Caligus rogercresseyi rhabdovirus
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L2YZV1|A0A1L2YZV1_9RHAB Glycoprotein OS=Caligus rogercresseyi rhabdovirus OX=1921414 GN=G PE=4 SV=1
MM1 pKa = 8.22 DD2 pKa = 6.52 DD3 pKa = 5.64 DD4 pKa = 5.35 IIHH7 pKa = 6.85 FKK9 pKa = 8.87 TGKK12 pKa = 9.68 VFNLLAPVEE21 pKa = 4.29 NLPTQYY27 pKa = 10.41 PATWFQQEE35 pKa = 4.12 KK36 pKa = 9.66 KK37 pKa = 10.39 KK38 pKa = 10.66 PYY40 pKa = 8.78 LTLRR44 pKa = 11.84 LPQSLIAEE52 pKa = 4.74 PARR55 pKa = 11.84 IVALLEE61 pKa = 3.84 AQMQIGRR68 pKa = 11.84 VTPDD72 pKa = 2.98 VLAFGYY78 pKa = 10.51 KK79 pKa = 9.99 FLLDD83 pKa = 3.81 RR84 pKa = 11.84 EE85 pKa = 4.47 VLTRR89 pKa = 11.84 DD90 pKa = 3.03 GPAWVSFDD98 pKa = 3.75 QQITHH103 pKa = 6.01 EE104 pKa = 4.43 NKK106 pKa = 10.13 DD107 pKa = 3.73 RR108 pKa = 11.84 LDD110 pKa = 4.93 GILTMTMQAEE120 pKa = 4.81 DD121 pKa = 3.95 GLEE124 pKa = 4.01 MKK126 pKa = 10.01 EE127 pKa = 4.22 GKK129 pKa = 7.51 PTLVDD134 pKa = 3.21 TLYY137 pKa = 11.47 VNLVVCGAIVRR148 pKa = 11.84 RR149 pKa = 11.84 DD150 pKa = 3.29 RR151 pKa = 11.84 VANVAGKK158 pKa = 10.14 DD159 pKa = 3.38 RR160 pKa = 11.84 ITQIVTRR167 pKa = 11.84 AVQSSMGIDD176 pKa = 2.88 ISGYY180 pKa = 9.22 LGSVQVTTNATTTKK194 pKa = 10.21 IMAAVDD200 pKa = 3.25 MYY202 pKa = 11.14 LYY204 pKa = 10.6 NRR206 pKa = 11.84 RR207 pKa = 11.84 DD208 pKa = 3.08 SMYY211 pKa = 10.62 GFLRR215 pKa = 11.84 LGTVCTRR222 pKa = 11.84 YY223 pKa = 9.8 RR224 pKa = 11.84 DD225 pKa = 3.85 CTVLSEE231 pKa = 4.39 FVLLEE236 pKa = 4.52 DD237 pKa = 5.58 LSQTKK242 pKa = 10.26 CKK244 pKa = 10.12 EE245 pKa = 4.12 AISWILSPIVSDD257 pKa = 3.69 EE258 pKa = 5.0 LISTMEE264 pKa = 4.68 PGQEE268 pKa = 3.91 THH270 pKa = 6.63 SVGSYY275 pKa = 8.44 MPYY278 pKa = 10.38 MMDD281 pKa = 3.35 LQLSTRR287 pKa = 11.84 SPYY290 pKa = 9.27 STSEE294 pKa = 3.64 NPKK297 pKa = 7.58 YY298 pKa = 9.29 HH299 pKa = 5.15 TWIHH303 pKa = 5.32 IVGTLLHH310 pKa = 7.0 KK311 pKa = 10.48 DD312 pKa = 3.31 RR313 pKa = 11.84 CRR315 pKa = 11.84 KK316 pKa = 9.42 AAVIEE321 pKa = 3.93 EE322 pKa = 4.12 ALIGRR327 pKa = 11.84 LFKK330 pKa = 10.85 GAALAAYY337 pKa = 8.89 RR338 pKa = 11.84 WTEE341 pKa = 3.56 SDD343 pKa = 3.19 EE344 pKa = 4.16 VVQVFGRR351 pKa = 11.84 MRR353 pKa = 11.84 DD354 pKa = 3.52 KK355 pKa = 11.12 VIEE358 pKa = 4.04 EE359 pKa = 3.96 SAAEE363 pKa = 3.83 IRR365 pKa = 11.84 RR366 pKa = 11.84 SQLEE370 pKa = 3.95 ANFLDD375 pKa = 4.38 GTDD378 pKa = 4.23 PEE380 pKa = 4.42 EE381 pKa = 4.86 DD382 pKa = 3.64 SPGAYY387 pKa = 10.01 ADD389 pKa = 3.7 WARR392 pKa = 11.84 DD393 pKa = 3.45 EE394 pKa = 4.31 YY395 pKa = 10.52 PRR397 pKa = 11.84 KK398 pKa = 10.04 LFQFIDD404 pKa = 3.46 NTLNGQFMVIRR415 pKa = 11.84 KK416 pKa = 9.66 GSMGDD421 pKa = 3.06 FVRR424 pKa = 11.84 NHH426 pKa = 5.31 WAGHH430 pKa = 6.01 RR431 pKa = 11.84 DD432 pKa = 3.3 SLVPEE437 pKa = 4.49 KK438 pKa = 11.05 FEE440 pKa = 4.14 LPPWNGSIFPNAA452 pKa = 3.87
Molecular weight: 51.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.354
IPC2_protein 5.385
IPC_protein 5.372
Toseland 5.474
ProMoST 5.575
Dawson 5.448
Bjellqvist 5.537
Wikipedia 5.372
Rodwell 5.385
Grimsley 5.474
Solomon 5.448
Lehninger 5.423
Nozaki 5.614
DTASelect 5.792
Thurlkill 5.563
EMBOSS 5.525
Sillero 5.703
Patrickios 4.215
IPC_peptide 5.461
IPC2_peptide 5.703
IPC2.peptide.svr19 5.689
Protein with the highest isoelectric point:
>tr|A0A1L2YZU8|A0A1L2YZU8_9RHAB Uncharacterized protein OS=Caligus rogercresseyi rhabdovirus OX=1921414 PE=4 SV=1
MM1 pKa = 7.39 KK2 pKa = 10.35 KK3 pKa = 9.71 IFRR6 pKa = 11.84 SKK8 pKa = 10.71 RR9 pKa = 11.84 LGSTPVASAPTVPGPWRR26 pKa = 11.84 ISPQEE31 pKa = 4.1 DD32 pKa = 3.27 LSKK35 pKa = 10.75 RR36 pKa = 11.84 GASAGPSKK44 pKa = 9.99 TKK46 pKa = 10.66 GQGSPQCNCRR56 pKa = 11.84 EE57 pKa = 3.77 VKK59 pKa = 10.55 LRR61 pKa = 11.84 IKK63 pKa = 10.26 GHH65 pKa = 5.46 LSIRR69 pKa = 11.84 LGSSPRR75 pKa = 11.84 TRR77 pKa = 11.84 EE78 pKa = 3.61 QAQYY82 pKa = 11.48 ACTNFLDD89 pKa = 3.85 QYY91 pKa = 10.88 GEE93 pKa = 4.36 SIEE96 pKa = 4.67 KK97 pKa = 10.6 SGLYY101 pKa = 5.68 WTAVIILARR110 pKa = 11.84 KK111 pKa = 7.76 LQSVHH116 pKa = 6.73 PGGGNSDD123 pKa = 3.67 YY124 pKa = 10.64 TASLDD129 pKa = 3.79 EE130 pKa = 5.22 NISILTTIASDD141 pKa = 4.19 LLNDD145 pKa = 3.52 IPKK148 pKa = 10.66 LEE150 pKa = 4.12 MSYY153 pKa = 8.98 GTQEE157 pKa = 4.51 GISGRR162 pKa = 11.84 FGIISIQLTPTRR174 pKa = 11.84 SPALPLRR181 pKa = 11.84 AVLAKK186 pKa = 10.26 IKK188 pKa = 10.73 LPAEE192 pKa = 4.28 KK193 pKa = 9.37 FIKK196 pKa = 9.54 MAEE199 pKa = 4.38 TFGVTYY205 pKa = 10.26 EE206 pKa = 4.67 LIQGKK211 pKa = 9.17 IVLHH215 pKa = 5.58 TTTQSRR221 pKa = 11.84 SHH223 pKa = 6.29 LRR225 pKa = 11.84 EE226 pKa = 3.76 VVLL229 pKa = 4.9
Molecular weight: 25.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.105
IPC2_protein 9.224
IPC_protein 9.194
Toseland 10.043
ProMoST 9.663
Dawson 10.204
Bjellqvist 9.867
Wikipedia 10.35
Rodwell 10.687
Grimsley 10.262
Solomon 10.233
Lehninger 10.218
Nozaki 10.058
DTASelect 9.838
Thurlkill 10.072
EMBOSS 10.438
Sillero 10.131
Patrickios 10.35
IPC_peptide 10.248
IPC2_peptide 8.492
IPC2.peptide.svr19 8.174
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3737
136
2130
622.8
70.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.272 ± 0.465
1.525 ± 0.512
5.727 ± 0.313
6.342 ± 0.345
3.746 ± 0.395
6.396 ± 0.31
2.275 ± 0.272
5.941 ± 0.434
5.539 ± 0.67
10.891 ± 0.758
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.034 ± 0.323
3.72 ± 0.286
4.977 ± 0.376
3.372 ± 0.361
6.904 ± 0.498
8.188 ± 0.46
6.529 ± 0.595
5.593 ± 0.316
1.82 ± 0.237
3.211 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here