Odrenisrou virus
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I1T355|I1T355_9VIRU Nonstructural protein OS=Odrenisrou virus OX=1048855 PE=4 SV=1
MM1 pKa = 7.19 NAYY4 pKa = 10.24 SYY6 pKa = 10.87 IHH8 pKa = 7.03 DD9 pKa = 4.34 LVVPYY14 pKa = 10.42 YY15 pKa = 10.24 NSPILRR21 pKa = 11.84 DD22 pKa = 3.35 VSVEE26 pKa = 3.63 FRR28 pKa = 11.84 AVNDD32 pKa = 3.71 YY33 pKa = 11.11 LDD35 pKa = 4.6 SPVCVYY41 pKa = 11.15 NSLEE45 pKa = 4.15 FPLHH49 pKa = 5.81 NLSLCTMEE57 pKa = 5.67 SEE59 pKa = 4.71 NLYY62 pKa = 11.03 DD63 pKa = 4.89 CLDD66 pKa = 3.89 SGLMPWRR73 pKa = 11.84 WGPEE77 pKa = 3.77 MFVSRR82 pKa = 11.84 IVTEE86 pKa = 4.13 EE87 pKa = 3.6 TRR89 pKa = 11.84 LFDD92 pKa = 3.9 PFMKK96 pKa = 10.16 QFHH99 pKa = 7.02 LAPLTQILGRR109 pKa = 11.84 GRR111 pKa = 11.84 SGLRR115 pKa = 11.84 SALAWPTGNCSLRR128 pKa = 11.84 FFRR131 pKa = 11.84 QQWGSEE137 pKa = 3.93 SMSFNSSRR145 pKa = 11.84 RR146 pKa = 11.84 LQAQLLFQSTQADD159 pKa = 3.65 NLEE162 pKa = 4.13 IAISALAKK170 pKa = 10.15 DD171 pKa = 4.12 VRR173 pKa = 11.84 GMALSMSFPLNLVPCLDD190 pKa = 3.26 ICKK193 pKa = 10.11 SVSILQFMRR202 pKa = 11.84 VLRR205 pKa = 11.84 SVPEE209 pKa = 3.8 MEE211 pKa = 4.48 MEE213 pKa = 4.14 RR214 pKa = 11.84 EE215 pKa = 3.93 EE216 pKa = 5.22 LGLRR220 pKa = 11.84 DD221 pKa = 4.85 DD222 pKa = 5.3 LDD224 pKa = 3.76 IFLGSVRR231 pKa = 11.84 DD232 pKa = 3.82 TLISDD237 pKa = 4.18 FPGLIEE243 pKa = 4.22 VLTPQDD249 pKa = 4.14 YY250 pKa = 11.16 DD251 pKa = 3.91 SGWDD255 pKa = 3.52 SLSSGEE261 pKa = 5.29 DD262 pKa = 3.42 EE263 pKa = 5.12 VEE265 pKa = 4.06 VV266 pKa = 3.84
Molecular weight: 30.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.457
IPC2_protein 4.469
IPC_protein 4.418
Toseland 4.24
ProMoST 4.533
Dawson 4.38
Bjellqvist 4.52
Wikipedia 4.253
Rodwell 4.253
Grimsley 4.151
Solomon 4.368
Lehninger 4.329
Nozaki 4.482
DTASelect 4.66
Thurlkill 4.253
EMBOSS 4.279
Sillero 4.533
Patrickios 3.63
IPC_peptide 4.38
IPC2_peptide 4.52
IPC2.peptide.svr19 4.472
Protein with the highest isoelectric point:
>tr|I1T355|I1T355_9VIRU Nonstructural protein OS=Odrenisrou virus OX=1048855 PE=4 SV=1
MM1 pKa = 8.0 DD2 pKa = 5.09 NYY4 pKa = 11.43 AKK6 pKa = 10.33 IAIDD10 pKa = 3.68 FAGEE14 pKa = 4.35 PIDD17 pKa = 4.0 PTEE20 pKa = 4.1 LAHH23 pKa = 6.93 LAEE26 pKa = 4.61 EE27 pKa = 4.81 FSFNGFDD34 pKa = 3.93 PNSIMKK40 pKa = 9.96 LIIEE44 pKa = 4.63 RR45 pKa = 11.84 GGATWKK51 pKa = 10.53 EE52 pKa = 4.0 DD53 pKa = 3.49 AKK55 pKa = 11.27 VMIVIGVTRR64 pKa = 11.84 GNKK67 pKa = 7.9 IKK69 pKa = 11.11 KK70 pKa = 9.2 MMDD73 pKa = 2.82 RR74 pKa = 11.84 MSEE77 pKa = 3.8 EE78 pKa = 3.63 GRR80 pKa = 11.84 AKK82 pKa = 10.56 LKK84 pKa = 10.76 QLVDD88 pKa = 3.4 KK89 pKa = 10.97 YY90 pKa = 10.67 KK91 pKa = 10.83 LKK93 pKa = 10.63 EE94 pKa = 4.03 SSPGAKK100 pKa = 10.02 DD101 pKa = 3.33 LTLSRR106 pKa = 11.84 IAAVLAPWTVQALRR120 pKa = 11.84 VLEE123 pKa = 4.25 TKK125 pKa = 10.84 LPVTGATMDD134 pKa = 4.17 SLSQNYY140 pKa = 8.19 PRR142 pKa = 11.84 ALMHH146 pKa = 6.34 SAAGGLLDD154 pKa = 3.68 PTLDD158 pKa = 3.66 EE159 pKa = 4.65 SCFTALLQAHH169 pKa = 6.47 SLYY172 pKa = 10.66 LDD174 pKa = 3.73 CFSRR178 pKa = 11.84 LINPPLRR185 pKa = 11.84 SKK187 pKa = 9.51 TKK189 pKa = 10.44 QEE191 pKa = 3.96 VAQSFSSALQAAYY204 pKa = 10.43 SSNFFSSGQKK214 pKa = 9.85 RR215 pKa = 11.84 QILQHH220 pKa = 6.1 MGLIDD225 pKa = 4.59 GNMKK229 pKa = 9.97 PMKK232 pKa = 10.27 AVIDD236 pKa = 3.72 AAGIYY241 pKa = 10.57 AAMM244 pKa = 5.01
Molecular weight: 26.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.86
IPC2_protein 7.951
IPC_protein 7.98
Toseland 8.521
ProMoST 8.39
Dawson 8.814
Bjellqvist 8.741
Wikipedia 8.858
Rodwell 8.946
Grimsley 8.448
Solomon 9.033
Lehninger 9.019
Nozaki 8.916
DTASelect 8.639
Thurlkill 8.77
EMBOSS 9.019
Sillero 8.96
Patrickios 4.838
IPC_peptide 9.019
IPC2_peptide 7.439
IPC2.peptide.svr19 7.519
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3815
244
2094
953.8
107.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.613 ± 1.123
2.831 ± 0.835
5.374 ± 0.543
6.396 ± 0.488
4.377 ± 0.389
6.186 ± 0.332
2.569 ± 0.266
6.553 ± 0.329
6.265 ± 0.624
9.803 ± 0.598
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.831 ± 0.51
3.801 ± 0.159
4.115 ± 0.171
3.224 ± 0.18
5.19 ± 0.793
9.961 ± 0.489
5.19 ± 0.446
6.396 ± 0.56
1.284 ± 0.153
3.041 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here