Yerba mate-associated circular DNA virus 1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4ELJ5|A0A2Z4ELJ5_9VIRU Replication-associated protein OS=Yerba mate-associated circular DNA virus 1 OX=2219873 PE=4 SV=1
MM1 pKa = 7.09QLSSNSGVGTTLRR14 pKa = 11.84GEE16 pKa = 4.36AEE18 pKa = 3.9HH19 pKa = 6.93LLAEE23 pKa = 4.35EE24 pKa = 4.2RR25 pKa = 11.84AQARR29 pKa = 11.84RR30 pKa = 11.84LVRR33 pKa = 11.84TDD35 pKa = 3.67LVFLRR40 pKa = 11.84EE41 pKa = 4.22LLDD44 pKa = 3.69LAKK47 pKa = 10.44EE48 pKa = 4.41IILSGQVAWSFLGQNGAEE66 pKa = 4.36PLPHH70 pKa = 7.22AGGLTINEE78 pKa = 4.8PIRR81 pKa = 11.84LLEE84 pKa = 4.57SEE86 pKa = 5.54DD87 pKa = 3.64EE88 pKa = 4.23MSSYY92 pKa = 10.46TSSISQGDD100 pKa = 3.83LSDD103 pKa = 3.83HH104 pKa = 6.91SGGSHH109 pKa = 6.23HH110 pKa = 7.48RR111 pKa = 11.84SKK113 pKa = 11.5

Molecular weight: 12.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4ELJ7|A0A2Z4ELJ7_9VIRU Uncharacterized protein OS=Yerba mate-associated circular DNA virus 1 OX=2219873 PE=4 SV=1
MM1 pKa = 6.93VLSRR5 pKa = 11.84AEE7 pKa = 3.95RR8 pKa = 11.84SRR10 pKa = 11.84AAASRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84SNNKK21 pKa = 9.07RR22 pKa = 11.84AYY24 pKa = 8.8KK25 pKa = 10.46ALGIRR30 pKa = 11.84GRR32 pKa = 11.84DD33 pKa = 3.67EE34 pKa = 5.25LIHH37 pKa = 7.03LINQPGRR44 pKa = 11.84PVGSQRR50 pKa = 11.84RR51 pKa = 11.84FSSPLQVATFIWTVDD66 pKa = 3.32RR67 pKa = 11.84AGTPVSNTGGRR78 pKa = 11.84VDD80 pKa = 4.95LVSCYY85 pKa = 10.66GYY87 pKa = 11.24GSGDD91 pKa = 3.8HH92 pKa = 6.43EE93 pKa = 4.29RR94 pKa = 11.84HH95 pKa = 5.01SQRR98 pKa = 11.84TVTYY102 pKa = 10.53KK103 pKa = 10.02MAFNMLTCWPTDD115 pKa = 3.5DD116 pKa = 3.99TSLNLVSMCTVYY128 pKa = 10.24HH129 pKa = 5.79WLVYY133 pKa = 10.29DD134 pKa = 4.43RR135 pKa = 11.84SPDD138 pKa = 3.08ATTAEE143 pKa = 4.43FGNAQIFDD151 pKa = 3.7TGMNTMPSTWTVQRR165 pKa = 11.84DD166 pKa = 3.2KK167 pKa = 11.38SRR169 pKa = 11.84RR170 pKa = 11.84FVLKK174 pKa = 10.58KK175 pKa = 8.64RR176 pKa = 11.84WKK178 pKa = 10.03YY179 pKa = 10.43QLTHH183 pKa = 6.62NGIDD187 pKa = 3.17WRR189 pKa = 11.84VPSQTVCSSAKK200 pKa = 10.04QCVPWRR206 pKa = 11.84KK207 pKa = 8.76FVGKK211 pKa = 10.48LNVATDD217 pKa = 3.52WKK219 pKa = 10.06DD220 pKa = 3.04TATGGSVNDD229 pKa = 3.63IKK231 pKa = 11.11QGALYY236 pKa = 10.16LCSAVEE242 pKa = 4.12GVVNVVVFGRR252 pKa = 11.84MRR254 pKa = 11.84MYY256 pKa = 10.57FKK258 pKa = 10.87SIMGNN263 pKa = 3.27

Molecular weight: 29.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1146

113

338

229.2

25.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.806 ± 0.707

1.222 ± 0.424

5.148 ± 0.719

4.538 ± 0.954

3.927 ± 0.442

5.672 ± 0.865

3.229 ± 0.72

5.148 ± 1.056

3.49 ± 0.511

8.639 ± 1.425

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.094 ± 0.532

3.839 ± 0.557

5.236 ± 0.867

4.712 ± 0.714

7.243 ± 1.48

10.209 ± 1.292

7.679 ± 0.696

5.323 ± 1.317

2.443 ± 0.302

3.403 ± 0.779
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski