Pyrobaculum filamentous virus 1

Taxonomy: Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes; Primavirales; Tristromaviridae; Alphatristromavirus; Alphatristromavirus PFV1

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140F3J1|A0A140F3J1_9VIRU Uncharacterized protein OS=Pyrobaculum filamentous virus 1 OX=1805492 PE=4 SV=1
MM1 pKa = 7.57VDD3 pKa = 3.41SQVPMIDD10 pKa = 3.82EE11 pKa = 4.45PLEE14 pKa = 4.36APSVSCEE21 pKa = 3.92EE22 pKa = 3.88LMEE25 pKa = 3.96MCMRR29 pKa = 11.84DD30 pKa = 3.78GVCGVRR36 pKa = 11.84TMKK39 pKa = 10.38HH40 pKa = 4.12YY41 pKa = 10.76VVRR44 pKa = 11.84TYY46 pKa = 11.57DD47 pKa = 3.41KK48 pKa = 11.17GTDD51 pKa = 3.43CC52 pKa = 6.07

Molecular weight: 5.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140F3K1|A0A140F3K1_9VIRU Uncharacterized protein OS=Pyrobaculum filamentous virus 1 OX=1805492 PE=4 SV=1
MM1 pKa = 7.2HH2 pKa = 7.53RR3 pKa = 11.84AEE5 pKa = 4.24KK6 pKa = 10.21FIYY9 pKa = 9.29LWTYY13 pKa = 10.3NSLVPTAIVYY23 pKa = 7.64YY24 pKa = 9.66ASHH27 pKa = 6.65SEE29 pKa = 4.22VVVAMVGVLTTLATWRR45 pKa = 11.84LFTRR49 pKa = 11.84LDD51 pKa = 3.4RR52 pKa = 11.84ALSKK56 pKa = 9.55IAKK59 pKa = 8.77MFGLL63 pKa = 4.18

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39

0

39

5624

40

495

144.2

16.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.579 ± 0.541

1.689 ± 0.294

5.992 ± 0.378

5.743 ± 0.689

3.876 ± 0.295

7.948 ± 0.58

1.245 ± 0.206

6.579 ± 0.253

5.317 ± 0.372

7.29 ± 0.417

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.023 ± 0.356

3.45 ± 0.35

3.076 ± 0.364

1.903 ± 0.233

5.85 ± 0.453

5.957 ± 0.499

5.139 ± 0.595

11.38 ± 0.522

2.098 ± 0.175

5.868 ± 0.583
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski