Lactobacillus phage 3-521

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Watanabevirus; Lactobacillus virus 3-521

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 155 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5FGG0|A0A4Y5FGG0_9CAUD Transposase OS=Lactobacillus phage 3-521 OX=2510943 GN=UCC3521_0029 PE=3 SV=1
MM1 pKa = 7.21TSKK4 pKa = 9.39FTAEE8 pKa = 4.06QQEE11 pKa = 4.47KK12 pKa = 10.75AMEE15 pKa = 4.52LLTDD19 pKa = 3.99NGDD22 pKa = 4.0LNIMPIEE29 pKa = 4.18EE30 pKa = 4.39FMTAYY35 pKa = 9.94EE36 pKa = 4.35PYY38 pKa = 9.96EE39 pKa = 3.68IAKK42 pKa = 8.77MVKK45 pKa = 9.8QSNLNLDD52 pKa = 3.51CDD54 pKa = 4.16YY55 pKa = 11.57VRR57 pKa = 11.84VNDD60 pKa = 4.19YY61 pKa = 11.45YY62 pKa = 11.65SDD64 pKa = 3.28THH66 pKa = 6.39EE67 pKa = 4.91ADD69 pKa = 4.04DD70 pKa = 4.43MYY72 pKa = 11.84ALLSDD77 pKa = 4.89DD78 pKa = 4.94EE79 pKa = 4.63IEE81 pKa = 4.22EE82 pKa = 4.28ALQEE86 pKa = 4.16LQQ88 pKa = 3.92

Molecular weight: 10.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5FGI8|A0A4Y5FGI8_9CAUD Homing endonuclease OS=Lactobacillus phage 3-521 OX=2510943 GN=UCC3521_0050 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.24RR3 pKa = 11.84VQKK6 pKa = 9.98PGKK9 pKa = 10.3DD10 pKa = 3.16MGSTLGGILGAVLGFTTRR28 pKa = 11.84LALDD32 pKa = 4.47MISLFYY38 pKa = 10.72VSRR41 pKa = 11.84ALSSIIVGYY50 pKa = 8.14PIRR53 pKa = 11.84YY54 pKa = 8.52QVDD57 pKa = 2.73IRR59 pKa = 11.84EE60 pKa = 4.35TIVAVLYY67 pKa = 10.47IILRR71 pKa = 11.84LLGDD75 pKa = 4.07LVQNRR80 pKa = 11.84LTKK83 pKa = 10.67

Molecular weight: 9.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

155

0

155

41627

37

1857

268.6

30.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.631 ± 0.208

0.646 ± 0.07

7.082 ± 0.155

5.624 ± 0.242

3.209 ± 0.097

6.198 ± 0.24

1.763 ± 0.095

5.696 ± 0.146

7.291 ± 0.251

8.43 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.258 ± 0.095

6.38 ± 0.149

3.258 ± 0.127

3.829 ± 0.118

3.094 ± 0.146

8.845 ± 0.298

7.224 ± 0.307

6.993 ± 0.171

1.023 ± 0.081

5.525 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski