Sophora alopecuroides yellow stunt alphasatellite 1a
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W5YSH5|A0A1W5YSH5_9VIRU Satellite replication initiator protein OS=Sophora alopecuroides yellow stunt alphasatellite 1a OX=1980161 PE=4 SV=1
MM1 pKa = 7.71 APTRR5 pKa = 11.84 YY6 pKa = 9.93 CFTLNYY12 pKa = 8.75 QTDD15 pKa = 4.07 LEE17 pKa = 4.43 RR18 pKa = 11.84 EE19 pKa = 4.04 NFLSLFSRR27 pKa = 11.84 DD28 pKa = 3.48 EE29 pKa = 3.8 INYY32 pKa = 9.68 LVVGDD37 pKa = 4.54 EE38 pKa = 4.53 VAGTGQKK45 pKa = 10.02 HH46 pKa = 4.43 LQGYY50 pKa = 9.32 VSFKK54 pKa = 10.05 NQIRR58 pKa = 11.84 LGGLKK63 pKa = 9.7 KK64 pKa = 10.39 KK65 pKa = 10.8 YY66 pKa = 8.78 GARR69 pKa = 11.84 VHH71 pKa = 6.18 WEE73 pKa = 3.47 IARR76 pKa = 11.84 GDD78 pKa = 3.6 DD79 pKa = 3.53 SQNRR83 pKa = 11.84 DD84 pKa = 3.28 YY85 pKa = 11.17 CSKK88 pKa = 10.86 EE89 pKa = 3.66 NLILEE94 pKa = 4.2 IGVPVKK100 pKa = 10.58 KK101 pKa = 10.51 GSCRR105 pKa = 11.84 RR106 pKa = 11.84 KK107 pKa = 9.63 IMQLYY112 pKa = 9.91 EE113 pKa = 4.16 EE114 pKa = 5.42 DD115 pKa = 4.04 PEE117 pKa = 4.29 EE118 pKa = 4.21 MQLKK122 pKa = 10.83 DD123 pKa = 3.36 PDD125 pKa = 3.52 TALRR129 pKa = 11.84 CKK131 pKa = 10.33 AKK133 pKa = 10.35 KK134 pKa = 10.26 LKK136 pKa = 10.18 EE137 pKa = 4.38 DD138 pKa = 3.72 YY139 pKa = 10.47 CSSFSSYY146 pKa = 7.35 QLRR149 pKa = 11.84 PWQIKK154 pKa = 8.49 LHH156 pKa = 4.98 QKK158 pKa = 9.88 ILEE161 pKa = 4.39 EE162 pKa = 4.77 PDD164 pKa = 3.28 DD165 pKa = 3.77 RR166 pKa = 11.84 TIIWVYY172 pKa = 10.79 GPDD175 pKa = 3.46 GGEE178 pKa = 4.18 GKK180 pKa = 8.39 STFAKK185 pKa = 10.41 EE186 pKa = 3.8 LIKK189 pKa = 11.03 YY190 pKa = 8.49 GWFYY194 pKa = 11.04 TSGGKK199 pKa = 7.39 TQDD202 pKa = 3.02 ILYY205 pKa = 8.78 MYY207 pKa = 10.51 AQDD210 pKa = 3.86 PEE212 pKa = 4.49 RR213 pKa = 11.84 NIAFDD218 pKa = 3.62 VPRR221 pKa = 11.84 CSSEE225 pKa = 3.61 MMNYY229 pKa = 9.68 QVMEE233 pKa = 4.05 MMKK236 pKa = 9.86 NRR238 pKa = 11.84 CFASTKK244 pKa = 9.65 YY245 pKa = 10.38 RR246 pKa = 11.84 PVDD249 pKa = 3.18 LCVRR253 pKa = 11.84 KK254 pKa = 9.76 KK255 pKa = 10.35 CHH257 pKa = 6.76 LIVFANVAPDD267 pKa = 3.52 PTKK270 pKa = 10.29 ISEE273 pKa = 4.03 DD274 pKa = 3.73 RR275 pKa = 11.84 IVIIDD280 pKa = 3.51 TT281 pKa = 3.77
Molecular weight: 32.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.96
IPC2_protein 7.307
IPC_protein 7.205
Toseland 6.781
ProMoST 7.717
Dawson 7.892
Bjellqvist 8.244
Wikipedia 7.805
Rodwell 7.907
Grimsley 6.795
Solomon 7.966
Lehninger 7.98
Nozaki 8.463
DTASelect 8.009
Thurlkill 8.068
EMBOSS 8.112
Sillero 8.39
Patrickios 4.139
IPC_peptide 7.966
IPC2_peptide 7.307
IPC2.peptide.svr19 7.434
Protein with the highest isoelectric point:
>tr|A0A1W5YSH5|A0A1W5YSH5_9VIRU Satellite replication initiator protein OS=Sophora alopecuroides yellow stunt alphasatellite 1a OX=1980161 PE=4 SV=1
MM1 pKa = 7.71 APTRR5 pKa = 11.84 YY6 pKa = 9.93 CFTLNYY12 pKa = 8.75 QTDD15 pKa = 4.07 LEE17 pKa = 4.43 RR18 pKa = 11.84 EE19 pKa = 4.04 NFLSLFSRR27 pKa = 11.84 DD28 pKa = 3.48 EE29 pKa = 3.8 INYY32 pKa = 9.68 LVVGDD37 pKa = 4.54 EE38 pKa = 4.53 VAGTGQKK45 pKa = 10.02 HH46 pKa = 4.43 LQGYY50 pKa = 9.32 VSFKK54 pKa = 10.05 NQIRR58 pKa = 11.84 LGGLKK63 pKa = 9.7 KK64 pKa = 10.39 KK65 pKa = 10.8 YY66 pKa = 8.78 GARR69 pKa = 11.84 VHH71 pKa = 6.18 WEE73 pKa = 3.47 IARR76 pKa = 11.84 GDD78 pKa = 3.6 DD79 pKa = 3.53 SQNRR83 pKa = 11.84 DD84 pKa = 3.28 YY85 pKa = 11.17 CSKK88 pKa = 10.86 EE89 pKa = 3.66 NLILEE94 pKa = 4.2 IGVPVKK100 pKa = 10.58 KK101 pKa = 10.51 GSCRR105 pKa = 11.84 RR106 pKa = 11.84 KK107 pKa = 9.63 IMQLYY112 pKa = 9.91 EE113 pKa = 4.16 EE114 pKa = 5.42 DD115 pKa = 4.04 PEE117 pKa = 4.29 EE118 pKa = 4.21 MQLKK122 pKa = 10.83 DD123 pKa = 3.36 PDD125 pKa = 3.52 TALRR129 pKa = 11.84 CKK131 pKa = 10.33 AKK133 pKa = 10.35 KK134 pKa = 10.26 LKK136 pKa = 10.18 EE137 pKa = 4.38 DD138 pKa = 3.72 YY139 pKa = 10.47 CSSFSSYY146 pKa = 7.35 QLRR149 pKa = 11.84 PWQIKK154 pKa = 8.49 LHH156 pKa = 4.98 QKK158 pKa = 9.88 ILEE161 pKa = 4.39 EE162 pKa = 4.77 PDD164 pKa = 3.28 DD165 pKa = 3.77 RR166 pKa = 11.84 TIIWVYY172 pKa = 10.79 GPDD175 pKa = 3.46 GGEE178 pKa = 4.18 GKK180 pKa = 8.39 STFAKK185 pKa = 10.41 EE186 pKa = 3.8 LIKK189 pKa = 11.03 YY190 pKa = 8.49 GWFYY194 pKa = 11.04 TSGGKK199 pKa = 7.39 TQDD202 pKa = 3.02 ILYY205 pKa = 8.78 MYY207 pKa = 10.51 AQDD210 pKa = 3.86 PEE212 pKa = 4.49 RR213 pKa = 11.84 NIAFDD218 pKa = 3.62 VPRR221 pKa = 11.84 CSSEE225 pKa = 3.61 MMNYY229 pKa = 9.68 QVMEE233 pKa = 4.05 MMKK236 pKa = 9.86 NRR238 pKa = 11.84 CFASTKK244 pKa = 9.65 YY245 pKa = 10.38 RR246 pKa = 11.84 PVDD249 pKa = 3.18 LCVRR253 pKa = 11.84 KK254 pKa = 9.76 KK255 pKa = 10.35 CHH257 pKa = 6.76 LIVFANVAPDD267 pKa = 3.52 PTKK270 pKa = 10.29 ISEE273 pKa = 4.03 DD274 pKa = 3.73 RR275 pKa = 11.84 IVIIDD280 pKa = 3.51 TT281 pKa = 3.77
Molecular weight: 32.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.96
IPC2_protein 7.307
IPC_protein 7.205
Toseland 6.781
ProMoST 7.717
Dawson 7.892
Bjellqvist 8.244
Wikipedia 7.805
Rodwell 7.907
Grimsley 6.795
Solomon 7.966
Lehninger 7.98
Nozaki 8.463
DTASelect 8.009
Thurlkill 8.068
EMBOSS 8.112
Sillero 8.39
Patrickios 4.139
IPC_peptide 7.966
IPC2_peptide 7.307
IPC2.peptide.svr19 7.434
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
281
281
281
281.0
32.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.27 ± 0.0
3.203 ± 0.0
7.117 ± 0.0
7.117 ± 0.0
3.559 ± 0.0
6.05 ± 0.0
1.423 ± 0.0
6.406 ± 0.0
8.897 ± 0.0
7.473 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.203 ± 0.0
3.559 ± 0.0
4.27 ± 0.0
4.626 ± 0.0
6.406 ± 0.0
5.694 ± 0.0
4.27 ± 0.0
5.338 ± 0.0
1.423 ± 0.0
5.694 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here