Actinobacteria bacterium OK074
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9137 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N1FUN8|A0A0N1FUN8_9ACTN Peptidase M20 OS=Actinobacteria bacterium OK074 OX=1592327 GN=OK074_4172 PE=4 SV=1
MM1 pKa = 7.66 PAHH4 pKa = 5.64 PTGRR8 pKa = 11.84 RR9 pKa = 11.84 AALLMSALTLTLGTVLAAPIALAGTAVAATPEE41 pKa = 3.82 ATAALGNGGSQLFYY55 pKa = 10.39 TAAAGQANKK64 pKa = 8.45 VTVTVSPGGDD74 pKa = 3.14 TDD76 pKa = 4.17 LSYY79 pKa = 11.43 VIDD82 pKa = 4.34 DD83 pKa = 4.2 VVPIAAGDD91 pKa = 4.07 GCTHH95 pKa = 7.52 PDD97 pKa = 3.12 SADD100 pKa = 3.37 ATKK103 pKa = 10.55 VACTVPEE110 pKa = 5.0 LDD112 pKa = 3.69 SQDD115 pKa = 3.48 PYY117 pKa = 12.08 AVFEE121 pKa = 4.75 ADD123 pKa = 5.17 LGDD126 pKa = 4.2 GNDD129 pKa = 4.67 GITLVDD135 pKa = 3.83 SSDD138 pKa = 3.06 QVYY141 pKa = 10.27 YY142 pKa = 10.84 YY143 pKa = 11.33 NEE145 pKa = 4.19 VNLGTGNDD153 pKa = 3.6 SYY155 pKa = 12.02 VNTGRR160 pKa = 11.84 LDD162 pKa = 3.43 GSLVRR167 pKa = 11.84 GQAGADD173 pKa = 3.54 TITAGEE179 pKa = 4.04 AAIVLAGDD187 pKa = 4.57 DD188 pKa = 4.13 NDD190 pKa = 4.43 SVTANGNYY198 pKa = 10.11 VIVDD202 pKa = 3.83 GGKK205 pKa = 10.81 GNDD208 pKa = 3.78 VIHH211 pKa = 6.83 GGDD214 pKa = 3.44 KK215 pKa = 10.82 GQSLSGGDD223 pKa = 4.32 GNDD226 pKa = 3.12 TLYY229 pKa = 11.07 GGKK232 pKa = 10.68 GNDD235 pKa = 3.13 SLYY238 pKa = 10.84 GGKK241 pKa = 10.74 GNDD244 pKa = 3.57 VLWGNSGNDD253 pKa = 3.62 VLWGNSGNDD262 pKa = 3.45 KK263 pKa = 10.91 LYY265 pKa = 10.94 GGPGTDD271 pKa = 3.66 RR272 pKa = 11.84 LSGGPGTDD280 pKa = 3.06 VTQGG284 pKa = 3.04
Molecular weight: 28.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.745
IPC_protein 3.795
Toseland 3.541
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.795
Rodwell 3.617
Grimsley 3.452
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.253
Thurlkill 3.617
EMBOSS 3.795
Sillero 3.923
Patrickios 1.914
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A0N1G0Z6|A0A0N1G0Z6_9ACTN Alcohol dehydrogenase GroES domain protein OS=Actinobacteria bacterium OK074 OX=1592327 GN=OK074_5661 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILATRR35 pKa = 11.84 RR36 pKa = 11.84 SKK38 pKa = 10.88 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9137
0
9137
3121415
30
7934
341.6
36.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.691 ± 0.041
0.768 ± 0.007
6.013 ± 0.023
5.464 ± 0.028
2.713 ± 0.014
9.531 ± 0.026
2.32 ± 0.013
2.981 ± 0.02
2.029 ± 0.019
10.315 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.619 ± 0.011
1.796 ± 0.013
6.222 ± 0.025
2.732 ± 0.015
7.935 ± 0.03
5.125 ± 0.025
6.532 ± 0.033
8.604 ± 0.025
1.491 ± 0.01
2.12 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here