Sporobacter termitidis DSM 10068

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Sporobacter; Sporobacter termitidis

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3771 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5WIX7|A0A1M5WIX7_9FIRM Cysteine desulfurase OS=Sporobacter termitidis DSM 10068 OX=1123282 GN=SAMN02745823_01303 PE=3 SV=1
MM1 pKa = 7.2TPEE4 pKa = 4.19ASAPSPTPDD13 pKa = 4.19AAPLPPGAFAVSSGNIHH30 pKa = 7.75DD31 pKa = 4.76YY32 pKa = 11.26LPDD35 pKa = 5.08LIVGKK40 pKa = 9.18PVSAYY45 pKa = 10.71ALLPCLDD52 pKa = 3.79SFSRR56 pKa = 11.84GTWSEE61 pKa = 4.23LEE63 pKa = 4.12TAYY66 pKa = 10.47GGPDD70 pKa = 3.27GTDD73 pKa = 2.14WWSPLLTALNDD84 pKa = 3.53SAAGDD89 pKa = 3.94DD90 pKa = 3.76QEE92 pKa = 4.39MRR94 pKa = 11.84DD95 pKa = 4.04YY96 pKa = 11.31YY97 pKa = 10.96LGRR100 pKa = 11.84AYY102 pKa = 9.5LTSDD106 pKa = 3.38GAYY109 pKa = 10.24SEE111 pKa = 5.46GLDD114 pKa = 4.7GPLLAQWEE122 pKa = 4.52ADD124 pKa = 3.19STAYY128 pKa = 10.01NACLSGRR135 pKa = 11.84FSADD139 pKa = 2.49EE140 pKa = 3.9ATALRR145 pKa = 11.84QALTFAVGSDD155 pKa = 3.12GNAYY159 pKa = 10.3GLYY162 pKa = 10.54LPGAARR168 pKa = 11.84AIYY171 pKa = 9.83LDD173 pKa = 4.39KK174 pKa = 11.45YY175 pKa = 10.63PIDD178 pKa = 3.74FPFGFEE184 pKa = 3.69LRR186 pKa = 11.84EE187 pKa = 3.84NSRR190 pKa = 11.84SSFRR194 pKa = 11.84AEE196 pKa = 3.84SFGPGGVVEE205 pKa = 4.47SDD207 pKa = 3.41GLQVTYY213 pKa = 8.41LTPEE217 pKa = 4.5DD218 pKa = 4.07GVFSVTTIRR227 pKa = 11.84TVKK230 pKa = 9.55EE231 pKa = 4.01GCGIWGVAVGQPEE244 pKa = 4.07RR245 pKa = 11.84VLLNSSIPLKK255 pKa = 10.81KK256 pKa = 9.28IDD258 pKa = 3.71SLSYY262 pKa = 11.31DD263 pKa = 3.29DD264 pKa = 5.44AAWFGKK270 pKa = 9.7CDD272 pKa = 3.06RR273 pKa = 11.84VYY275 pKa = 11.41AFLPEE280 pKa = 3.98EE281 pKa = 4.04STIAVVFAVKK291 pKa = 10.53DD292 pKa = 3.61DD293 pKa = 3.67VVYY296 pKa = 10.36GIEE299 pKa = 5.32LINGLDD305 pKa = 3.48GQMYY309 pKa = 10.42

Molecular weight: 33.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5UMZ3|A0A1M5UMZ3_9FIRM Putative adhesin OS=Sporobacter termitidis DSM 10068 OX=1123282 GN=SAMN02745823_00594 PE=4 SV=1
MM1 pKa = 7.46ANEE4 pKa = 3.75RR5 pKa = 11.84DD6 pKa = 3.82GRR8 pKa = 11.84DD9 pKa = 3.48GRR11 pKa = 11.84DD12 pKa = 3.39GRR14 pKa = 11.84GHH16 pKa = 4.96GRR18 pKa = 11.84KK19 pKa = 8.98RR20 pKa = 11.84RR21 pKa = 11.84KK22 pKa = 9.04VCQFCVDD29 pKa = 3.12KK30 pKa = 11.13AQNIDD35 pKa = 3.64YY36 pKa = 10.57KK37 pKa = 10.5DD38 pKa = 3.14TAKK41 pKa = 10.09IRR43 pKa = 11.84RR44 pKa = 11.84YY45 pKa = 9.1VSEE48 pKa = 3.99RR49 pKa = 11.84SKK51 pKa = 10.6ILPRR55 pKa = 11.84RR56 pKa = 11.84TTGICAQHH64 pKa = 5.68QRR66 pKa = 11.84QLMVAIKK73 pKa = 10.13RR74 pKa = 11.84ARR76 pKa = 11.84QIALLPYY83 pKa = 9.27VTDD86 pKa = 3.53

Molecular weight: 10.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3771

0

3771

1206611

39

3160

320.0

35.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.791 ± 0.046

1.416 ± 0.017

5.661 ± 0.033

5.942 ± 0.042

4.032 ± 0.028

8.148 ± 0.051

1.604 ± 0.016

6.571 ± 0.034

5.392 ± 0.032

9.643 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.687 ± 0.023

3.692 ± 0.029

4.059 ± 0.026

2.779 ± 0.02

5.086 ± 0.041

6.062 ± 0.038

5.731 ± 0.046

7.234 ± 0.034

0.895 ± 0.013

3.574 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski