Amino acid dipepetide frequency for Streptococcus phage K13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.249AlaAla: 2.249 ± 0.865
0.18AlaCys: 0.18 ± 0.125
5.847AlaAsp: 5.847 ± 0.799
6.837AlaGlu: 6.837 ± 0.676
3.059AlaPhe: 3.059 ± 0.834
4.768AlaGly: 4.768 ± 1.311
0.45AlaHis: 0.45 ± 0.181
4.318AlaIle: 4.318 ± 0.978
6.027AlaLys: 6.027 ± 0.846
6.477AlaLeu: 6.477 ± 1.032
2.249AlaMet: 2.249 ± 0.547
3.239AlaAsn: 3.239 ± 0.706
1.889AlaPro: 1.889 ± 0.417
2.789AlaGln: 2.789 ± 0.479
2.609AlaArg: 2.609 ± 0.429
3.059AlaSer: 3.059 ± 0.761
4.588AlaThr: 4.588 ± 1.029
5.668AlaVal: 5.668 ± 0.884
1.529AlaTrp: 1.529 ± 0.52
2.429AlaTyr: 2.429 ± 0.455
0.0AlaXaa: 0.0 ± 0.0
Cys
0.27CysAla: 0.27 ± 0.144
0.09CysCys: 0.09 ± 0.079
0.36CysAsp: 0.36 ± 0.248
0.45CysGlu: 0.45 ± 0.208
0.36CysPhe: 0.36 ± 0.178
0.36CysGly: 0.36 ± 0.354
0.36CysHis: 0.36 ± 0.173
0.45CysIle: 0.45 ± 0.215
0.54CysLys: 0.54 ± 0.151
0.27CysLeu: 0.27 ± 0.148
0.0CysMet: 0.0 ± 0.0
0.27CysAsn: 0.27 ± 0.178
0.36CysPro: 0.36 ± 0.276
0.36CysGln: 0.36 ± 0.159
0.36CysArg: 0.36 ± 0.154
0.09CysSer: 0.09 ± 0.079
0.27CysThr: 0.27 ± 0.164
0.36CysVal: 0.36 ± 0.216
0.18CysTrp: 0.18 ± 0.09
0.36CysTyr: 0.36 ± 0.174
0.0CysXaa: 0.0 ± 0.0
Asp
4.588AspAla: 4.588 ± 0.754
0.63AspCys: 0.63 ± 0.291
3.329AspAsp: 3.329 ± 0.782
5.038AspGlu: 5.038 ± 1.173
3.149AspPhe: 3.149 ± 0.53
4.498AspGly: 4.498 ± 0.577
0.36AspHis: 0.36 ± 0.182
4.048AspIle: 4.048 ± 0.513
4.858AspLys: 4.858 ± 0.759
6.117AspLeu: 6.117 ± 0.882
1.979AspMet: 1.979 ± 0.433
2.879AspAsn: 2.879 ± 0.491
1.619AspPro: 1.619 ± 0.409
1.889AspGln: 1.889 ± 0.568
2.429AspArg: 2.429 ± 0.554
4.048AspSer: 4.048 ± 0.583
3.598AspThr: 3.598 ± 0.455
2.969AspVal: 2.969 ± 0.553
1.439AspTrp: 1.439 ± 0.366
3.418AspTyr: 3.418 ± 0.673
0.0AspXaa: 0.0 ± 0.0
Glu
5.668GluAla: 5.668 ± 0.894
0.54GluCys: 0.54 ± 0.179
4.498GluAsp: 4.498 ± 0.866
6.567GluGlu: 6.567 ± 1.332
3.688GluPhe: 3.688 ± 0.581
3.239GluGly: 3.239 ± 0.595
0.9GluHis: 0.9 ± 0.292
6.297GluIle: 6.297 ± 0.75
6.387GluLys: 6.387 ± 1.186
10.255GluLeu: 10.255 ± 1.222
1.979GluMet: 1.979 ± 0.569
5.128GluAsn: 5.128 ± 0.701
1.439GluPro: 1.439 ± 0.395
4.588GluGln: 4.588 ± 0.926
5.218GluArg: 5.218 ± 0.777
4.588GluSer: 4.588 ± 0.69
3.598GluThr: 3.598 ± 0.623
5.578GluVal: 5.578 ± 0.735
0.81GluTrp: 0.81 ± 0.246
3.059GluTyr: 3.059 ± 0.767
0.0GluXaa: 0.0 ± 0.0
Phe
2.699PheAla: 2.699 ± 0.698
0.18PheCys: 0.18 ± 0.12
4.138PheAsp: 4.138 ± 0.679
4.408PheGlu: 4.408 ± 0.9
1.619PhePhe: 1.619 ± 0.47
2.519PheGly: 2.519 ± 0.825
0.36PheHis: 0.36 ± 0.233
1.979PheIle: 1.979 ± 0.544
3.688PheLys: 3.688 ± 0.673
2.519PheLeu: 2.519 ± 0.494
1.259PheMet: 1.259 ± 0.435
2.519PheAsn: 2.519 ± 0.516
0.72PhePro: 0.72 ± 0.286
1.439PheGln: 1.439 ± 0.42
0.9PheArg: 0.9 ± 0.27
3.688PheSer: 3.688 ± 0.736
2.969PheThr: 2.969 ± 0.518
1.889PheVal: 1.889 ± 0.547
0.54PheTrp: 0.54 ± 0.238
1.619PheTyr: 1.619 ± 0.327
0.0PheXaa: 0.0 ± 0.0
Gly
3.239GlyAla: 3.239 ± 0.707
0.0GlyCys: 0.0 ± 0.0
3.508GlyAsp: 3.508 ± 0.728
4.318GlyGlu: 4.318 ± 0.88
2.609GlyPhe: 2.609 ± 0.736
4.588GlyGly: 4.588 ± 1.533
0.54GlyHis: 0.54 ± 0.174
3.598GlyIle: 3.598 ± 0.677
5.488GlyLys: 5.488 ± 0.685
5.218GlyLeu: 5.218 ± 1.335
1.349GlyMet: 1.349 ± 0.353
3.598GlyAsn: 3.598 ± 0.691
0.99GlyPro: 0.99 ± 0.312
3.329GlyGln: 3.329 ± 0.552
3.329GlyArg: 3.329 ± 0.668
3.868GlySer: 3.868 ± 0.872
2.339GlyThr: 2.339 ± 0.493
5.757GlyVal: 5.757 ± 0.693
0.63GlyTrp: 0.63 ± 0.21
2.879GlyTyr: 2.879 ± 0.582
0.0GlyXaa: 0.0 ± 0.0
His
0.54HisAla: 0.54 ± 0.203
0.0HisCys: 0.0 ± 0.0
0.72HisAsp: 0.72 ± 0.396
1.529HisGlu: 1.529 ± 0.398
0.81HisPhe: 0.81 ± 0.292
1.08HisGly: 1.08 ± 0.412
0.18HisHis: 0.18 ± 0.133
0.36HisIle: 0.36 ± 0.207
0.81HisLys: 0.81 ± 0.358
1.259HisLeu: 1.259 ± 0.329
0.18HisMet: 0.18 ± 0.14
0.63HisAsn: 0.63 ± 0.212
0.72HisPro: 0.72 ± 0.254
0.63HisGln: 0.63 ± 0.323
0.36HisArg: 0.36 ± 0.204
0.81HisSer: 0.81 ± 0.212
0.54HisThr: 0.54 ± 0.237
0.81HisVal: 0.81 ± 0.262
0.18HisTrp: 0.18 ± 0.122
0.36HisTyr: 0.36 ± 0.232
0.0HisXaa: 0.0 ± 0.0
Ile
5.488IleAla: 5.488 ± 0.905
0.45IleCys: 0.45 ± 0.17
2.969IleAsp: 2.969 ± 0.531
6.027IleGlu: 6.027 ± 0.804
3.149IlePhe: 3.149 ± 0.505
3.868IleGly: 3.868 ± 1.034
0.36IleHis: 0.36 ± 0.199
3.418IleIle: 3.418 ± 0.649
6.297IleLys: 6.297 ± 1.009
4.498IleLeu: 4.498 ± 0.737
0.99IleMet: 0.99 ± 0.373
3.688IleAsn: 3.688 ± 0.532
1.349IlePro: 1.349 ± 0.489
2.339IleGln: 2.339 ± 0.389
2.969IleArg: 2.969 ± 0.577
4.408IleSer: 4.408 ± 0.712
4.678IleThr: 4.678 ± 0.612
3.239IleVal: 3.239 ± 0.641
0.18IleTrp: 0.18 ± 0.128
1.619IleTyr: 1.619 ± 0.466
0.0IleXaa: 0.0 ± 0.0
Lys
5.668LysAla: 5.668 ± 0.853
0.54LysCys: 0.54 ± 0.261
5.847LysAsp: 5.847 ± 0.811
8.006LysGlu: 8.006 ± 0.993
2.429LysPhe: 2.429 ± 0.619
4.678LysGly: 4.678 ± 0.661
1.439LysHis: 1.439 ± 0.331
5.398LysIle: 5.398 ± 1.113
7.107LysLys: 7.107 ± 1.284
7.557LysLeu: 7.557 ± 0.854
2.609LysMet: 2.609 ± 0.418
5.308LysAsn: 5.308 ± 0.755
1.979LysPro: 1.979 ± 0.525
3.508LysGln: 3.508 ± 0.719
4.138LysArg: 4.138 ± 0.71
4.498LysSer: 4.498 ± 0.647
4.498LysThr: 4.498 ± 0.542
6.117LysVal: 6.117 ± 0.813
1.169LysTrp: 1.169 ± 0.376
4.318LysTyr: 4.318 ± 0.855
0.0LysXaa: 0.0 ± 0.0
Leu
6.657LeuAla: 6.657 ± 1.016
0.54LeuCys: 0.54 ± 0.26
7.107LeuAsp: 7.107 ± 0.966
7.467LeuGlu: 7.467 ± 1.181
3.059LeuPhe: 3.059 ± 0.631
6.027LeuGly: 6.027 ± 1.744
1.259LeuHis: 1.259 ± 0.381
4.138LeuIle: 4.138 ± 0.475
7.557LeuLys: 7.557 ± 0.955
6.387LeuLeu: 6.387 ± 1.088
1.979LeuMet: 1.979 ± 0.463
3.868LeuAsn: 3.868 ± 0.537
2.159LeuPro: 2.159 ± 0.554
3.688LeuGln: 3.688 ± 0.828
4.048LeuArg: 4.048 ± 0.744
6.207LeuSer: 6.207 ± 0.991
4.768LeuThr: 4.768 ± 0.608
3.329LeuVal: 3.329 ± 0.408
0.63LeuTrp: 0.63 ± 0.22
2.879LeuTyr: 2.879 ± 0.451
0.0LeuXaa: 0.0 ± 0.0
Met
1.799MetAla: 1.799 ± 0.432
0.0MetCys: 0.0 ± 0.0
1.619MetAsp: 1.619 ± 0.378
1.799MetGlu: 1.799 ± 0.441
0.99MetPhe: 0.99 ± 0.317
1.259MetGly: 1.259 ± 0.517
0.27MetHis: 0.27 ± 0.246
1.709MetIle: 1.709 ± 0.423
2.879MetLys: 2.879 ± 0.675
1.799MetLeu: 1.799 ± 0.422
0.45MetMet: 0.45 ± 0.298
1.709MetAsn: 1.709 ± 0.474
0.72MetPro: 0.72 ± 0.306
0.99MetGln: 0.99 ± 0.515
0.9MetArg: 0.9 ± 0.325
0.72MetSer: 0.72 ± 0.246
1.979MetThr: 1.979 ± 0.511
1.08MetVal: 1.08 ± 0.328
0.36MetTrp: 0.36 ± 0.238
0.63MetTyr: 0.63 ± 0.194
0.0MetXaa: 0.0 ± 0.0
Asn
4.948AsnAla: 4.948 ± 1.149
0.45AsnCys: 0.45 ± 0.205
3.239AsnAsp: 3.239 ± 0.579
3.598AsnGlu: 3.598 ± 0.67
2.159AsnPhe: 2.159 ± 0.603
3.329AsnGly: 3.329 ± 0.584
1.169AsnHis: 1.169 ± 0.346
2.969AsnIle: 2.969 ± 0.509
4.318AsnLys: 4.318 ± 0.55
4.678AsnLeu: 4.678 ± 0.756
1.08AsnMet: 1.08 ± 0.386
2.519AsnAsn: 2.519 ± 0.605
1.799AsnPro: 1.799 ± 0.405
2.609AsnGln: 2.609 ± 0.506
2.609AsnArg: 2.609 ± 0.495
2.879AsnSer: 2.879 ± 0.716
3.508AsnThr: 3.508 ± 0.663
3.688AsnVal: 3.688 ± 0.51
1.08AsnTrp: 1.08 ± 0.253
1.799AsnTyr: 1.799 ± 0.362
0.0AsnXaa: 0.0 ± 0.0
Pro
2.159ProAla: 2.159 ± 0.505
0.09ProCys: 0.09 ± 0.118
1.439ProAsp: 1.439 ± 0.335
2.879ProGlu: 2.879 ± 0.458
0.99ProPhe: 0.99 ± 0.367
1.08ProGly: 1.08 ± 0.305
0.27ProHis: 0.27 ± 0.142
1.439ProIle: 1.439 ± 0.509
2.519ProLys: 2.519 ± 0.449
1.349ProLeu: 1.349 ± 0.379
0.36ProMet: 0.36 ± 0.186
0.81ProAsn: 0.81 ± 0.3
0.63ProPro: 0.63 ± 0.313
1.08ProGln: 1.08 ± 0.352
1.08ProArg: 1.08 ± 0.275
0.9ProSer: 0.9 ± 0.437
0.9ProThr: 0.9 ± 0.302
1.619ProVal: 1.619 ± 0.313
0.63ProTrp: 0.63 ± 0.249
1.259ProTyr: 1.259 ± 0.437
0.0ProXaa: 0.0 ± 0.0
Gln
3.778GlnAla: 3.778 ± 0.589
0.45GlnCys: 0.45 ± 0.226
2.339GlnAsp: 2.339 ± 0.386
4.498GlnGlu: 4.498 ± 0.878
0.9GlnPhe: 0.9 ± 0.3
2.159GlnGly: 2.159 ± 0.418
0.27GlnHis: 0.27 ± 0.157
3.418GlnIle: 3.418 ± 0.517
4.048GlnLys: 4.048 ± 0.65
3.059GlnLeu: 3.059 ± 0.483
0.99GlnMet: 0.99 ± 0.256
2.069GlnAsn: 2.069 ± 0.445
0.81GlnPro: 0.81 ± 0.262
1.799GlnGln: 1.799 ± 0.412
2.159GlnArg: 2.159 ± 0.484
3.329GlnSer: 3.329 ± 0.527
2.879GlnThr: 2.879 ± 0.639
3.688GlnVal: 3.688 ± 0.636
0.36GlnTrp: 0.36 ± 0.163
0.63GlnTyr: 0.63 ± 0.233
0.0GlnXaa: 0.0 ± 0.0
Arg
3.418ArgAla: 3.418 ± 0.541
0.36ArgCys: 0.36 ± 0.151
2.429ArgAsp: 2.429 ± 0.492
2.789ArgGlu: 2.789 ± 0.572
2.159ArgPhe: 2.159 ± 0.558
1.799ArgGly: 1.799 ± 0.515
0.54ArgHis: 0.54 ± 0.221
3.059ArgIle: 3.059 ± 0.642
3.418ArgLys: 3.418 ± 0.791
4.948ArgLeu: 4.948 ± 0.841
2.069ArgMet: 2.069 ± 0.505
2.339ArgAsn: 2.339 ± 0.673
0.81ArgPro: 0.81 ± 0.263
2.789ArgGln: 2.789 ± 0.54
1.889ArgArg: 1.889 ± 0.45
2.339ArgSer: 2.339 ± 0.489
3.329ArgThr: 3.329 ± 0.839
2.789ArgVal: 2.789 ± 0.424
0.27ArgTrp: 0.27 ± 0.182
1.979ArgTyr: 1.979 ± 0.454
0.0ArgXaa: 0.0 ± 0.0
Ser
5.038SerAla: 5.038 ± 1.203
0.18SerCys: 0.18 ± 0.117
3.508SerAsp: 3.508 ± 0.699
4.318SerGlu: 4.318 ± 0.707
1.979SerPhe: 1.979 ± 0.425
4.678SerGly: 4.678 ± 0.841
1.08SerHis: 1.08 ± 0.403
4.048SerIle: 4.048 ± 0.695
5.308SerLys: 5.308 ± 0.784
5.038SerLeu: 5.038 ± 0.71
1.439SerMet: 1.439 ± 0.557
3.868SerAsn: 3.868 ± 0.708
1.08SerPro: 1.08 ± 0.289
2.069SerGln: 2.069 ± 0.484
3.778SerArg: 3.778 ± 0.882
4.048SerSer: 4.048 ± 0.802
3.418SerThr: 3.418 ± 0.454
3.598SerVal: 3.598 ± 0.753
0.63SerTrp: 0.63 ± 0.432
2.249SerTyr: 2.249 ± 0.758
0.0SerXaa: 0.0 ± 0.0
Thr
5.038ThrAla: 5.038 ± 0.991
0.18ThrCys: 0.18 ± 0.137
3.958ThrAsp: 3.958 ± 0.639
3.688ThrGlu: 3.688 ± 0.621
3.149ThrPhe: 3.149 ± 0.786
3.868ThrGly: 3.868 ± 0.657
0.9ThrHis: 0.9 ± 0.321
4.318ThrIle: 4.318 ± 0.597
4.588ThrLys: 4.588 ± 0.766
4.048ThrLeu: 4.048 ± 0.594
0.54ThrMet: 0.54 ± 0.261
3.598ThrAsn: 3.598 ± 0.522
0.99ThrPro: 0.99 ± 0.332
2.969ThrGln: 2.969 ± 0.711
1.529ThrArg: 1.529 ± 0.405
4.048ThrSer: 4.048 ± 0.577
4.048ThrThr: 4.048 ± 0.803
5.038ThrVal: 5.038 ± 0.818
0.72ThrTrp: 0.72 ± 0.263
2.339ThrTyr: 2.339 ± 0.552
0.0ThrXaa: 0.0 ± 0.0
Val
4.498ValAla: 4.498 ± 0.644
0.27ValCys: 0.27 ± 0.171
3.329ValAsp: 3.329 ± 0.62
6.297ValGlu: 6.297 ± 0.834
2.249ValPhe: 2.249 ± 0.531
4.768ValGly: 4.768 ± 0.946
0.72ValHis: 0.72 ± 0.266
3.149ValIle: 3.149 ± 0.518
5.668ValLys: 5.668 ± 0.633
4.858ValLeu: 4.858 ± 0.816
1.08ValMet: 1.08 ± 0.413
3.688ValAsn: 3.688 ± 0.896
1.889ValPro: 1.889 ± 0.355
1.799ValGln: 1.799 ± 0.499
2.789ValArg: 2.789 ± 0.423
5.038ValSer: 5.038 ± 0.832
4.948ValThr: 4.948 ± 0.734
4.678ValVal: 4.678 ± 0.964
0.63ValTrp: 0.63 ± 0.22
3.508ValTyr: 3.508 ± 0.661
0.0ValXaa: 0.0 ± 0.0
Trp
1.259TrpAla: 1.259 ± 0.402
0.27TrpCys: 0.27 ± 0.156
0.45TrpAsp: 0.45 ± 0.363
0.72TrpGlu: 0.72 ± 0.323
0.99TrpPhe: 0.99 ± 0.539
0.54TrpGly: 0.54 ± 0.205
0.09TrpHis: 0.09 ± 0.129
0.72TrpIle: 0.72 ± 0.273
1.349TrpLys: 1.349 ± 0.383
0.63TrpLeu: 0.63 ± 0.27
0.27TrpMet: 0.27 ± 0.147
0.99TrpAsn: 0.99 ± 0.33
0.09TrpPro: 0.09 ± 0.096
0.63TrpGln: 0.63 ± 0.369
0.63TrpArg: 0.63 ± 0.26
0.54TrpSer: 0.54 ± 0.209
0.72TrpThr: 0.72 ± 0.322
1.169TrpVal: 1.169 ± 0.359
0.18TrpTrp: 0.18 ± 0.108
0.27TrpTyr: 0.27 ± 0.11
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.259TyrAla: 1.259 ± 0.319
0.72TyrCys: 0.72 ± 0.356
2.249TyrAsp: 2.249 ± 0.481
2.789TyrGlu: 2.789 ± 0.543
2.159TyrPhe: 2.159 ± 0.618
2.069TyrGly: 2.069 ± 0.393
0.99TyrHis: 0.99 ± 0.29
3.149TyrIle: 3.149 ± 0.655
3.958TyrLys: 3.958 ± 0.786
2.879TyrLeu: 2.879 ± 0.669
0.72TyrMet: 0.72 ± 0.449
1.709TyrAsn: 1.709 ± 0.332
1.439TyrPro: 1.439 ± 0.382
2.339TyrGln: 2.339 ± 0.475
1.799TyrArg: 1.799 ± 0.475
2.249TyrSer: 2.249 ± 0.497
1.979TyrThr: 1.979 ± 0.499
2.789TyrVal: 2.789 ± 0.596
0.36TyrTrp: 0.36 ± 0.174
1.259TyrTyr: 1.259 ± 0.393
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (11117 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski