Actinidia seed borne latent virus
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2L0V1P5|A0A2L0V1P5_9VIRU Nucleic acid binding protein OS=Actinidia seed borne latent virus OX=2560282 PE=4 SV=1
MM1 pKa = 7.37 SKK3 pKa = 10.24 SIKK6 pKa = 10.21 VNSIVNRR13 pKa = 11.84 VNVDD17 pKa = 3.12 KK18 pKa = 11.18 SILGSSEE25 pKa = 3.36 ISSLYY30 pKa = 8.58 GSKK33 pKa = 10.34 YY34 pKa = 10.37 APLVFKK40 pKa = 10.98 DD41 pKa = 4.01 EE42 pKa = 4.14 VKK44 pKa = 10.45 MIVPGNILGGPIKK57 pKa = 10.51 LQANILTKK65 pKa = 10.42 EE66 pKa = 3.67 RR67 pKa = 11.84 LGIIRR72 pKa = 11.84 GQKK75 pKa = 10.24 FGGSNCAYY83 pKa = 9.69 LHH85 pKa = 6.49 LGFVPIVIQSLLVSGNEE102 pKa = 4.01 LVKK105 pKa = 10.74 GRR107 pKa = 11.84 CSLVDD112 pKa = 3.82 LSRR115 pKa = 11.84 GSEE118 pKa = 3.86 KK119 pKa = 10.3 TGLIDD124 pKa = 3.85 RR125 pKa = 11.84 FNFKK129 pKa = 8.67 FTKK132 pKa = 10.43 NEE134 pKa = 3.49 PFAAKK139 pKa = 9.83 ILTINAPIDD148 pKa = 3.77 INCDD152 pKa = 3.02 TSINSIQILLEE163 pKa = 3.92 LEE165 pKa = 5.07 GIDD168 pKa = 3.36 IRR170 pKa = 11.84 TEE172 pKa = 3.72 RR173 pKa = 11.84 SVIAVVTGLSCVPTNSTIMLPGLKK197 pKa = 9.67 RR198 pKa = 11.84 EE199 pKa = 4.1 TPKK202 pKa = 10.5 WSICNVFNVPEE213 pKa = 4.03 EE214 pKa = 4.25 SEE216 pKa = 4.07 EE217 pKa = 4.04 DD218 pKa = 3.41 NEE220 pKa = 4.82 RR221 pKa = 11.84 FNSLFNGANPKK232 pKa = 10.56 LIDD235 pKa = 3.99 LGKK238 pKa = 9.55 DD239 pKa = 3.1 TVLDD243 pKa = 3.65 NGKK246 pKa = 9.84 KK247 pKa = 9.91 FGFWGPSVKK256 pKa = 9.7 PVHH259 pKa = 6.55 RR260 pKa = 11.84 RR261 pKa = 11.84 EE262 pKa = 4.15 LTTKK266 pKa = 10.76 NIIKK270 pKa = 9.64 EE271 pKa = 3.98 QMSQVMSEE279 pKa = 4.33 TVSNLNICEE288 pKa = 4.0 EE289 pKa = 4.13 RR290 pKa = 11.84 KK291 pKa = 10.0 KK292 pKa = 10.9 LRR294 pKa = 11.84 NCLEE298 pKa = 3.71 RR299 pKa = 11.84 SKK301 pKa = 10.92 SVRR304 pKa = 11.84 GNVSEE309 pKa = 4.22 VGKK312 pKa = 10.84 EE313 pKa = 3.78 EE314 pKa = 4.25 VPVRR318 pKa = 11.84 RR319 pKa = 11.84 SLRR322 pKa = 11.84 VDD324 pKa = 3.03 MEE326 pKa = 4.04 EE327 pKa = 3.85 LHH329 pKa = 7.4 RR330 pKa = 11.84 SGRR333 pKa = 11.84 QDD335 pKa = 2.64 ARR337 pKa = 11.84 MEE339 pKa = 4.59 GGQQWEE345 pKa = 4.82 CEE347 pKa = 3.88 ASKK350 pKa = 10.82 YY351 pKa = 10.13 KK352 pKa = 10.51 HH353 pKa = 6.96 DD354 pKa = 3.66 GTEE357 pKa = 3.78 VKK359 pKa = 10.42 DD360 pKa = 3.79 YY361 pKa = 11.27 DD362 pKa = 3.9 GSAEE366 pKa = 3.94 SDD368 pKa = 2.71 IRR370 pKa = 11.84 EE371 pKa = 4.08 YY372 pKa = 11.14 CRR374 pKa = 11.84 LLGGSRR380 pKa = 11.84 NKK382 pKa = 10.66 LSIDD386 pKa = 3.63 YY387 pKa = 10.98 SGLQCDD393 pKa = 3.3 EE394 pKa = 5.11 CEE396 pKa = 4.04 WDD398 pKa = 3.57 RR399 pKa = 11.84 KK400 pKa = 10.33 SDD402 pKa = 3.23 INRR405 pKa = 11.84 WEE407 pKa = 4.06 FQSEE411 pKa = 4.25 DD412 pKa = 3.3 NSGNNEE418 pKa = 4.22 DD419 pKa = 4.13 LHH421 pKa = 7.29 SNPSGSEE428 pKa = 4.0 DD429 pKa = 3.6 IIINAKK435 pKa = 10.36 GGLQEE440 pKa = 4.25 FCEE443 pKa = 4.33 LRR445 pKa = 11.84 PP446 pKa = 3.63
Molecular weight: 49.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.787
IPC2_protein 5.893
IPC_protein 5.893
Toseland 5.982
ProMoST 6.186
Dawson 6.084
Bjellqvist 6.122
Wikipedia 6.046
Rodwell 6.046
Grimsley 6.008
Solomon 6.084
Lehninger 6.059
Nozaki 6.313
DTASelect 6.465
Thurlkill 6.427
EMBOSS 6.389
Sillero 6.415
Patrickios 4.495
IPC_peptide 6.097
IPC2_peptide 6.326
IPC2.peptide.svr19 6.479
Protein with the highest isoelectric point:
>tr|A0A2L0V1P9|A0A2L0V1P9_9VIRU Coat protein OS=Actinidia seed borne latent virus OX=2560282 PE=4 SV=1
MM1 pKa = 7.46 EE2 pKa = 5.16 KK3 pKa = 10.75 AEE5 pKa = 4.21 SFAGKK10 pKa = 8.31 TIKK13 pKa = 10.51 NWVALNKK20 pKa = 10.55 ANIFLSLINSFGRR33 pKa = 11.84 DD34 pKa = 2.65 IGFKK38 pKa = 9.99 IFMMYY43 pKa = 9.79 KK44 pKa = 9.5 CKK46 pKa = 10.46 CEE48 pKa = 4.4 KK49 pKa = 10.17 EE50 pKa = 4.16 YY51 pKa = 11.06 KK52 pKa = 10.41 DD53 pKa = 3.43 QIRR56 pKa = 11.84 YY57 pKa = 9.22 QNMVNYY63 pKa = 9.66 GQGKK67 pKa = 9.3 SKK69 pKa = 11.0 SAMKK73 pKa = 10.2 RR74 pKa = 11.84 RR75 pKa = 11.84 AIKK78 pKa = 10.45 VEE80 pKa = 3.61 HH81 pKa = 6.99 CYY83 pKa = 10.69 KK84 pKa = 10.46 CGKK87 pKa = 9.69 FEE89 pKa = 6.07 HH90 pKa = 7.43 DD91 pKa = 3.35 GHH93 pKa = 6.46 CNKK96 pKa = 10.0 NQTNSNHH103 pKa = 6.58 EE104 pKa = 4.09 YY105 pKa = 11.23 LEE107 pKa = 3.98 MFRR110 pKa = 11.84 CGPIKK115 pKa = 10.58 LKK117 pKa = 10.9 AEE119 pKa = 3.63 RR120 pKa = 11.84 ALRR123 pKa = 11.84 KK124 pKa = 10.1 NSMVQMSCEE133 pKa = 3.75 KK134 pKa = 9.83 FGWMIKK140 pKa = 8.78 MSKK143 pKa = 8.48 EE144 pKa = 3.97 LKK146 pKa = 9.94 EE147 pKa = 4.05 KK148 pKa = 10.78 ANSGTAEE155 pKa = 3.98 RR156 pKa = 11.84 VV157 pKa = 3.17
Molecular weight: 18.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.96
IPC2_protein 8.96
IPC_protein 8.829
Toseland 9.794
ProMoST 9.399
Dawson 9.97
Bjellqvist 9.648
Wikipedia 10.087
Rodwell 10.584
Grimsley 10.014
Solomon 9.999
Lehninger 9.984
Nozaki 9.911
DTASelect 9.604
Thurlkill 9.853
EMBOSS 10.204
Sillero 9.926
Patrickios 10.204
IPC_peptide 9.999
IPC2_peptide 8.317
IPC2.peptide.svr19 7.837
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2827
157
2002
706.8
81.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.174 ± 0.816
2.335 ± 0.535
5.766 ± 0.805
8.56 ± 0.648
5.624 ± 0.904
5.341 ± 0.929
2.299 ± 0.47
6.969 ± 0.344
9.268 ± 1.519
7.853 ± 0.672
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.042 ± 0.782
6.155 ± 0.748
2.688 ± 0.452
2.618 ± 0.267
5.624 ± 0.426
7.676 ± 0.804
3.714 ± 0.662
6.19 ± 0.587
1.097 ± 0.074
3.007 ± 0.514
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here