Actinidia seed borne latent virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Trivirinae; Prunevirus

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2L0V1P5|A0A2L0V1P5_9VIRU Nucleic acid binding protein OS=Actinidia seed borne latent virus OX=2560282 PE=4 SV=1
MM1 pKa = 7.37SKK3 pKa = 10.24SIKK6 pKa = 10.21VNSIVNRR13 pKa = 11.84VNVDD17 pKa = 3.12KK18 pKa = 11.18SILGSSEE25 pKa = 3.36ISSLYY30 pKa = 8.58GSKK33 pKa = 10.34YY34 pKa = 10.37APLVFKK40 pKa = 10.98DD41 pKa = 4.01EE42 pKa = 4.14VKK44 pKa = 10.45MIVPGNILGGPIKK57 pKa = 10.51LQANILTKK65 pKa = 10.42EE66 pKa = 3.67RR67 pKa = 11.84LGIIRR72 pKa = 11.84GQKK75 pKa = 10.24FGGSNCAYY83 pKa = 9.69LHH85 pKa = 6.49LGFVPIVIQSLLVSGNEE102 pKa = 4.01LVKK105 pKa = 10.74GRR107 pKa = 11.84CSLVDD112 pKa = 3.82LSRR115 pKa = 11.84GSEE118 pKa = 3.86KK119 pKa = 10.3TGLIDD124 pKa = 3.85RR125 pKa = 11.84FNFKK129 pKa = 8.67FTKK132 pKa = 10.43NEE134 pKa = 3.49PFAAKK139 pKa = 9.83ILTINAPIDD148 pKa = 3.77INCDD152 pKa = 3.02TSINSIQILLEE163 pKa = 3.92LEE165 pKa = 5.07GIDD168 pKa = 3.36IRR170 pKa = 11.84TEE172 pKa = 3.72RR173 pKa = 11.84SVIAVVTGLSCVPTNSTIMLPGLKK197 pKa = 9.67RR198 pKa = 11.84EE199 pKa = 4.1TPKK202 pKa = 10.5WSICNVFNVPEE213 pKa = 4.03EE214 pKa = 4.25SEE216 pKa = 4.07EE217 pKa = 4.04DD218 pKa = 3.41NEE220 pKa = 4.82RR221 pKa = 11.84FNSLFNGANPKK232 pKa = 10.56LIDD235 pKa = 3.99LGKK238 pKa = 9.55DD239 pKa = 3.1TVLDD243 pKa = 3.65NGKK246 pKa = 9.84KK247 pKa = 9.91FGFWGPSVKK256 pKa = 9.7PVHH259 pKa = 6.55RR260 pKa = 11.84RR261 pKa = 11.84EE262 pKa = 4.15LTTKK266 pKa = 10.76NIIKK270 pKa = 9.64EE271 pKa = 3.98QMSQVMSEE279 pKa = 4.33TVSNLNICEE288 pKa = 4.0EE289 pKa = 4.13RR290 pKa = 11.84KK291 pKa = 10.0KK292 pKa = 10.9LRR294 pKa = 11.84NCLEE298 pKa = 3.71RR299 pKa = 11.84SKK301 pKa = 10.92SVRR304 pKa = 11.84GNVSEE309 pKa = 4.22VGKK312 pKa = 10.84EE313 pKa = 3.78EE314 pKa = 4.25VPVRR318 pKa = 11.84RR319 pKa = 11.84SLRR322 pKa = 11.84VDD324 pKa = 3.03MEE326 pKa = 4.04EE327 pKa = 3.85LHH329 pKa = 7.4RR330 pKa = 11.84SGRR333 pKa = 11.84QDD335 pKa = 2.64ARR337 pKa = 11.84MEE339 pKa = 4.59GGQQWEE345 pKa = 4.82CEE347 pKa = 3.88ASKK350 pKa = 10.82YY351 pKa = 10.13KK352 pKa = 10.51HH353 pKa = 6.96DD354 pKa = 3.66GTEE357 pKa = 3.78VKK359 pKa = 10.42DD360 pKa = 3.79YY361 pKa = 11.27DD362 pKa = 3.9GSAEE366 pKa = 3.94SDD368 pKa = 2.71IRR370 pKa = 11.84EE371 pKa = 4.08YY372 pKa = 11.14CRR374 pKa = 11.84LLGGSRR380 pKa = 11.84NKK382 pKa = 10.66LSIDD386 pKa = 3.63YY387 pKa = 10.98SGLQCDD393 pKa = 3.3EE394 pKa = 5.11CEE396 pKa = 4.04WDD398 pKa = 3.57RR399 pKa = 11.84KK400 pKa = 10.33SDD402 pKa = 3.23INRR405 pKa = 11.84WEE407 pKa = 4.06FQSEE411 pKa = 4.25DD412 pKa = 3.3NSGNNEE418 pKa = 4.22DD419 pKa = 4.13LHH421 pKa = 7.29SNPSGSEE428 pKa = 4.0DD429 pKa = 3.6IIINAKK435 pKa = 10.36GGLQEE440 pKa = 4.25FCEE443 pKa = 4.33LRR445 pKa = 11.84PP446 pKa = 3.63

Molecular weight:
49.87 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2L0V1P9|A0A2L0V1P9_9VIRU Coat protein OS=Actinidia seed borne latent virus OX=2560282 PE=4 SV=1
MM1 pKa = 7.46EE2 pKa = 5.16KK3 pKa = 10.75AEE5 pKa = 4.21SFAGKK10 pKa = 8.31TIKK13 pKa = 10.51NWVALNKK20 pKa = 10.55ANIFLSLINSFGRR33 pKa = 11.84DD34 pKa = 2.65IGFKK38 pKa = 9.99IFMMYY43 pKa = 9.79KK44 pKa = 9.5CKK46 pKa = 10.46CEE48 pKa = 4.4KK49 pKa = 10.17EE50 pKa = 4.16YY51 pKa = 11.06KK52 pKa = 10.41DD53 pKa = 3.43QIRR56 pKa = 11.84YY57 pKa = 9.22QNMVNYY63 pKa = 9.66GQGKK67 pKa = 9.3SKK69 pKa = 11.0SAMKK73 pKa = 10.2RR74 pKa = 11.84RR75 pKa = 11.84AIKK78 pKa = 10.45VEE80 pKa = 3.61HH81 pKa = 6.99CYY83 pKa = 10.69KK84 pKa = 10.46CGKK87 pKa = 9.69FEE89 pKa = 6.07HH90 pKa = 7.43DD91 pKa = 3.35GHH93 pKa = 6.46CNKK96 pKa = 10.0NQTNSNHH103 pKa = 6.58EE104 pKa = 4.09YY105 pKa = 11.23LEE107 pKa = 3.98MFRR110 pKa = 11.84CGPIKK115 pKa = 10.58LKK117 pKa = 10.9AEE119 pKa = 3.63RR120 pKa = 11.84ALRR123 pKa = 11.84KK124 pKa = 10.1NSMVQMSCEE133 pKa = 3.75KK134 pKa = 9.83FGWMIKK140 pKa = 8.78MSKK143 pKa = 8.48EE144 pKa = 3.97LKK146 pKa = 9.94EE147 pKa = 4.05KK148 pKa = 10.78ANSGTAEE155 pKa = 3.98RR156 pKa = 11.84VV157 pKa = 3.17

Molecular weight:
18.35 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2827

157

2002

706.8

81.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.174 ± 0.816

2.335 ± 0.535

5.766 ± 0.805

8.56 ± 0.648

5.624 ± 0.904

5.341 ± 0.929

2.299 ± 0.47

6.969 ± 0.344

9.268 ± 1.519

7.853 ± 0.672

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.042 ± 0.782

6.155 ± 0.748

2.688 ± 0.452

2.618 ± 0.267

5.624 ± 0.426

7.676 ± 0.804

3.714 ± 0.662

6.19 ± 0.587

1.097 ± 0.074

3.007 ± 0.514

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski