Bifidobacterium choerinum
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1672 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A087AE55|A0A087AE55_9BIFI Putative serine/threonine-protein kinase PknK OS=Bifidobacterium choerinum OX=35760 GN=BCHO_0729 PE=4 SV=1
MM1 pKa = 7.33 VSYY4 pKa = 10.82 DD5 pKa = 3.58 DD6 pKa = 4.52 AVAIIQDD13 pKa = 3.81 PQADD17 pKa = 4.1 PVTLAKK23 pKa = 10.04 VAYY26 pKa = 7.25 EE27 pKa = 3.96 NPEE30 pKa = 3.71 FGANVAAHH38 pKa = 5.94 PRR40 pKa = 11.84 AYY42 pKa = 9.64 PGLLRR47 pKa = 11.84 WIAQFGDD54 pKa = 3.02 EE55 pKa = 4.17 RR56 pKa = 11.84 ARR58 pKa = 11.84 ATVAQLGYY66 pKa = 10.01 HH67 pKa = 5.39 SQLGAVEE74 pKa = 4.38 DD75 pKa = 4.23 RR76 pKa = 11.84 QVDD79 pKa = 3.45 AAAIDD84 pKa = 3.66 EE85 pKa = 4.5 AAAIAAAIDD94 pKa = 3.54 AAQANAASRR103 pKa = 11.84 PTAAASPAVDD113 pKa = 3.48 NVAGGLVMNAATAGDD128 pKa = 4.06 EE129 pKa = 4.18 AQPIASQTGTVEE141 pKa = 4.17 YY142 pKa = 10.52 ASVEE146 pKa = 3.98 AQPVSFEE153 pKa = 4.21 PAQQTGVDD161 pKa = 3.64 MLSEE165 pKa = 4.02 QFNNIGMDD173 pKa = 3.75 ASQTNMDD180 pKa = 4.22 ASQSAAASPSVAAPDD195 pKa = 3.93 MAQQPMMDD203 pKa = 3.39 QAQPADD209 pKa = 3.92 AYY211 pKa = 10.9 AQIQATNPYY220 pKa = 9.94 GFTAEE225 pKa = 4.23 VAMTTPDD232 pKa = 3.55 TTVMQQIAQYY242 pKa = 10.91 APEE245 pKa = 4.46 LHH247 pKa = 6.85 PALAQNPYY255 pKa = 9.92 IYY257 pKa = 10.08 PEE259 pKa = 4.23 LLSWLGMLGEE269 pKa = 4.48 PATNASIAQRR279 pKa = 11.84 QQQQ282 pKa = 3.02
Molecular weight: 29.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.821
IPC_protein 3.808
Toseland 3.592
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.126
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.923
Patrickios 1.062
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.824
Protein with the highest isoelectric point:
>tr|A0A087AGV6|A0A087AGV6_9BIFI Tellurite resistance protein TerB OS=Bifidobacterium choerinum OX=35760 GN=BCHO_0086 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 4.79 MKK15 pKa = 9.36 HH16 pKa = 5.81 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 SGRR28 pKa = 11.84 ALINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.1 GRR39 pKa = 11.84 KK40 pKa = 6.41 TLSAA44 pKa = 4.15
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1672
0
1672
597285
29
3109
357.2
39.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.639 ± 0.086
0.979 ± 0.019
7.262 ± 0.057
5.714 ± 0.057
3.326 ± 0.037
7.97 ± 0.044
2.3 ± 0.026
5.089 ± 0.043
3.484 ± 0.053
8.583 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.715 ± 0.024
2.976 ± 0.042
4.743 ± 0.04
3.309 ± 0.04
6.64 ± 0.065
5.351 ± 0.047
6.0 ± 0.052
7.885 ± 0.052
1.354 ± 0.025
2.683 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here