Amino acid dipepetide frequency for Tuber magnatum (white Piedmont truffle)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.694AlaAla: 7.694 ± 0.055	0.952AlaCys: 0.952 ± 0.019	3.602AlaAsp: 3.602 ± 0.03	5.134AlaGlu: 5.134 ± 0.044	2.775AlaPhe: 2.775 ± 0.029	6.008AlaGly: 6.008 ± 0.05	1.666AlaHis: 1.666 ± 0.022	4.106AlaIle: 4.106 ± 0.034	4.055AlaLys: 4.055 ± 0.042	7.37AlaLeu: 7.37 ± 0.051	1.727AlaMet: 1.727 ± 0.023	2.697AlaAsn: 2.697 ± 0.025	4.721AlaPro: 4.721 ± 0.05	2.773AlaGln: 2.773 ± 0.028	4.843AlaArg: 4.843 ± 0.034	6.425AlaSer: 6.425 ± 0.053	4.832AlaThr: 4.832 ± 0.041	5.239AlaVal: 5.239 ± 0.042	0.948AlaTrp: 0.948 ± 0.017	1.945AlaTyr: 1.945 ± 0.023	0.002AlaXaa: 0.002 ± 0.001
Cys	0.916CysAla: 0.916 ± 0.016	0.269CysCys: 0.269 ± 0.011	0.643CysAsp: 0.643 ± 0.014	0.73CysGlu: 0.73 ± 0.014	0.54CysPhe: 0.54 ± 0.012	1.118CysGly: 1.118 ± 0.022	0.325CysHis: 0.325 ± 0.01	0.669CysIle: 0.669 ± 0.015	0.579CysLys: 0.579 ± 0.014	1.245CysLeu: 1.245 ± 0.021	0.291CysMet: 0.291 ± 0.01	0.42CysAsn: 0.42 ± 0.012	0.703CysPro: 0.703 ± 0.017	0.399CysGln: 0.399 ± 0.013	0.773CysArg: 0.773 ± 0.016	0.945CysSer: 0.945 ± 0.019	0.729CysThr: 0.729 ± 0.018	0.819CysVal: 0.819 ± 0.014	0.203CysTrp: 0.203 ± 0.007	0.377CysTyr: 0.377 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	3.782AspAla: 3.782 ± 0.031	0.64AspCys: 0.64 ± 0.015	3.719AspAsp: 3.719 ± 0.05	4.411AspGlu: 4.411 ± 0.042	2.009AspPhe: 2.009 ± 0.025	4.253AspGly: 4.253 ± 0.041	1.105AspHis: 1.105 ± 0.019	2.99AspIle: 2.99 ± 0.03	2.358AspLys: 2.358 ± 0.029	4.628AspLeu: 4.628 ± 0.04	1.177AspMet: 1.177 ± 0.018	1.797AspAsn: 1.797 ± 0.024	3.154AspPro: 3.154 ± 0.032	1.535AspGln: 1.535 ± 0.02	3.027AspArg: 3.027 ± 0.037	3.89AspSer: 3.89 ± 0.034	2.727AspThr: 2.727 ± 0.026	3.415AspVal: 3.415 ± 0.027	0.8AspTrp: 0.8 ± 0.015	1.521AspTyr: 1.521 ± 0.021	0.001AspXaa: 0.001 ± 0.0
Glu	5.199GluAla: 5.199 ± 0.042	0.738GluCys: 0.738 ± 0.014	4.364GluAsp: 4.364 ± 0.047	6.913GluGlu: 6.913 ± 0.073	2.192GluPhe: 2.192 ± 0.026	5.029GluGly: 5.029 ± 0.044	1.312GluHis: 1.312 ± 0.017	3.441GluIle: 3.441 ± 0.031	4.419GluLys: 4.419 ± 0.044	5.452GluLeu: 5.452 ± 0.043	1.681GluMet: 1.681 ± 0.021	2.479GluAsn: 2.479 ± 0.027	2.578GluPro: 2.578 ± 0.031	2.124GluGln: 2.124 ± 0.025	4.636GluArg: 4.636 ± 0.044	4.269GluSer: 4.269 ± 0.037	3.301GluThr: 3.301 ± 0.033	4.261GluVal: 4.261 ± 0.041	0.959GluTrp: 0.959 ± 0.015	1.802GluTyr: 1.802 ± 0.023	0.001GluXaa: 0.001 ± 0.001
Phe	2.751PheAla: 2.751 ± 0.03	0.57PheCys: 0.57 ± 0.013	2.043PheAsp: 2.043 ± 0.021	2.15PheGlu: 2.15 ± 0.022	1.57PhePhe: 1.57 ± 0.018	2.828PheGly: 2.828 ± 0.034	0.856PheHis: 0.856 ± 0.017	1.694PheIle: 1.694 ± 0.02	1.543PheLys: 1.543 ± 0.021	3.545PheLeu: 3.545 ± 0.035	0.733PheMet: 0.733 ± 0.014	1.311PheAsn: 1.311 ± 0.019	1.997PhePro: 1.997 ± 0.024	1.203PheGln: 1.203 ± 0.018	2.051PheArg: 2.051 ± 0.024	2.972PheSer: 2.972 ± 0.03	2.174PheThr: 2.174 ± 0.022	2.339PheVal: 2.339 ± 0.029	0.577PheTrp: 0.577 ± 0.013	1.069PheTyr: 1.069 ± 0.017	0.002PheXaa: 0.002 ± 0.001
Gly	5.479GlyAla: 5.479 ± 0.04	0.964GlyCys: 0.964 ± 0.019	4.091GlyAsp: 4.091 ± 0.044	4.836GlyGlu: 4.836 ± 0.038	2.842GlyPhe: 2.842 ± 0.033	7.944GlyGly: 7.944 ± 0.076	1.596GlyHis: 1.596 ± 0.026	3.893GlyIle: 3.893 ± 0.033	4.259GlyLys: 4.259 ± 0.038	6.093GlyLeu: 6.093 ± 0.048	1.779GlyMet: 1.779 ± 0.025	2.899GlyAsn: 2.899 ± 0.033	3.347GlyPro: 3.347 ± 0.037	2.305GlyGln: 2.305 ± 0.027	4.91GlyArg: 4.91 ± 0.04	6.205GlySer: 6.205 ± 0.05	4.084GlyThr: 4.084 ± 0.037	5.091GlyVal: 5.091 ± 0.048	1.211GlyTrp: 1.211 ± 0.018	2.206GlyTyr: 2.206 ± 0.029	0.003GlyXaa: 0.003 ± 0.001
His	1.585HisAla: 1.585 ± 0.023	0.323HisCys: 0.323 ± 0.01	1.059HisAsp: 1.059 ± 0.018	1.251HisGlu: 1.251 ± 0.019	0.889HisPhe: 0.889 ± 0.014	1.638HisGly: 1.638 ± 0.026	0.945HisHis: 0.945 ± 0.021	1.16HisIle: 1.16 ± 0.019	0.984HisLys: 0.984 ± 0.016	2.237HisLeu: 2.237 ± 0.025	0.458HisMet: 0.458 ± 0.011	0.82HisAsn: 0.82 ± 0.014	1.79HisPro: 1.79 ± 0.024	0.975HisGln: 0.975 ± 0.019	1.589HisArg: 1.589 ± 0.023	1.898HisSer: 1.898 ± 0.03	1.274HisThr: 1.274 ± 0.02	1.319HisVal: 1.319 ± 0.018	0.319HisTrp: 0.319 ± 0.009	0.687HisTyr: 0.687 ± 0.015	0.001HisXaa: 0.001 ± 0.0
Ile	4.097IleAla: 4.097 ± 0.04	0.767IleCys: 0.767 ± 0.015	2.693IleAsp: 2.693 ± 0.029	3.041IleGlu: 3.041 ± 0.033	1.884IlePhe: 1.884 ± 0.028	3.326IleGly: 3.326 ± 0.029	1.227IleHis: 1.227 ± 0.018	2.485IleIle: 2.485 ± 0.029	2.348IleLys: 2.348 ± 0.025	4.779IleLeu: 4.779 ± 0.041	0.986IleMet: 0.986 ± 0.015	1.773IleAsn: 1.773 ± 0.021	3.255IlePro: 3.255 ± 0.032	1.696IleGln: 1.696 ± 0.021	3.019IleArg: 3.019 ± 0.028	4.08IleSer: 4.08 ± 0.035	2.994IleThr: 2.994 ± 0.025	3.224IleVal: 3.224 ± 0.034	0.68IleTrp: 0.68 ± 0.015	1.392IleTyr: 1.392 ± 0.021	0.001IleXaa: 0.001 ± 0.0
Lys	4.215LysAla: 4.215 ± 0.042	0.611LysCys: 0.611 ± 0.013	2.783LysAsp: 2.783 ± 0.028	4.035LysGlu: 4.035 ± 0.041	1.597LysPhe: 1.597 ± 0.022	3.647LysGly: 3.647 ± 0.036	1.147LysHis: 1.147 ± 0.018	2.593LysIle: 2.593 ± 0.029	3.834LysLys: 3.834 ± 0.05	4.344LysLeu: 4.344 ± 0.037	1.128LysMet: 1.128 ± 0.016	1.897LysAsn: 1.897 ± 0.023	2.802LysPro: 2.802 ± 0.031	1.739LysGln: 1.739 ± 0.023	3.961LysArg: 3.961 ± 0.039	3.647LysSer: 3.647 ± 0.037	2.885LysThr: 2.885 ± 0.026	3.159LysVal: 3.159 ± 0.028	0.725LysTrp: 0.725 ± 0.014	1.426LysTyr: 1.426 ± 0.02	0.002LysXaa: 0.002 ± 0.001
Leu	7.349LeuAla: 7.349 ± 0.051	1.249LeuCys: 1.249 ± 0.021	4.74LeuAsp: 4.74 ± 0.039	6.048LeuGlu: 6.048 ± 0.046	3.288LeuPhe: 3.288 ± 0.033	6.266LeuGly: 6.266 ± 0.052	2.199LeuHis: 2.199 ± 0.024	3.867LeuIle: 3.867 ± 0.035	4.597LeuLys: 4.597 ± 0.037	8.652LeuLeu: 8.652 ± 0.057	1.758LeuMet: 1.758 ± 0.022	3.026LeuAsn: 3.026 ± 0.03	5.515LeuPro: 5.515 ± 0.042	3.457LeuGln: 3.457 ± 0.034	5.853LeuArg: 5.853 ± 0.048	7.224LeuSer: 7.224 ± 0.053	4.752LeuThr: 4.752 ± 0.038	5.55LeuVal: 5.55 ± 0.042	1.153LeuTrp: 1.153 ± 0.023	2.302LeuTyr: 2.302 ± 0.03	0.001LeuXaa: 0.001 ± 0.001
Met	1.93MetAla: 1.93 ± 0.023	0.256MetCys: 0.256 ± 0.007	1.275MetAsp: 1.275 ± 0.016	1.552MetGlu: 1.552 ± 0.021	0.736MetPhe: 0.736 ± 0.014	1.669MetGly: 1.669 ± 0.02	0.451MetHis: 0.451 ± 0.009	1.005MetIle: 1.005 ± 0.016	1.13MetLys: 1.13 ± 0.017	1.777MetLeu: 1.777 ± 0.027	0.524MetMet: 0.524 ± 0.013	0.769MetAsn: 0.769 ± 0.013	1.195MetPro: 1.195 ± 0.019	0.702MetGln: 0.702 ± 0.016	1.295MetArg: 1.295 ± 0.021	1.782MetSer: 1.782 ± 0.021	1.137MetThr: 1.137 ± 0.017	1.383MetVal: 1.383 ± 0.018	0.258MetTrp: 0.258 ± 0.009	0.501MetTyr: 0.501 ± 0.013	0.001MetXaa: 0.001 ± 0.0
Asn	2.759AsnAla: 2.759 ± 0.027	0.478AsnCys: 0.478 ± 0.012	1.733AsnAsp: 1.733 ± 0.023	1.969AsnGlu: 1.969 ± 0.024	1.35AsnPhe: 1.35 ± 0.021	2.892AsnGly: 2.892 ± 0.032	0.865AsnHis: 0.865 ± 0.016	2.028AsnIle: 2.028 ± 0.025	1.655AsnLys: 1.655 ± 0.024	3.296AsnLeu: 3.296 ± 0.034	0.77AsnMet: 0.77 ± 0.014	1.484AsnAsn: 1.484 ± 0.022	2.573AsnPro: 2.573 ± 0.027	1.168AsnGln: 1.168 ± 0.017	2.166AsnArg: 2.166 ± 0.022	2.975AsnSer: 2.975 ± 0.035	2.202AsnThr: 2.202 ± 0.028	2.196AsnVal: 2.196 ± 0.026	0.53AsnTrp: 0.53 ± 0.012	1.032AsnTyr: 1.032 ± 0.018	0.002AsnXaa: 0.002 ± 0.001
Pro	4.946ProAla: 4.946 ± 0.041	0.511ProCys: 0.511 ± 0.012	2.921ProAsp: 2.921 ± 0.033	3.878ProGlu: 3.878 ± 0.038	1.927ProPhe: 1.927 ± 0.025	4.24ProGly: 4.24 ± 0.039	1.47ProHis: 1.47 ± 0.025	2.708ProIle: 2.708 ± 0.031	2.87ProLys: 2.87 ± 0.032	4.964ProLeu: 4.964 ± 0.041	1.037ProMet: 1.037 ± 0.019	2.203ProAsn: 2.203 ± 0.029	6.505ProPro: 6.505 ± 0.099	2.375ProGln: 2.375 ± 0.037	3.512ProArg: 3.512 ± 0.036	5.876ProSer: 5.876 ± 0.057	4.397ProThr: 4.397 ± 0.043	3.581ProVal: 3.581 ± 0.038	0.593ProTrp: 0.593 ± 0.014	1.457ProTyr: 1.457 ± 0.023	0.004ProXaa: 0.004 ± 0.001
Gln	2.777GlnAla: 2.777 ± 0.028	0.408GlnCys: 0.408 ± 0.011	1.667GlnAsp: 1.667 ± 0.023	2.323GlnGlu: 2.323 ± 0.027	1.172GlnPhe: 1.172 ± 0.017	2.221GlnGly: 2.221 ± 0.03	0.921GlnHis: 0.921 ± 0.02	1.693GlnIle: 1.693 ± 0.023	1.937GlnLys: 1.937 ± 0.026	3.015GlnLeu: 3.015 ± 0.032	0.794GlnMet: 0.794 ± 0.014	1.425GlnAsn: 1.425 ± 0.022	2.141GlnPro: 2.141 ± 0.037	2.312GlnGln: 2.312 ± 0.061	2.359GlnArg: 2.359 ± 0.026	2.608GlnSer: 2.608 ± 0.029	2.049GlnThr: 2.049 ± 0.023	2.014GlnVal: 2.014 ± 0.026	0.44GlnTrp: 0.44 ± 0.012	0.997GlnTyr: 0.997 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	4.763ArgAla: 4.763 ± 0.036	0.777ArgCys: 0.777 ± 0.015	3.343ArgAsp: 3.343 ± 0.037	4.623ArgGlu: 4.623 ± 0.042	2.178ArgPhe: 2.178 ± 0.025	4.633ArgGly: 4.633 ± 0.041	1.459ArgHis: 1.459 ± 0.025	3.236ArgIle: 3.236 ± 0.029	4.129ArgLys: 4.129 ± 0.037	5.426ArgLeu: 5.426 ± 0.035	1.505ArgMet: 1.505 ± 0.021	2.419ArgAsn: 2.419 ± 0.026	3.344ArgPro: 3.344 ± 0.034	2.296ArgGln: 2.296 ± 0.025	5.506ArgArg: 5.506 ± 0.048	4.818ArgSer: 4.818 ± 0.047	3.325ArgThr: 3.325 ± 0.032	3.923ArgVal: 3.923 ± 0.033	0.892ArgTrp: 0.892 ± 0.018	1.752ArgTyr: 1.752 ± 0.021	0.003ArgXaa: 0.003 ± 0.001
Ser	6.345SerAla: 6.345 ± 0.05	0.893SerCys: 0.893 ± 0.017	3.819SerAsp: 3.819 ± 0.036	4.266SerGlu: 4.266 ± 0.033	2.817SerPhe: 2.817 ± 0.027	6.265SerGly: 6.265 ± 0.047	1.877SerHis: 1.877 ± 0.025	3.861SerIle: 3.861 ± 0.035	3.625SerLys: 3.625 ± 0.033	7.138SerLeu: 7.138 ± 0.046	1.613SerMet: 1.613 ± 0.021	2.855SerAsn: 2.855 ± 0.032	6.037SerPro: 6.037 ± 0.062	2.797SerGln: 2.797 ± 0.032	5.056SerArg: 5.056 ± 0.05	8.644SerSer: 8.644 ± 0.094	5.457SerThr: 5.457 ± 0.05	4.531SerVal: 4.531 ± 0.038	0.982SerTrp: 0.982 ± 0.018	2.04SerTyr: 2.04 ± 0.024	0.003SerXaa: 0.003 ± 0.001
Thr	4.991ThrAla: 4.991 ± 0.037	0.749ThrCys: 0.749 ± 0.017	2.526ThrAsp: 2.526 ± 0.027	3.052ThrGlu: 3.052 ± 0.03	2.14ThrPhe: 2.14 ± 0.021	4.336ThrGly: 4.336 ± 0.037	1.344ThrHis: 1.344 ± 0.021	3.142ThrIle: 3.142 ± 0.034	2.64ThrLys: 2.64 ± 0.029	5.305ThrLeu: 5.305 ± 0.04	1.068ThrMet: 1.068 ± 0.018	2.02ThrAsn: 2.02 ± 0.025	4.755ThrPro: 4.755 ± 0.046	1.868ThrGln: 1.868 ± 0.022	3.274ThrArg: 3.274 ± 0.03	5.112ThrSer: 5.112 ± 0.042	4.296ThrThr: 4.296 ± 0.052	3.528ThrVal: 3.528 ± 0.032	0.737ThrTrp: 0.737 ± 0.014	1.498ThrTyr: 1.498 ± 0.021	0.002ThrXaa: 0.002 ± 0.001
Val	5.019ValAla: 5.019 ± 0.037	0.887ValCys: 0.887 ± 0.017	3.581ValAsp: 3.581 ± 0.035	4.405ValGlu: 4.405 ± 0.037	2.408ValPhe: 2.408 ± 0.027	4.659ValGly: 4.659 ± 0.044	1.39ValHis: 1.39 ± 0.018	3.095ValIle: 3.095 ± 0.03	3.214ValLys: 3.214 ± 0.03	5.724ValLeu: 5.724 ± 0.047	1.382ValMet: 1.382 ± 0.017	2.222ValAsn: 2.222 ± 0.029	3.527ValPro: 3.527 ± 0.03	2.098ValGln: 2.098 ± 0.026	3.826ValArg: 3.826 ± 0.032	4.516ValSer: 4.516 ± 0.04	3.414ValThr: 3.414 ± 0.032	4.661ValVal: 4.661 ± 0.05	0.873ValTrp: 0.873 ± 0.018	1.785ValTyr: 1.785 ± 0.025	0.001ValXaa: 0.001 ± 0.001
Trp	0.981TrpAla: 0.981 ± 0.02	0.189TrpCys: 0.189 ± 0.008	0.82TrpAsp: 0.82 ± 0.015	0.962TrpGlu: 0.962 ± 0.019	0.486TrpPhe: 0.486 ± 0.011	1.078TrpGly: 1.078 ± 0.02	0.296TrpHis: 0.296 ± 0.009	0.725TrpIle: 0.725 ± 0.013	0.82TrpLys: 0.82 ± 0.016	1.217TrpLeu: 1.217 ± 0.02	0.35TrpMet: 0.35 ± 0.01	0.547TrpAsn: 0.547 ± 0.012	0.499TrpPro: 0.499 ± 0.011	0.427TrpGln: 0.427 ± 0.012	0.958TrpArg: 0.958 ± 0.016	0.925TrpSer: 0.925 ± 0.017	0.722TrpThr: 0.722 ± 0.015	0.909TrpVal: 0.909 ± 0.015	0.271TrpTrp: 0.271 ± 0.01	0.388TrpTyr: 0.388 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.934TyrAla: 1.934 ± 0.024	0.46TyrCys: 0.46 ± 0.012	1.514TyrAsp: 1.514 ± 0.023	1.569TyrGlu: 1.569 ± 0.023	1.176TyrPhe: 1.176 ± 0.02	1.983TyrGly: 1.983 ± 0.024	0.745TyrHis: 0.745 ± 0.014	1.357TyrIle: 1.357 ± 0.02	1.183TyrLys: 1.183 ± 0.02	2.637TyrLeu: 2.637 ± 0.029	0.568TyrMet: 0.568 ± 0.014	1.06TyrAsn: 1.06 ± 0.018	1.535TyrPro: 1.535 ± 0.024	1.022TyrGln: 1.022 ± 0.015	1.731TyrArg: 1.731 ± 0.023	2.107TyrSer: 2.107 ± 0.023	1.629TyrThr: 1.629 ± 0.02	1.546TyrVal: 1.546 ± 0.021	0.42TyrTrp: 0.42 ± 0.011	0.905TyrTyr: 0.905 ± 0.017	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.002XaaPhe: 0.002 ± 0.001	0.005XaaGly: 0.005 ± 0.001	0.001XaaHis: 0.001 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.003XaaLeu: 0.003 ± 0.001	0.002XaaMet: 0.002 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.003XaaArg: 0.003 ± 0.001	0.002XaaSer: 0.002 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.001XaaTrp: 0.001 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9390 proteins (3818676 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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