Trichodysplasia spinulosa-associated polyomavirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Alphapolyomavirus; Human polyomavirus 8

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E2ESL6|E2ESL6_9POLY Minor capsid protein VP2 OS=Trichodysplasia spinulosa-associated polyomavirus OX=862909 GN=VP3 PE=3 SV=1
MM1 pKa = 7.47GGVISLFFDD10 pKa = 4.06IIEE13 pKa = 4.36ISTEE17 pKa = 3.59LSAATGFAVDD27 pKa = 3.91AVLAGEE33 pKa = 4.1AAAAVEE39 pKa = 4.28VEE41 pKa = 4.43VMGLMTVEE49 pKa = 4.33GLSASEE55 pKa = 4.01ALGTLGLTMEE65 pKa = 5.06NFSLMHH71 pKa = 7.14ALPGMLSEE79 pKa = 4.54AVGIGTLFQTISGASGLVAAGIRR102 pKa = 11.84YY103 pKa = 9.05GYY105 pKa = 10.05ARR107 pKa = 11.84EE108 pKa = 3.77VSIVNRR114 pKa = 11.84NISQMALQVWRR125 pKa = 11.84PWDD128 pKa = 3.54YY129 pKa = 11.83YY130 pKa = 11.28DD131 pKa = 3.81ILFPGVQTFAHH142 pKa = 5.91YY143 pKa = 11.18LNVLDD148 pKa = 4.88HH149 pKa = 6.52WASSLIHH156 pKa = 5.26TVSRR160 pKa = 11.84YY161 pKa = 7.19VWDD164 pKa = 5.56AILHH168 pKa = 5.7EE169 pKa = 4.76GRR171 pKa = 11.84HH172 pKa = 5.16QIGHH176 pKa = 7.33ASRR179 pKa = 11.84EE180 pKa = 3.95LMIRR184 pKa = 11.84GTNHH188 pKa = 5.86FQDD191 pKa = 3.52LMARR195 pKa = 11.84LIEE198 pKa = 3.94NSRR201 pKa = 11.84WVLTTGPSNIYY212 pKa = 10.14SHH214 pKa = 7.0LEE216 pKa = 3.56SYY218 pKa = 10.95YY219 pKa = 10.43RR220 pKa = 11.84DD221 pKa = 3.62LPGISPPQARR231 pKa = 11.84DD232 pKa = 3.15LYY234 pKa = 11.02RR235 pKa = 11.84RR236 pKa = 11.84LQEE239 pKa = 4.75KK240 pKa = 10.39IPDD243 pKa = 3.97RR244 pKa = 11.84YY245 pKa = 10.12QLEE248 pKa = 4.17AATDD252 pKa = 3.61EE253 pKa = 4.27SAEE256 pKa = 4.17VIEE259 pKa = 5.11TYY261 pKa = 10.25SAPGGAHH268 pKa = 6.07QRR270 pKa = 11.84VCPDD274 pKa = 2.28WMLPLVLGLYY284 pKa = 10.89GDD286 pKa = 3.82ITPTFGYY293 pKa = 9.37YY294 pKa = 10.07LRR296 pKa = 11.84EE297 pKa = 4.24EE298 pKa = 4.19EE299 pKa = 5.83RR300 pKa = 11.84EE301 pKa = 4.08DD302 pKa = 4.01GPQKK306 pKa = 9.98KK307 pKa = 9.09RR308 pKa = 11.84RR309 pKa = 11.84RR310 pKa = 11.84MRR312 pKa = 3.59

Molecular weight: 34.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E2ESL9|E2ESL9_9POLY Isoform of E2ESL8 Small T antigen OS=Trichodysplasia spinulosa-associated polyomavirus OX=862909 GN=T PE=4 SV=1
MM1 pKa = 8.02DD2 pKa = 4.71KK3 pKa = 10.5FLSRR7 pKa = 11.84EE8 pKa = 3.83EE9 pKa = 4.2SLEE12 pKa = 4.7LMDD15 pKa = 5.59LLQIPRR21 pKa = 11.84HH22 pKa = 5.91CYY24 pKa = 10.76GNFALMKK31 pKa = 9.53INHH34 pKa = 6.61KK35 pKa = 10.58KK36 pKa = 9.86MSLKK40 pKa = 9.98YY41 pKa = 10.25HH42 pKa = 6.86PDD44 pKa = 3.2KK45 pKa = 11.6GGDD48 pKa = 3.62PEE50 pKa = 4.85KK51 pKa = 10.3MSRR54 pKa = 11.84LNQLWQKK61 pKa = 9.89LQEE64 pKa = 4.63GIYY67 pKa = 10.02NARR70 pKa = 11.84QEE72 pKa = 4.44FPTSFSSQVGSWYY85 pKa = 9.79WEE87 pKa = 3.89ANLISLKK94 pKa = 10.63EE95 pKa = 3.88YY96 pKa = 10.45FGKK99 pKa = 10.4KK100 pKa = 9.98KK101 pKa = 10.51YY102 pKa = 10.12DD103 pKa = 3.56EE104 pKa = 4.47NVIKK108 pKa = 10.21HH109 pKa = 5.71WPQCAEE115 pKa = 4.2KK116 pKa = 10.57ALKK119 pKa = 9.61EE120 pKa = 4.47CKK122 pKa = 10.23CLTCKK127 pKa = 10.23IGLQHH132 pKa = 6.61HH133 pKa = 6.95VYY135 pKa = 10.61KK136 pKa = 10.85QMHH139 pKa = 5.09QKK141 pKa = 10.22KK142 pKa = 8.66CVVWGEE148 pKa = 4.11CFCYY152 pKa = 10.02KK153 pKa = 10.3CYY155 pKa = 10.41CAWFGEE161 pKa = 4.26DD162 pKa = 5.38LYY164 pKa = 11.69CLDD167 pKa = 5.53SLWAWSCIVGEE178 pKa = 4.27VDD180 pKa = 3.83FHH182 pKa = 7.3LVNLYY187 pKa = 10.79LRR189 pKa = 11.84VNQGFNWVFPFSMMFQPRR207 pKa = 11.84MEE209 pKa = 4.89EE210 pKa = 3.67IYY212 pKa = 10.8LPMGTPPGPAGGKK225 pKa = 10.14ASIKK229 pKa = 10.89NGTTCLTPCRR239 pKa = 11.84TQISSAMNPPFPLMNLDD256 pKa = 3.51LQAPLRR262 pKa = 11.84DD263 pKa = 3.61PLLNLARR270 pKa = 11.84RR271 pKa = 11.84IQEE274 pKa = 4.11EE275 pKa = 4.47EE276 pKa = 4.13EE277 pKa = 4.55LPHH280 pKa = 6.72QRR282 pKa = 11.84TPPAAPRR289 pKa = 11.84APSLPPPQSQKK300 pKa = 10.63NLSMTLSLMIFLICCGLFFLMLSIVIKK327 pKa = 10.42LYY329 pKa = 10.94HH330 pKa = 6.53LFF332 pKa = 4.02

Molecular weight: 38.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

2

6

2108

194

697

351.3

40.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.981 ± 0.833

2.846 ± 0.656

4.981 ± 0.408

6.784 ± 0.514

4.317 ± 0.73

6.12 ± 1.048

2.799 ± 0.355

4.412 ± 0.441

6.452 ± 1.418

10.389 ± 0.421

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.226 ± 0.304

4.507 ± 0.623

6.025 ± 0.669

4.269 ± 0.273

4.744 ± 0.801

6.357 ± 0.412

4.934 ± 0.764

5.55 ± 0.576

1.945 ± 0.426

4.364 ± 0.531
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski