Amino acid dipepetide frequency for Aquabacterium sp. NJ1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.892AlaAla: 14.892 ± 0.237
1.382AlaCys: 1.382 ± 0.036
6.401AlaAsp: 6.401 ± 0.104
6.054AlaGlu: 6.054 ± 0.089
3.908AlaPhe: 3.908 ± 0.062
9.238AlaGly: 9.238 ± 0.114
2.883AlaHis: 2.883 ± 0.06
5.025AlaIle: 5.025 ± 0.098
4.184AlaLys: 4.184 ± 0.081
13.901AlaLeu: 13.901 ± 0.24
3.71AlaMet: 3.71 ± 0.071
3.0AlaAsn: 3.0 ± 0.056
5.643AlaPro: 5.643 ± 0.117
6.348AlaGln: 6.348 ± 0.156
7.919AlaArg: 7.919 ± 0.113
7.251AlaSer: 7.251 ± 0.09
5.9AlaThr: 5.9 ± 0.101
8.348AlaVal: 8.348 ± 0.096
1.945AlaTrp: 1.945 ± 0.052
2.629AlaTyr: 2.629 ± 0.058
0.0AlaXaa: 0.0 ± 0.0
Cys
1.106CysAla: 1.106 ± 0.034
0.127CysCys: 0.127 ± 0.01
0.536CysAsp: 0.536 ± 0.022
0.497CysGlu: 0.497 ± 0.022
0.301CysPhe: 0.301 ± 0.015
0.966CysGly: 0.966 ± 0.035
0.301CysHis: 0.301 ± 0.039
0.414CysIle: 0.414 ± 0.019
0.298CysLys: 0.298 ± 0.016
0.92CysLeu: 0.92 ± 0.031
0.241CysMet: 0.241 ± 0.014
0.243CysAsn: 0.243 ± 0.013
0.529CysPro: 0.529 ± 0.02
0.339CysGln: 0.339 ± 0.016
0.531CysArg: 0.531 ± 0.022
0.494CysSer: 0.494 ± 0.021
0.489CysThr: 0.489 ± 0.024
0.74CysVal: 0.74 ± 0.025
0.121CysTrp: 0.121 ± 0.01
0.215CysTyr: 0.215 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
6.664AspAla: 6.664 ± 0.091
0.464AspCys: 0.464 ± 0.02
2.807AspAsp: 2.807 ± 0.062
3.39AspGlu: 3.39 ± 0.053
1.99AspPhe: 1.99 ± 0.036
4.408AspGly: 4.408 ± 0.121
1.236AspHis: 1.236 ± 0.037
2.644AspIle: 2.644 ± 0.059
2.26AspLys: 2.26 ± 0.051
5.444AspLeu: 5.444 ± 0.072
1.328AspMet: 1.328 ± 0.03
1.388AspAsn: 1.388 ± 0.038
2.807AspPro: 2.807 ± 0.045
2.157AspGln: 2.157 ± 0.068
3.037AspArg: 3.037 ± 0.06
2.292AspSer: 2.292 ± 0.057
2.901AspThr: 2.901 ± 0.075
4.263AspVal: 4.263 ± 0.066
0.948AspTrp: 0.948 ± 0.03
1.337AspTyr: 1.337 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
6.962GluAla: 6.962 ± 0.084
0.372GluCys: 0.372 ± 0.018
2.242GluAsp: 2.242 ± 0.046
2.407GluGlu: 2.407 ± 0.053
1.624GluPhe: 1.624 ± 0.043
3.898GluGly: 3.898 ± 0.071
1.5GluHis: 1.5 ± 0.041
2.423GluIle: 2.423 ± 0.053
1.71GluLys: 1.71 ± 0.047
5.704GluLeu: 5.704 ± 0.085
1.209GluMet: 1.209 ± 0.033
1.102GluAsn: 1.102 ± 0.038
2.5GluPro: 2.5 ± 0.051
2.816GluGln: 2.816 ± 0.05
4.059GluArg: 4.059 ± 0.07
2.331GluSer: 2.331 ± 0.051
2.397GluThr: 2.397 ± 0.058
4.11GluVal: 4.11 ± 0.067
0.684GluTrp: 0.684 ± 0.025
0.994GluTyr: 0.994 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
3.612PheAla: 3.612 ± 0.059
0.354PheCys: 0.354 ± 0.019
2.407PheAsp: 2.407 ± 0.041
2.055PheGlu: 2.055 ± 0.042
1.268PhePhe: 1.268 ± 0.038
3.122PheGly: 3.122 ± 0.059
0.732PheHis: 0.732 ± 0.028
1.504PheIle: 1.504 ± 0.034
1.576PheLys: 1.576 ± 0.041
2.77PheLeu: 2.77 ± 0.048
1.003PheMet: 1.003 ± 0.032
1.305PheAsn: 1.305 ± 0.042
1.335PhePro: 1.335 ± 0.035
1.109PheGln: 1.109 ± 0.03
1.582PheArg: 1.582 ± 0.038
2.081PheSer: 2.081 ± 0.043
1.894PheThr: 1.894 ± 0.042
2.689PheVal: 2.689 ± 0.048
0.507PheTrp: 0.507 ± 0.023
0.889PheTyr: 0.889 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
8.241GlyAla: 8.241 ± 0.102
0.889GlyCys: 0.889 ± 0.028
3.907GlyAsp: 3.907 ± 0.079
4.116GlyGlu: 4.116 ± 0.062
3.018GlyPhe: 3.018 ± 0.048
6.662GlyGly: 6.662 ± 0.132
2.126GlyHis: 2.126 ± 0.055
3.663GlyIle: 3.663 ± 0.064
3.495GlyLys: 3.495 ± 0.07
9.258GlyLeu: 9.258 ± 0.107
2.4GlyMet: 2.4 ± 0.051
2.305GlyAsn: 2.305 ± 0.066
2.972GlyPro: 2.972 ± 0.058
4.076GlyGln: 4.076 ± 0.077
4.8GlyArg: 4.8 ± 0.067
4.658GlySer: 4.658 ± 0.108
4.386GlyThr: 4.386 ± 0.138
6.339GlyVal: 6.339 ± 0.079
1.445GlyTrp: 1.445 ± 0.039
2.113GlyTyr: 2.113 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
2.998HisAla: 2.998 ± 0.061
0.253HisCys: 0.253 ± 0.015
1.282HisAsp: 1.282 ± 0.04
1.336HisGlu: 1.336 ± 0.041
0.982HisPhe: 0.982 ± 0.029
2.166HisGly: 2.166 ± 0.052
0.825HisHis: 0.825 ± 0.036
1.088HisIle: 1.088 ± 0.037
0.728HisLys: 0.728 ± 0.025
2.412HisLeu: 2.412 ± 0.051
0.595HisMet: 0.595 ± 0.026
0.631HisAsn: 0.631 ± 0.025
1.521HisPro: 1.521 ± 0.033
0.919HisGln: 0.919 ± 0.031
1.413HisArg: 1.413 ± 0.037
1.057HisSer: 1.057 ± 0.036
1.27HisThr: 1.27 ± 0.035
1.768HisVal: 1.768 ± 0.038
0.452HisTrp: 0.452 ± 0.034
0.654HisTyr: 0.654 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
5.091IleAla: 5.091 ± 0.079
0.4IleCys: 0.4 ± 0.02
3.01IleAsp: 3.01 ± 0.048
3.21IleGlu: 3.21 ± 0.064
1.214IlePhe: 1.214 ± 0.034
3.955IleGly: 3.955 ± 0.06
0.955IleHis: 0.955 ± 0.032
1.635IleIle: 1.635 ± 0.043
1.935IleLys: 1.935 ± 0.05
3.376IleLeu: 3.376 ± 0.054
0.965IleMet: 0.965 ± 0.033
1.623IleAsn: 1.623 ± 0.043
2.011IlePro: 2.011 ± 0.046
1.685IleGln: 1.685 ± 0.038
2.466IleArg: 2.466 ± 0.042
2.605IleSer: 2.605 ± 0.062
2.847IleThr: 2.847 ± 0.062
3.366IleVal: 3.366 ± 0.063
0.576IleTrp: 0.576 ± 0.022
0.991IleTyr: 0.991 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
4.887LysAla: 4.887 ± 0.092
0.2LysCys: 0.2 ± 0.014
1.972LysAsp: 1.972 ± 0.049
1.666LysGlu: 1.666 ± 0.047
0.968LysPhe: 0.968 ± 0.031
3.03LysGly: 3.03 ± 0.061
0.785LysHis: 0.785 ± 0.027
1.485LysIle: 1.485 ± 0.042
1.443LysLys: 1.443 ± 0.057
4.108LysLeu: 4.108 ± 0.073
0.88LysMet: 0.88 ± 0.028
0.924LysAsn: 0.924 ± 0.032
2.376LysPro: 2.376 ± 0.053
1.579LysGln: 1.579 ± 0.043
2.332LysArg: 2.332 ± 0.053
1.798LysSer: 1.798 ± 0.049
1.983LysThr: 1.983 ± 0.045
3.051LysVal: 3.051 ± 0.066
0.414LysTrp: 0.414 ± 0.022
0.674LysTyr: 0.674 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
13.755LeuAla: 13.755 ± 0.16
0.986LeuCys: 0.986 ± 0.031
6.017LeuAsp: 6.017 ± 0.072
4.683LeuGlu: 4.683 ± 0.079
3.334LeuPhe: 3.334 ± 0.06
8.41LeuGly: 8.41 ± 0.092
2.503LeuHis: 2.503 ± 0.052
4.789LeuIle: 4.789 ± 0.068
4.24LeuLys: 4.24 ± 0.07
10.606LeuLeu: 10.606 ± 0.134
2.796LeuMet: 2.796 ± 0.056
3.308LeuAsn: 3.308 ± 0.061
6.045LeuPro: 6.045 ± 0.114
4.448LeuGln: 4.448 ± 0.077
6.887LeuArg: 6.887 ± 0.106
7.105LeuSer: 7.105 ± 0.101
5.978LeuThr: 5.978 ± 0.088
7.376LeuVal: 7.376 ± 0.093
1.336LeuTrp: 1.336 ± 0.037
2.091LeuTyr: 2.091 ± 0.044
0.0LeuXaa: 0.0 ± 0.0
Met
3.535MetAla: 3.535 ± 0.051
0.215MetCys: 0.215 ± 0.012
1.311MetAsp: 1.311 ± 0.032
1.031MetGlu: 1.031 ± 0.03
0.72MetPhe: 0.72 ± 0.025
2.241MetGly: 2.241 ± 0.05
0.544MetHis: 0.544 ± 0.024
1.045MetIle: 1.045 ± 0.031
1.049MetLys: 1.049 ± 0.037
2.856MetLeu: 2.856 ± 0.053
0.674MetMet: 0.674 ± 0.025
0.833MetAsn: 0.833 ± 0.029
1.594MetPro: 1.594 ± 0.039
1.168MetGln: 1.168 ± 0.031
1.721MetArg: 1.721 ± 0.042
1.947MetSer: 1.947 ± 0.041
1.541MetThr: 1.541 ± 0.039
1.92MetVal: 1.92 ± 0.038
0.298MetTrp: 0.298 ± 0.016
0.413MetTyr: 0.413 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
3.208AsnAla: 3.208 ± 0.063
0.282AsnCys: 0.282 ± 0.018
1.518AsnAsp: 1.518 ± 0.068
1.385AsnGlu: 1.385 ± 0.038
0.941AsnPhe: 0.941 ± 0.029
2.43AsnGly: 2.43 ± 0.057
0.549AsnHis: 0.549 ± 0.022
1.25AsnIle: 1.25 ± 0.048
1.04AsnLys: 1.04 ± 0.033
2.857AsnLeu: 2.857 ± 0.053
0.68AsnMet: 0.68 ± 0.027
0.868AsnAsn: 0.868 ± 0.042
1.851AsnPro: 1.851 ± 0.046
1.153AsnGln: 1.153 ± 0.033
1.534AsnArg: 1.534 ± 0.035
1.322AsnSer: 1.322 ± 0.051
1.745AsnThr: 1.745 ± 0.062
2.077AsnVal: 2.077 ± 0.056
0.534AsnTrp: 0.534 ± 0.023
0.7AsnTyr: 0.7 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
6.389ProAla: 6.389 ± 0.109
0.369ProCys: 0.369 ± 0.02
3.327ProAsp: 3.327 ± 0.052
3.214ProGlu: 3.214 ± 0.063
1.666ProPhe: 1.666 ± 0.038
4.233ProGly: 4.233 ± 0.07
1.2ProHis: 1.2 ± 0.032
2.077ProIle: 2.077 ± 0.038
1.708ProLys: 1.708 ± 0.044
5.097ProLeu: 5.097 ± 0.095
1.373ProMet: 1.373 ± 0.033
1.357ProAsn: 1.357 ± 0.035
2.402ProPro: 2.402 ± 0.062
2.214ProGln: 2.214 ± 0.048
2.682ProArg: 2.682 ± 0.054
2.922ProSer: 2.922 ± 0.053
2.835ProThr: 2.835 ± 0.064
4.247ProVal: 4.247 ± 0.07
0.832ProTrp: 0.832 ± 0.029
1.17ProTyr: 1.17 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
7.22GlnAla: 7.22 ± 0.143
0.345GlnCys: 0.345 ± 0.017
2.031GlnAsp: 2.031 ± 0.045
1.758GlnGlu: 1.758 ± 0.043
1.341GlnPhe: 1.341 ± 0.032
3.78GlnGly: 3.78 ± 0.069
1.185GlnHis: 1.185 ± 0.034
2.034GlnIle: 2.034 ± 0.066
1.203GlnLys: 1.203 ± 0.032
4.61GlnLeu: 4.61 ± 0.068
1.075GlnMet: 1.075 ± 0.032
0.846GlnAsn: 0.846 ± 0.027
2.463GlnPro: 2.463 ± 0.057
2.268GlnGln: 2.268 ± 0.054
3.436GlnArg: 3.436 ± 0.067
2.305GlnSer: 2.305 ± 0.049
2.342GlnThr: 2.342 ± 0.055
3.433GlnVal: 3.433 ± 0.08
0.754GlnTrp: 0.754 ± 0.028
0.891GlnTyr: 0.891 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
6.469ArgAla: 6.469 ± 0.098
0.554ArgCys: 0.554 ± 0.024
3.297ArgAsp: 3.297 ± 0.059
3.701ArgGlu: 3.701 ± 0.064
2.566ArgPhe: 2.566 ± 0.048
3.882ArgGly: 3.882 ± 0.062
1.85ArgHis: 1.85 ± 0.054
3.084ArgIle: 3.084 ± 0.053
2.128ArgLys: 2.128 ± 0.055
7.279ArgLeu: 7.279 ± 0.108
1.805ArgMet: 1.805 ± 0.036
1.717ArgAsn: 1.717 ± 0.041
2.88ArgPro: 2.88 ± 0.05
3.396ArgGln: 3.396 ± 0.059
4.156ArgArg: 4.156 ± 0.07
3.111ArgSer: 3.111 ± 0.06
3.049ArgThr: 3.049 ± 0.057
4.842ArgVal: 4.842 ± 0.076
1.113ArgTrp: 1.113 ± 0.039
1.745ArgTyr: 1.745 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
6.541SerAla: 6.541 ± 0.122
0.531SerCys: 0.531 ± 0.03
2.773SerAsp: 2.773 ± 0.055
2.548SerGlu: 2.548 ± 0.052
2.075SerPhe: 2.075 ± 0.043
5.104SerGly: 5.104 ± 0.074
1.291SerHis: 1.291 ± 0.035
2.418SerIle: 2.418 ± 0.051
1.904SerLys: 1.904 ± 0.05
6.116SerLeu: 6.116 ± 0.084
1.533SerMet: 1.533 ± 0.035
1.621SerAsn: 1.621 ± 0.057
3.003SerPro: 3.003 ± 0.052
2.342SerGln: 2.342 ± 0.046
3.329SerArg: 3.329 ± 0.057
3.478SerSer: 3.478 ± 0.078
3.519SerThr: 3.519 ± 0.125
4.27SerVal: 4.27 ± 0.063
0.84SerTrp: 0.84 ± 0.024
1.38SerTyr: 1.38 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
5.992ThrAla: 5.992 ± 0.189
0.467ThrCys: 0.467 ± 0.022
2.798ThrAsp: 2.798 ± 0.111
2.45ThrGlu: 2.45 ± 0.043
1.768ThrPhe: 1.768 ± 0.042
4.727ThrGly: 4.727 ± 0.137
1.28ThrHis: 1.28 ± 0.032
2.25ThrIle: 2.25 ± 0.064
1.587ThrLys: 1.587 ± 0.039
6.455ThrLeu: 6.455 ± 0.096
1.258ThrMet: 1.258 ± 0.035
1.383ThrAsn: 1.383 ± 0.038
3.655ThrPro: 3.655 ± 0.064
2.337ThrGln: 2.337 ± 0.059
3.095ThrArg: 3.095 ± 0.056
3.13ThrSer: 3.13 ± 0.08
3.107ThrThr: 3.107 ± 0.133
4.438ThrVal: 4.438 ± 0.082
0.793ThrTrp: 0.793 ± 0.026
1.275ThrTyr: 1.275 ± 0.079
0.0ThrXaa: 0.0 ± 0.0
Val
8.837ValAla: 8.837 ± 0.098
0.793ValCys: 0.793 ± 0.03
4.268ValAsp: 4.268 ± 0.061
3.88ValGlu: 3.88 ± 0.068
2.65ValPhe: 2.65 ± 0.05
5.542ValGly: 5.542 ± 0.073
1.749ValHis: 1.749 ± 0.047
3.632ValIle: 3.632 ± 0.056
2.792ValLys: 2.792 ± 0.058
8.218ValLeu: 8.218 ± 0.107
2.173ValMet: 2.173 ± 0.046
2.401ValAsn: 2.401 ± 0.055
3.872ValPro: 3.872 ± 0.066
3.053ValGln: 3.053 ± 0.054
4.739ValArg: 4.739 ± 0.08
4.712ValSer: 4.712 ± 0.071
4.11ValThr: 4.11 ± 0.098
6.229ValVal: 6.229 ± 0.089
1.087ValTrp: 1.087 ± 0.028
1.575ValTyr: 1.575 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
1.65TrpAla: 1.65 ± 0.048
0.18TrpCys: 0.18 ± 0.013
0.651TrpAsp: 0.651 ± 0.024
0.493TrpGlu: 0.493 ± 0.018
0.525TrpPhe: 0.525 ± 0.02
1.094TrpGly: 1.094 ± 0.034
0.393TrpHis: 0.393 ± 0.015
0.576TrpIle: 0.576 ± 0.021
0.448TrpLys: 0.448 ± 0.02
2.25TrpLeu: 2.25 ± 0.05
0.475TrpMet: 0.475 ± 0.022
0.369TrpAsn: 0.369 ± 0.02
0.785TrpPro: 0.785 ± 0.029
0.908TrpGln: 0.908 ± 0.032
1.346TrpArg: 1.346 ± 0.042
0.852TrpSer: 0.852 ± 0.029
0.674TrpThr: 0.674 ± 0.024
1.114TrpVal: 1.114 ± 0.031
0.326TrpTrp: 0.326 ± 0.018
0.296TrpTyr: 0.296 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.514TyrAla: 2.514 ± 0.05
0.244TyrCys: 0.244 ± 0.014
1.211TyrAsp: 1.211 ± 0.036
1.171TyrGlu: 1.171 ± 0.037
0.935TyrPhe: 0.935 ± 0.03
1.976TyrGly: 1.976 ± 0.045
0.462TyrHis: 0.462 ± 0.033
0.882TyrIle: 0.882 ± 0.03
0.826TyrLys: 0.826 ± 0.029
2.453TyrLeu: 2.453 ± 0.044
0.48TyrMet: 0.48 ± 0.02
0.701TyrAsn: 0.701 ± 0.027
1.072TyrPro: 1.072 ± 0.031
1.005TyrGln: 1.005 ± 0.032
1.558TyrArg: 1.558 ± 0.037
1.169TyrSer: 1.169 ± 0.035
1.278TyrThr: 1.278 ± 0.048
1.692TyrVal: 1.692 ± 0.044
0.402TyrTrp: 0.402 ± 0.017
0.599TyrTyr: 0.599 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3620 proteins (1215807 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski