Amino acid dipepetide frequency for bacterium AM6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.327AlaAla: 18.327 ± 0.247	1.352AlaCys: 1.352 ± 0.046	7.324AlaAsp: 7.324 ± 0.127	6.744AlaGlu: 6.744 ± 0.103	4.079AlaPhe: 4.079 ± 0.086	11.199AlaGly: 11.199 ± 0.15	2.462AlaHis: 2.462 ± 0.079	4.887AlaIle: 4.887 ± 0.094	3.49AlaLys: 3.49 ± 0.099	15.279AlaLeu: 15.279 ± 0.218	3.377AlaMet: 3.377 ± 0.08	2.921AlaAsn: 2.921 ± 0.078	6.311AlaPro: 6.311 ± 0.123	5.766AlaGln: 5.766 ± 0.12	9.016AlaArg: 9.016 ± 0.145	6.914AlaSer: 6.914 ± 0.113	6.228AlaThr: 6.228 ± 0.118	8.81AlaVal: 8.81 ± 0.121	2.0AlaTrp: 2.0 ± 0.068	2.375AlaTyr: 2.375 ± 0.073	0.0AlaXaa: 0.0 ± 0.0
Cys	1.061CysAla: 1.061 ± 0.047	0.148CysCys: 0.148 ± 0.017	0.452CysAsp: 0.452 ± 0.024	0.431CysGlu: 0.431 ± 0.026	0.264CysPhe: 0.264 ± 0.024	0.923CysGly: 0.923 ± 0.044	0.261CysHis: 0.261 ± 0.025	0.336CysIle: 0.336 ± 0.028	0.2CysLys: 0.2 ± 0.02	0.875CysLeu: 0.875 ± 0.038	0.176CysMet: 0.176 ± 0.018	0.263CysAsn: 0.263 ± 0.023	0.446CysPro: 0.446 ± 0.03	0.356CysGln: 0.356 ± 0.025	0.725CysArg: 0.725 ± 0.04	0.609CysSer: 0.609 ± 0.031	0.577CysThr: 0.577 ± 0.031	0.667CysVal: 0.667 ± 0.036	0.155CysTrp: 0.155 ± 0.017	0.151CysTyr: 0.151 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	8.259AspAla: 8.259 ± 0.134	0.434AspCys: 0.434 ± 0.031	3.179AspAsp: 3.179 ± 0.087	3.097AspGlu: 3.097 ± 0.082	2.056AspPhe: 2.056 ± 0.069	5.681AspGly: 5.681 ± 0.112	1.364AspHis: 1.364 ± 0.057	2.344AspIle: 2.344 ± 0.075	1.574AspLys: 1.574 ± 0.059	5.48AspLeu: 5.48 ± 0.09	1.04AspMet: 1.04 ± 0.044	1.472AspAsn: 1.472 ± 0.058	3.297AspPro: 3.297 ± 0.077	1.845AspGln: 1.845 ± 0.057	3.76AspArg: 3.76 ± 0.084	2.695AspSer: 2.695 ± 0.07	2.667AspThr: 2.667 ± 0.072	4.119AspVal: 4.119 ± 0.092	0.998AspTrp: 0.998 ± 0.047	1.69AspTyr: 1.69 ± 0.059	0.0AspXaa: 0.0 ± 0.0
Glu	6.414GluAla: 6.414 ± 0.122	0.338GluCys: 0.338 ± 0.026	2.548GluAsp: 2.548 ± 0.068	2.405GluGlu: 2.405 ± 0.089	1.564GluPhe: 1.564 ± 0.059	3.977GluGly: 3.977 ± 0.086	1.357GluHis: 1.357 ± 0.046	2.229GluIle: 2.229 ± 0.067	1.57GluLys: 1.57 ± 0.063	5.844GluLeu: 5.844 ± 0.101	1.085GluMet: 1.085 ± 0.039	1.194GluAsn: 1.194 ± 0.052	2.292GluPro: 2.292 ± 0.057	2.966GluGln: 2.966 ± 0.077	4.899GluArg: 4.899 ± 0.092	2.259GluSer: 2.259 ± 0.064	2.304GluThr: 2.304 ± 0.066	3.66GluVal: 3.66 ± 0.079	0.652GluTrp: 0.652 ± 0.035	1.001GluTyr: 1.001 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	4.137PheAla: 4.137 ± 0.093	0.324PheCys: 0.324 ± 0.024	2.542PheAsp: 2.542 ± 0.066	1.803PheGlu: 1.803 ± 0.059	1.186PhePhe: 1.186 ± 0.053	3.31PheGly: 3.31 ± 0.075	0.72PheHis: 0.72 ± 0.037	1.289PheIle: 1.289 ± 0.055	0.938PheLys: 0.938 ± 0.047	3.044PheLeu: 3.044 ± 0.074	0.64PheMet: 0.64 ± 0.03	1.156PheAsn: 1.156 ± 0.043	1.416PhePro: 1.416 ± 0.044	1.046PheGln: 1.046 ± 0.04	1.941PheArg: 1.941 ± 0.063	2.043PheSer: 2.043 ± 0.062	1.705PheThr: 1.705 ± 0.059	2.613PheVal: 2.613 ± 0.067	0.517PheTrp: 0.517 ± 0.031	0.795PheTyr: 0.795 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	8.679GlyAla: 8.679 ± 0.143	0.94GlyCys: 0.94 ± 0.044	4.679GlyAsp: 4.679 ± 0.093	4.573GlyGlu: 4.573 ± 0.079	3.285GlyPhe: 3.285 ± 0.076	7.092GlyGly: 7.092 ± 0.157	2.101GlyHis: 2.101 ± 0.058	3.818GlyIle: 3.818 ± 0.093	3.131GlyLys: 3.131 ± 0.084	8.868GlyLeu: 8.868 ± 0.14	2.357GlyMet: 2.357 ± 0.061	2.738GlyAsn: 2.738 ± 0.09	3.061GlyPro: 3.061 ± 0.08	3.879GlyGln: 3.879 ± 0.09	5.955GlyArg: 5.955 ± 0.113	5.065GlySer: 5.065 ± 0.111	4.618GlyThr: 4.618 ± 0.113	6.177GlyVal: 6.177 ± 0.1	1.695GlyTrp: 1.695 ± 0.061	2.269GlyTyr: 2.269 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	3.089HisAla: 3.089 ± 0.087	0.278HisCys: 0.278 ± 0.028	1.394HisAsp: 1.394 ± 0.055	1.058HisGlu: 1.058 ± 0.044	0.855HisPhe: 0.855 ± 0.037	2.387HisGly: 2.387 ± 0.067	0.63HisHis: 0.63 ± 0.037	0.719HisIle: 0.719 ± 0.038	0.451HisLys: 0.451 ± 0.029	2.227HisLeu: 2.227 ± 0.072	0.484HisMet: 0.484 ± 0.031	0.562HisAsn: 0.562 ± 0.031	1.416HisPro: 1.416 ± 0.05	0.717HisGln: 0.717 ± 0.033	1.703HisArg: 1.703 ± 0.064	1.03HisSer: 1.03 ± 0.04	1.026HisThr: 1.026 ± 0.044	1.61HisVal: 1.61 ± 0.051	0.464HisTrp: 0.464 ± 0.03	0.684HisTyr: 0.684 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.974IleAla: 5.974 ± 0.11	0.403IleCys: 0.403 ± 0.028	3.247IleAsp: 3.247 ± 0.076	2.726IleGlu: 2.726 ± 0.082	0.981IlePhe: 0.981 ± 0.042	4.315IleGly: 4.315 ± 0.101	0.81IleHis: 0.81 ± 0.038	1.481IleIle: 1.481 ± 0.063	1.131IleLys: 1.131 ± 0.041	3.109IleLeu: 3.109 ± 0.078	0.551IleMet: 0.551 ± 0.033	1.327IleAsn: 1.327 ± 0.053	1.908IlePro: 1.908 ± 0.056	1.135IleGln: 1.135 ± 0.043	2.409IleArg: 2.409 ± 0.067	2.366IleSer: 2.366 ± 0.064	2.272IleThr: 2.272 ± 0.064	3.016IleVal: 3.016 ± 0.078	0.459IleTrp: 0.459 ± 0.028	0.805IleTyr: 0.805 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	3.675LysAla: 3.675 ± 0.095	0.148LysCys: 0.148 ± 0.018	1.507LysAsp: 1.507 ± 0.059	1.214LysGlu: 1.214 ± 0.053	0.657LysPhe: 0.657 ± 0.039	2.301LysGly: 2.301 ± 0.07	0.625LysHis: 0.625 ± 0.032	1.171LysIle: 1.171 ± 0.047	1.116LysLys: 1.116 ± 0.09	2.976LysLeu: 2.976 ± 0.075	0.695LysMet: 0.695 ± 0.039	0.75LysAsn: 0.75 ± 0.039	1.823LysPro: 1.823 ± 0.061	1.289LysGln: 1.289 ± 0.048	2.171LysArg: 2.171 ± 0.06	1.308LysSer: 1.308 ± 0.055	1.489LysThr: 1.489 ± 0.055	2.395LysVal: 2.395 ± 0.078	0.284LysTrp: 0.284 ± 0.025	0.586LysTyr: 0.586 ± 0.033	0.0LysXaa: 0.0 ± 0.0
Leu	14.907LeuAla: 14.907 ± 0.194	1.05LeuCys: 1.05 ± 0.046	6.474LeuAsp: 6.474 ± 0.1	4.956LeuGlu: 4.956 ± 0.097	3.438LeuPhe: 3.438 ± 0.081	8.792LeuGly: 8.792 ± 0.148	2.613LeuHis: 2.613 ± 0.07	3.954LeuIle: 3.954 ± 0.089	3.111LeuLys: 3.111 ± 0.08	13.049LeuLeu: 13.049 ± 0.225	2.164LeuMet: 2.164 ± 0.058	2.465LeuAsn: 2.465 ± 0.073	6.877LeuPro: 6.877 ± 0.129	5.536LeuGln: 5.536 ± 0.121	8.451LeuArg: 8.451 ± 0.143	5.937LeuSer: 5.937 ± 0.109	4.758LeuThr: 4.758 ± 0.101	7.9LeuVal: 7.9 ± 0.116	1.466LeuTrp: 1.466 ± 0.058	2.124LeuTyr: 2.124 ± 0.068	0.0LeuXaa: 0.0 ± 0.0
Met	2.89MetAla: 2.89 ± 0.082	0.125MetCys: 0.125 ± 0.013	1.281MetAsp: 1.281 ± 0.047	0.945MetGlu: 0.945 ± 0.044	0.66MetPhe: 0.66 ± 0.039	1.54MetGly: 1.54 ± 0.058	0.492MetHis: 0.492 ± 0.027	0.935MetIle: 0.935 ± 0.044	0.885MetLys: 0.885 ± 0.041	2.42MetLeu: 2.42 ± 0.074	0.486MetMet: 0.486 ± 0.033	0.795MetAsn: 0.795 ± 0.036	1.436MetPro: 1.436 ± 0.047	1.043MetGln: 1.043 ± 0.04	1.642MetArg: 1.642 ± 0.047	1.624MetSer: 1.624 ± 0.051	1.424MetThr: 1.424 ± 0.043	1.486MetVal: 1.486 ± 0.056	0.238MetTrp: 0.238 ± 0.02	0.333MetTyr: 0.333 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	3.38AsnAla: 3.38 ± 0.081	0.203AsnCys: 0.203 ± 0.02	1.489AsnAsp: 1.489 ± 0.052	1.224AsnGlu: 1.224 ± 0.049	0.903AsnPhe: 0.903 ± 0.039	2.635AsnGly: 2.635 ± 0.085	0.592AsnHis: 0.592 ± 0.031	1.083AsnIle: 1.083 ± 0.043	0.715AsnLys: 0.715 ± 0.031	2.563AsnLeu: 2.563 ± 0.071	0.411AsnMet: 0.411 ± 0.029	0.732AsnAsn: 0.732 ± 0.044	1.723AsnPro: 1.723 ± 0.06	0.897AsnGln: 0.897 ± 0.043	1.743AsnArg: 1.743 ± 0.058	1.214AsnSer: 1.214 ± 0.046	1.397AsnThr: 1.397 ± 0.053	2.023AsnVal: 2.023 ± 0.065	0.428AsnTrp: 0.428 ± 0.027	0.684AsnTyr: 0.684 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	7.546ProAla: 7.546 ± 0.141	0.319ProCys: 0.319 ± 0.024	3.157ProAsp: 3.157 ± 0.072	2.973ProGlu: 2.973 ± 0.082	1.66ProPhe: 1.66 ± 0.049	4.526ProGly: 4.526 ± 0.088	1.061ProHis: 1.061 ± 0.043	1.837ProIle: 1.837 ± 0.057	1.394ProLys: 1.394 ± 0.051	5.584ProLeu: 5.584 ± 0.103	1.374ProMet: 1.374 ± 0.043	1.131ProAsn: 1.131 ± 0.045	2.652ProPro: 2.652 ± 0.086	2.319ProGln: 2.319 ± 0.07	3.399ProArg: 3.399 ± 0.069	2.899ProSer: 2.899 ± 0.08	2.415ProThr: 2.415 ± 0.07	4.4ProVal: 4.4 ± 0.084	0.878ProTrp: 0.878 ± 0.036	1.116ProTyr: 1.116 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	6.336GlnAla: 6.336 ± 0.109	0.353GlnCys: 0.353 ± 0.023	1.916GlnAsp: 1.916 ± 0.06	1.644GlnGlu: 1.644 ± 0.054	1.266GlnPhe: 1.266 ± 0.045	3.359GlnGly: 3.359 ± 0.084	1.07GlnHis: 1.07 ± 0.039	1.672GlnIle: 1.672 ± 0.05	1.061GlnLys: 1.061 ± 0.047	4.857GlnLeu: 4.857 ± 0.103	0.98GlnMet: 0.98 ± 0.04	0.792GlnAsn: 0.792 ± 0.038	2.402GlnPro: 2.402 ± 0.074	2.712GlnGln: 2.712 ± 0.089	4.307GlnArg: 4.307 ± 0.096	1.812GlnSer: 1.812 ± 0.056	1.878GlnThr: 1.878 ± 0.059	3.678GlnVal: 3.678 ± 0.086	0.817GlnTrp: 0.817 ± 0.033	0.858GlnTyr: 0.858 ± 0.044	0.0GlnXaa: 0.0 ± 0.0
Arg	7.73ArgAla: 7.73 ± 0.114	0.712ArgCys: 0.712 ± 0.035	4.23ArgAsp: 4.23 ± 0.085	4.239ArgGlu: 4.239 ± 0.094	2.878ArgPhe: 2.878 ± 0.065	5.059ArgGly: 5.059 ± 0.092	1.943ArgHis: 1.943 ± 0.06	3.736ArgIle: 3.736 ± 0.082	1.943ArgLys: 1.943 ± 0.06	8.093ArgLeu: 8.093 ± 0.139	2.131ArgMet: 2.131 ± 0.061	2.016ArgAsn: 2.016 ± 0.056	3.352ArgPro: 3.352 ± 0.087	3.475ArgGln: 3.475 ± 0.073	6.057ArgArg: 6.057 ± 0.122	4.037ArgSer: 4.037 ± 0.089	3.304ArgThr: 3.304 ± 0.071	5.17ArgVal: 5.17 ± 0.094	1.535ArgTrp: 1.535 ± 0.057	2.126ArgTyr: 2.126 ± 0.065	0.0ArgXaa: 0.0 ± 0.0
Ser	6.774SerAla: 6.774 ± 0.124	0.449SerCys: 0.449 ± 0.03	2.693SerAsp: 2.693 ± 0.064	2.632SerGlu: 2.632 ± 0.066	1.818SerPhe: 1.818 ± 0.06	5.162SerGly: 5.162 ± 0.115	1.191SerHis: 1.191 ± 0.044	2.234SerIle: 2.234 ± 0.06	1.444SerLys: 1.444 ± 0.06	5.829SerLeu: 5.829 ± 0.1	1.193SerMet: 1.193 ± 0.05	1.544SerAsn: 1.544 ± 0.05	2.908SerPro: 2.908 ± 0.08	1.968SerGln: 1.968 ± 0.06	3.774SerArg: 3.774 ± 0.086	3.297SerSer: 3.297 ± 0.095	2.959SerThr: 2.959 ± 0.07	3.846SerVal: 3.846 ± 0.087	0.885SerTrp: 0.885 ± 0.041	1.317SerTyr: 1.317 ± 0.059	0.0SerXaa: 0.0 ± 0.0
Thr	6.132ThrAla: 6.132 ± 0.141	0.442ThrCys: 0.442 ± 0.026	2.532ThrAsp: 2.532 ± 0.069	2.144ThrGlu: 2.144 ± 0.06	1.659ThrPhe: 1.659 ± 0.056	4.495ThrGly: 4.495 ± 0.097	1.075ThrHis: 1.075 ± 0.044	1.898ThrIle: 1.898 ± 0.069	0.963ThrLys: 0.963 ± 0.043	6.421ThrLeu: 6.421 ± 0.109	0.957ThrMet: 0.957 ± 0.036	1.048ThrAsn: 1.048 ± 0.051	3.374ThrPro: 3.374 ± 0.083	1.69ThrGln: 1.69 ± 0.056	3.314ThrArg: 3.314 ± 0.079	2.553ThrSer: 2.553 ± 0.069	2.668ThrThr: 2.668 ± 0.061	4.187ThrVal: 4.187 ± 0.099	0.709ThrTrp: 0.709 ± 0.032	1.038ThrTyr: 1.038 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	9.452ValAla: 9.452 ± 0.136	0.652ValCys: 0.652 ± 0.036	4.48ValAsp: 4.48 ± 0.088	4.154ValGlu: 4.154 ± 0.076	2.57ValPhe: 2.57 ± 0.064	5.426ValGly: 5.426 ± 0.094	1.592ValHis: 1.592 ± 0.055	3.357ValIle: 3.357 ± 0.076	1.963ValLys: 1.963 ± 0.065	8.665ValLeu: 8.665 ± 0.139	1.692ValMet: 1.692 ± 0.054	2.011ValAsn: 2.011 ± 0.065	3.941ValPro: 3.941 ± 0.087	3.164ValGln: 3.164 ± 0.073	5.2ValArg: 5.2 ± 0.093	4.062ValSer: 4.062 ± 0.084	3.626ValThr: 3.626 ± 0.092	6.15ValVal: 6.15 ± 0.113	0.908ValTrp: 0.908 ± 0.044	1.509ValTyr: 1.509 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	1.461TrpAla: 1.461 ± 0.062	0.176TrpCys: 0.176 ± 0.018	0.62TrpAsp: 0.62 ± 0.035	0.537TrpGlu: 0.537 ± 0.032	0.561TrpPhe: 0.561 ± 0.032	0.947TrpGly: 0.947 ± 0.041	0.371TrpHis: 0.371 ± 0.025	0.687TrpIle: 0.687 ± 0.043	0.534TrpLys: 0.534 ± 0.033	2.271TrpLeu: 2.271 ± 0.08	0.536TrpMet: 0.536 ± 0.031	0.502TrpAsn: 0.502 ± 0.032	0.855TrpPro: 0.855 ± 0.041	0.986TrpGln: 0.986 ± 0.043	1.376TrpArg: 1.376 ± 0.053	0.873TrpSer: 0.873 ± 0.046	0.769TrpThr: 0.769 ± 0.035	1.051TrpVal: 1.051 ± 0.047	0.339TrpTrp: 0.339 ± 0.026	0.346TrpTyr: 0.346 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.64TyrAla: 2.64 ± 0.072	0.231TyrCys: 0.231 ± 0.019	1.303TyrAsp: 1.303 ± 0.05	1.061TyrGlu: 1.061 ± 0.05	0.827TyrPhe: 0.827 ± 0.038	1.856TyrGly: 1.856 ± 0.06	0.452TyrHis: 0.452 ± 0.028	0.759TyrIle: 0.759 ± 0.031	0.567TyrLys: 0.567 ± 0.032	2.565TyrLeu: 2.565 ± 0.07	0.349TyrMet: 0.349 ± 0.026	0.619TyrAsn: 0.619 ± 0.032	1.153TyrPro: 1.153 ± 0.045	0.98TyrGln: 0.98 ± 0.04	1.925TyrArg: 1.925 ± 0.058	1.321TyrSer: 1.321 ± 0.053	1.213TyrThr: 1.213 ± 0.055	1.617TyrVal: 1.617 ± 0.052	0.371TyrTrp: 0.371 ± 0.027	0.596TyrTyr: 0.596 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2443 proteins (601141 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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