Microbacterium phage ValentiniPuff

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 112 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386KQP3|A0A386KQP3_9CAUD Membrane protein OS=Microbacterium phage ValentiniPuff OX=2315705 GN=21 PE=4 SV=1
MM1 pKa = 7.73SDD3 pKa = 3.32ATPTTEE9 pKa = 3.85GQEE12 pKa = 4.19AVSAPSRR19 pKa = 11.84PEE21 pKa = 3.9TYY23 pKa = 8.83THH25 pKa = 6.08LTVTFRR31 pKa = 11.84EE32 pKa = 4.25PLAGADD38 pKa = 3.45AVTGTVTIEE47 pKa = 3.96LEE49 pKa = 4.23EE50 pKa = 3.96IVYY53 pKa = 10.66EE54 pKa = 4.06EE55 pKa = 4.87DD56 pKa = 4.51VLDD59 pKa = 4.52EE60 pKa = 4.48YY61 pKa = 10.95PGVVHH66 pKa = 6.94PARR69 pKa = 11.84EE70 pKa = 4.13KK71 pKa = 11.16GSGGCEE77 pKa = 3.85DD78 pKa = 3.79TSDD81 pKa = 3.86VTVHH85 pKa = 6.98RR86 pKa = 11.84DD87 pKa = 3.61DD88 pKa = 3.6VDD90 pKa = 3.95EE91 pKa = 5.31IYY93 pKa = 11.31VEE95 pKa = 3.92IQKK98 pKa = 10.82ALEE101 pKa = 3.78AAGRR105 pKa = 11.84KK106 pKa = 7.19TSYY109 pKa = 9.87SWYY112 pKa = 7.2QTGDD116 pKa = 2.84IMILAFLSAAGEE128 pKa = 4.01IQTNHH133 pKa = 5.59TYY135 pKa = 8.7EE136 pKa = 4.36TDD138 pKa = 3.81GSCYY142 pKa = 10.29LVDD145 pKa = 4.11VDD147 pKa = 6.01WVDD150 pKa = 3.15EE151 pKa = 4.32WRR153 pKa = 11.84FHH155 pKa = 6.06NAA157 pKa = 2.9

Molecular weight: 17.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386KPQ7|A0A386KPQ7_9CAUD Uncharacterized protein OS=Microbacterium phage ValentiniPuff OX=2315705 GN=25 PE=4 SV=1
MM1 pKa = 7.87VSDD4 pKa = 3.7TRR6 pKa = 11.84KK7 pKa = 9.7LALSFLRR14 pKa = 11.84LPTTTQRR21 pKa = 11.84LIVTNLGLDD30 pKa = 3.26AAGDD34 pKa = 3.82DD35 pKa = 4.3RR36 pKa = 11.84LATGAWRR43 pKa = 11.84SAVLEE48 pKa = 4.07RR49 pKa = 11.84VRR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84GWQTKK58 pKa = 9.48LADD61 pKa = 5.15AIRR64 pKa = 11.84SHH66 pKa = 6.28QSYY69 pKa = 10.67ARR71 pKa = 11.84PNVPPADD78 pKa = 4.32DD79 pKa = 3.48EE80 pKa = 4.68ADD82 pKa = 4.79RR83 pKa = 11.84IRR85 pKa = 11.84QQNDD89 pKa = 2.49VSLLILTTLGVEE101 pKa = 4.47RR102 pKa = 11.84GRR104 pKa = 11.84FMVLTQRR111 pKa = 11.84VEE113 pKa = 3.69IADD116 pKa = 4.0ALWAAGYY123 pKa = 10.2RR124 pKa = 11.84KK125 pKa = 9.97VVDD128 pKa = 3.51

Molecular weight: 14.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

112

0

112

19705

37

1008

175.9

19.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.606 ± 0.405

0.624 ± 0.095

6.739 ± 0.255

6.12 ± 0.31

2.725 ± 0.132

7.922 ± 0.348

2.06 ± 0.158

5.044 ± 0.178

3.405 ± 0.213

7.556 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.482 ± 0.129

2.72 ± 0.121

5.679 ± 0.274

3.486 ± 0.139

6.455 ± 0.32

5.724 ± 0.322

7.125 ± 0.273

8.074 ± 0.222

2.116 ± 0.106

2.334 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski