Cellulomonas sp. SLBN-39

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Cellulomonas; unclassified Cellulomonas

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3709 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A542UX29|A0A542UX29_9CELL N-acetylneuraminate synthase OS=Cellulomonas sp. SLBN-39 OX=2768446 GN=FBY24_2753 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84MLAFAAAAAVLLTAACGGGDD24 pKa = 3.43EE25 pKa = 4.61AATGGEE31 pKa = 4.57TTGSTASLVLGQTDD45 pKa = 3.45IPASFDD51 pKa = 3.34VLGYY55 pKa = 11.16ASGTKK60 pKa = 7.66TQYY63 pKa = 8.72FTAVYY68 pKa = 7.78DD69 pKa = 3.62TLLRR73 pKa = 11.84QDD75 pKa = 3.17GSGAIVPGLATDD87 pKa = 3.82WAYY90 pKa = 11.21DD91 pKa = 3.53DD92 pKa = 3.87TRR94 pKa = 11.84TVLTLTLRR102 pKa = 11.84EE103 pKa = 4.29GVTFTDD109 pKa = 5.54GVALDD114 pKa = 4.14ADD116 pKa = 3.52AVVANIEE123 pKa = 4.18HH124 pKa = 6.36FRR126 pKa = 11.84GSSTPDD132 pKa = 3.32LSNAAYY138 pKa = 9.39IDD140 pKa = 3.92TVSAVDD146 pKa = 3.36ATTVEE151 pKa = 4.21ITLTEE156 pKa = 4.83PDD158 pKa = 3.86PMMLTWLTQPLGYY171 pKa = 9.47MSSPEE176 pKa = 3.7SWEE179 pKa = 4.17NEE181 pKa = 3.84DD182 pKa = 4.01VATNPVGSGPYY193 pKa = 10.27VLDD196 pKa = 3.51TEE198 pKa = 4.46ATVVGSTYY206 pKa = 10.93VFTKK210 pKa = 10.82NPDD213 pKa = 3.09YY214 pKa = 10.8WDD216 pKa = 5.22DD217 pKa = 3.71SLQLFDD223 pKa = 4.51TLTMNYY229 pKa = 9.97YY230 pKa = 10.83ADD232 pKa = 3.99QSALLNALQGGQVDD246 pKa = 4.11AAPFNQFSALEE257 pKa = 3.99QVEE260 pKa = 4.22AAGYY264 pKa = 7.96EE265 pKa = 4.66SNVSRR270 pKa = 11.84LDD272 pKa = 3.32WEE274 pKa = 4.63GLMLLDD280 pKa = 5.23RR281 pKa = 11.84DD282 pKa = 4.19GALDD286 pKa = 3.69PALADD291 pKa = 3.24VRR293 pKa = 11.84VRR295 pKa = 11.84QAINHH300 pKa = 7.16AIAKK304 pKa = 10.06DD305 pKa = 3.99SILSAILLDD314 pKa = 4.17HH315 pKa = 6.48GTATSQIFGPSTDD328 pKa = 4.53GYY330 pKa = 11.33DD331 pKa = 3.43EE332 pKa = 5.13SLDD335 pKa = 3.51GYY337 pKa = 8.47YY338 pKa = 10.2TYY340 pKa = 11.35DD341 pKa = 3.61PDD343 pKa = 4.12KK344 pKa = 11.4AKK346 pKa = 10.99ALLAEE351 pKa = 4.66AGYY354 pKa = 11.36ADD356 pKa = 4.14GLEE359 pKa = 4.14LTMPSTSVIDD369 pKa = 3.76QALLTSIQQQLADD382 pKa = 3.48VGITVSYY389 pKa = 10.34TDD391 pKa = 3.15VGTNFISDD399 pKa = 3.86LMGAKK404 pKa = 9.97YY405 pKa = 10.3GSSWMRR411 pKa = 11.84LASSSDD417 pKa = 2.77WQTATFALTPDD428 pKa = 3.25ALFNSFRR435 pKa = 11.84TQRR438 pKa = 11.84PEE440 pKa = 3.25VDD442 pKa = 3.46ALLEE446 pKa = 4.2TVQKK450 pKa = 9.77GTEE453 pKa = 3.98EE454 pKa = 3.91EE455 pKa = 3.88AAAAAQEE462 pKa = 4.06LNRR465 pKa = 11.84YY466 pKa = 7.53VVEE469 pKa = 4.35EE470 pKa = 3.78AWFAPFYY477 pKa = 10.73FVDD480 pKa = 3.37NVYY483 pKa = 10.43VSKK486 pKa = 9.05PTLDD490 pKa = 3.43VTPAADD496 pKa = 3.28MVVPYY501 pKa = 10.42LYY503 pKa = 10.56LIQPAAA509 pKa = 3.62

Molecular weight: 54.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A542UW40|A0A542UW40_9CELL NADH-quinone oxidoreductase subunit C OS=Cellulomonas sp. SLBN-39 OX=2768446 GN=nuoC PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3709

0

3709

1256622

30

2247

338.8

35.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.197 ± 0.064

0.542 ± 0.01

6.676 ± 0.035

4.884 ± 0.043

2.262 ± 0.024

9.517 ± 0.041

2.187 ± 0.02

2.224 ± 0.028

1.102 ± 0.025

10.288 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.451 ± 0.016

1.282 ± 0.02

6.289 ± 0.036

2.679 ± 0.021

8.007 ± 0.05

4.475 ± 0.033

6.727 ± 0.052

10.936 ± 0.056

1.577 ± 0.016

1.697 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski