Faeces associated gemycircularvirus 18
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A160HX62|A0A160HX62_9VIRU Capsid protein OS=Faeces associated gemycircularvirus 18 OX=1843738 PE=4 SV=1
MM1 pKa = 6.76 PTFYY5 pKa = 10.64 FSARR9 pKa = 11.84 YY10 pKa = 9.46 VLLTYY15 pKa = 7.98 SQCGTLDD22 pKa = 2.99 EE23 pKa = 4.76 WDD25 pKa = 3.96 VLDD28 pKa = 5.56 HH29 pKa = 6.74 ISSLGAEE36 pKa = 4.45 CIIGRR41 pKa = 11.84 EE42 pKa = 4.05 DD43 pKa = 3.35 HH44 pKa = 7.18 ADD46 pKa = 3.64 GGTHH50 pKa = 6.1 LHH52 pKa = 5.74 VFVDD56 pKa = 5.19 FGHH59 pKa = 6.72 KK60 pKa = 9.51 KK61 pKa = 8.11 QSRR64 pKa = 11.84 RR65 pKa = 11.84 SGFFDD70 pKa = 3.79 VGGKK74 pKa = 9.26 HH75 pKa = 6.09 PNVVPSRR82 pKa = 11.84 GRR84 pKa = 11.84 AGEE87 pKa = 4.05 GWDD90 pKa = 3.6 YY91 pKa = 11.53 AVKK94 pKa = 10.58 DD95 pKa = 4.04 GNVVAGGLPRR105 pKa = 11.84 PGSGGLPSVEE115 pKa = 4.91 NIWSSIVDD123 pKa = 3.41 AQSRR127 pKa = 11.84 EE128 pKa = 4.01 EE129 pKa = 3.84 FLEE132 pKa = 4.05 LVRR135 pKa = 11.84 ALAPKK140 pKa = 10.32 EE141 pKa = 3.61 FILRR145 pKa = 11.84 HH146 pKa = 5.74 KK147 pKa = 10.33 EE148 pKa = 3.59 LLEE151 pKa = 4.09 YY152 pKa = 10.57 ADD154 pKa = 3.99 RR155 pKa = 11.84 YY156 pKa = 7.71 YY157 pKa = 11.32 AEE159 pKa = 5.19 RR160 pKa = 11.84 IEE162 pKa = 4.88 PYY164 pKa = 10.15 VGPDD168 pKa = 3.47 GIEE171 pKa = 4.18 FEE173 pKa = 4.64 LGMVPEE179 pKa = 4.18 LAGWGRR185 pKa = 11.84 EE186 pKa = 3.95 LVEE189 pKa = 4.59 ADD191 pKa = 4.15 SVTGEE196 pKa = 4.02 CPLRR200 pKa = 11.84 SGAEE204 pKa = 4.05 YY205 pKa = 10.27 ISARR209 pKa = 11.84 VV210 pKa = 3.16
Molecular weight: 23.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.906
IPC2_protein 4.965
IPC_protein 4.902
Toseland 4.825
ProMoST 5.029
Dawson 4.876
Bjellqvist 5.003
Wikipedia 4.736
Rodwell 4.787
Grimsley 4.749
Solomon 4.876
Lehninger 4.825
Nozaki 4.991
DTASelect 5.118
Thurlkill 4.825
EMBOSS 4.774
Sillero 5.067
Patrickios 3.821
IPC_peptide 4.876
IPC2_peptide 5.067
IPC2.peptide.svr19 4.978
Protein with the highest isoelectric point:
>tr|A0A160HX62|A0A160HX62_9VIRU Capsid protein OS=Faeces associated gemycircularvirus 18 OX=1843738 PE=4 SV=1
MM1 pKa = 7.75 AYY3 pKa = 10.17 ARR5 pKa = 11.84 KK6 pKa = 9.45 RR7 pKa = 11.84 RR8 pKa = 11.84 YY9 pKa = 9.7 ARR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 9.23 SGSSKK18 pKa = 10.47 GKK20 pKa = 8.41 RR21 pKa = 11.84 TTRR24 pKa = 11.84 PSARR28 pKa = 11.84 RR29 pKa = 11.84 YY30 pKa = 9.61 RR31 pKa = 11.84 STGRR35 pKa = 11.84 TRR37 pKa = 11.84 RR38 pKa = 11.84 STRR41 pKa = 11.84 TRR43 pKa = 11.84 PMSKK47 pKa = 10.29 KK48 pKa = 10.49 SILNTTSRR56 pKa = 11.84 KK57 pKa = 9.22 KK58 pKa = 10.51 RR59 pKa = 11.84 NGMLTFSNSTGTGVLASIGQNPLTIAGSAAGNNLGYY95 pKa = 10.7 VHH97 pKa = 6.95 FRR99 pKa = 11.84 PTAMDD104 pKa = 4.39 LNDD107 pKa = 4.03 NNAAPNSITTMSARR121 pKa = 11.84 TSTTCFLRR129 pKa = 11.84 GLAEE133 pKa = 4.42 NIRR136 pKa = 11.84 IEE138 pKa = 4.32 TSSGNPWFHH147 pKa = 6.87 RR148 pKa = 11.84 RR149 pKa = 11.84 ICFTSRR155 pKa = 11.84 DD156 pKa = 3.77 TLFLSVASADD166 pKa = 3.81 TPGTDD171 pKa = 2.82 RR172 pKa = 11.84 AAAASGAIEE181 pKa = 4.6 TSNGWQRR188 pKa = 11.84 LAANMALDD196 pKa = 3.86 TMTGTINNFNGLIFKK211 pKa = 7.6 GTQGRR216 pKa = 11.84 RR217 pKa = 11.84 LGMTSLLHH225 pKa = 6.92 LSILHH230 pKa = 6.77 ALTSSMTRR238 pKa = 11.84 PRR240 pKa = 11.84 FTNPGNEE247 pKa = 3.49 RR248 pKa = 11.84 GVLRR252 pKa = 11.84 EE253 pKa = 3.92 TKK255 pKa = 9.32 IWHH258 pKa = 6.52 PMNKK262 pKa = 9.98 NIVYY266 pKa = 10.5 DD267 pKa = 3.93 DD268 pKa = 4.28 DD269 pKa = 3.92 EE270 pKa = 7.1 RR271 pKa = 11.84 GDD273 pKa = 4.06 KK274 pKa = 10.84 YY275 pKa = 10.93 ATNNYY280 pKa = 9.98 SVTDD284 pKa = 3.5 KK285 pKa = 11.19 RR286 pKa = 11.84 GMGDD290 pKa = 2.99 YY291 pKa = 10.64 HH292 pKa = 8.78 IIDD295 pKa = 4.92 LFSQGSSGSTSDD307 pKa = 3.77 LLKK310 pKa = 10.32 IRR312 pKa = 11.84 YY313 pKa = 6.3 TSTMYY318 pKa = 9.55 WHH320 pKa = 7.09 EE321 pKa = 4.17 KK322 pKa = 9.5
Molecular weight: 35.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.291
IPC2_protein 9.999
IPC_protein 11.052
Toseland 11.111
ProMoST 11.257
Dawson 11.169
Bjellqvist 11.008
Wikipedia 11.506
Rodwell 11.199
Grimsley 11.213
Solomon 11.462
Lehninger 11.389
Nozaki 11.096
DTASelect 11.008
Thurlkill 11.111
EMBOSS 11.55
Sillero 11.125
Patrickios 10.906
IPC_peptide 11.462
IPC2_peptide 10.292
IPC2.peptide.svr19 8.635
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
870
210
338
290.0
32.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.356 ± 0.186
1.034 ± 0.197
5.862 ± 0.719
5.862 ± 1.772
3.793 ± 0.315
9.885 ± 0.806
2.529 ± 0.276
4.713 ± 0.156
3.908 ± 0.412
8.391 ± 0.603
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.299 ± 0.678
3.678 ± 1.579
4.483 ± 0.627
1.609 ± 0.223
8.391 ± 1.13
8.276 ± 1.042
6.207 ± 2.555
6.092 ± 1.924
1.954 ± 0.367
3.678 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here