Schaalia turicensis ACS-279-V-Col4
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1709 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K0YXG0|K0YXG0_9ACTO Thioredoxin OS=Schaalia turicensis ACS-279-V-Col4 OX=883077 GN=HMPREF9241_00179 PE=3 SV=1
MM1 pKa = 7.77 EE2 pKa = 6.23 DD3 pKa = 3.11 GHH5 pKa = 7.46 EE6 pKa = 4.55 GEE8 pKa = 5.07 LSGEE12 pKa = 3.81 AAEE15 pKa = 5.31 AYY17 pKa = 9.55 DD18 pKa = 3.87 VLYY21 pKa = 10.89 DD22 pKa = 6.13 AITKK26 pKa = 10.26 DD27 pKa = 3.54 NQQ29 pKa = 3.12
Molecular weight: 3.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.812
IPC2_protein 3.808
IPC_protein 3.617
Toseland 3.465
ProMoST 3.821
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.579
Rodwell 3.49
Grimsley 3.401
Solomon 3.567
Lehninger 3.528
Nozaki 3.783
DTASelect 3.897
Thurlkill 3.554
EMBOSS 3.592
Sillero 3.757
Patrickios 1.837
IPC_peptide 3.567
IPC2_peptide 3.719
IPC2.peptide.svr19 3.709
Protein with the highest isoelectric point:
>tr|K0Z7Q2|K0Z7Q2_9ACTO Phosphoenolpyruvate carboxykinase [GTP] OS=Schaalia turicensis ACS-279-V-Col4 OX=883077 GN=pckG PE=3 SV=1
MM1 pKa = 5.72 TTKK4 pKa = 9.84 RR5 pKa = 11.84 TFQPNNRR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 AKK16 pKa = 8.7 THH18 pKa = 5.16 GFRR21 pKa = 11.84 LRR23 pKa = 11.84 MSTRR27 pKa = 11.84 AGRR30 pKa = 11.84 AILSSRR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 9.86 GRR41 pKa = 11.84 AKK43 pKa = 10.68 LSAA46 pKa = 3.92
Molecular weight: 5.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1709
0
1709
574013
29
1930
335.9
36.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.237 ± 0.068
0.773 ± 0.017
6.217 ± 0.041
6.16 ± 0.059
3.138 ± 0.038
8.214 ± 0.056
2.138 ± 0.026
5.412 ± 0.039
3.377 ± 0.058
9.414 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.331 ± 0.026
2.793 ± 0.038
4.797 ± 0.04
3.288 ± 0.038
6.132 ± 0.058
6.452 ± 0.061
6.256 ± 0.045
8.244 ± 0.052
1.439 ± 0.026
2.187 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here