668 ± 0.07	0.355AsnTrp: 0.355 ± 0.022	2.247AsnTyr: 2.247 ± 0.051	0.0AsnXaa: 0.0 ± 0.0
Pro	1.894ProAla: 1.894 ± 0.052	0.337ProCys: 0.337 ± 0.02	2.088ProAsp: 2.088 ± 0.05	2.422ProGlu: 2.422 ± 0.061	1.264ProPhe: 1.264 ± 0.041	1.843ProGly: 1.843 ± 0.048	0.455ProHis: 0.455 ± 0.025	1.95ProIle: 1.95 ± 0.049	2.058ProLys: 2.058 ± 0.055	2.056ProLeu: 2.056 ± 0.053	0.731ProMet: 0.731 ± 0.03	1.169ProAsn: 1.169 ± 0.038	0.498ProPro: 0.498 ± 0.026	0.777ProGln: 0.777 ± 0.034	0.724ProArg: 0.724 ± 0.032	1.55ProSer: 1.55 ± 0.053	1.455ProThr: 1.455 ± 0.072	2.453ProVal: 2.453 ± 0.067	0.235ProTrp: 0.235 ± 0.017	1.248ProTyr: 1.248 ± 0.038	0.005ProXaa: 0.005 ± 0.003
Gln	1.878GlnAla: 1.878 ± 0.066	0.298GlnCys: 0.298 ± 0.018	1.299GlnAsp: 1.299 ± 0.045	1.722GlnGlu: 1.722 ± 0.052	0.998GlnPhe: 0.998 ± 0.034	1.477GlnGly: 1.477 ± 0.043	0.375GlnHis: 0.375 ± 0.019	2.379GlnIle: 2.379 ± 0.057	2.604GlnLys: 2.604 ± 0.069	2.192GlnLeu: 2.192 ± 0.047	0.979GlnMet: 0.979 ± 0.034	1.606GlnAsn: 1.606 ± 0.048	0.708GlnPro: 0.708 ± 0.033	0.9GlnGln: 0.9 ± 0.056	1.034GlnArg: 1.034 ± 0.041	1.636GlnSer: 1.636 ± 0.051	1.49GlnThr: 1.49 ± 0.038	1.57GlnVal: 1.57 ± 0.048	0.237GlnTrp: 0.237 ± 0.02	1.208GlnTyr: 1.208 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	2.079ArgAla: 2.079 ± 0.056	0.494ArgCys: 0.494 ± 0.022	2.013ArgAsp: 2.013 ± 0.057	2.996ArgGlu: 2.996 ± 0.06	1.674ArgPhe: 1.674 ± 0.045	2.056ArgGly: 2.056 ± 0.056	0.629ArgHis: 0.629 ± 0.029	3.127ArgIle: 3.127 ± 0.062	3.526ArgLys: 3.526 ± 0.071	3.122ArgLeu: 3.122 ± 0.068	1.256ArgMet: 1.256 ± 0.038	2.086ArgAsn: 2.086 ± 0.054	0.952ArgPro: 0.952 ± 0.04	1.326ArgGln: 1.326 ± 0.045	1.552ArgArg: 1.552 ± 0.048	1.702ArgSer: 1.702 ± 0.046	1.83ArgThr: 1.83 ± 0.048	2.248ArgVal: 2.248 ± 0.066	0.29ArgTrp: 0.29 ± 0.02	1.743ArgTyr: 1.743 ± 0.046	0.004ArgXaa: 0.004 ± 0.002
Ser	4.136SerAla: 4.136 ± 0.077	0.787SerCys: 0.787 ± 0.027	4.639SerAsp: 4.639 ± 0.089	4.629SerGlu: 4.629 ± 0.085	2.722SerPhe: 2.722 ± 0.055	5.062SerGly: 5.062 ± 0.083	0.992SerHis: 0.992 ± 0.034	4.747SerIle: 4.747 ± 0.091	4.927SerLys: 4.927 ± 0.091	5.012SerLeu: 5.012 ± 0.083	1.815SerMet: 1.815 ± 0.045	2.94SerAsn: 2.94 ± 0.064	1.465SerPro: 1.465 ± 0.049	1.708SerGln: 1.708 ± 0.051	2.308SerArg: 2.308 ± 0.058	4.427SerSer: 4.427 ± 0.114	2.991SerThr: 2.991 ± 0.065	4.846SerVal: 4.846 ± 0.079	0.467SerTrp: 0.467 ± 0.028	2.694SerTyr: 2.694 ± 0.063	0.0SerXaa: 0.0 ± 0.0
Thr	4.226ThrAla: 4.226 ± 0.091	0.571ThrCys: 0.571 ± 0.03	3.71ThrAsp: 3.71 ± 0.079	3.465ThrGlu: 3.465 ± 0.069	2.066ThrPhe: 2.066 ± 0.046	4.385ThrGly: 4.385 ± 0.078	0.771ThrHis: 0.771 ± 0.027	4.156ThrIle: 4.156 ± 0.082	4.184ThrLys: 4.184 ± 0.083	4.218ThrLeu: 4.218 ± 0.064	1.334ThrMet: 1.334 ± 0.04	2.533ThrAsn: 2.533 ± 0.057	1.953ThrPro: 1.953 ± 0.073	1.352ThrGln: 1.352 ± 0.046	1.603ThrArg: 1.603 ± 0.047	3.367ThrSer: 3.367 ± 0.076	2.976ThrThr: 2.976 ± 0.084	4.504ThrVal: 4.504 ± 0.087	0.415ThrTrp: 0.415 ± 0.025	2.283ThrTyr: 2.283 ± 0.059	0.002ThrXaa: 0.002 ± 0.002
Val	4.49ValAla: 4.49 ± 0.089	1.189ValCys: 1.189 ± 0.045	3.921ValAsp: 3.921 ± 0.079	4.39ValGlu: 4.39 ± 0.081	3.044ValPhe: 3.044 ± 0.08	4.042ValGly: 4.042 ± 0.076	0.94ValHis: 0.94 ± 0.039	5.586ValIle: 5.586 ± 0.088	6.028ValLys: 6.028 ± 0.102	5.816ValLeu: 5.816 ± 0.087	2.004ValMet: 2.004 ± 0.05	3.37ValAsn: 3.37 ± 0.048	2.005ValPro: 2.005 ± 0.051	1.573ValGln: 1.573 ± 0.044	2.504ValArg: 2.504 ± 0.062	5.303ValSer: 5.303 ± 0.079	4.251ValThr: 4.251 ± 0.087	4.95ValVal: 4.95 ± 0.097	0.557ValTrp: 0.557 ± 0.031	2.855ValTyr: 2.855 ± 0.058	0.0ValXaa: 0.0 ± 0.0
Trp	0.39TrpAla: 0.39 ± 0.024	0.145TrpCys: 0.145 ± 0.015	0.479TrpAsp: 0.479 ± 0.027	0.476TrpGlu: 0.476 ± 0.029	0.388TrpPhe: 0.388 ± 0.022	0.534TrpGly: 0.534 ± 0.027	0.167TrpHis: 0.167 ± 0.014	0.56TrpIle: 0.56 ± 0.029	0.683TrpLys: 0.683 ± 0.031	0.682TrpLeu: 0.682 ± 0.03	0.224TrpMet: 0.224 ± 0.016	0.455TrpAsn: 0.455 ± 0.024	0.138TrpPro: 0.138 ± 0.012	0.302TrpGln: 0.302 ± 0.02	0.268TrpArg: 0.268 ± 0.02	0.487TrpSer: 0.487 ± 0.026	0.379TrpThr: 0.379 ± 0.022	0.412TrpVal: 0.412 ± 0.022	0.091TrpTrp: 0.091 ± 0.01	0.375TrpTyr: 0.375 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.627TyrAla: 2.627 ± 0.053	0.695TyrCys: 0.695 ± 0.029	3.183TyrAsp: 3.183 ± 0.064	2.964TyrGlu: 2.964 ± 0.063	1.979TyrPhe: 1.979 ± 0.053	2.919TyrGly: 2.919 ± 0.062	0.762TyrHis: 0.762 ± 0.034	3.691TyrIle: 3.691 ± 0.063	3.479TyrLys: 3.479 ± 0.076	3.399TyrLeu: 3.399 ± 0.067	1.263TyrMet: 1.263 ± 0.042	2.414TyrAsn: 2.414 ± 0.056	1.263TyrPro: 1.263 ± 0.042	1.204TyrGln: 1.204 ± 0.04	1.862TyrArg: 1.862 ± 0.052	2.825TyrSer: 2.825 ± 0.064	2.428TyrThr: 2.428 ± 0.065	2.729TyrVal: 2.729 ± 0.052	0.32TyrTrp: 0.32 ± 0.02	2.207TyrTyr: 2.207 ± 0.06	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.004XaaCys: 0.004 ± 0.002	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.004XaaIle: 0.004 ± 0.002	0.001XaaLys: 0.001 ± 0.001	0.004XaaLeu: 0.004 ± 0.002	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.004XaaGln: 0.004 ± 0.002	0.001XaaArg: 0.001 ± 0.001	0.002XaaSer: 0.002 ± 0.002	0.001XaaThr: 0.001 ± 0.001	0.004XaaVal: 0.004 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.034XaaXaa: 0.034 ± 0.008

Statistics based on 2543 proteins (847896 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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