Watermelon virus A

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Trivirinae; Wamavirus

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W5W6E2|A0A1W5W6E2_9VIRU Replication-associated polyprotein OS=Watermelon virus A OX=1978413 PE=4 SV=1
MM1 pKa = 7.87EE2 pKa = 6.25DD3 pKa = 4.66LPDD6 pKa = 4.0KK7 pKa = 10.84APRR10 pKa = 11.84DD11 pKa = 3.61ILEE14 pKa = 4.09KK15 pKa = 10.22HH16 pKa = 4.68LHH18 pKa = 5.11EE19 pKa = 4.65RR20 pKa = 11.84EE21 pKa = 4.07CFKK24 pKa = 10.9FLEE27 pKa = 4.21QSFDD31 pKa = 3.69AGDD34 pKa = 3.55KK35 pKa = 10.69GQIPSILVKK44 pKa = 9.52LTRR47 pKa = 11.84VNVGFYY53 pKa = 10.68EE54 pKa = 4.01FLEE57 pKa = 4.48EE58 pKa = 4.54VFTEE62 pKa = 4.2SSISYY67 pKa = 9.86GLSGLEE73 pKa = 4.7KK74 pKa = 10.94GFDD77 pKa = 3.55SLKK80 pKa = 11.17AKK82 pKa = 9.72IDD84 pKa = 3.99KK85 pKa = 10.58INSEE89 pKa = 3.99VSEE92 pKa = 4.31MKK94 pKa = 10.46RR95 pKa = 11.84KK96 pKa = 9.85FEE98 pKa = 3.94NLEE101 pKa = 3.78IKK103 pKa = 10.41LVEE106 pKa = 4.31SQSSEE111 pKa = 4.18EE112 pKa = 4.1EE113 pKa = 4.2GSPDD117 pKa = 3.14VYY119 pKa = 9.55EE120 pKa = 4.51TKK122 pKa = 10.54FSDD125 pKa = 3.41MRR127 pKa = 11.84CDD129 pKa = 3.22RR130 pKa = 11.84KK131 pKa = 9.99GTFLYY136 pKa = 10.31TFEE139 pKa = 4.49GQVNARR145 pKa = 11.84ILSDD149 pKa = 3.75FKK151 pKa = 11.11PFEE154 pKa = 4.43CEE156 pKa = 3.52SGKK159 pKa = 10.5FLVHH163 pKa = 6.87KK164 pKa = 10.69YY165 pKa = 8.1VDD167 pKa = 3.6KK168 pKa = 11.45YY169 pKa = 11.83EE170 pKa = 4.3MIFTHH175 pKa = 7.04LGQVMGYY182 pKa = 9.35FEE184 pKa = 5.56IEE186 pKa = 4.17TNKK189 pKa = 10.34EE190 pKa = 3.9LTFGTLIHH198 pKa = 6.65FLRR201 pKa = 11.84EE202 pKa = 3.78KK203 pKa = 11.13YY204 pKa = 10.64SFNKK208 pKa = 10.25LFIII212 pKa = 5.22

Molecular weight: 24.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W5W6D7|A0A1W5W6D7_9VIRU Coat protein OS=Watermelon virus A OX=1978413 PE=4 SV=1
MM1 pKa = 7.82ASTSMTLVKK10 pKa = 10.53SLEE13 pKa = 3.9KK14 pKa = 10.32RR15 pKa = 11.84IEE17 pKa = 4.06ADD19 pKa = 3.01KK20 pKa = 11.62SLMNSSEE27 pKa = 3.77VDD29 pKa = 3.04KK30 pKa = 11.28LYY32 pKa = 11.42GNLATHH38 pKa = 5.95VFKK41 pKa = 11.34DD42 pKa = 3.64EE43 pKa = 4.1VKK45 pKa = 10.49IKK47 pKa = 10.57IGGNEE52 pKa = 3.91NGNVVSTQCRR62 pKa = 11.84LIGASRR68 pKa = 11.84MQQILNSAQGKK79 pKa = 8.11KK80 pKa = 9.82AAYY83 pKa = 9.56LHH85 pKa = 6.71IGCIPITIQSLLPSGNSKK103 pKa = 10.31IKK105 pKa = 10.29GRR107 pKa = 11.84CALVDD112 pKa = 3.5VSRR115 pKa = 11.84TSLDD119 pKa = 2.94TGLIDD124 pKa = 4.57GFTFSFTDD132 pKa = 3.39EE133 pKa = 4.05KK134 pKa = 10.71PYY136 pKa = 8.56TGRR139 pKa = 11.84MIIVNKK145 pKa = 10.24VIDD148 pKa = 4.22FEE150 pKa = 5.86CDD152 pKa = 2.96ASVGSIQLVLEE163 pKa = 4.25VEE165 pKa = 4.84GIDD168 pKa = 5.37LRR170 pKa = 11.84IQRR173 pKa = 11.84SALAVTVGMTCIPSNTAIVFPSLQRR198 pKa = 11.84SKK200 pKa = 8.34MTWALTNSVDD210 pKa = 4.4MKK212 pKa = 10.01EE213 pKa = 4.08QEE215 pKa = 4.16IKK217 pKa = 10.53EE218 pKa = 4.07DD219 pKa = 3.65EE220 pKa = 4.16QFKK223 pKa = 10.99EE224 pKa = 4.2LFEE227 pKa = 4.24NTNTNLIDD235 pKa = 3.41QGEE238 pKa = 4.46TVLLEE243 pKa = 4.58EE244 pKa = 5.0KK245 pKa = 10.91GSMLNLFGTKK255 pKa = 9.17RR256 pKa = 11.84KK257 pKa = 7.41TVARR261 pKa = 11.84RR262 pKa = 11.84ILKK265 pKa = 10.3NKK267 pKa = 10.09GPICRR272 pKa = 11.84IEE274 pKa = 3.83NWDD277 pKa = 3.51MGSVKK282 pKa = 10.43SGPVKK287 pKa = 10.2MKK289 pKa = 10.36RR290 pKa = 11.84SNSLSSASSFEE301 pKa = 4.35SKK303 pKa = 10.53FDD305 pKa = 3.45GRR307 pKa = 11.84FARR310 pKa = 11.84QSTSGHH316 pKa = 5.08SRR318 pKa = 11.84EE319 pKa = 4.28TSSS322 pKa = 3.91

Molecular weight: 35.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2598

212

1826

649.5

74.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.081 ± 0.929

1.694 ± 0.104

5.504 ± 0.251

9.584 ± 1.075

6.12 ± 0.798

5.312 ± 0.567

2.194 ± 0.338

6.351 ± 0.615

9.623 ± 0.417

9.045 ± 1.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.272 ± 0.194

5.581 ± 0.547

2.348 ± 0.343

2.656 ± 0.385

5.119 ± 0.291

7.121 ± 1.192

4.311 ± 0.769

5.504 ± 0.34

0.962 ± 0.181

2.617 ± 0.566
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski