Jeotgalibaca dankookensis
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2003 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S6INU5|A0A1S6INU5_9LACT Elp3 domain-containing protein OS=Jeotgalibaca dankookensis OX=708126 GN=BW727_100818 PE=4 SV=1
MM1 pKa = 7.52 KK2 pKa = 9.19 KK3 pKa = 8.39 TVAILSSVFLLAACQDD19 pKa = 3.83 TTDD22 pKa = 3.48 ATQEE26 pKa = 4.18 SVVNEE31 pKa = 4.16 EE32 pKa = 3.98 SGQTQVSTVEE42 pKa = 4.35 SVEE45 pKa = 4.21 SEE47 pKa = 4.14 AVSSEE52 pKa = 3.97 VQEE55 pKa = 4.36 DD56 pKa = 3.67 VAYY59 pKa = 9.76 EE60 pKa = 3.94 YY61 pKa = 10.6 EE62 pKa = 4.41 INPDD66 pKa = 3.7 LYY68 pKa = 10.46 TVVPIDD74 pKa = 4.05 DD75 pKa = 4.77 ADD77 pKa = 4.1 TEE79 pKa = 4.76 VVLLTFDD86 pKa = 5.9 DD87 pKa = 5.19 APQPPNSYY95 pKa = 8.39 TLDD98 pKa = 3.31 IAQTVQDD105 pKa = 4.23 KK106 pKa = 10.31 GANAIFFVMGQFLEE120 pKa = 4.31 EE121 pKa = 4.37 PEE123 pKa = 3.98 AKK125 pKa = 10.26 EE126 pKa = 4.07 IIKK129 pKa = 8.96 TVYY132 pKa = 11.21 DD133 pKa = 3.33 MGFEE137 pKa = 4.26 IGNHH141 pKa = 5.87 SYY143 pKa = 10.76 SHH145 pKa = 7.61 PDD147 pKa = 3.22 LTTLTYY153 pKa = 10.56 EE154 pKa = 4.09 EE155 pKa = 4.18 QLAEE159 pKa = 3.91 ITKK162 pKa = 9.97 TSDD165 pKa = 2.99 LVEE168 pKa = 4.96 EE169 pKa = 4.48 ITGEE173 pKa = 3.8 RR174 pKa = 11.84 PRR176 pKa = 11.84 FIRR179 pKa = 11.84 APYY182 pKa = 9.99 GSYY185 pKa = 10.77 NSEE188 pKa = 3.9 TEE190 pKa = 4.38 AIADD194 pKa = 4.07 GEE196 pKa = 4.44 NMTIMNWTYY205 pKa = 10.84 GYY207 pKa = 10.82 DD208 pKa = 3.22 WVDD211 pKa = 3.34 GYY213 pKa = 10.19 MEE215 pKa = 4.79 EE216 pKa = 5.3 DD217 pKa = 4.19 AIADD221 pKa = 3.4 IMINAPEE228 pKa = 4.72 LGSGGNLLMHH238 pKa = 6.89 DD239 pKa = 4.28 RR240 pKa = 11.84 QWTSAAISRR249 pKa = 11.84 IIDD252 pKa = 3.46 GLRR255 pKa = 11.84 DD256 pKa = 3.22 AGYY259 pKa = 11.24 EE260 pKa = 4.12 MVDD263 pKa = 3.4 PTLIASPEE271 pKa = 3.98 EE272 pKa = 4.01 AA273 pKa = 4.22
Molecular weight: 30.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.745
IPC_protein 3.732
Toseland 3.541
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.605
Rodwell 3.567
Grimsley 3.439
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 3.986
Thurlkill 3.567
EMBOSS 3.617
Sillero 3.846
Patrickios 1.176
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.774
Protein with the highest isoelectric point:
>tr|A0A1S6IRL2|A0A1S6IRL2_9LACT Flagellar basal body rod protein FlgB OS=Jeotgalibaca dankookensis OX=708126 GN=flgB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPKK8 pKa = 8.11 KK9 pKa = 8.92 RR10 pKa = 11.84 KK11 pKa = 7.58 RR12 pKa = 11.84 QRR14 pKa = 11.84 VHH16 pKa = 6.34 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 10.02 NGRR28 pKa = 11.84 NVLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.521
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.749
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.486
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2003
0
2003
623611
29
3192
311.3
34.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.965 ± 0.058
0.49 ± 0.016
5.471 ± 0.045
7.623 ± 0.059
4.461 ± 0.045
6.564 ± 0.048
1.818 ± 0.026
8.082 ± 0.058
6.586 ± 0.043
9.795 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.766 ± 0.027
4.742 ± 0.038
3.429 ± 0.026
3.92 ± 0.034
4.037 ± 0.038
6.077 ± 0.04
5.766 ± 0.035
6.844 ± 0.04
0.834 ± 0.018
3.73 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here