Chryseobacterium sp.
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3175 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A519LI26|A0A519LI26_9FLAO Restriction endonuclease (Fragment) OS=Chryseobacterium sp. OX=1871047 GN=EOO19_06625 PE=4 SV=1
MM1 pKa = 7.38 NEE3 pKa = 3.19 SWLQKK8 pKa = 9.2 WEE10 pKa = 4.01 EE11 pKa = 4.07 VKK13 pKa = 10.9 DD14 pKa = 3.9 VLVCPTDD21 pKa = 3.36 LEE23 pKa = 4.98 TYY25 pKa = 7.49 FTSNEE30 pKa = 3.57 ILGQKK35 pKa = 8.89 MEE37 pKa = 4.14 TMEE40 pKa = 4.62 IGNVSLPSGKK50 pKa = 9.48 IVVRR54 pKa = 11.84 DD55 pKa = 3.49 PLVYY59 pKa = 10.59 LSSDD63 pKa = 3.31 EE64 pKa = 4.1 KK65 pKa = 10.95 PYY67 pKa = 10.59 FIQAPKK73 pKa = 11.1 GNFLVTIAVVKK84 pKa = 10.92 SEE86 pKa = 4.12 DD87 pKa = 3.02 WGDD90 pKa = 3.2 RR91 pKa = 11.84 YY92 pKa = 11.02 AVVKK96 pKa = 10.33 VQFTQKK102 pKa = 10.38 KK103 pKa = 7.31 PVVYY107 pKa = 10.09 RR108 pKa = 11.84 EE109 pKa = 3.98 ALIGIEE115 pKa = 4.13 DD116 pKa = 4.29 LEE118 pKa = 5.15 DD119 pKa = 3.35 VTEE122 pKa = 4.74 DD123 pKa = 3.63 DD124 pKa = 4.57 FFGFSVDD131 pKa = 4.21 AGLGCITDD139 pKa = 4.17 AEE141 pKa = 4.47 VLPSVDD147 pKa = 3.26 QFVAATDD154 pKa = 3.41 VDD156 pKa = 4.11 NVYY159 pKa = 11.28 DD160 pKa = 4.54 DD161 pKa = 4.1 YY162 pKa = 11.27 FAEE165 pKa = 5.16 IFAQSYY171 pKa = 10.11 KK172 pKa = 10.74 DD173 pKa = 3.41 HH174 pKa = 7.47 PNNQRR179 pKa = 11.84 DD180 pKa = 3.45 AGDD183 pKa = 4.08 WINWTVPNTDD193 pKa = 3.05 YY194 pKa = 10.66 QIPMFASGFGDD205 pKa = 3.74 GSYY208 pKa = 10.54 PVYY211 pKa = 10.07 FAYY214 pKa = 10.21 DD215 pKa = 3.32 AHH217 pKa = 8.6 GEE219 pKa = 3.97 ICGLYY224 pKa = 9.45 IQFIDD229 pKa = 3.8 VEE231 pKa = 4.31 LALTEE236 pKa = 5.24 DD237 pKa = 4.46 GDD239 pKa = 4.84 DD240 pKa = 4.04 EE241 pKa = 5.17 DD242 pKa = 5.56 VEE244 pKa = 4.43
Molecular weight: 27.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.808
IPC_protein 3.808
Toseland 3.592
ProMoST 3.948
Dawson 3.795
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.139
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.935
Patrickios 1.875
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A519KY54|A0A519KY54_9FLAO Non-ribosomal peptide synthetase (Fragment) OS=Chryseobacterium sp. OX=1871047 GN=EOO19_14150 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPSEE10 pKa = 3.59 RR11 pKa = 11.84 KK12 pKa = 9.33 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 10.03 HH17 pKa = 3.97 GFRR20 pKa = 11.84 EE21 pKa = 4.33 RR22 pKa = 11.84 MSTPNGRR29 pKa = 11.84 RR30 pKa = 11.84 VLAARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.98 GRR40 pKa = 11.84 KK41 pKa = 8.8 SLTVSAQRR49 pKa = 11.84 AKK51 pKa = 10.68 RR52 pKa = 3.52
Molecular weight: 6.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.365
IPC2_protein 10.774
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.223
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.959
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.11
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3175
0
3175
888173
23
1977
279.7
31.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.965 ± 0.042
0.74 ± 0.013
5.608 ± 0.031
7.134 ± 0.053
5.527 ± 0.033
5.916 ± 0.042
1.744 ± 0.021
7.992 ± 0.039
8.727 ± 0.045
9.108 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.271 ± 0.02
6.339 ± 0.046
3.294 ± 0.027
3.598 ± 0.026
3.268 ± 0.028
6.597 ± 0.036
5.299 ± 0.036
5.842 ± 0.03
1.052 ± 0.015
3.981 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here