Acetobacter sp. CAG:267
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1538 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R5YN98|R5YN98_9PROT Predicted permease OS=Acetobacter sp. CAG:267 OX=1262684 GN=BN575_00604 PE=4 SV=1
MM1 pKa = 7.43 LTTLLIYY8 pKa = 10.86 YY9 pKa = 7.51 GGCALGLFGMAKK21 pKa = 9.71 VVEE24 pKa = 4.74 HH25 pKa = 6.56 LPEE28 pKa = 6.0 SDD30 pKa = 3.49 GKK32 pKa = 11.42 SSGTDD37 pKa = 3.21 DD38 pKa = 4.44 YY39 pKa = 11.93 GSSSYY44 pKa = 11.39 DD45 pKa = 3.39 DD46 pKa = 4.15 NLGFDD51 pKa = 4.47 GEE53 pKa = 4.73 PNGMPYY59 pKa = 9.4 MPP61 pKa = 5.58
Molecular weight: 6.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.873
IPC2_protein 3.872
IPC_protein 3.745
Toseland 3.554
ProMoST 3.961
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.681
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.884
Patrickios 0.121
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.808
Protein with the highest isoelectric point:
>tr|R5Y1K1|R5Y1K1_9PROT Uncharacterized protein OS=Acetobacter sp. CAG:267 OX=1262684 GN=BN575_01252 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.43 LVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.36 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MATAGGRR28 pKa = 11.84 KK29 pKa = 8.88 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1538
0
1538
481817
29
2195
313.3
34.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.111 ± 0.079
1.287 ± 0.025
5.541 ± 0.054
6.775 ± 0.067
4.588 ± 0.053
7.001 ± 0.087
1.568 ± 0.023
6.536 ± 0.06
7.076 ± 0.072
9.305 ± 0.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.511 ± 0.025
4.897 ± 0.059
3.641 ± 0.044
3.125 ± 0.038
4.969 ± 0.056
5.828 ± 0.049
4.934 ± 0.046
6.401 ± 0.058
0.992 ± 0.021
3.912 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here