Listeria phage LP-101
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A059T5F7|A0A059T5F7_9CAUD Uncharacterized protein OS=Listeria phage LP-101 OX=1458856 GN=LP101_029 PE=4 SV=1
MM1 pKa = 7.8 TITTAQRR8 pKa = 11.84 KK9 pKa = 8.41 YY10 pKa = 10.45 NEE12 pKa = 4.21 AMHH15 pKa = 6.4 EE16 pKa = 4.48 FINMVDD22 pKa = 4.21 DD23 pKa = 4.66 FEE25 pKa = 5.63 EE26 pKa = 4.32 STPDD30 pKa = 3.28 FAKK33 pKa = 10.58 EE34 pKa = 3.83 VLHH37 pKa = 6.86 DD38 pKa = 3.74 CDD40 pKa = 3.79 YY41 pKa = 11.79 VVVTKK46 pKa = 10.72 NEE48 pKa = 3.88 KK49 pKa = 10.12 YY50 pKa = 10.5 AVALCTLSTDD60 pKa = 2.97 EE61 pKa = 5.77 CEE63 pKa = 4.31 YY64 pKa = 10.31 DD65 pKa = 3.25 TNLYY69 pKa = 10.48 LDD71 pKa = 4.53 EE72 pKa = 5.25 KK73 pKa = 10.61 LVDD76 pKa = 3.69 YY77 pKa = 9.7 STVNVNGVTYY87 pKa = 9.56 YY88 pKa = 10.6 INIVEE93 pKa = 4.5 TNDD96 pKa = 3.41 IDD98 pKa = 4.28 DD99 pKa = 5.11 LEE101 pKa = 4.67 IATDD105 pKa = 3.74 EE106 pKa = 5.5 DD107 pKa = 4.21 EE108 pKa = 4.55 MKK110 pKa = 10.7 SDD112 pKa = 3.5 NQEE115 pKa = 4.16 IILKK119 pKa = 10.28 SEE121 pKa = 4.26 LKK123 pKa = 10.66
Molecular weight: 14.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.681
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.77
Rodwell 3.719
Grimsley 3.592
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.164
Thurlkill 3.732
EMBOSS 3.783
Sillero 3.999
Patrickios 1.074
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.885
Protein with the highest isoelectric point:
>tr|A0A059T7Z9|A0A059T7Z9_9CAUD Uncharacterized protein OS=Listeria phage LP-101 OX=1458856 GN=LP101_051 PE=4 SV=1
MM1 pKa = 7.7 RR2 pKa = 11.84 GIEE5 pKa = 3.99 FRR7 pKa = 11.84 GKK9 pKa = 9.6 IVGAEE14 pKa = 3.85 GFVYY18 pKa = 10.12 GKK20 pKa = 10.52 LLAPLASGNAYY31 pKa = 9.31 IAYY34 pKa = 9.53 DD35 pKa = 3.54 VNEE38 pKa = 3.74 VRR40 pKa = 11.84 RR41 pKa = 11.84 FSKK44 pKa = 9.55 GTYY47 pKa = 9.34 AGRR50 pKa = 11.84 NTMSATALLNLKK62 pKa = 9.81 KK63 pKa = 10.6 VSSYY67 pKa = 10.32 MCGKK71 pKa = 8.94 TSQRR75 pKa = 11.84 TCGKK79 pKa = 9.77 LLIVLRR85 pKa = 11.84 FTVIYY90 pKa = 8.63 TKK92 pKa = 10.17 IWICWRR98 pKa = 11.84 RR99 pKa = 11.84 NNGIRR104 pKa = 11.84 KK105 pKa = 9.18 FKK107 pKa = 10.27 ISNRR111 pKa = 11.84 GDD113 pKa = 3.02 FRR115 pKa = 11.84 RR116 pKa = 11.84 YY117 pKa = 9.55 IFNTYY122 pKa = 8.16 FKK124 pKa = 10.08 KK125 pKa = 9.6 WRR127 pKa = 11.84 YY128 pKa = 7.14 EE129 pKa = 3.7
Molecular weight: 15.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 10.028
IPC_protein 10.643
Toseland 10.628
ProMoST 10.321
Dawson 10.774
Bjellqvist 10.467
Wikipedia 10.965
Rodwell 11.125
Grimsley 10.833
Solomon 10.833
Lehninger 10.804
Nozaki 10.613
DTASelect 10.467
Thurlkill 10.643
EMBOSS 11.023
Sillero 10.687
Patrickios 10.818
IPC_peptide 10.833
IPC2_peptide 9.458
IPC2.peptide.svr19 8.263
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
70
0
70
13278
36
1639
189.7
21.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.869 ± 0.628
0.859 ± 0.129
6.356 ± 0.281
7.991 ± 0.455
4.398 ± 0.334
6.063 ± 0.478
1.551 ± 0.172
6.853 ± 0.262
9.173 ± 0.389
8.254 ± 0.3
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.342 ± 0.126
6.334 ± 0.192
2.478 ± 0.17
3.382 ± 0.24
3.532 ± 0.283
6.161 ± 0.373
5.859 ± 0.312
6.146 ± 0.239
1.326 ± 0.124
4.074 ± 0.323
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here