Grifola frondosa (Maitake) (Polyporus frondosus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Grifolaceae; Grifola

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14985 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C7LZH1|A0A1C7LZH1_GRIFR Uncharacterized protein OS=Grifola frondosa OX=5627 GN=A0H81_09933 PE=4 SV=1
MM1 pKa = 7.43AHH3 pKa = 7.42LFYY6 pKa = 9.96KK7 pKa = 9.28TRR9 pKa = 11.84LYY11 pKa = 10.23IVSPVHH17 pKa = 6.39QVPLSNNTVSDD28 pKa = 3.77AGDD31 pKa = 3.51GTEE34 pKa = 4.77SSNSTDD40 pKa = 3.18SVSSITSSTLSAINSTLVATVTDD63 pKa = 4.95SILSSATASVNSTTSTTAFSANATDD88 pKa = 3.99SSITATATDD97 pKa = 4.19PSVSASITDD106 pKa = 3.14SSDD109 pKa = 3.35PYY111 pKa = 11.25SSLPTVTNTDD121 pKa = 3.37SQTSTDD127 pKa = 3.46TSILSSTDD135 pKa = 2.75AAAPTDD141 pKa = 3.99APVSTGMPVSPPACVAKK158 pKa = 10.48AAGVAAGTTSSDD170 pKa = 3.22DD171 pKa = 3.52STPTDD176 pKa = 3.41VSSTIYY182 pKa = 10.67LSTSVFTTLSSQSTASTTSTVIPSTASSVTVTPTVTTSVSSALRR226 pKa = 11.84TDD228 pKa = 4.36GSNSPIPTNATISKK242 pKa = 10.09RR243 pKa = 11.84IAQADD248 pKa = 3.82LRR250 pKa = 11.84AVAQAWQDD258 pKa = 3.35LCLVSGGDD266 pKa = 2.91IFTNEE271 pKa = 4.2PCVQLAGINGINALLADD288 pKa = 5.37ADD290 pKa = 4.02PCAQQDD296 pKa = 3.75NADD299 pKa = 3.63AMIDD303 pKa = 3.8FAKK306 pKa = 10.81SPGVTNTDD314 pKa = 3.03ALVANAIAYY323 pKa = 7.53RR324 pKa = 11.84QHH326 pKa = 6.4PRR328 pKa = 11.84NAININGIVPSTPFCEE344 pKa = 3.88NAPRR348 pKa = 11.84NQEE351 pKa = 3.57LVGIVNAQLQGVDD364 pKa = 4.02FGIFGSVQFGLVAFGANGTCPFGQTADD391 pKa = 4.06IDD393 pKa = 4.08TCSCSS398 pKa = 3.08

Molecular weight: 40.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C7MNS1|A0A1C7MNS1_GRIFR Uncharacterized protein OS=Grifola frondosa OX=5627 GN=A0H81_02269 PE=4 SV=1
MM1 pKa = 7.85LSRR4 pKa = 11.84LPSRR8 pKa = 11.84NLRR11 pKa = 11.84PPPKK15 pKa = 9.65QKK17 pKa = 10.27EE18 pKa = 4.25SRR20 pKa = 11.84PSRR23 pKa = 11.84RR24 pKa = 11.84ARR26 pKa = 11.84VRR28 pKa = 11.84AARR31 pKa = 11.84SPPPKK36 pKa = 9.05PRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84ATHH44 pKa = 4.83PTLRR48 pKa = 11.84RR49 pKa = 11.84SRR51 pKa = 11.84TSWVGPAKK59 pKa = 10.48GRR61 pKa = 11.84AKK63 pKa = 10.49RR64 pKa = 11.84NNRR67 pKa = 11.84NAGG70 pKa = 3.29

Molecular weight: 8.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14985

0

14985

5484969

17

4995

366.0

40.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.839 ± 0.017

1.47 ± 0.009

5.559 ± 0.014

5.715 ± 0.019

3.705 ± 0.013

6.216 ± 0.017

2.776 ± 0.011

4.823 ± 0.015

4.143 ± 0.018

9.245 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.187 ± 0.007

3.198 ± 0.011

6.56 ± 0.024

3.604 ± 0.012

6.705 ± 0.019

8.814 ± 0.026

5.974 ± 0.014

6.433 ± 0.014

1.43 ± 0.007

2.606 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski