Sanxia water strider virus 20
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KF97|A0A1L3KF97_9VIRU RNA-directed RNA polymerase OS=Sanxia water strider virus 20 OX=1923404 PE=4 SV=1
MM1 pKa = 8.0 ADD3 pKa = 3.78 PHH5 pKa = 6.71 PLPPDD10 pKa = 3.44 LRR12 pKa = 11.84 SKK14 pKa = 10.1 GTVLRR19 pKa = 11.84 EE20 pKa = 3.64 EE21 pKa = 4.61 TSCHH25 pKa = 5.58 NGKK28 pKa = 7.64 PTPAQQKK35 pKa = 7.41 TVNQLNSLITNVVAAPSSPSVSTSPKK61 pKa = 9.49 ASPVPGPLPASRR73 pKa = 11.84 PVPQVYY79 pKa = 9.63 IPKK82 pKa = 8.02 TTQPRR87 pKa = 11.84 PMTTADD93 pKa = 3.33 YY94 pKa = 10.47 MNFEE98 pKa = 4.28 TSITIDD104 pKa = 4.24 DD105 pKa = 5.02 LPPLLPRR112 pKa = 11.84 PPAGKK117 pKa = 10.04 EE118 pKa = 3.52 PAKK121 pKa = 10.55 QPEE124 pKa = 4.56 FVPAVPDD131 pKa = 4.3 FSKK134 pKa = 11.0 FSTEE138 pKa = 3.66 MSKK141 pKa = 9.93 MDD143 pKa = 3.04 IVQRR147 pKa = 11.84 KK148 pKa = 7.53 VFMAQKK154 pKa = 10.18 NKK156 pKa = 8.77 EE157 pKa = 4.25 HH158 pKa = 6.91 NDD160 pKa = 3.12 LLRR163 pKa = 11.84 IYY165 pKa = 8.9 NQQYY169 pKa = 8.69 AQYY172 pKa = 10.32 LAEE175 pKa = 5.06 HH176 pKa = 6.98 PNATDD181 pKa = 3.6 FRR183 pKa = 11.84 GRR185 pKa = 11.84 NPQPILPQGIPQPISSPALTPGLNSTLLAKK215 pKa = 10.35 DD216 pKa = 3.54 PPPRR220 pKa = 11.84 ASAGHH225 pKa = 6.73 ALYY228 pKa = 10.2 IPKK231 pKa = 10.29 DD232 pKa = 3.12 ILARR236 pKa = 11.84 EE237 pKa = 4.52 GSTTSGPSALTSIAPAAPEE256 pKa = 4.21 PMSDD260 pKa = 3.58 DD261 pKa = 3.88 KK262 pKa = 11.56 IFTGRR267 pKa = 11.84 VNEE270 pKa = 4.97 SITTLYY276 pKa = 10.73 KK277 pKa = 10.47 HH278 pKa = 6.12 VFTDD282 pKa = 5.98 GIHH285 pKa = 5.96 EE286 pKa = 4.25 RR287 pKa = 11.84 LNIQGCEE294 pKa = 3.95 DD295 pKa = 3.96 GATKK299 pKa = 10.48 LARR302 pKa = 11.84 AILLAVDD309 pKa = 3.79 SPVARR314 pKa = 11.84 DD315 pKa = 3.49 CGSRR319 pKa = 11.84 QQPDD323 pKa = 3.24 AAVDD327 pKa = 3.47 ALYY330 pKa = 11.55 AMAPTSADD338 pKa = 3.11 SYY340 pKa = 9.78 GTRR343 pKa = 11.84 TTYY346 pKa = 10.53 RR347 pKa = 11.84 RR348 pKa = 11.84 VDD350 pKa = 3.34 NGVDD354 pKa = 2.99 ANGRR358 pKa = 11.84 QKK360 pKa = 9.51 TRR362 pKa = 11.84 EE363 pKa = 3.9 VTEE366 pKa = 3.85 TRR368 pKa = 11.84 TVRR371 pKa = 11.84 KK372 pKa = 10.13 GSLTKK377 pKa = 10.48 SANDD381 pKa = 3.32 LAARR385 pKa = 11.84 LIDD388 pKa = 3.97 GNNNGYY394 pKa = 10.41 RR395 pKa = 11.84 IANFFSRR402 pKa = 11.84 SSSNRR407 pKa = 11.84 LFAVAVARR415 pKa = 11.84 ALLRR419 pKa = 11.84 KK420 pKa = 9.31 KK421 pKa = 10.49 ISVALYY427 pKa = 10.65 SKK429 pKa = 10.74 GLNSASKK436 pKa = 10.63 ARR438 pKa = 11.84 ALISAIQAYY447 pKa = 9.72 AADD450 pKa = 4.14 AVEE453 pKa = 4.62 TTSPTLDD460 pKa = 3.01 TSTRR464 pKa = 11.84 DD465 pKa = 3.24 IPDD468 pKa = 4.02 AEE470 pKa = 4.24 TLPRR474 pKa = 11.84 SILPEE479 pKa = 4.78 PIEE482 pKa = 4.41 SMSASLAYY490 pKa = 10.24 SRR492 pKa = 11.84 LIAAKK497 pKa = 10.45 LIDD500 pKa = 4.43 LRR502 pKa = 11.84 ACPVCVACAILGEE515 pKa = 4.27 DD516 pKa = 3.43 VPAEE520 pKa = 4.26 VEE522 pKa = 4.27 TPTATKK528 pKa = 10.48 QNLRR532 pKa = 11.84 EE533 pKa = 3.98 QPPTSISKK541 pKa = 10.31 LRR543 pKa = 11.84 TLLSNGNSSRR553 pKa = 11.84 FLEE556 pKa = 4.65 AVANAHH562 pKa = 5.09 NRR564 pKa = 11.84 EE565 pKa = 3.96 MHH567 pKa = 6.22 SLNGNISFIVGLEE580 pKa = 3.87 EE581 pKa = 4.69 PRR583 pKa = 11.84 PQVIQRR589 pKa = 11.84 CIIEE593 pKa = 4.53 TVQPPVKK600 pKa = 10.75 VPVTSFRR607 pKa = 11.84 DD608 pKa = 3.31 DD609 pKa = 3.62 AYY611 pKa = 11.48 NILQQKK617 pKa = 6.04 TTDD620 pKa = 3.49 EE621 pKa = 4.01 LTLRR625 pKa = 11.84 VGDD628 pKa = 3.77 GRR630 pKa = 11.84 STITLQPAPTLHH642 pKa = 6.37 QLVLNLDD649 pKa = 3.77 PAARR653 pKa = 11.84 GPMLDD658 pKa = 4.0 AGNNMTIPPRR668 pKa = 11.84 MRR670 pKa = 11.84 DD671 pKa = 3.72 NQPSTSSSDD680 pKa = 3.52 APPPAQAGKK689 pKa = 10.89 GKK691 pKa = 9.19 ATVRR695 pKa = 11.84 NASKK699 pKa = 9.55 TRR701 pKa = 11.84 AQTDD705 pKa = 3.35 KK706 pKa = 10.9 APEE709 pKa = 4.21 VFSEE713 pKa = 4.72 PPTQTSSSNSTPDD726 pKa = 3.25 PRR728 pKa = 11.84 KK729 pKa = 7.88 TSVLGRR735 pKa = 11.84 IRR737 pKa = 11.84 KK738 pKa = 7.44 VFKK741 pKa = 11.09 VIDD744 pKa = 4.1 KK745 pKa = 10.99 VPATCKK751 pKa = 9.34 TNRR754 pKa = 11.84 PSTILGDD761 pKa = 4.03 APDD764 pKa = 3.98 LSGLGTSSSLGDD776 pKa = 3.31 SDD778 pKa = 5.83 SFISYY783 pKa = 9.47 PDD785 pKa = 2.92 IMAAAGPAYY794 pKa = 10.19 VPPLYY799 pKa = 10.3 IGVGQSADD807 pKa = 3.22 VGGGHH812 pKa = 7.05 FFLNPVATDD821 pKa = 3.39 FLARR825 pKa = 11.84 VRR827 pKa = 11.84 DD828 pKa = 3.8 ADD830 pKa = 3.8 TKK832 pKa = 11.08 EE833 pKa = 3.75 PGIACRR839 pKa = 11.84 LPEE842 pKa = 4.75 NILGRR847 pKa = 11.84 LANAPYY853 pKa = 10.66 LLSALANTLPSDD865 pKa = 3.73 NSDD868 pKa = 3.18 FTARR872 pKa = 11.84 TLKK875 pKa = 10.22 VSLYY879 pKa = 9.82 EE880 pKa = 4.05 GLRR883 pKa = 11.84 EE884 pKa = 3.95 MRR886 pKa = 11.84 NTNPLGVAFAAPLNAYY902 pKa = 7.93 PIRR905 pKa = 11.84 EE906 pKa = 4.19 NIALNQQQDD915 pKa = 2.74 WQYY918 pKa = 9.06 RR919 pKa = 11.84 HH920 pKa = 6.67 IIVSYY925 pKa = 10.44 SYY927 pKa = 11.25 LEE929 pKa = 3.69 EE930 pKa = 4.33 TIRR933 pKa = 11.84 LGAEE937 pKa = 3.74 IPVAGQDD944 pKa = 3.37 PWSLKK949 pKa = 10.43 AQDD952 pKa = 3.9 TVVVCLDD959 pKa = 3.48 YY960 pKa = 11.48 DD961 pKa = 3.75 AVDD964 pKa = 4.0 TNVVWALQMLTALPYY979 pKa = 9.11 PLLWVAEE986 pKa = 4.05 WQAYY990 pKa = 8.46 LRR992 pKa = 11.84 QRR994 pKa = 11.84 NDD996 pKa = 3.15 DD997 pKa = 3.69 PTDD1000 pKa = 3.57 VFWTLGQFLRR1010 pKa = 11.84 NDD1012 pKa = 3.8 GLVSLNDD1019 pKa = 3.46 MVKK1022 pKa = 10.35 KK1023 pKa = 10.7 VIFVTSTMLPQITISGHH1040 pKa = 6.5 LYY1042 pKa = 9.7 PVTVSLGPRR1051 pKa = 11.84 VPLPPAQVYY1060 pKa = 10.48 DD1061 pKa = 3.93 SEE1063 pKa = 4.82 ALILSSLTLILASVTPLRR1081 pKa = 11.84 KK1082 pKa = 8.87 IWEE1085 pKa = 4.52 SLWSQRR1091 pKa = 11.84 LGGSALNWTRR1101 pKa = 11.84 IEE1103 pKa = 4.21 NYY1105 pKa = 9.33 VAYY1108 pKa = 8.86 LTTRR1112 pKa = 11.84 WHH1114 pKa = 6.59 PQLEE1118 pKa = 4.47 MATGGDD1124 pKa = 4.21 GVPHH1128 pKa = 5.99 SFWWQGPLPDD1138 pKa = 4.15 NSPILFEE1145 pKa = 4.76 MGDD1148 pKa = 3.86 ADD1150 pKa = 4.51 TLFATDD1156 pKa = 4.63 AVTAGDD1162 pKa = 4.3 NIFLPRR1168 pKa = 11.84 PDD1170 pKa = 4.9 ADD1172 pKa = 3.47 INPAMRR1178 pKa = 11.84 LGRR1181 pKa = 11.84 WTNWAEE1187 pKa = 3.79 LAVGLRR1193 pKa = 11.84 VASYY1197 pKa = 10.61 RR1198 pKa = 11.84 SEE1200 pKa = 4.03 SLNLQQLDD1208 pKa = 3.63 PHH1210 pKa = 7.65 IDD1212 pKa = 3.09 SSGYY1216 pKa = 10.5 SRR1218 pKa = 11.84 LTVAKK1223 pKa = 8.49 FQRR1226 pKa = 11.84 RR1227 pKa = 11.84 AIEE1230 pKa = 3.78 EE1231 pKa = 4.13 WKK1233 pKa = 10.73 YY1234 pKa = 11.55 SSLTSDD1240 pKa = 4.35 EE1241 pKa = 3.98 IVAPHH1246 pKa = 7.4 AYY1248 pKa = 10.25 NLLGGNWNKK1257 pKa = 10.08 LVYY1260 pKa = 10.69 GDD1262 pKa = 4.93 GSTKK1266 pKa = 10.25 QLFFSLLVARR1276 pKa = 11.84 ILGLTPSDD1284 pKa = 3.41 GTMNWSGNWMRR1295 pKa = 11.84 AVNQTFTGIRR1305 pKa = 11.84 TSNAMWLDD1313 pKa = 3.57 EE1314 pKa = 4.2 RR1315 pKa = 11.84 SGDD1318 pKa = 3.85 LTCTITDD1325 pKa = 3.36 TSLTYY1330 pKa = 9.18 ITNLNDD1336 pKa = 3.64 PLYY1339 pKa = 9.91 QFWVQAQNPKK1349 pKa = 10.93 DD1350 pKa = 3.95 DD1351 pKa = 3.87 VTLSRR1356 pKa = 11.84 LLNRR1360 pKa = 11.84 LDD1362 pKa = 5.11 LLQQHH1367 pKa = 6.23 TALQSKK1373 pKa = 8.12 VWPQAALLPINMVRR1387 pKa = 11.84 LEE1389 pKa = 4.38 TTTFTALNILAGNNLLLNHH1408 pKa = 6.77 PTTPLEE1414 pKa = 4.09 MPIGWSYY1421 pKa = 11.46 GSPILQKK1428 pKa = 10.77 DD1429 pKa = 3.11 WTQFRR1434 pKa = 11.84 FLSVAVSAAAQRR1446 pKa = 11.84 SLEE1449 pKa = 4.18 TRR1451 pKa = 11.84 TSSPGTTLRR1460 pKa = 11.84 WPGVLIKK1467 pKa = 10.64 SEE1469 pKa = 4.25 GSAATYY1475 pKa = 10.55 LEE1477 pKa = 4.63 HH1478 pKa = 7.96 DD1479 pKa = 3.59 WTLLADD1485 pKa = 4.03 EE1486 pKa = 4.46 VTAMGGTPAGVTSSSDD1502 pKa = 3.08 SSVFRR1507 pKa = 11.84 LGPSSTDD1514 pKa = 2.83 RR1515 pKa = 11.84 GPPPDD1520 pKa = 3.95 GVTSSGQEE1528 pKa = 3.76 NPMVQPPGSDD1538 pKa = 3.44 DD1539 pKa = 3.43 ARR1541 pKa = 11.84 LGAEE1545 pKa = 4.28 GLSNHH1550 pKa = 7.13 PDD1552 pKa = 3.33 HH1553 pKa = 6.85 NQDD1556 pKa = 4.03 RR1557 pKa = 11.84 PQIDD1561 pKa = 2.98
Molecular weight: 170.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.962
IPC2_protein 5.906
IPC_protein 5.97
Toseland 6.338
ProMoST 6.275
Dawson 6.237
Bjellqvist 6.249
Wikipedia 6.249
Rodwell 6.224
Grimsley 6.491
Solomon 6.237
Lehninger 6.237
Nozaki 6.504
DTASelect 6.707
Thurlkill 6.707
EMBOSS 6.664
Sillero 6.605
Patrickios 4.406
IPC_peptide 6.262
IPC2_peptide 6.547
IPC2.peptide.svr19 6.592
Protein with the highest isoelectric point:
>tr|A0A1L3KF97|A0A1L3KF97_9VIRU RNA-directed RNA polymerase OS=Sanxia water strider virus 20 OX=1923404 PE=4 SV=1
MM1 pKa = 7.27 TKK3 pKa = 10.32 VNAIIDD9 pKa = 3.97 DD10 pKa = 4.09 SPVCKK15 pKa = 10.47 EE16 pKa = 3.88 SDD18 pKa = 3.92 CVGGSVWTQPTSSFLLNIRR37 pKa = 11.84 RR38 pKa = 11.84 YY39 pKa = 9.73 RR40 pKa = 11.84 FHH42 pKa = 7.48 EE43 pKa = 3.84 PHH45 pKa = 6.44 SCQACPLFKK54 pKa = 10.52 INNAVEE60 pKa = 4.59 SFFHH64 pKa = 7.63 LMFFTLAPNIVSTIMPFFQFIRR86 pKa = 11.84 KK87 pKa = 7.25 RR88 pKa = 11.84 TQGFCQHH95 pKa = 5.79 VFRR98 pKa = 11.84 KK99 pKa = 9.4 VLKK102 pKa = 10.5 AVTGLVKK109 pKa = 10.82 LGGPYY114 pKa = 8.22 ITKK117 pKa = 9.33 SWRR120 pKa = 11.84 YY121 pKa = 7.63 LCYY124 pKa = 9.61 WEE126 pKa = 5.68 ASLGYY131 pKa = 8.56 TPYY134 pKa = 10.82 RR135 pKa = 11.84 PLSDD139 pKa = 4.54 LKK141 pKa = 10.81 PDD143 pKa = 3.46 VEE145 pKa = 4.15 AWLVNPHH152 pKa = 6.42 INGEE156 pKa = 4.26 GMGTDD161 pKa = 3.46 VFHH164 pKa = 7.3 HH165 pKa = 6.63 KK166 pKa = 10.61 LYY168 pKa = 10.96 DD169 pKa = 3.54 ATCEE173 pKa = 3.98 FLRR176 pKa = 11.84 KK177 pKa = 8.15 EE178 pKa = 4.4 WKK180 pKa = 10.13 RR181 pKa = 11.84 PSNPPTLKK189 pKa = 10.04 QWLEE193 pKa = 3.5 KK194 pKa = 9.64 AAWMRR199 pKa = 11.84 GRR201 pKa = 11.84 GGTGNKK207 pKa = 8.18 STILIDD213 pKa = 3.44 NKK215 pKa = 10.3 RR216 pKa = 11.84 VKK218 pKa = 9.43 TRR220 pKa = 11.84 SNKK223 pKa = 9.74 GVDD226 pKa = 3.66 AIFLNDD232 pKa = 3.15 EE233 pKa = 3.91 ALARR237 pKa = 11.84 EE238 pKa = 4.55 MFTVIPQKK246 pKa = 10.16 MVIMQKK252 pKa = 9.88 SEE254 pKa = 3.76 PAKK257 pKa = 9.93 IRR259 pKa = 11.84 PVAKK263 pKa = 10.26 ADD265 pKa = 3.44 NQLFRR270 pKa = 11.84 KK271 pKa = 9.01 MDD273 pKa = 3.8 FLSEE277 pKa = 3.96 IVEE280 pKa = 4.34 FGLMGSKK287 pKa = 10.21 RR288 pKa = 11.84 SSLFLGPVGNEE299 pKa = 3.42 RR300 pKa = 11.84 LDD302 pKa = 3.72 LHH304 pKa = 7.96 ILDD307 pKa = 5.66 HH308 pKa = 6.3 IQHH311 pKa = 7.32 GINVPLDD318 pKa = 3.19 QGSFDD323 pKa = 4.13 NNQGKK328 pKa = 9.33 PSILVVLLAIYY339 pKa = 8.72 DD340 pKa = 3.52 VCLAQEE346 pKa = 4.35 EE347 pKa = 4.21 NHH349 pKa = 6.65 EE350 pKa = 4.2 YY351 pKa = 9.85 QKK353 pKa = 10.96 VWAAMWDD360 pKa = 4.08 SFVHH364 pKa = 6.62 PDD366 pKa = 3.04 SKK368 pKa = 11.43 VILGEE373 pKa = 5.72 DD374 pKa = 3.38 SFPWHH379 pKa = 6.61 NGVASGWRR387 pKa = 11.84 WTAFIDD393 pKa = 4.24 TILNIATYY401 pKa = 10.6 DD402 pKa = 3.54 VLTKK406 pKa = 10.69 YY407 pKa = 10.52 VSQYY411 pKa = 10.33 TGATFYY417 pKa = 11.42 DD418 pKa = 4.44 SVHH421 pKa = 6.55 QGDD424 pKa = 5.27 DD425 pKa = 3.0 IHH427 pKa = 6.74 FKK429 pKa = 10.84 CDD431 pKa = 3.22 TLGGVQTLINAYY443 pKa = 9.18 KK444 pKa = 9.77 DD445 pKa = 3.11 NGYY448 pKa = 9.55 EE449 pKa = 3.85 VHH451 pKa = 7.04 ASKK454 pKa = 9.67 TYY456 pKa = 10.74 ASTTRR461 pKa = 11.84 TEE463 pKa = 4.04 FLRR466 pKa = 11.84 RR467 pKa = 11.84 SYY469 pKa = 10.21 EE470 pKa = 3.78 KK471 pKa = 10.84 CGVLGYY477 pKa = 9.06 YY478 pKa = 10.01 CRR480 pKa = 11.84 SLQGLLYY487 pKa = 10.16 RR488 pKa = 11.84 NPITEE493 pKa = 4.0 QTILPASRR501 pKa = 11.84 IYY503 pKa = 11.12 DD504 pKa = 4.53 RR505 pKa = 11.84 ITVALLMVLRR515 pKa = 11.84 GGSPRR520 pKa = 11.84 GIAQWIRR527 pKa = 11.84 EE528 pKa = 4.39 DD529 pKa = 3.68 ASQLHH534 pKa = 5.94 VSDD537 pKa = 5.25 PEE539 pKa = 4.07 LSDD542 pKa = 5.8 FILTPSAVGGVGMSGASGPLASEE565 pKa = 4.6 FLSTATGMWTIPRR578 pKa = 11.84 SEE580 pKa = 4.02 EE581 pKa = 3.69 TSRR584 pKa = 11.84 PRR586 pKa = 11.84 CIVDD590 pKa = 3.59 LGQWNGRR597 pKa = 11.84 LQRR600 pKa = 11.84 VGVILDD606 pKa = 3.26 QTNRR610 pKa = 11.84 NNFLNDD616 pKa = 3.71 LVRR619 pKa = 11.84 TWGIRR624 pKa = 11.84 TTDD627 pKa = 3.19 VSGDD631 pKa = 3.47 FSFFWEE637 pKa = 4.67 KK638 pKa = 10.42 IKK640 pKa = 10.8 PFAPLPITGGMDD652 pKa = 4.09 LPPPSDD658 pKa = 2.32 IWADD662 pKa = 3.48 EE663 pKa = 4.17 KK664 pKa = 11.41 VPTMLRR670 pKa = 11.84 QHH672 pKa = 7.16 WKK674 pKa = 9.18 NQLIKK679 pKa = 10.94 DD680 pKa = 4.21 GTWTNHH686 pKa = 5.0 VKK688 pKa = 10.41 PEE690 pKa = 4.04 HH691 pKa = 6.57 LEE693 pKa = 3.61 QLKK696 pKa = 10.41 RR697 pKa = 11.84 LRR699 pKa = 11.84 KK700 pKa = 9.61 RR701 pKa = 11.84 ISAGLFEE708 pKa = 6.46 DD709 pKa = 4.24 YY710 pKa = 11.33 LLGTVRR716 pKa = 11.84 EE717 pKa = 4.23 PVPVVDD723 pKa = 6.2 GVALKK728 pKa = 10.7 YY729 pKa = 10.02 GQGWKK734 pKa = 9.11 QQAKK738 pKa = 7.51 QWMLRR743 pKa = 11.84 ALSSNLCDD751 pKa = 3.84 RR752 pKa = 11.84 KK753 pKa = 10.31 IFEE756 pKa = 5.65 KK757 pKa = 10.82 KK758 pKa = 9.71 MLWLEE763 pKa = 4.04 SLAIRR768 pKa = 11.84 HH769 pKa = 6.01 ISRR772 pKa = 11.84 LRR774 pKa = 11.84 LFGTLALL781 pKa = 4.43
Molecular weight: 88.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.28
IPC2_protein 8.273
IPC_protein 8.112
Toseland 8.478
ProMoST 8.682
Dawson 9.019
Bjellqvist 9.063
Wikipedia 9.092
Rodwell 9.136
Grimsley 8.726
Solomon 9.077
Lehninger 9.048
Nozaki 9.209
DTASelect 8.887
Thurlkill 8.99
EMBOSS 9.18
Sillero 9.209
Patrickios 4.647
IPC_peptide 9.063
IPC2_peptide 7.819
IPC2.peptide.svr19 7.713
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
2342
781
1561
1171.0
129.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.07 ± 1.434
1.025 ± 0.358
5.892 ± 0.216
4.056 ± 0.309
3.202 ± 0.858
6.191 ± 0.547
1.836 ± 0.477
5.124 ± 0.428
4.184 ± 0.954
10.205 ± 0.122
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.964 ± 0.19
4.611 ± 0.359
7.43 ± 1.291
4.184 ± 0.12
6.02 ± 0.07
7.857 ± 0.742
7.301 ± 0.789
6.106 ± 0.237
2.05 ± 0.501
2.69 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here