Alphapapillomavirus 9
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A160CDW5|A0A160CDW5_9PAPI Minor capsid protein L2 OS=Alphapapillomavirus 9 OX=337041 GN=L2 PE=3 SV=1
MM1 pKa = 7.49 HH2 pKa = 7.91 GDD4 pKa = 3.35 TPTLHH9 pKa = 7.48 EE10 pKa = 4.77 YY11 pKa = 10.18 MLDD14 pKa = 3.81 LQPEE18 pKa = 4.48 TTDD21 pKa = 3.68 LYY23 pKa = 11.12 CYY25 pKa = 9.49 EE26 pKa = 4.06 QLNDD30 pKa = 3.39 SSEE33 pKa = 4.36 EE34 pKa = 3.83 EE35 pKa = 4.45 DD36 pKa = 5.2 EE37 pKa = 4.26 IDD39 pKa = 4.2 GPAGQAEE46 pKa = 4.92 PDD48 pKa = 3.3 RR49 pKa = 11.84 AHH51 pKa = 6.14 YY52 pKa = 10.59 NIVTFCCKK60 pKa = 9.95 CDD62 pKa = 3.19 STLRR66 pKa = 11.84 LCVQSTHH73 pKa = 5.81 VDD75 pKa = 2.76 IRR77 pKa = 11.84 TLEE80 pKa = 4.3 DD81 pKa = 3.76 LLIGTLGIVCPICSQKK97 pKa = 10.58 PP98 pKa = 3.28
Molecular weight: 11.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.009
IPC2_protein 4.139
IPC_protein 4.075
Toseland 3.884
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.961
Rodwell 3.91
Grimsley 3.795
Solomon 4.037
Lehninger 3.999
Nozaki 4.164
DTASelect 4.355
Thurlkill 3.923
EMBOSS 3.973
Sillero 4.19
Patrickios 0.299
IPC_peptide 4.037
IPC2_peptide 4.177
IPC2.peptide.svr19 4.089
Protein with the highest isoelectric point:
>tr|A0A160CDS1|A0A160CDS1_9PAPI Protein E7 OS=Alphapapillomavirus 9 OX=337041 GN=E7 PE=3 SV=1
YYY2 pKa = 9.99 YY3 pKa = 10.59 LHHH6 pKa = 6.91 CLAATKKK13 pKa = 10.63 YY14 pKa = 10.42 LLKKK18 pKa = 10.66 LGSTWPTTPPRR29 pKa = 11.84 PIPKKK34 pKa = 9.23 SPWAPKKK41 pKa = 8.61 KK42 pKa = 9.98 HH43 pKa = 6.07 RR44 pKa = 11.84 LSSDDD49 pKa = 2.89 DDD51 pKa = 3.36 SQTPEEE57 pKa = 4.0 PATPLSCCTEEE68 pKa = 4.29 QWTVLQSSLHHH79 pKa = 5.26 TAHHH83 pKa = 6.08 KKK85 pKa = 10.71 DD86 pKa = 3.4 LTVIVTLHHH95 pKa = 6.07
Molecular weight: 10.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.619
IPC2_protein 8.375
IPC_protein 8.434
Toseland 8.785
ProMoST 9.107
Dawson 9.107
Bjellqvist 9.194
Wikipedia 9.165
Rodwell 9.209
Grimsley 8.697
Solomon 9.37
Lehninger 9.355
Nozaki 9.399
DTASelect 9.004
Thurlkill 9.092
EMBOSS 9.297
Sillero 9.341
Patrickios 4.507
IPC_peptide 9.355
IPC2_peptide 8.17
IPC2.peptide.svr19 7.964
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2419
83
649
302.4
33.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.539 ± 0.43
3.183 ± 0.708
5.705 ± 0.44
4.423 ± 0.639
3.638 ± 0.427
5.581 ± 0.647
2.687 ± 0.64
5.663 ± 0.696
5.539 ± 0.789
8.971 ± 1.016
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.695 ± 0.306
4.506 ± 0.624
6.077 ± 1.2
4.093 ± 0.479
4.299 ± 0.477
7.606 ± 0.624
9.177 ± 1.259
5.87 ± 0.354
1.323 ± 0.344
4.423 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here