Azoarcus sp. M9-3-2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae; Azoarcus; unclassified Azoarcus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3806 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6C1B5F1|A0A6C1B5F1_9RHOO BolA family transcriptional regulator OS=Azoarcus sp. M9-3-2 OX=2016596 GN=G3580_07600 PE=3 SV=1
MM1 pKa = 7.42MEE3 pKa = 4.38TTQMDD8 pKa = 4.2EE9 pKa = 4.52LLVFTDD15 pKa = 3.84SAANKK20 pKa = 9.04VKK22 pKa = 10.42EE23 pKa = 4.28LIEE26 pKa = 4.17EE27 pKa = 4.16EE28 pKa = 4.5GNAEE32 pKa = 3.71LKK34 pKa = 10.89LRR36 pKa = 11.84VFVTGGGCSGFQYY49 pKa = 10.98GFTFDD54 pKa = 3.92EE55 pKa = 5.44DD56 pKa = 3.76VAEE59 pKa = 5.68DD60 pKa = 3.58DD61 pKa = 4.05TAMEE65 pKa = 4.48KK66 pKa = 10.89NGVTLLVDD74 pKa = 3.69PMSYY78 pKa = 10.34QYY80 pKa = 11.57LVGAEE85 pKa = 3.64IDD87 pKa = 3.75YY88 pKa = 10.81TEE90 pKa = 4.44GLEE93 pKa = 4.19GAQFVIRR100 pKa = 11.84NPNATTTCGCGSSFSVV116 pKa = 3.54

Molecular weight: 12.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6C1B897|A0A6C1B897_9RHOO Uncharacterized protein OS=Azoarcus sp. M9-3-2 OX=2016596 GN=G3580_03705 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.12QPSVVKK11 pKa = 10.65RR12 pKa = 11.84KK13 pKa = 8.06RR14 pKa = 11.84THH16 pKa = 5.75GFLVRR21 pKa = 11.84MRR23 pKa = 11.84SRR25 pKa = 11.84GGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84HH40 pKa = 4.92RR41 pKa = 11.84LAVV44 pKa = 3.37

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3806

0

3806

1265655

24

5284

332.5

36.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.781 ± 0.054

1.012 ± 0.018

5.964 ± 0.034

5.588 ± 0.041

3.568 ± 0.026

8.364 ± 0.05

2.478 ± 0.023

4.6 ± 0.031

2.823 ± 0.033

10.543 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.433 ± 0.022

2.439 ± 0.028

5.194 ± 0.035

3.329 ± 0.026

7.518 ± 0.045

4.855 ± 0.034

5.211 ± 0.043

7.675 ± 0.035

1.4 ± 0.018

2.225 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski