Rana grylio iridovirus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Iridoviridae; Alphairidovirinae; Ranavirus; Frog virus 3

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 106 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9XFN0|H9XFN0_FRG3V dUTP diphosphatase OS=Rana grylio iridovirus OX=380178 GN=ORF67R PE=3 SV=1
MM1 pKa = 7.31FALEE5 pKa = 4.36TYY7 pKa = 9.33KK8 pKa = 10.87CKK10 pKa = 10.52NSSMSFKK17 pKa = 10.59RR18 pKa = 11.84IMDD21 pKa = 3.65ATDD24 pKa = 2.87IFGDD28 pKa = 3.35ISDD31 pKa = 4.37IGSASSGDD39 pKa = 3.59EE40 pKa = 3.86AEE42 pKa = 4.69FEE44 pKa = 3.91HH45 pKa = 7.49DD46 pKa = 4.07EE47 pKa = 4.19NVEE50 pKa = 3.88RR51 pKa = 11.84AGYY54 pKa = 9.34EE55 pKa = 3.92EE56 pKa = 5.35PDD58 pKa = 3.63DD59 pKa = 5.44DD60 pKa = 5.45GVDD63 pKa = 3.78GVDD66 pKa = 3.83SVNQVEE72 pKa = 4.51AEE74 pKa = 4.06EE75 pKa = 3.88EE76 pKa = 3.84AAAVRR81 pKa = 11.84EE82 pKa = 4.31APSVVMPLDD91 pKa = 3.45MEE93 pKa = 4.76SYY95 pKa = 10.71SDD97 pKa = 3.96EE98 pKa = 4.32EE99 pKa = 4.31LAMMPLGAFASRR111 pKa = 11.84LTQMTAIRR119 pKa = 11.84DD120 pKa = 3.68ALLSDD125 pKa = 4.12FRR127 pKa = 11.84FTGDD131 pKa = 3.7LVTVSDD137 pKa = 3.7KK138 pKa = 10.37YY139 pKa = 11.55LRR141 pKa = 11.84MVAILILNNRR151 pKa = 11.84DD152 pKa = 3.7SLPNPYY158 pKa = 10.1AAYY161 pKa = 10.84NLLQYY166 pKa = 10.65ASKK169 pKa = 10.54RR170 pKa = 11.84GLVNPEE176 pKa = 3.56QAA178 pKa = 3.88

Molecular weight: 19.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9XFL1|H9XFL1_FRG3V Uncharacterized protein OS=Rana grylio iridovirus OX=380178 GN=ORF48L PE=4 SV=1
MM1 pKa = 7.11YY2 pKa = 10.24SVRR5 pKa = 11.84NSGCSAGCSPRR16 pKa = 11.84QGASPIMFGPSLGAMLSAPVVRR38 pKa = 11.84ASSPVVKK45 pKa = 10.32RR46 pKa = 11.84KK47 pKa = 10.35SLVKK51 pKa = 10.26RR52 pKa = 11.84KK53 pKa = 10.2SPVKK57 pKa = 10.34RR58 pKa = 11.84SPLKK62 pKa = 10.44KK63 pKa = 9.82RR64 pKa = 11.84SQTRR68 pKa = 11.84TSPVKK73 pKa = 10.24RR74 pKa = 11.84KK75 pKa = 10.42SPVKK79 pKa = 10.07RR80 pKa = 11.84KK81 pKa = 10.4SPVKK85 pKa = 10.07RR86 pKa = 11.84KK87 pKa = 10.4SPVKK91 pKa = 10.07RR92 pKa = 11.84KK93 pKa = 10.4SPVKK97 pKa = 10.07RR98 pKa = 11.84KK99 pKa = 10.4SPVKK103 pKa = 10.07RR104 pKa = 11.84KK105 pKa = 10.4SPVKK109 pKa = 10.07RR110 pKa = 11.84KK111 pKa = 10.4SPVKK115 pKa = 10.07RR116 pKa = 11.84KK117 pKa = 10.4SPVKK121 pKa = 10.07RR122 pKa = 11.84KK123 pKa = 10.4SPVKK127 pKa = 10.05RR128 pKa = 11.84KK129 pKa = 10.07SPAKK133 pKa = 10.0RR134 pKa = 11.84RR135 pKa = 11.84QQVCFMATKK144 pKa = 10.43

Molecular weight: 16.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

106

0

106

29808

41

1294

281.2

31.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.967 ± 0.355

2.117 ± 0.144

6.059 ± 0.161

5.519 ± 0.224

3.19 ± 0.122

7.458 ± 0.264

2.16 ± 0.146

3.251 ± 0.128

5.566 ± 0.308

8.021 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.056 ± 0.144

2.617 ± 0.124

6.585 ± 0.283

2.835 ± 0.189

7.065 ± 0.308

7.23 ± 0.2

5.512 ± 0.156

8.397 ± 0.225

1.469 ± 0.101

2.925 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski