Arthrobacter phage Tatanka

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gordonvirus; unclassified Gordonvirus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5HFB5|A0A2Z5HFB5_9CAUD Uncharacterized protein OS=Arthrobacter phage Tatanka OX=2250368 GN=24 PE=4 SV=1
MM1 pKa = 6.93TTLIWDD7 pKa = 3.73VVGEE11 pKa = 4.22RR12 pKa = 11.84FYY14 pKa = 11.34EE15 pKa = 4.37AGTDD19 pKa = 3.41RR20 pKa = 11.84GVLYY24 pKa = 10.86LDD26 pKa = 4.03GVGHH30 pKa = 7.23AWHH33 pKa = 6.64GLISVEE39 pKa = 4.07QSPSGGEE46 pKa = 3.82PEE48 pKa = 4.84TYY50 pKa = 10.31YY51 pKa = 10.85QDD53 pKa = 3.36GLPYY57 pKa = 8.78VTASAAEE64 pKa = 4.08EE65 pKa = 3.95FKK67 pKa = 11.29GSIEE71 pKa = 4.41AYY73 pKa = 8.05TYY75 pKa = 10.73PNAFSACDD83 pKa = 3.79GSQEE87 pKa = 4.06IAQGLYY93 pKa = 9.94ISQQDD98 pKa = 3.54RR99 pKa = 11.84KK100 pKa = 10.21PFGLTYY106 pKa = 9.5RR107 pKa = 11.84TLIGNDD113 pKa = 3.33VQEE116 pKa = 4.81LEE118 pKa = 4.53HH119 pKa = 6.78GYY121 pKa = 10.39KK122 pKa = 10.46LHH124 pKa = 7.28ILYY127 pKa = 10.51NCMASPTTRR136 pKa = 11.84SYY138 pKa = 10.99STMGDD143 pKa = 3.16NTDD146 pKa = 3.21ASTFNWKK153 pKa = 8.17ITTRR157 pKa = 11.84PVKK160 pKa = 10.71FEE162 pKa = 4.75DD163 pKa = 3.67PSFGIKK169 pKa = 10.18YY170 pKa = 9.46GAHH173 pKa = 5.95LTLDD177 pKa = 3.52SRR179 pKa = 11.84EE180 pKa = 4.11VYY182 pKa = 8.57PWAMAAVEE190 pKa = 4.03AVLYY194 pKa = 8.58GTDD197 pKa = 3.23TTEE200 pKa = 3.97PRR202 pKa = 11.84LPTPQEE208 pKa = 3.92LLDD211 pKa = 4.24LFVDD215 pKa = 3.7NALLKK220 pKa = 9.83ITDD223 pKa = 4.09NGDD226 pKa = 3.61GSWTAEE232 pKa = 4.19GPDD235 pKa = 4.41SIISMLTDD243 pKa = 5.24DD244 pKa = 4.77MFQINWPSAVVLDD257 pKa = 4.59EE258 pKa = 4.01NTIQLSSLL266 pKa = 3.51

Molecular weight: 29.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5HEV8|A0A2Z5HEV8_9CAUD Uncharacterized protein OS=Arthrobacter phage Tatanka OX=2250368 GN=43 PE=4 SV=1
MM1 pKa = 7.29RR2 pKa = 11.84GEE4 pKa = 3.76WRR6 pKa = 11.84VIPNYY11 pKa = 9.52PLYY14 pKa = 10.44EE15 pKa = 3.86ITRR18 pKa = 11.84GGRR21 pKa = 11.84VKK23 pKa = 10.42QVLDD27 pKa = 3.48GRR29 pKa = 11.84EE30 pKa = 3.97VKK32 pKa = 9.78PANSNGTGIRR42 pKa = 11.84YY43 pKa = 9.1HH44 pKa = 7.21LEE46 pKa = 4.11SKK48 pKa = 10.12SGRR51 pKa = 11.84IVSVTRR57 pKa = 11.84SMLVSWAFPEE67 pKa = 4.36EE68 pKa = 4.33KK69 pKa = 10.62

Molecular weight: 7.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

17716

41

1529

208.4

23.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.744 ± 0.476

0.531 ± 0.091

5.983 ± 0.281

6.35 ± 0.35

3.703 ± 0.167

7.654 ± 0.464

1.914 ± 0.176

5.893 ± 0.271

6.249 ± 0.385

7.727 ± 0.415

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.845 ± 0.251

4.826 ± 0.168

4.036 ± 0.229

3.545 ± 0.154

5.075 ± 0.351

6.028 ± 0.217

6.52 ± 0.256

7.344 ± 0.201

1.564 ± 0.121

3.471 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski