Amino acid dipepetide frequency for Streptomyces scopuliridis RB72

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.612AlaAla: 20.612 ± 0.165	0.949AlaCys: 0.949 ± 0.021	8.088AlaAsp: 8.088 ± 0.074	8.983AlaGlu: 8.983 ± 0.074	3.495AlaPhe: 3.495 ± 0.046	13.312AlaGly: 13.312 ± 0.098	2.694AlaHis: 2.694 ± 0.037	3.665AlaIle: 3.665 ± 0.05	2.917AlaLys: 2.917 ± 0.054	14.597AlaLeu: 14.597 ± 0.117	2.484AlaMet: 2.484 ± 0.03	2.001AlaAsn: 2.001 ± 0.036	7.063AlaPro: 7.063 ± 0.078	3.526AlaGln: 3.526 ± 0.048	10.03AlaArg: 10.03 ± 0.076	6.072AlaSer: 6.072 ± 0.053	7.025AlaThr: 7.025 ± 0.056	12.17AlaVal: 12.17 ± 0.084	1.788AlaTrp: 1.788 ± 0.034	2.819AlaTyr: 2.819 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	1.023CysAla: 1.023 ± 0.024	0.087CysCys: 0.087 ± 0.008	0.454CysAsp: 0.454 ± 0.014	0.355CysGlu: 0.355 ± 0.013	0.214CysPhe: 0.214 ± 0.009	0.911CysGly: 0.911 ± 0.023	0.171CysHis: 0.171 ± 0.009	0.147CysIle: 0.147 ± 0.008	0.107CysLys: 0.107 ± 0.008	0.684CysLeu: 0.684 ± 0.019	0.101CysMet: 0.101 ± 0.006	0.127CysAsn: 0.127 ± 0.007	0.437CysPro: 0.437 ± 0.015	0.152CysGln: 0.152 ± 0.009	0.566CysArg: 0.566 ± 0.016	0.428CysSer: 0.428 ± 0.014	0.454CysThr: 0.454 ± 0.015	0.65CysVal: 0.65 ± 0.018	0.112CysTrp: 0.112 ± 0.008	0.149CysTyr: 0.149 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.732AspAla: 7.732 ± 0.072	0.389AspCys: 0.389 ± 0.013	3.422AspAsp: 3.422 ± 0.044	3.893AspGlu: 3.893 ± 0.044	1.651AspPhe: 1.651 ± 0.027	6.63AspGly: 6.63 ± 0.073	1.353AspHis: 1.353 ± 0.024	2.025AspIle: 2.025 ± 0.029	1.204AspLys: 1.204 ± 0.029	5.852AspLeu: 5.852 ± 0.05	0.79AspMet: 0.79 ± 0.019	1.019AspAsn: 1.019 ± 0.021	4.54AspPro: 4.54 ± 0.051	1.546AspGln: 1.546 ± 0.029	4.92AspArg: 4.92 ± 0.052	2.809AspSer: 2.809 ± 0.038	3.288AspThr: 3.288 ± 0.041	4.369AspVal: 4.369 ± 0.052	1.022AspTrp: 1.022 ± 0.023	1.12AspTyr: 1.12 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.247GluAla: 7.247 ± 0.078	0.364GluCys: 0.364 ± 0.013	2.756GluAsp: 2.756 ± 0.036	3.475GluGlu: 3.475 ± 0.05	1.462GluPhe: 1.462 ± 0.027	4.339GluGly: 4.339 ± 0.045	1.389GluHis: 1.389 ± 0.024	2.392GluIle: 2.392 ± 0.031	1.468GluLys: 1.468 ± 0.037	7.044GluLeu: 7.044 ± 0.066	0.903GluMet: 0.903 ± 0.023	1.073GluAsn: 1.073 ± 0.025	3.508GluPro: 3.508 ± 0.054	2.125GluGln: 2.125 ± 0.032	5.776GluArg: 5.776 ± 0.066	2.921GluSer: 2.921 ± 0.041	3.089GluThr: 3.089 ± 0.038	4.365GluVal: 4.365 ± 0.045	0.802GluTrp: 0.802 ± 0.018	1.143GluTyr: 1.143 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	3.627PheAla: 3.627 ± 0.046	0.247PheCys: 0.247 ± 0.01	2.017PheAsp: 2.017 ± 0.034	1.425PheGlu: 1.425 ± 0.027	0.894PhePhe: 0.894 ± 0.022	3.031PheGly: 3.031 ± 0.036	0.598PheHis: 0.598 ± 0.017	0.805PheIle: 0.805 ± 0.02	0.513PheLys: 0.513 ± 0.016	2.627PheLeu: 2.627 ± 0.036	0.415PheMet: 0.415 ± 0.014	0.584PheAsn: 0.584 ± 0.019	1.428PhePro: 1.428 ± 0.028	0.715PheGln: 0.715 ± 0.017	1.842PheArg: 1.842 ± 0.03	1.553PheSer: 1.553 ± 0.03	2.042PheThr: 2.042 ± 0.036	2.194PheVal: 2.194 ± 0.03	0.432PheTrp: 0.432 ± 0.013	0.548PheTyr: 0.548 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	11.468GlyAla: 11.468 ± 0.099	0.791GlyCys: 0.791 ± 0.02	5.196GlyAsp: 5.196 ± 0.056	5.332GlyGlu: 5.332 ± 0.048	2.94GlyPhe: 2.94 ± 0.044	9.194GlyGly: 9.194 ± 0.101	2.263GlyHis: 2.263 ± 0.034	3.626GlyIle: 3.626 ± 0.043	2.484GlyLys: 2.484 ± 0.04	9.605GlyLeu: 9.605 ± 0.079	1.994GlyMet: 1.994 ± 0.033	1.804GlyAsn: 1.804 ± 0.033	5.282GlyPro: 5.282 ± 0.056	2.739GlyGln: 2.739 ± 0.043	7.782GlyArg: 7.782 ± 0.065	5.637GlySer: 5.637 ± 0.067	6.564GlyThr: 6.564 ± 0.07	7.489GlyVal: 7.489 ± 0.073	1.689GlyTrp: 1.689 ± 0.031	2.311GlyTyr: 2.311 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	2.536HisAla: 2.536 ± 0.035	0.192HisCys: 0.192 ± 0.009	1.321HisAsp: 1.321 ± 0.026	1.145HisGlu: 1.145 ± 0.022	0.647HisPhe: 0.647 ± 0.016	2.286HisGly: 2.286 ± 0.032	0.657HisHis: 0.657 ± 0.019	0.671HisIle: 0.671 ± 0.017	0.312HisLys: 0.312 ± 0.011	2.196HisLeu: 2.196 ± 0.033	0.323HisMet: 0.323 ± 0.012	0.365HisAsn: 0.365 ± 0.014	1.692HisPro: 1.692 ± 0.031	0.64HisGln: 0.64 ± 0.017	1.926HisArg: 1.926 ± 0.027	1.047HisSer: 1.047 ± 0.021	1.347HisThr: 1.347 ± 0.024	1.564HisVal: 1.564 ± 0.029	0.348HisTrp: 0.348 ± 0.013	0.467HisTyr: 0.467 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	5.032IleAla: 5.032 ± 0.053	0.319IleCys: 0.319 ± 0.012	2.362IleAsp: 2.362 ± 0.032	2.202IleGlu: 2.202 ± 0.034	0.758IlePhe: 0.758 ± 0.023	3.774IleGly: 3.774 ± 0.045	0.647IleHis: 0.647 ± 0.016	0.941IleIle: 0.941 ± 0.022	0.785IleLys: 0.785 ± 0.021	2.507IleLeu: 2.507 ± 0.04	0.466IleMet: 0.466 ± 0.016	0.747IleAsn: 0.747 ± 0.02	1.865IlePro: 1.865 ± 0.029	0.788IleGln: 0.788 ± 0.02	2.348IleArg: 2.348 ± 0.032	1.886IleSer: 1.886 ± 0.031	2.315IleThr: 2.315 ± 0.037	2.816IleVal: 2.816 ± 0.04	0.414IleTrp: 0.414 ± 0.015	0.572IleTyr: 0.572 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	2.881LysAla: 2.881 ± 0.043	0.119LysCys: 0.119 ± 0.008	1.361LysAsp: 1.361 ± 0.033	1.242LysGlu: 1.242 ± 0.03	0.457LysPhe: 0.457 ± 0.016	1.885LysGly: 1.885 ± 0.037	0.417LysHis: 0.417 ± 0.014	0.909LysIle: 0.909 ± 0.021	0.898LysLys: 0.898 ± 0.029	2.049LysLeu: 2.049 ± 0.038	0.407LysMet: 0.407 ± 0.015	0.576LysAsn: 0.576 ± 0.019	1.39LysPro: 1.39 ± 0.036	0.671LysGln: 0.671 ± 0.018	1.502LysArg: 1.502 ± 0.029	1.235LysSer: 1.235 ± 0.026	1.373LysThr: 1.373 ± 0.032	1.851LysVal: 1.851 ± 0.035	0.265LysTrp: 0.265 ± 0.011	0.457LysTyr: 0.457 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	14.963LeuAla: 14.963 ± 0.113	0.798LeuCys: 0.798 ± 0.02	6.77LeuAsp: 6.77 ± 0.055	4.598LeuGlu: 4.598 ± 0.054	2.725LeuPhe: 2.725 ± 0.041	9.257LeuGly: 9.257 ± 0.079	2.156LeuHis: 2.156 ± 0.031	3.619LeuIle: 3.619 ± 0.049	2.068LeuLys: 2.068 ± 0.033	11.4LeuLeu: 11.4 ± 0.124	1.691LeuMet: 1.691 ± 0.027	1.78LeuAsn: 1.78 ± 0.033	6.447LeuPro: 6.447 ± 0.069	2.107LeuGln: 2.107 ± 0.031	8.659LeuArg: 8.659 ± 0.084	5.609LeuSer: 5.609 ± 0.05	6.969LeuThr: 6.969 ± 0.069	8.501LeuVal: 8.501 ± 0.079	1.252LeuTrp: 1.252 ± 0.027	1.888LeuTyr: 1.888 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	2.278MetAla: 2.278 ± 0.032	0.125MetCys: 0.125 ± 0.009	0.919MetAsp: 0.919 ± 0.021	0.785MetGlu: 0.785 ± 0.019	0.441MetPhe: 0.441 ± 0.014	1.331MetGly: 1.331 ± 0.025	0.324MetHis: 0.324 ± 0.011	0.675MetIle: 0.675 ± 0.02	0.426MetLys: 0.426 ± 0.016	1.687MetLeu: 1.687 ± 0.025	0.308MetMet: 0.308 ± 0.013	0.439MetAsn: 0.439 ± 0.014	1.101MetPro: 1.101 ± 0.022	0.399MetGln: 0.399 ± 0.014	1.42MetArg: 1.42 ± 0.029	1.32MetSer: 1.32 ± 0.022	1.516MetThr: 1.516 ± 0.026	1.313MetVal: 1.313 ± 0.025	0.198MetTrp: 0.198 ± 0.009	0.346MetTyr: 0.346 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.29AsnAla: 2.29 ± 0.037	0.161AsnCys: 0.161 ± 0.009	0.999AsnAsp: 0.999 ± 0.023	0.872AsnGlu: 0.872 ± 0.02	0.491AsnPhe: 0.491 ± 0.015	2.092AsnGly: 2.092 ± 0.038	0.389AsnHis: 0.389 ± 0.013	0.671AsnIle: 0.671 ± 0.019	0.447AsnLys: 0.447 ± 0.015	1.613AsnLeu: 1.613 ± 0.028	0.291AsnMet: 0.291 ± 0.013	0.464AsnAsn: 0.464 ± 0.019	1.408AsnPro: 1.408 ± 0.028	0.553AsnGln: 0.553 ± 0.019	1.253AsnArg: 1.253 ± 0.023	1.001AsnSer: 1.001 ± 0.028	1.197AsnThr: 1.197 ± 0.028	1.374AsnVal: 1.374 ± 0.031	0.297AsnTrp: 0.297 ± 0.014	0.442AsnTyr: 0.442 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	8.693ProAla: 8.693 ± 0.085	0.299ProCys: 0.299 ± 0.011	4.495ProAsp: 4.495 ± 0.049	4.501ProGlu: 4.501 ± 0.058	1.527ProPhe: 1.527 ± 0.027	6.64ProGly: 6.64 ± 0.063	1.304ProHis: 1.304 ± 0.026	1.285ProIle: 1.285 ± 0.025	1.208ProLys: 1.208 ± 0.029	5.404ProLeu: 5.404 ± 0.067	0.983ProMet: 0.983 ± 0.022	0.923ProAsn: 0.923 ± 0.023	3.396ProPro: 3.396 ± 0.056	1.656ProGln: 1.656 ± 0.035	3.955ProArg: 3.955 ± 0.047	3.264ProSer: 3.264 ± 0.047	3.019ProThr: 3.019 ± 0.038	5.555ProVal: 5.555 ± 0.052	0.897ProTrp: 0.897 ± 0.019	1.458ProTyr: 1.458 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	3.333GlnAla: 3.333 ± 0.042	0.156GlnCys: 0.156 ± 0.008	1.409GlnAsp: 1.409 ± 0.028	1.386GlnGlu: 1.386 ± 0.025	0.706GlnPhe: 0.706 ± 0.018	2.237GlnGly: 2.237 ± 0.034	0.606GlnHis: 0.606 ± 0.018	1.111GlnIle: 1.111 ± 0.025	0.613GlnLys: 0.613 ± 0.02	3.166GlnLeu: 3.166 ± 0.037	0.466GlnMet: 0.466 ± 0.013	0.528GlnAsn: 0.528 ± 0.015	1.642GlnPro: 1.642 ± 0.039	1.222GlnGln: 1.222 ± 0.039	2.423GlnArg: 2.423 ± 0.037	1.323GlnSer: 1.323 ± 0.024	1.333GlnThr: 1.333 ± 0.024	2.222GlnVal: 2.222 ± 0.032	0.425GlnTrp: 0.425 ± 0.016	0.626GlnTyr: 0.626 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	9.808ArgAla: 9.808 ± 0.081	0.56ArgCys: 0.56 ± 0.017	4.287ArgAsp: 4.287 ± 0.051	4.842ArgGlu: 4.842 ± 0.046	2.33ArgPhe: 2.33 ± 0.033	5.977ArgGly: 5.977 ± 0.052	1.966ArgHis: 1.966 ± 0.03	3.438ArgIle: 3.438 ± 0.04	1.702ArgLys: 1.702 ± 0.026	8.747ArgLeu: 8.747 ± 0.078	1.674ArgMet: 1.674 ± 0.03	1.357ArgAsn: 1.357 ± 0.026	4.807ArgPro: 4.807 ± 0.052	2.236ArgGln: 2.236 ± 0.034	7.337ArgArg: 7.337 ± 0.078	3.978ArgSer: 3.978 ± 0.045	5.639ArgThr: 5.639 ± 0.058	5.715ArgVal: 5.715 ± 0.053	1.362ArgTrp: 1.362 ± 0.027	1.822ArgTyr: 1.822 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	7.034SerAla: 7.034 ± 0.065	0.416SerCys: 0.416 ± 0.015	2.841SerAsp: 2.841 ± 0.04	2.539SerGlu: 2.539 ± 0.04	1.575SerPhe: 1.575 ± 0.027	6.509SerGly: 6.509 ± 0.061	1.073SerHis: 1.073 ± 0.026	1.525SerIle: 1.525 ± 0.029	1.063SerLys: 1.063 ± 0.022	5.047SerLeu: 5.047 ± 0.052	1.102SerMet: 1.102 ± 0.023	0.914SerAsn: 0.914 ± 0.021	3.236SerPro: 3.236 ± 0.04	1.253SerGln: 1.253 ± 0.025	3.759SerArg: 3.759 ± 0.041	2.992SerSer: 2.992 ± 0.052	3.164SerThr: 3.164 ± 0.041	4.572SerVal: 4.572 ± 0.049	0.933SerTrp: 0.933 ± 0.022	1.29SerTyr: 1.29 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	9.011ThrAla: 9.011 ± 0.075	0.369ThrCys: 0.369 ± 0.014	3.806ThrAsp: 3.806 ± 0.05	3.432ThrGlu: 3.432 ± 0.044	1.629ThrPhe: 1.629 ± 0.028	7.001ThrGly: 7.001 ± 0.061	1.165ThrHis: 1.165 ± 0.022	1.818ThrIle: 1.818 ± 0.035	1.227ThrLys: 1.227 ± 0.029	5.742ThrLeu: 5.742 ± 0.054	0.961ThrMet: 0.961 ± 0.02	1.06ThrAsn: 1.06 ± 0.025	4.078ThrPro: 4.078 ± 0.047	1.346ThrGln: 1.346 ± 0.023	3.801ThrArg: 3.801 ± 0.043	3.305ThrSer: 3.305 ± 0.04	4.006ThrThr: 4.006 ± 0.068	6.152ThrVal: 6.152 ± 0.062	0.844ThrTrp: 0.844 ± 0.023	1.475ThrTyr: 1.475 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	10.34ValAla: 10.34 ± 0.076	0.69ValCys: 0.69 ± 0.017	4.695ValAsp: 4.695 ± 0.051	4.611ValGlu: 4.611 ± 0.048	2.429ValPhe: 2.429 ± 0.036	6.316ValGly: 6.316 ± 0.065	1.786ValHis: 1.786 ± 0.029	3.119ValIle: 3.119 ± 0.046	1.744ValLys: 1.744 ± 0.036	9.414ValLeu: 9.414 ± 0.075	1.459ValMet: 1.459 ± 0.028	1.657ValAsn: 1.657 ± 0.03	5.246ValPro: 5.246 ± 0.056	2.038ValGln: 2.038 ± 0.029	7.078ValArg: 7.078 ± 0.053	4.542ValSer: 4.542 ± 0.05	5.578ValThr: 5.578 ± 0.06	7.652ValVal: 7.652 ± 0.07	1.15ValTrp: 1.15 ± 0.025	1.549ValTyr: 1.549 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.589TrpAla: 1.589 ± 0.026	0.127TrpCys: 0.127 ± 0.007	0.804TrpAsp: 0.804 ± 0.022	0.732TrpGlu: 0.732 ± 0.021	0.522TrpPhe: 0.522 ± 0.017	1.059TrpGly: 1.059 ± 0.024	0.347TrpHis: 0.347 ± 0.012	0.566TrpIle: 0.566 ± 0.018	0.373TrpLys: 0.373 ± 0.013	1.741TrpLeu: 1.741 ± 0.033	0.278TrpMet: 0.278 ± 0.01	0.433TrpAsn: 0.433 ± 0.014	0.793TrpPro: 0.793 ± 0.018	0.6TrpGln: 0.6 ± 0.017	1.343TrpArg: 1.343 ± 0.026	0.945TrpSer: 0.945 ± 0.02	1.051TrpThr: 1.051 ± 0.021	0.925TrpVal: 0.925 ± 0.021	0.327TrpTrp: 0.327 ± 0.012	0.364TrpTyr: 0.364 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.794TyrAla: 2.794 ± 0.038	0.17TyrCys: 0.17 ± 0.009	1.57TyrAsp: 1.57 ± 0.035	1.289TyrGlu: 1.289 ± 0.028	0.644TyrPhe: 0.644 ± 0.018	2.408TyrGly: 2.408 ± 0.032	0.378TyrHis: 0.378 ± 0.014	0.534TyrIle: 0.534 ± 0.018	0.382TyrLys: 0.382 ± 0.016	2.137TyrLeu: 2.137 ± 0.029	0.257TyrMet: 0.257 ± 0.012	0.453TyrAsn: 0.453 ± 0.016	1.106TyrPro: 1.106 ± 0.02	0.621TyrGln: 0.621 ± 0.02	1.867TyrArg: 1.867 ± 0.034	0.968TyrSer: 0.968 ± 0.022	1.259TyrThr: 1.259 ± 0.028	1.642TyrVal: 1.642 ± 0.029	0.364TyrTrp: 0.364 ± 0.013	0.451TyrTyr: 0.451 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6830 proteins (2275614 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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