Amino acid dipepetide frequency for Alphaproteobacteria phage PhiJL001

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.701AlaAla: 9.701 ± 1.057
0.742AlaCys: 0.742 ± 0.235
6.038AlaAsp: 6.038 ± 0.543
5.197AlaGlu: 5.197 ± 0.502
3.514AlaPhe: 3.514 ± 0.443
7.325AlaGly: 7.325 ± 0.703
1.287AlaHis: 1.287 ± 0.269
5.296AlaIle: 5.296 ± 0.598
3.91AlaLys: 3.91 ± 0.557
6.137AlaLeu: 6.137 ± 0.49
2.227AlaMet: 2.227 ± 0.321
4.455AlaAsn: 4.455 ± 0.462
3.118AlaPro: 3.118 ± 0.275
4.108AlaGln: 4.108 ± 0.561
5.593AlaArg: 5.593 ± 0.629
5.049AlaSer: 5.049 ± 0.632
6.88AlaThr: 6.88 ± 0.589
6.731AlaVal: 6.731 ± 0.561
1.237AlaTrp: 1.237 ± 0.2
2.574AlaTyr: 2.574 ± 0.408
0.0AlaXaa: 0.0 ± 0.0
Cys
0.792CysAla: 0.792 ± 0.272
0.049CysCys: 0.049 ± 0.044
0.594CysAsp: 0.594 ± 0.159
0.742CysGlu: 0.742 ± 0.228
0.247CysPhe: 0.247 ± 0.127
0.495CysGly: 0.495 ± 0.158
0.544CysHis: 0.544 ± 0.148
0.346CysIle: 0.346 ± 0.125
0.247CysLys: 0.247 ± 0.098
0.594CysLeu: 0.594 ± 0.17
0.198CysMet: 0.198 ± 0.106
0.643CysAsn: 0.643 ± 0.2
0.396CysPro: 0.396 ± 0.155
0.544CysGln: 0.544 ± 0.19
0.792CysArg: 0.792 ± 0.192
0.495CysSer: 0.495 ± 0.169
0.495CysThr: 0.495 ± 0.165
0.891CysVal: 0.891 ± 0.222
0.148CysTrp: 0.148 ± 0.083
0.445CysTyr: 0.445 ± 0.174
0.0CysXaa: 0.0 ± 0.0
Asp
6.187AspAla: 6.187 ± 0.617
0.495AspCys: 0.495 ± 0.166
5.197AspAsp: 5.197 ± 0.574
4.752AspGlu: 4.752 ± 0.53
2.871AspPhe: 2.871 ± 0.323
6.484AspGly: 6.484 ± 0.55
1.089AspHis: 1.089 ± 0.247
3.366AspIle: 3.366 ± 0.435
2.376AspLys: 2.376 ± 0.414
5.741AspLeu: 5.741 ± 0.526
2.029AspMet: 2.029 ± 0.335
2.475AspAsn: 2.475 ± 0.349
2.673AspPro: 2.673 ± 0.379
2.673AspGln: 2.673 ± 0.375
3.514AspArg: 3.514 ± 0.423
3.712AspSer: 3.712 ± 0.332
3.91AspThr: 3.91 ± 0.419
4.207AspVal: 4.207 ± 0.452
1.633AspTrp: 1.633 ± 0.253
1.485AspTyr: 1.485 ± 0.258
0.0AspXaa: 0.0 ± 0.0
Glu
6.038GluAla: 6.038 ± 0.592
1.237GluCys: 1.237 ± 0.272
4.158GluAsp: 4.158 ± 0.419
3.861GluGlu: 3.861 ± 0.698
2.623GluPhe: 2.623 ± 0.417
4.405GluGly: 4.405 ± 0.476
1.188GluHis: 1.188 ± 0.341
3.663GluIle: 3.663 ± 0.488
3.069GluLys: 3.069 ± 0.462
5.444GluLeu: 5.444 ± 0.613
1.732GluMet: 1.732 ± 0.325
2.128GluAsn: 2.128 ± 0.351
2.376GluPro: 2.376 ± 0.326
2.277GluGln: 2.277 ± 0.34
4.554GluArg: 4.554 ± 0.533
3.118GluSer: 3.118 ± 0.39
4.158GluThr: 4.158 ± 0.403
4.999GluVal: 4.999 ± 0.442
1.336GluTrp: 1.336 ± 0.249
1.237GluTyr: 1.237 ± 0.243
0.0GluXaa: 0.0 ± 0.0
Phe
3.613PheAla: 3.613 ± 0.367
0.396PheCys: 0.396 ± 0.138
3.069PheAsp: 3.069 ± 0.409
2.722PheGlu: 2.722 ± 0.349
1.881PhePhe: 1.881 ± 0.363
3.168PheGly: 3.168 ± 0.485
0.643PheHis: 0.643 ± 0.184
1.683PheIle: 1.683 ± 0.276
1.782PheLys: 1.782 ± 0.373
2.821PheLeu: 2.821 ± 0.443
1.188PheMet: 1.188 ± 0.262
2.475PheAsn: 2.475 ± 0.352
1.534PhePro: 1.534 ± 0.301
1.831PheGln: 1.831 ± 0.416
2.128PheArg: 2.128 ± 0.286
2.326PheSer: 2.326 ± 0.365
2.871PheThr: 2.871 ± 0.447
3.019PheVal: 3.019 ± 0.387
0.99PheTrp: 0.99 ± 0.275
0.643PheTyr: 0.643 ± 0.155
0.0PheXaa: 0.0 ± 0.0
Gly
7.523GlyAla: 7.523 ± 0.792
0.594GlyCys: 0.594 ± 0.157
4.702GlyAsp: 4.702 ± 0.49
5.296GlyGlu: 5.296 ± 0.51
4.158GlyPhe: 4.158 ± 0.54
9.454GlyGly: 9.454 ± 1.384
1.584GlyHis: 1.584 ± 0.269
4.009GlyIle: 4.009 ± 0.439
2.97GlyLys: 2.97 ± 0.453
6.533GlyLeu: 6.533 ± 0.687
1.782GlyMet: 1.782 ± 0.321
3.217GlyAsn: 3.217 ± 0.667
2.574GlyPro: 2.574 ± 0.34
3.118GlyGln: 3.118 ± 0.366
4.95GlyArg: 4.95 ± 0.461
6.533GlySer: 6.533 ± 0.655
6.632GlyThr: 6.632 ± 0.839
5.692GlyVal: 5.692 ± 0.564
1.435GlyTrp: 1.435 ± 0.377
2.475GlyTyr: 2.475 ± 0.331
0.0GlyXaa: 0.0 ± 0.0
His
1.336HisAla: 1.336 ± 0.273
0.346HisCys: 0.346 ± 0.152
1.138HisAsp: 1.138 ± 0.323
1.534HisGlu: 1.534 ± 0.367
0.643HisPhe: 0.643 ± 0.154
1.633HisGly: 1.633 ± 0.34
0.495HisHis: 0.495 ± 0.178
0.792HisIle: 0.792 ± 0.206
0.99HisLys: 0.99 ± 0.223
1.485HisLeu: 1.485 ± 0.274
0.643HisMet: 0.643 ± 0.187
0.445HisAsn: 0.445 ± 0.163
0.94HisPro: 0.94 ± 0.229
0.792HisGln: 0.792 ± 0.198
1.138HisArg: 1.138 ± 0.259
0.891HisSer: 0.891 ± 0.223
0.99HisThr: 0.99 ± 0.254
1.485HisVal: 1.485 ± 0.289
0.396HisTrp: 0.396 ± 0.143
0.693HisTyr: 0.693 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
4.752IleAla: 4.752 ± 0.504
0.643IleCys: 0.643 ± 0.15
3.96IleAsp: 3.96 ± 0.466
2.871IleGlu: 2.871 ± 0.41
1.831IlePhe: 1.831 ± 0.308
3.91IleGly: 3.91 ± 0.384
1.138IleHis: 1.138 ± 0.221
1.584IleIle: 1.584 ± 0.263
2.029IleLys: 2.029 ± 0.344
3.762IleLeu: 3.762 ± 0.505
0.94IleMet: 0.94 ± 0.224
1.98IleAsn: 1.98 ± 0.302
2.376IlePro: 2.376 ± 0.423
1.93IleGln: 1.93 ± 0.273
2.673IleArg: 2.673 ± 0.416
2.871IleSer: 2.871 ± 0.498
3.811IleThr: 3.811 ± 0.571
3.564IleVal: 3.564 ± 0.349
0.742IleTrp: 0.742 ± 0.199
1.138IleTyr: 1.138 ± 0.293
0.0IleXaa: 0.0 ± 0.0
Lys
3.96LysAla: 3.96 ± 0.567
0.445LysCys: 0.445 ± 0.137
2.425LysAsp: 2.425 ± 0.388
3.118LysGlu: 3.118 ± 0.543
2.079LysPhe: 2.079 ± 0.282
3.267LysGly: 3.267 ± 0.502
1.039LysHis: 1.039 ± 0.236
1.782LysIle: 1.782 ± 0.268
2.673LysLys: 2.673 ± 0.493
3.217LysLeu: 3.217 ± 0.411
1.336LysMet: 1.336 ± 0.343
1.534LysAsn: 1.534 ± 0.299
1.683LysPro: 1.683 ± 0.267
1.534LysGln: 1.534 ± 0.242
2.574LysArg: 2.574 ± 0.324
1.881LysSer: 1.881 ± 0.37
2.623LysThr: 2.623 ± 0.373
2.326LysVal: 2.326 ± 0.391
0.742LysTrp: 0.742 ± 0.184
1.485LysTyr: 1.485 ± 0.269
0.0LysXaa: 0.0 ± 0.0
Leu
7.177LeuAla: 7.177 ± 0.527
0.693LeuCys: 0.693 ± 0.227
5.791LeuAsp: 5.791 ± 0.651
5.345LeuGlu: 5.345 ± 0.673
3.415LeuPhe: 3.415 ± 0.32
6.583LeuGly: 6.583 ± 0.622
0.94LeuHis: 0.94 ± 0.259
3.415LeuIle: 3.415 ± 0.489
2.772LeuLys: 2.772 ± 0.443
5.494LeuLeu: 5.494 ± 0.529
1.534LeuMet: 1.534 ± 0.277
2.92LeuAsn: 2.92 ± 0.376
4.603LeuPro: 4.603 ± 0.528
3.168LeuGln: 3.168 ± 0.494
5.543LeuArg: 5.543 ± 0.544
5.543LeuSer: 5.543 ± 0.482
5.543LeuThr: 5.543 ± 0.656
4.801LeuVal: 4.801 ± 0.468
1.386LeuTrp: 1.386 ± 0.234
2.029LeuTyr: 2.029 ± 0.36
0.0LeuXaa: 0.0 ± 0.0
Met
2.376MetAla: 2.376 ± 0.368
0.148MetCys: 0.148 ± 0.088
1.287MetAsp: 1.287 ± 0.236
1.881MetGlu: 1.881 ± 0.275
0.841MetPhe: 0.841 ± 0.189
1.732MetGly: 1.732 ± 0.369
0.247MetHis: 0.247 ± 0.098
0.841MetIle: 0.841 ± 0.18
1.039MetLys: 1.039 ± 0.218
1.782MetLeu: 1.782 ± 0.32
0.99MetMet: 0.99 ± 0.246
0.99MetAsn: 0.99 ± 0.24
1.188MetPro: 1.188 ± 0.303
0.841MetGln: 0.841 ± 0.189
1.534MetArg: 1.534 ± 0.275
1.881MetSer: 1.881 ± 0.272
1.485MetThr: 1.485 ± 0.28
1.782MetVal: 1.782 ± 0.333
0.445MetTrp: 0.445 ± 0.169
0.742MetTyr: 0.742 ± 0.18
0.0MetXaa: 0.0 ± 0.0
Asn
3.811AsnAla: 3.811 ± 0.475
0.297AsnCys: 0.297 ± 0.143
2.277AsnAsp: 2.277 ± 0.455
1.831AsnGlu: 1.831 ± 0.232
1.386AsnPhe: 1.386 ± 0.264
4.405AsnGly: 4.405 ± 0.457
0.643AsnHis: 0.643 ± 0.217
1.633AsnIle: 1.633 ± 0.229
1.881AsnLys: 1.881 ± 0.29
3.712AsnLeu: 3.712 ± 0.586
0.891AsnMet: 0.891 ± 0.184
2.475AsnAsn: 2.475 ± 0.539
2.079AsnPro: 2.079 ± 0.339
1.435AsnGln: 1.435 ± 0.296
2.524AsnArg: 2.524 ± 0.374
2.475AsnSer: 2.475 ± 0.332
2.277AsnThr: 2.277 ± 0.382
3.366AsnVal: 3.366 ± 0.377
0.643AsnTrp: 0.643 ± 0.208
0.643AsnTyr: 0.643 ± 0.201
0.0AsnXaa: 0.0 ± 0.0
Pro
3.415ProAla: 3.415 ± 0.424
0.297ProCys: 0.297 ± 0.121
3.118ProAsp: 3.118 ± 0.359
2.821ProGlu: 2.821 ± 0.381
1.881ProPhe: 1.881 ± 0.303
3.069ProGly: 3.069 ± 0.444
0.643ProHis: 0.643 ± 0.19
1.683ProIle: 1.683 ± 0.26
1.534ProLys: 1.534 ± 0.304
3.564ProLeu: 3.564 ± 0.448
0.495ProMet: 0.495 ± 0.139
1.93ProAsn: 1.93 ± 0.266
2.376ProPro: 2.376 ± 0.401
1.336ProGln: 1.336 ± 0.336
1.93ProArg: 1.93 ± 0.314
2.524ProSer: 2.524 ± 0.427
3.712ProThr: 3.712 ± 0.348
2.772ProVal: 2.772 ± 0.276
0.742ProTrp: 0.742 ± 0.19
1.633ProTyr: 1.633 ± 0.266
0.0ProXaa: 0.0 ± 0.0
Gln
4.306GlnAla: 4.306 ± 0.546
0.247GlnCys: 0.247 ± 0.108
1.98GlnAsp: 1.98 ± 0.339
1.98GlnGlu: 1.98 ± 0.381
1.237GlnPhe: 1.237 ± 0.279
2.92GlnGly: 2.92 ± 0.342
1.138GlnHis: 1.138 ± 0.308
2.524GlnIle: 2.524 ± 0.452
1.584GlnLys: 1.584 ± 0.254
3.663GlnLeu: 3.663 ± 0.56
1.089GlnMet: 1.089 ± 0.232
1.039GlnAsn: 1.039 ± 0.203
1.584GlnPro: 1.584 ± 0.265
1.435GlnGln: 1.435 ± 0.297
2.425GlnArg: 2.425 ± 0.426
2.623GlnSer: 2.623 ± 0.285
2.574GlnThr: 2.574 ± 0.332
3.118GlnVal: 3.118 ± 0.349
0.792GlnTrp: 0.792 ± 0.196
0.643GlnTyr: 0.643 ± 0.171
0.0GlnXaa: 0.0 ± 0.0
Arg
4.851ArgAla: 4.851 ± 0.569
0.643ArgCys: 0.643 ± 0.194
4.108ArgAsp: 4.108 ± 0.441
5.098ArgGlu: 5.098 ± 0.666
2.376ArgPhe: 2.376 ± 0.346
4.405ArgGly: 4.405 ± 0.507
1.336ArgHis: 1.336 ± 0.282
3.613ArgIle: 3.613 ± 0.4
2.673ArgLys: 2.673 ± 0.454
5.197ArgLeu: 5.197 ± 0.636
1.237ArgMet: 1.237 ± 0.266
1.98ArgAsn: 1.98 ± 0.29
2.079ArgPro: 2.079 ± 0.321
3.069ArgGln: 3.069 ± 0.462
4.851ArgArg: 4.851 ± 0.672
3.316ArgSer: 3.316 ± 0.456
3.564ArgThr: 3.564 ± 0.353
5.642ArgVal: 5.642 ± 0.618
1.534ArgTrp: 1.534 ± 0.293
1.435ArgTyr: 1.435 ± 0.304
0.0ArgXaa: 0.0 ± 0.0
Ser
4.9SerAla: 4.9 ± 0.59
0.742SerCys: 0.742 ± 0.181
3.762SerAsp: 3.762 ± 0.423
3.564SerGlu: 3.564 ± 0.514
2.97SerPhe: 2.97 ± 0.379
6.583SerGly: 6.583 ± 0.695
0.693SerHis: 0.693 ± 0.165
2.821SerIle: 2.821 ± 0.393
2.326SerLys: 2.326 ± 0.286
4.554SerLeu: 4.554 ± 0.436
1.039SerMet: 1.039 ± 0.241
2.326SerAsn: 2.326 ± 0.341
2.376SerPro: 2.376 ± 0.355
2.128SerGln: 2.128 ± 0.31
3.316SerArg: 3.316 ± 0.376
3.564SerSer: 3.564 ± 0.505
4.158SerThr: 4.158 ± 0.636
4.059SerVal: 4.059 ± 0.446
1.534SerTrp: 1.534 ± 0.35
1.683SerTyr: 1.683 ± 0.259
0.0SerXaa: 0.0 ± 0.0
Thr
6.335ThrAla: 6.335 ± 0.681
0.297ThrCys: 0.297 ± 0.109
4.603ThrAsp: 4.603 ± 0.499
3.465ThrGlu: 3.465 ± 0.361
3.069ThrPhe: 3.069 ± 0.43
6.583ThrGly: 6.583 ± 0.647
1.089ThrHis: 1.089 ± 0.237
4.108ThrIle: 4.108 ± 0.642
2.722ThrLys: 2.722 ± 0.412
6.335ThrLeu: 6.335 ± 0.713
1.633ThrMet: 1.633 ± 0.222
2.524ThrAsn: 2.524 ± 0.403
2.673ThrPro: 2.673 ± 0.476
2.524ThrGln: 2.524 ± 0.443
4.158ThrArg: 4.158 ± 0.583
4.207ThrSer: 4.207 ± 0.558
6.434ThrThr: 6.434 ± 0.786
5.939ThrVal: 5.939 ± 0.695
0.99ThrTrp: 0.99 ± 0.291
1.881ThrTyr: 1.881 ± 0.402
0.0ThrXaa: 0.0 ± 0.0
Val
6.731ValAla: 6.731 ± 0.545
0.792ValCys: 0.792 ± 0.221
4.95ValAsp: 4.95 ± 0.523
4.455ValGlu: 4.455 ± 0.467
2.178ValPhe: 2.178 ± 0.349
5.791ValGly: 5.791 ± 0.612
1.782ValHis: 1.782 ± 0.289
2.722ValIle: 2.722 ± 0.375
3.069ValLys: 3.069 ± 0.365
5.296ValLeu: 5.296 ± 0.478
1.93ValMet: 1.93 ± 0.317
2.871ValAsn: 2.871 ± 0.427
3.415ValPro: 3.415 ± 0.403
2.623ValGln: 2.623 ± 0.372
5.296ValArg: 5.296 ± 0.52
4.306ValSer: 4.306 ± 0.503
6.187ValThr: 6.187 ± 0.646
4.356ValVal: 4.356 ± 0.525
1.386ValTrp: 1.386 ± 0.227
1.633ValTyr: 1.633 ± 0.235
0.0ValXaa: 0.0 ± 0.0
Trp
1.386TrpAla: 1.386 ± 0.261
0.396TrpCys: 0.396 ± 0.137
1.881TrpAsp: 1.881 ± 0.33
1.435TrpGlu: 1.435 ± 0.289
0.643TrpPhe: 0.643 ± 0.183
1.336TrpGly: 1.336 ± 0.225
0.594TrpHis: 0.594 ± 0.159
0.742TrpIle: 0.742 ± 0.171
1.188TrpLys: 1.188 ± 0.248
1.435TrpLeu: 1.435 ± 0.232
0.396TrpMet: 0.396 ± 0.158
0.594TrpAsn: 0.594 ± 0.174
0.594TrpPro: 0.594 ± 0.166
0.544TrpGln: 0.544 ± 0.166
1.782TrpArg: 1.782 ± 0.355
0.594TrpSer: 0.594 ± 0.177
1.039TrpThr: 1.039 ± 0.217
1.336TrpVal: 1.336 ± 0.242
0.445TrpTrp: 0.445 ± 0.165
0.544TrpTyr: 0.544 ± 0.171
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.93TyrAla: 1.93 ± 0.338
0.198TyrCys: 0.198 ± 0.099
1.831TyrAsp: 1.831 ± 0.334
1.633TyrGlu: 1.633 ± 0.291
0.94TyrPhe: 0.94 ± 0.204
1.683TyrGly: 1.683 ± 0.394
0.693TyrHis: 0.693 ± 0.224
1.881TyrIle: 1.881 ± 0.288
0.891TyrLys: 0.891 ± 0.193
1.93TyrLeu: 1.93 ± 0.281
0.693TyrMet: 0.693 ± 0.186
1.683TyrAsn: 1.683 ± 0.268
0.841TyrPro: 0.841 ± 0.184
0.99TyrGln: 0.99 ± 0.258
1.732TyrArg: 1.732 ± 0.349
1.237TyrSer: 1.237 ± 0.299
2.227TyrThr: 2.227 ± 0.315
1.683TyrVal: 1.683 ± 0.299
0.346TyrTrp: 0.346 ± 0.129
0.445TyrTyr: 0.445 ± 0.155
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 90 proteins (20205 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski