Marmoricola pocheonensis
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4648 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N0GFE9|A0A3N0GFE9_9ACTN Sugar ABC transporter permease OS=Marmoricola pocheonensis OX=661485 GN=EFL26_23400 PE=4 SV=1
MM1 pKa = 7.2 YY2 pKa = 9.98 PDD4 pKa = 4.02 DD5 pKa = 4.39 LKK7 pKa = 10.95 YY8 pKa = 9.02 TAEE11 pKa = 4.35 HH12 pKa = 5.76 EE13 pKa = 4.54 WVRR16 pKa = 11.84 TPGEE20 pKa = 4.0 SAGSVRR26 pKa = 11.84 IGITDD31 pKa = 3.6 YY32 pKa = 11.6 AQDD35 pKa = 3.58 ALGDD39 pKa = 3.62 IVYY42 pKa = 10.08 VQLPEE47 pKa = 4.01 VGEE50 pKa = 4.41 SVTAGAAVGEE60 pKa = 4.3 LEE62 pKa = 4.36 STKK65 pKa = 10.63 SVSDD69 pKa = 3.57 VYY71 pKa = 11.56 SPVTGQVVARR81 pKa = 11.84 NEE83 pKa = 4.28 SLDD86 pKa = 3.62 ATPEE90 pKa = 4.02 LVNTDD95 pKa = 3.78 PYY97 pKa = 10.84 GSGWLFEE104 pKa = 4.37 VTVDD108 pKa = 4.06 DD109 pKa = 4.65 ADD111 pKa = 4.05 SGVEE115 pKa = 4.05 LLDD118 pKa = 3.72 AAAYY122 pKa = 6.74 EE123 pKa = 4.03 QLVASS128 pKa = 4.74
Molecular weight: 13.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.681
IPC_protein 3.643
Toseland 3.439
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.554
Rodwell 3.465
Grimsley 3.35
Solomon 3.605
Lehninger 3.567
Nozaki 3.757
DTASelect 3.935
Thurlkill 3.49
EMBOSS 3.567
Sillero 3.757
Patrickios 0.846
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.713
Protein with the highest isoelectric point:
>tr|A0A3N0GXP4|A0A3N0GXP4_9ACTN ABC transporter ATP-binding protein OS=Marmoricola pocheonensis OX=661485 GN=EFL26_02495 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.77 HH17 pKa = 5.81 RR18 pKa = 11.84 KK19 pKa = 8.51 LLKK22 pKa = 8.15 KK23 pKa = 9.12 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 LGKK33 pKa = 10.04
Molecular weight: 4.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4648
0
4648
1476297
31
1943
317.6
34.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.115 ± 0.056
0.745 ± 0.011
6.292 ± 0.026
5.605 ± 0.047
2.862 ± 0.02
9.089 ± 0.032
2.365 ± 0.018
3.502 ± 0.024
1.991 ± 0.023
10.308 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.875 ± 0.013
1.849 ± 0.02
5.59 ± 0.032
2.821 ± 0.022
7.717 ± 0.041
5.356 ± 0.025
6.037 ± 0.033
9.366 ± 0.037
1.53 ± 0.013
1.984 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here