Garba virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sunrhavirus; Garba sunrhavirus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3R119|A0A0D3R119_9RHAB Glycoprotein OS=Garba virus OX=864696 PE=4 SV=1
MM1 pKa = 7.29NNHH4 pKa = 6.29RR5 pKa = 11.84AKK7 pKa = 10.25EE8 pKa = 3.8IEE10 pKa = 4.4KK11 pKa = 10.17IYY13 pKa = 10.94EE14 pKa = 3.93NLKK17 pKa = 10.53NEE19 pKa = 4.14PSLCGDD25 pKa = 3.82DD26 pKa = 5.38SEE28 pKa = 5.31DD29 pKa = 3.39TGGMYY34 pKa = 10.92GLGMPGTSTGSPLLQSIQMGEE55 pKa = 4.06VHH57 pKa = 7.64DD58 pKa = 4.49SRR60 pKa = 11.84TTSEE64 pKa = 4.14SQKK67 pKa = 10.01PVPWDD72 pKa = 3.28SDD74 pKa = 3.48EE75 pKa = 6.37DD76 pKa = 4.27SDD78 pKa = 4.64TDD80 pKa = 3.56FVYY83 pKa = 10.31PSRR86 pKa = 11.84DD87 pKa = 3.42KK88 pKa = 11.49GCSSDD93 pKa = 5.2DD94 pKa = 3.7EE95 pKa = 4.69NSDD98 pKa = 3.99GEE100 pKa = 4.63TTKK103 pKa = 10.71PSADD107 pKa = 3.67FARR110 pKa = 11.84GGYY113 pKa = 10.51GDD115 pKa = 4.14GFRR118 pKa = 11.84DD119 pKa = 3.71GMRR122 pKa = 11.84EE123 pKa = 3.86AVSKK127 pKa = 10.74INYY130 pKa = 9.43LLTTQGVGLFHH141 pKa = 7.58FDD143 pKa = 3.47GADD146 pKa = 2.93IFIKK150 pKa = 10.69KK151 pKa = 7.37EE152 pKa = 3.73TSLNIEE158 pKa = 3.95NQVDD162 pKa = 4.72GEE164 pKa = 4.5CSLTTCQTDD173 pKa = 3.13QRR175 pKa = 11.84KK176 pKa = 10.1DD177 pKa = 3.17IEE179 pKa = 4.22PRR181 pKa = 11.84EE182 pKa = 4.15MKK184 pKa = 10.0QDD186 pKa = 3.61SASTEE191 pKa = 4.04MDD193 pKa = 3.4SDD195 pKa = 3.69TDD197 pKa = 3.78DD198 pKa = 4.82PEE200 pKa = 5.05LVSSDD205 pKa = 3.67EE206 pKa = 4.0FLEE209 pKa = 4.12ILDD212 pKa = 4.53GGLCVEE218 pKa = 5.36LGGDD222 pKa = 4.11SKK224 pKa = 11.06FLSLDD229 pKa = 3.24SVNGVKK235 pKa = 10.4FEE237 pKa = 4.4EE238 pKa = 4.35NKK240 pKa = 9.53NSKK243 pKa = 7.87MTVVDD248 pKa = 4.32WVLKK252 pKa = 10.63LCTT255 pKa = 4.01

Molecular weight: 28.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3R0Y6|A0A0D3R0Y6_9RHAB Phosphoprotein OS=Garba virus OX=864696 PE=4 SV=1
MM1 pKa = 7.2NRR3 pKa = 11.84LKK5 pKa = 11.01RR6 pKa = 11.84MFGYY10 pKa = 10.71GEE12 pKa = 4.11EE13 pKa = 4.27DD14 pKa = 3.15LADD17 pKa = 3.84FVGIEE22 pKa = 4.21SKK24 pKa = 10.09MVTVVEE30 pKa = 4.27VKK32 pKa = 10.19IQFSLNVTISRR43 pKa = 11.84VGKK46 pKa = 9.45SALSRR51 pKa = 11.84EE52 pKa = 4.4IILKK56 pKa = 10.01EE57 pKa = 3.63LLSNYY62 pKa = 9.58RR63 pKa = 11.84GPSEE67 pKa = 4.35KK68 pKa = 10.19EE69 pKa = 3.49ALFVIGSLLSVPSWIRR85 pKa = 11.84KK86 pKa = 8.99KK87 pKa = 10.76DD88 pKa = 3.3GSGYY92 pKa = 9.75GFRR95 pKa = 11.84GEE97 pKa = 4.11FGIKK101 pKa = 10.21FEE103 pKa = 4.48TDD105 pKa = 3.02NLSLSNNFEE114 pKa = 4.23HH115 pKa = 7.17EE116 pKa = 4.55SISTYY121 pKa = 11.05VKK123 pKa = 10.68DD124 pKa = 3.27NWIVIINSSVKK135 pKa = 8.93TKK137 pKa = 10.33RR138 pKa = 11.84GKK140 pKa = 10.84GGFNPATKK148 pKa = 10.17LQEE151 pKa = 3.96ILSKK155 pKa = 10.98FEE157 pKa = 3.52IRR159 pKa = 11.84TNLFDD164 pKa = 4.37FSPIFIVHH172 pKa = 6.68

Molecular weight: 19.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3663

71

2072

523.3

59.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.331 ± 0.781

2.075 ± 0.297

6.115 ± 0.82

6.416 ± 0.62

5.051 ± 0.366

5.842 ± 0.611

2.239 ± 0.372

7.289 ± 0.776

7.18 ± 0.276

9.801 ± 0.659

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.457 ± 0.247

6.115 ± 0.623

3.74 ± 0.225

3.221 ± 0.374

4.614 ± 0.441

7.835 ± 0.508

5.87 ± 0.389

5.597 ± 0.742

1.611 ± 0.138

3.604 ± 0.409
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski