Microcella sp. HL-107

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microcella; unclassified Microcella

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2131 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I0BJ02|A0A2I0BJ02_9MICO Cellulose biosynthesis protein BcsQ OS=Microcella sp. HL-107 OX=2035245 GN=CLT70_0499 PE=4 SV=1
MM1 pKa = 7.48RR2 pKa = 11.84RR3 pKa = 11.84IIAPMVALIAGLAVLTGVAISYY25 pKa = 8.95ALPISTQLITTAFEE39 pKa = 3.92QAGNNDD45 pKa = 3.8VEE47 pKa = 4.51WPSTSGAATEE57 pKa = 3.89FDD59 pKa = 3.54EE60 pKa = 6.15DD61 pKa = 3.7FGEE64 pKa = 4.42VEE66 pKa = 4.66VYY68 pKa = 10.52DD69 pKa = 4.14VLPDD73 pKa = 3.78ASLDD77 pKa = 3.56PAVRR81 pKa = 11.84GVAAEE86 pKa = 4.0VWDD89 pKa = 3.58QFVRR93 pKa = 11.84MVGPDD98 pKa = 3.23VAAEE102 pKa = 4.16SIIQFRR108 pKa = 11.84VGDD111 pKa = 4.22DD112 pKa = 3.59RR113 pKa = 11.84DD114 pKa = 3.98SDD116 pKa = 3.88TLAYY120 pKa = 10.23VYY122 pKa = 10.23QDD124 pKa = 3.48RR125 pKa = 11.84KK126 pKa = 8.5LQYY129 pKa = 8.19WALAVNVAYY138 pKa = 10.25ADD140 pKa = 4.27DD141 pKa = 4.24EE142 pKa = 4.41QLLLATLIHH151 pKa = 6.35EE152 pKa = 4.41WAHH155 pKa = 5.39VFSFDD160 pKa = 3.99YY161 pKa = 11.02QDD163 pKa = 3.97FDD165 pKa = 5.49RR166 pKa = 11.84KK167 pKa = 8.83TDD169 pKa = 3.47DD170 pKa = 3.77CTTIDD175 pKa = 4.61LFEE178 pKa = 4.85GCAASDD184 pKa = 3.52SYY186 pKa = 11.67LYY188 pKa = 10.69EE189 pKa = 4.75FYY191 pKa = 10.39DD192 pKa = 4.18TFWTRR197 pKa = 11.84YY198 pKa = 6.94TDD200 pKa = 4.96AVDD203 pKa = 5.59LEE205 pKa = 4.35NLDD208 pKa = 4.73PDD210 pKa = 4.44AAWEE214 pKa = 4.58FYY216 pKa = 10.56LAYY219 pKa = 10.2EE220 pKa = 4.25EE221 pKa = 5.85DD222 pKa = 4.04FVSDD226 pKa = 3.44YY227 pKa = 11.65AATNLGEE234 pKa = 4.77DD235 pKa = 3.77FAEE238 pKa = 4.21SFMTYY243 pKa = 10.15VIEE246 pKa = 5.63DD247 pKa = 3.87SFDD250 pKa = 3.7GPTVAAQKK258 pKa = 10.69LRR260 pKa = 11.84FFEE263 pKa = 4.32QYY265 pKa = 10.08PEE267 pKa = 4.01LVALRR272 pKa = 11.84EE273 pKa = 4.18HH274 pKa = 6.58IRR276 pKa = 11.84DD277 pKa = 3.52EE278 pKa = 4.32FAVEE282 pKa = 3.96LGLRR286 pKa = 3.81

Molecular weight: 32.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I0BM18|A0A2I0BM18_9MICO Uncharacterized protein (DUF427 family) OS=Microcella sp. HL-107 OX=2035245 GN=CLT70_1565 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2131

0

2131

688945

29

2877

323.3

34.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.264 ± 0.077

0.472 ± 0.013

6.154 ± 0.053

5.973 ± 0.051

2.928 ± 0.036

8.804 ± 0.05

2.045 ± 0.031

4.572 ± 0.041

1.771 ± 0.04

10.551 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.723 ± 0.02

1.866 ± 0.028

5.397 ± 0.042

2.844 ± 0.025

7.392 ± 0.069

5.738 ± 0.045

6.166 ± 0.062

9.036 ± 0.055

1.386 ± 0.023

1.917 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski