Bifidobacterium phage PMBT6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345AR44|A0A345AR44_9CAUD Antirepressor protein OS=Bifidobacterium phage PMBT6 OX=2576879 PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 7.85EE3 pKa = 4.24TKK5 pKa = 10.36DD6 pKa = 3.39LTDD9 pKa = 3.45AQLAARR15 pKa = 11.84DD16 pKa = 3.91VTCFVVGMALMLVGAWACVWFVWGVVTLHH45 pKa = 5.99VVGTVEE51 pKa = 4.31GLLMLPCWLFGITLLDD67 pKa = 3.22IAVNSTTDD75 pKa = 3.25GKK77 pKa = 10.88DD78 pKa = 3.04EE79 pKa = 4.09

Molecular weight: 8.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345AR59|A0A345AR59_9CAUD Uncharacterized protein OS=Bifidobacterium phage PMBT6 OX=2576879 PE=4 SV=1
MM1 pKa = 7.52SKK3 pKa = 10.13RR4 pKa = 11.84RR5 pKa = 11.84NEE7 pKa = 3.91RR8 pKa = 11.84VRR10 pKa = 11.84NGYY13 pKa = 8.17RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84MLRR19 pKa = 11.84QRR21 pKa = 11.84VLAAYY26 pKa = 7.64DD27 pKa = 3.31VCAICGKK34 pKa = 9.72PVDD37 pKa = 4.13KK38 pKa = 10.26TLKK41 pKa = 9.06TPHH44 pKa = 6.67PMSAEE49 pKa = 3.83VDD51 pKa = 3.54EE52 pKa = 4.94LVPVSRR58 pKa = 11.84GGNPYY63 pKa = 10.7SFANCRR69 pKa = 11.84LTHH72 pKa = 6.72RR73 pKa = 11.84ICNRR77 pKa = 11.84MKK79 pKa = 10.43SDD81 pKa = 3.19KK82 pKa = 10.13TDD84 pKa = 2.93EE85 pKa = 4.09HH86 pKa = 7.39ARR88 pKa = 11.84ALLAGEE94 pKa = 4.22RR95 pKa = 11.84TIKK98 pKa = 10.46PSSLPFKK105 pKa = 10.57TFGII109 pKa = 4.38

Molecular weight: 12.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

10676

39

920

177.9

19.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.088 ± 0.473

1.133 ± 0.16

7.147 ± 0.367

5.189 ± 0.402

2.295 ± 0.194

8.477 ± 0.499

2.126 ± 0.198

5.405 ± 0.363

4.477 ± 0.292

8.102 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.679 ± 0.173

3.232 ± 0.214

4.534 ± 0.314

3.55 ± 0.272

6.753 ± 0.518

6.341 ± 0.389

7.54 ± 0.449

6.566 ± 0.352

1.986 ± 0.128

2.379 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski