Ulvibacter sp. KK4
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2972 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5J4G032|A0A5J4G032_9FLAO 6-carboxy-5 6 7 8-tetrahydropterin synthase OS=Ulvibacter sp. KK4 OX=2494560 GN=ygcM PE=3 SV=1
MM1 pKa = 7.66 KK2 pKa = 9.59 NTFILLLIAISLWSCEE18 pKa = 3.74 EE19 pKa = 4.15 VIEE22 pKa = 4.86 VEE24 pKa = 4.81 LDD26 pKa = 3.19 TTAPRR31 pKa = 11.84 LVIDD35 pKa = 3.92 ASINILLDD43 pKa = 4.34 DD44 pKa = 4.74 GSVTPKK50 pKa = 10.38 IKK52 pKa = 10.63 LSEE55 pKa = 3.96 TAPFFGNEE63 pKa = 4.03 VPPVTNAIVSITSDD77 pKa = 3.26 NNEE80 pKa = 3.45 VFMFEE85 pKa = 4.06 HH86 pKa = 6.26 QGDD89 pKa = 4.21 GEE91 pKa = 4.52 YY92 pKa = 7.84 TTAIVPQLGVNYY104 pKa = 8.29 TLKK107 pKa = 10.39 IIHH110 pKa = 6.66 NDD112 pKa = 2.94 EE113 pKa = 4.52 TYY115 pKa = 10.19 TATTSLQTVVPLEE128 pKa = 4.07 EE129 pKa = 3.93 VTQNNEE135 pKa = 3.61 GGFTGDD141 pKa = 3.46 MIEE144 pKa = 4.25 VEE146 pKa = 4.58 ISFTDD151 pKa = 3.81 PSNEE155 pKa = 3.41 NNYY158 pKa = 10.11 YY159 pKa = 10.34 FFEE162 pKa = 4.55 GLSDD166 pKa = 3.86 RR167 pKa = 11.84 GDD169 pKa = 3.68 LRR171 pKa = 11.84 DD172 pKa = 3.65 SFSDD176 pKa = 3.33 EE177 pKa = 4.1 FFSGNTFQAIYY188 pKa = 9.62 FADD191 pKa = 5.21 DD192 pKa = 3.58 IEE194 pKa = 6.14 PDD196 pKa = 3.68 DD197 pKa = 4.87 VISFSLYY204 pKa = 10.33 GVDD207 pKa = 3.15 EE208 pKa = 3.97 QFYY211 pKa = 10.44 NFMFALLQQSSDD223 pKa = 3.01 GGGPFEE229 pKa = 4.55 TQPATIRR236 pKa = 11.84 GNIINEE242 pKa = 4.43 TNTDD246 pKa = 3.91 NYY248 pKa = 9.38 PLGYY252 pKa = 10.09 FRR254 pKa = 11.84 ISEE257 pKa = 4.16 LSKK260 pKa = 10.38 IVYY263 pKa = 8.58 TVEE266 pKa = 3.62
Molecular weight: 29.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.719
IPC_protein 3.706
Toseland 3.503
ProMoST 3.834
Dawson 3.681
Bjellqvist 3.872
Wikipedia 3.592
Rodwell 3.541
Grimsley 3.414
Solomon 3.668
Lehninger 3.63
Nozaki 3.795
DTASelect 3.973
Thurlkill 3.541
EMBOSS 3.605
Sillero 3.821
Patrickios 1.1
IPC_peptide 3.668
IPC2_peptide 3.808
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|A0A5J4FUW5|A0A5J4FUW5_9FLAO Uncharacterized protein OS=Ulvibacter sp. KK4 OX=2494560 GN=ULMS_03420 PE=4 SV=1
MM1 pKa = 7.54 SVRR4 pKa = 11.84 KK5 pKa = 9.68 LKK7 pKa = 10.5 PITPGQRR14 pKa = 11.84 FRR16 pKa = 11.84 VVNGFDD22 pKa = 5.49 AITTDD27 pKa = 3.56 KK28 pKa = 11.07 PEE30 pKa = 4.39 KK31 pKa = 10.58 SLLAPNKK38 pKa = 10.02 RR39 pKa = 11.84 SGGRR43 pKa = 11.84 NSQGKK48 pKa = 6.48 MTMRR52 pKa = 11.84 YY53 pKa = 9.3 IGGGHH58 pKa = 5.85 KK59 pKa = 9.86 KK60 pKa = 9.7 KK61 pKa = 10.84 YY62 pKa = 10.14 RR63 pKa = 11.84 IIDD66 pKa = 3.85 FKK68 pKa = 10.78 RR69 pKa = 11.84 DD70 pKa = 3.38 KK71 pKa = 11.03 AGVPATVATIEE82 pKa = 4.01 YY83 pKa = 9.78 DD84 pKa = 3.51 PNRR87 pKa = 11.84 TAFIALLNYY96 pKa = 9.66 QDD98 pKa = 3.47 GEE100 pKa = 4.05 KK101 pKa = 10.22 RR102 pKa = 11.84 YY103 pKa = 10.72 VIAQNGLQVGQNIVSGEE120 pKa = 3.93 QTTPEE125 pKa = 3.97 IGNAMVLANVPLGTIISCIEE145 pKa = 3.75 LRR147 pKa = 11.84 PGQGAVLARR156 pKa = 11.84 SAGAFAQLMARR167 pKa = 11.84 DD168 pKa = 3.96 GKK170 pKa = 10.52 YY171 pKa = 9.12 ATVKK175 pKa = 10.42 LPSGEE180 pKa = 4.05 TRR182 pKa = 11.84 LILVTCMATIGAVSNSDD199 pKa = 3.2 HH200 pKa = 6.21 QLLVSGKK207 pKa = 9.49 AGRR210 pKa = 11.84 SRR212 pKa = 11.84 WLGRR216 pKa = 11.84 RR217 pKa = 11.84 PRR219 pKa = 11.84 TRR221 pKa = 11.84 PVVMNPVDD229 pKa = 3.47 HH230 pKa = 7.05 PMGGGEE236 pKa = 4.28 GKK238 pKa = 10.51 SSGGHH243 pKa = 4.59 PRR245 pKa = 11.84 SRR247 pKa = 11.84 NGIPAKK253 pKa = 10.44 GFRR256 pKa = 11.84 TRR258 pKa = 11.84 SKK260 pKa = 9.1 TKK262 pKa = 10.18 QSNKK266 pKa = 10.2 YY267 pKa = 9.54 IIEE270 pKa = 4.05 RR271 pKa = 11.84 RR272 pKa = 11.84 KK273 pKa = 10.1 KK274 pKa = 9.82
Molecular weight: 29.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.313
IPC2_protein 9.926
IPC_protein 10.789
Toseland 11.052
ProMoST 10.847
Dawson 11.111
Bjellqvist 10.847
Wikipedia 11.345
Rodwell 11.359
Grimsley 11.14
Solomon 11.286
Lehninger 11.257
Nozaki 11.023
DTASelect 10.833
Thurlkill 11.038
EMBOSS 11.462
Sillero 11.052
Patrickios 11.082
IPC_peptide 11.301
IPC2_peptide 9.78
IPC2.peptide.svr19 8.494
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2972
0
2972
989612
42
3042
333.0
37.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.585 ± 0.042
0.725 ± 0.02
5.714 ± 0.037
6.645 ± 0.047
5.185 ± 0.036
6.561 ± 0.068
1.644 ± 0.022
8.103 ± 0.043
7.572 ± 0.081
8.946 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.253 ± 0.024
6.43 ± 0.057
3.336 ± 0.028
3.307 ± 0.026
3.349 ± 0.034
6.406 ± 0.038
6.198 ± 0.062
6.248 ± 0.036
0.954 ± 0.015
3.837 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here